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Start Over Author "Maserti, B. E." Topic n-alkanes
3 results on '"Maserti, B. E."'

2. Expression and characterization of two new alkane-induciblecytochrome P450s from Trichoderma harzianum

Author

Del Carratore R., Gervasi P. G., Contini M. P., Beffy P., Maserti B. E., Giovannetti G., Brondolo A., and Longo V.

Subjects
n-Alkanes, Filamentous fungi, Trichoderma harzianum, Cytochrome P450, Fatty acids
Abstract

n-Dodecane and fatty acids were good inducers of cytochrome P450 (CYP) and the x-hydroxylase of lauric acid, which is a marker for x-hydroxylation of n-alkanes, in Trichoderma harzianum. A cDNA, containing an ORF of 1520 bp, encoding a CYP52 of 520 amino acids, was isolated by RACE. Another n-alkane-inducible CYP was identified by LLC-MS/MS analysis of a microsomal protein band induced by n-dodecane in a library of T. harzianum. This suggests that T. harzianum has a CYP-dependent conversion of alkanes to fatty acids allowing their incorporation into lipids.

Published
2011

3. Expression and characterization of two new alkane-inducible cytochrome P450s from Trichoderma harzianum.

Author

del Carratore, R., Gervasi, P. G., Contini, M. P., Beffy, P., Maserti, B. E., Giovannetti, G., Brondolo, A., and Longo, V.

Subjects
GENE expression, CYTOCHROME P-450, ALKANES, CYTOCHROMES, TRICHODERMA, FATTY acids, LIPIDS
Abstract

n-Dodecane and fatty acids were good inducers of cytochrome P450 (CYP) and the ω-hydroxylase of lauric acid, which is a marker for ω-hydroxylation of n-alkanes, in Trichoderma harzianum. A cDNA, containing an ORF of 1520 bp, encoding a CYP52 of 520 amino acids, was isolated by RACE. Another n-alkane-inducible CYP was identified by LLC-MS/MS analysis of a microsomal protein band induced by n-dodecane in a library of T. harzianum. This suggests that T. harzianum has a CYP-dependent conversion of alkanes to fatty acids allowing their incorporation into lipids. [ABSTRACT FROM AUTHOR]

Published
2011
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