1. The Response of Pseudomonas aeruginosa PAO1 to UV-activated Titanium Dioxide/Silica Nanotubes.
- Author
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Augustyniak A, Cendrowski K, Grygorcewicz B, Jabłońska J, Nawrotek P, Trukawka M, Mijowska E, and Popowska M
- Subjects
- Bacterial Outer Membrane Proteins genetics, Biofilms drug effects, Biofilms growth & development, Drug Resistance, Multiple drug effects, Drug Resistance, Multiple genetics, Gene Expression Regulation, Bacterial drug effects, Humans, Membrane Transport Proteins genetics, Microbial Sensitivity Tests, Microscopy, Electron, Transmission, Nanocomposites radiation effects, Nanocomposites ultrastructure, Nanotubes ultrastructure, Pseudomonas Infections microbiology, Pseudomonas aeruginosa genetics, Pseudomonas aeruginosa physiology, Spectrometry, X-Ray Emission, Ultraviolet Rays, X-Ray Diffraction, Nanocomposites administration & dosage, Nanotubes chemistry, Pseudomonas aeruginosa drug effects, Silicon Dioxide chemistry
- Abstract
Pseudomonas aeruginosa is a bacterium of high clinical and biotechnological importance thanks to its high adaptability to environmental conditions. The increasing incidence of antibiotic-resistant strains has created a need for alternative methods to increase the chance of recovery in infected patients. Various nanomaterials have the potential to be used for this purpose. Therefore, we aimed to study the physiological response of P. aeruginosa PAO1 to titanium dioxide/silica nanotubes. The results suggest that UV light-irradiated nanomaterial triggers strong agglomeration in the studied bacteria that was confirmed by microscopy, spectrophotometry, and flow cytometry. The effect was diminished when the nanomaterial was applied without initial irradiation, with UV light indicating that the creation of reactive oxygen species could play a role in this phenomenon. The nanocomposite also affected biofilm formation ability. Even though the biomass of biofilms was comparable, the viability of cells in biofilms was upregulated in 48-hour biofilms. Furthermore, from six selected genes, the mexA coding efflux pump was upregulated, which could be associated with an interaction with TiO
2 . The results show that titanium dioxide/silica nanotubes may alter the physiological and metabolic functions of P. aeruginosa PAO1.- Published
- 2020
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