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Inactivation of bacteriophages T7 and phiX174 by radiomimetics.
- Source :
-
Acta biochimica et biophysica; Academiae Scientiarum Hungaricae [Acta Biochim Biophys Acad Sci Hung] 1975; Vol. 10 (1-2), pp. 115-22. - Publication Year :
- 1975
-
Abstract
- Inactivation of phages T7 and phiX174 induced by the radiomimetics N-nitroso-N-methylurea (NMU) and N-acetylethylenimine (AEI) has been studied. In semilogarithmic scale the kinetic curves of phage inactivation were steplike in character. This was connected with the occurrence of several processes in the system. A possible kinetic model has been proposed for the process of NMU-induced phage inactivation. The model is applicable to initial phage inactivation only. From the model a kinetic curve parameter, rho can be determined which characterizes the radiomimetic effect on different phages and determines the changes in the repair system after caffeine treatment. NMU exerted a post-inactivation effect on the phage T7. Also the NMU-induced phage T7 post-inactivation kinetic curves were of steplike character. They can be described as a sum of two exponential processes of different rate constant. The rate constant for the first stage of the post-inactivation process was close to the depurinization rate constant found in the literature.
Details
- Language :
- English
- Volume :
- 10
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Acta biochimica et biophysica; Academiae Scientiarum Hungaricae
- Publication Type :
- Academic Journal
- Accession number :
- 1096520