1. DNA conformational equilibrium in the presence of Zn2+ ions in neutral and alkaline solutions.
- Author
-
Sorokin VA, Valeev VA, Usenko EL, and Andrushchenko VV
- Subjects
- Absorption, Animals, Hydrogen-Ion Concentration, Solutions, Transition Temperature, DNA chemistry, Nucleic Acid Conformation drug effects, Zinc pharmacology
- Abstract
Effect of Zn(2+) ions on DNA transition from B-form to a metallized form (m-DNA) in Tris and tetraborate buffers at pH 8.5 has been studied by visible and differential UV-spectroscopy and by thermal denaturation. The results have been compared to those obtained at pH 6.5 in cacodylate buffer. It was found that in alkaline solutions Zn(2+) ions induced a hypochromicity of the DNA absorption in the whole spectral range monitored, which was attributed to DNA transition from B- to the m-form. Complete metallization occurred only upon heating the DNA solutions containing more than ~2×10(-4) M of Zn(2+) ions. Phase diagrams of the DNA-zinc complexes at pH 6.5 and 8.5 have been obtained for the first time. The m-DNA form showed higher thermal stability compared to B-DNA., (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Published
- 2012
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