1. Effect of bone sialoprotein on proliferation and osteodifferentiation of human bone marrow-derived mesenchymal stem cells in vitro
- Author
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Lida Guo, Zujun Jiang, Bing Xia, and Jie Wang
- Subjects
Bone sialoprotein ,medicine.medical_specialty ,Sialoglycoproteins ,Bone Marrow Cells ,Bioengineering ,Applied Microbiology and Biotechnology ,fluids and secretions ,stomatognathic system ,Internal medicine ,medicine ,Humans ,Dexamethasone ,Cell Proliferation ,DNA Primers ,Pharmacology ,Base Sequence ,General Immunology and Microbiology ,biology ,Cell growth ,Mesenchymal stem cell ,Cell Differentiation ,Mesenchymal Stem Cells ,General Medicine ,Ascorbic acid ,In vitro ,Endocrinology ,biology.protein ,Osteocalcin ,Alkaline phosphatase ,Biotechnology ,medicine.drug - Abstract
We performed this study to investigate the effects of recombinant human bone sialoprotein (BSP) on the proliferation and osteodifferentiation of human BMSCs(hBMSCs). The hBMSC cultures were divided into 4 groups: control group, 10(-10) M BSP group (BSP group), osteogenic medium group (10 nM dexamethasone, 10 mM β-glycerophosphate, and 50 mg/L ascorbic acid, OM group) and BSP + OM group (OM plus10(-10) M BSP). Compared with the control group, cell growth of the other three groups slowed down, while fluorescence at the G(0)/G(1) phase increased. After 28 days, in the OM group and the BSP + OM group, the proportion of STRO-1-positive cells decreased by 22.7% and 38.4% and ALP activity increased by 50% and 71.43%, respectively. CD271 mRNA expression decreased while Cbfa1, osteocalcin and osterix mRNA levels increased in the OM and BSP + OM groups, and the mRNA level change was greater in the BSP + OM group. After 28 days, the number of nodules in the BSP + OM group was 112.5% more than that in the OM group, but nodules did not formed in the control or BSP group. We conclude that BSP is capable of inhibiting hBMSCs proliferation and enhancing their osteogenic differentiation and mineralization in the presence of OM.
- Published
- 2011