Expression and characterization of two new alkane-induciblecytochrome P450s from Trichoderma harzianum

Authors :: Del Carratore R.
Gervasi P. G.
Contini M. P.
Beffy P.
Maserti B. E.
Giovannetti G.
Brondolo A.
Longo V.
Source :: Biotechnology letters 33 (2011): 1201–1206., info:cnr-pdr/source/autori:Del Carratore R., Gervasi P. G., Contini M. P., Beffy P., Maserti B. E., Giovannetti G., Brondolo A., Longo V./titolo:Expression and characterization of two new alkane-induciblecytochrome P450s from Trichoderma harzianum/doi:/rivista:Biotechnology letters/anno:2011/pagina_da:1201/pagina_a:1206/intervallo_pagine:1201–1206/volume:33
Publication Year :: 2011
Publisher :: Science and Technology Letters, Kew (12 Clarence Rd, Kew, Surrey TW9 3NL) , Regno Unito, 2011.
Abstract: n-Dodecane and fatty acids were good inducers of cytochrome P450 (CYP) and the x-hydroxylase of lauric acid, which is a marker for x-hydroxylation of n-alkanes, in Trichoderma harzianum. A cDNA, containing an ORF of 1520 bp, encoding a CYP52 of 520 amino acids, was isolated by RACE. Another n-alkane-inducible CYP was identified by LLC-MS/MS analysis of a microsomal protein band induced by n-dodecane in a library of T. harzianum. This suggests that T. harzianum has a CYP-dependent conversion of alkanes to fatty acids allowing their incorporation into lipids.

Subjects :: n-Alkanes
Filamentous fungi
Trichoderma harzianum
Cytochrome P450
Fatty acids

Database :: OpenAIRE
Journal :: Biotechnology letters 33 (2011): 1201–1206., info:cnr-pdr/source/autori:Del Carratore R., Gervasi P. G., Contini M. P., Beffy P., Maserti B. E., Giovannetti G., Brondolo A., Longo V./titolo:Expression and characterization of two new alkane-induciblecytochrome P450s from Trichoderma harzianum/doi:/rivista:Biotechnology letters/anno:2011/pagina_da:1201/pagina_a:1206/intervallo_pagine:1201–1206/volume:33
Accession number :: edsair.cnr...........5fe8dcd38739880f6a261f5c761fe965

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