1. Citral modulates virulence factors in methicillin-resistant Staphylococcus aureus
- Author
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Regiane Yatsuda, Camila Dutra Barbosa, Thamara Louisy Santos Brito, Ana Paula Trovatti Uetanabaro, Jéssica Bomfim de Almeida, Hellen Braga Martins Oliveira, Lucas Miranda Marques, Caline Novais Teixeira Oliveira, Palloma Porto Almeida, Guilherme Barreto Campos, Jully Chayra Santos Viana, Mariana Morais Teixeira, Suzi P. de Carvalho, Jorge Timenetsky, Yasmin Monara Ferreira de Sousa Andrade, Hanna Izadora Laís Novaes Coelho, Manoel Neres Santos Junior, Nathan das Neves Selis, Letícia de Oliveira da Silva, Rafaela de Souza Bittencourt, Beatriz Almeida Sampaio, and Icaro Bonyek Santos da Silva
- Subjects
Methicillin-Resistant Staphylococcus aureus ,medicine.drug_class ,Virulence Factors ,Science ,Acyclic Monoterpenes ,Antibiotics ,Virulence ,Microbial Sensitivity Tests ,Biology ,medicine.disease_cause ,Citral ,Microbiology ,Article ,Minimum inhibitory concentration ,chemistry.chemical_compound ,medicine ,Multidisciplinary ,Minimum bactericidal concentration ,Microscopy, Confocal ,biochemical phenomena, metabolism, and nutrition ,Antimicrobial ,Methicillin-resistant Staphylococcus aureus ,Anti-Bacterial Agents ,chemistry ,Staphylococcus aureus ,Biofilms ,Medicine ,Pathogens ,REAÇÃO EM CADEIA POR POLIMERASE - Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for high morbidity and mortality rates. Citral has been studied in the pharmaceutical industry and has shown antimicrobial activity. This study aimed to analyze the antimicrobial activity of citral in inhibiting biofilm formation and modulating virulence genes, with the ultimate goal of finding a strategy for treating infections caused by MRSA strains. Citral showed antimicrobial activity against MRSA isolates with minimum inhibitory concentration (MIC) values between 5 mg/mL (0.5%) and 40 mg/mL (4%), and minimum bactericidal concentration (MBC) values between 10 mg/mL (1%) and 40 mg/mL (4%). The sub-inhibitory dose was 2.5 mg/mL (0.25%). Citral, in an antibiogram, modulated synergistically, antagonistically, or indifferent to the different antibiotics tested. Prior to evaluating the antibiofilm effects of citral, we classified the bacteria according to their biofilm production capacity. Citral showed greater efficacy in the initial stage, and there was a significant reduction in biofilm formation compared to the mature biofilm. qPCR was used to assess the modulation of virulence factor genes, and icaA underexpression was observed in isolates 20 and 48. For icaD, seg, and sei, an increase was observed in the expression of ATCC 33,591. No significant differences were found for eta and etb. Citral could be used as a supplement to conventional antibiotics for MRSA infections.
- Published
- 2020