1. Inactivation of bacteriophages T7 and phiX174 by radiomimetics.
- Author
-
Rontó G, Smotryaeva M, Kruglyakova K, Emanuel N, and Tarján I
- Subjects
- Caffeine pharmacology, Cell Survival drug effects, Coliphages drug effects, DNA Viruses drug effects, DNA Viruses metabolism, Escherichia coli drug effects, Kinetics, Mathematics, Azirines pharmacology, Coliphages metabolism, Methylnitrosourea pharmacology, Nitrosourea Compounds pharmacology
- Abstract
Inactivation of phages T7 and phiX174 induced by the radiomimetics N-nitroso-N-methylurea (NMU) and N-acetylethylenimine (AEI) has been studied. In semilogarithmic scale the kinetic curves of phage inactivation were steplike in character. This was connected with the occurrence of several processes in the system. A possible kinetic model has been proposed for the process of NMU-induced phage inactivation. The model is applicable to initial phage inactivation only. From the model a kinetic curve parameter, rho can be determined which characterizes the radiomimetic effect on different phages and determines the changes in the repair system after caffeine treatment. NMU exerted a post-inactivation effect on the phage T7. Also the NMU-induced phage T7 post-inactivation kinetic curves were of steplike character. They can be described as a sum of two exponential processes of different rate constant. The rate constant for the first stage of the post-inactivation process was close to the depurinization rate constant found in the literature.
- Published
- 1975