1. The siRNAsome: A Cation-Free and Versatile Nanostructure for siRNA and Drug Co-delivery.
- Author
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Zheng M, Jiang T, Yang W, Zou Y, Wu H, Liu X, Zhu F, Qian R, Ling D, McDonald K, Shi J, and Shi B
- Subjects
- Antibiotics, Antineoplastic chemistry, Breast Neoplasms metabolism, Breast Neoplasms pathology, Cell Proliferation drug effects, Cell Survival drug effects, Doxorubicin chemistry, Drug Resistance, Multiple drug effects, Drug Screening Assays, Antitumor, Humans, Hydrophobic and Hydrophilic Interactions, MCF-7 Cells, Antibiotics, Antineoplastic pharmacology, Breast Neoplasms drug therapy, Doxorubicin pharmacology, Drug Delivery Systems, Nanostructures chemistry, RNA, Small Interfering chemistry
- Abstract
Nanoparticles show great potential for drug delivery. However, suitable nanostructures capable of loading a range of drugs together with the co-delivery of siRNAs, which avoid the problem of cation-associated cytotoxicity, are lacking. Herein, we report an small interfering RNA (siRNA)-based vesicle (siRNAsome), which consists of a hydrophilic siRNA shell, a thermal- and intracellular-reduction-sensitive hydrophobic median layer, and an empty aqueous interior that meets this need. The siRNAsome can serve as a versatile nanostructure to load drug agents with divergent chemical properties, therapeutic proteins as well as co-delivering immobilized siRNAs without transfection agents. Importantly, the inherent thermal/reduction-responsiveness enables controlled drug loading and release. When siRNAsomes are loaded with the hydrophilic drug doxorubicin hydrochloride and anti-P-glycoprotein siRNA, synergistic therapeutic activity is achieved in multidrug resistant cancer cells and a tumor model., (© 2019 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.)
- Published
- 2019
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