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DNA conformational equilibrium in the presence of Zn2+ ions in neutral and alkaline solutions.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2012 Apr 01; Vol. 50 (3), pp. 854-60. Date of Electronic Publication: 2011 Nov 22. - Publication Year :
- 2012
-
Abstract
- Effect of Zn(2+) ions on DNA transition from B-form to a metallized form (m-DNA) in Tris and tetraborate buffers at pH 8.5 has been studied by visible and differential UV-spectroscopy and by thermal denaturation. The results have been compared to those obtained at pH 6.5 in cacodylate buffer. It was found that in alkaline solutions Zn(2+) ions induced a hypochromicity of the DNA absorption in the whole spectral range monitored, which was attributed to DNA transition from B- to the m-form. Complete metallization occurred only upon heating the DNA solutions containing more than ~2×10(-4) M of Zn(2+) ions. Phase diagrams of the DNA-zinc complexes at pH 6.5 and 8.5 have been obtained for the first time. The m-DNA form showed higher thermal stability compared to B-DNA.<br /> (Copyright © 2011 Elsevier B.V. All rights reserved.)
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 50
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 22119960
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2011.11.011