1. Analysis of histone modifications in mouse neocortical neural progenitor-stem cells at various developmental stages.
- Author
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Tsuboi M and Gotoh Y
- Subjects
- Animals, Cell Culture Techniques instrumentation, Cell Culture Techniques methods, Cells, Cultured, Chromatin Immunoprecipitation instrumentation, Female, Gene Expression Regulation, Developmental, Mice, Inbred C57BL, Mice, Inbred ICR, Neural Stem Cells cytology, Pregnancy, Real-Time Polymerase Chain Reaction methods, Ubiquitination, Mice, Chromatin Immunoprecipitation methods, Histones metabolism, Neocortex cytology, Neocortex embryology, Neural Stem Cells metabolism
- Abstract
Dynamic changes in histone modifications mediated by Polycomb group proteins can be indicative of the transition of gene repression mode during development. Here, we present methods for the isolation of mouse neocortical neural progenitor-stem cells (NPCs) and their culture, followed by chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) techniques to examine changes in histone H2A ubiquitination patterns at various developmental stages. This protocol can be applied for both in vitro NPCs and NPCs directly isolated from mouse neocortices. For complete details on the use and execution of this protocol, please refer to (Tsuboi et al., 2018)., Competing Interests: The authors declare no competing interests., (© 2021 The Authors.)
- Published
- 2021
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