1. Comparison of the effects of octreotide and melatonin in preventing nerve injury in rats with experimental spinal cord injury
- Author
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Cahide Topsakal, Ibrahim Hanifi Ozercan, Huseyin Yakar, Metin Kaplan, Murat Tiftikci, Fatih Serhat Erol, and Necip Ilhan
- Subjects
Male ,medicine.medical_specialty ,Necrosis ,medicine.medical_treatment ,Urology ,Octreotide ,Antioxidants ,Drug Administration Schedule ,Melatonin ,Superoxide Dismutase-1 ,Glutathione Peroxidase GPX1 ,Gastrointestinal Agents ,Malondialdehyde ,Physiology (medical) ,Edema ,medicine ,Animals ,Rats, Wistar ,Spinal cord injury ,Spinal Cord Injuries ,Glutathione Peroxidase ,Dose-Response Relationship, Drug ,Superoxide Dismutase ,business.industry ,Laminectomy ,General Medicine ,Nerve injury ,medicine.disease ,Rats ,Disease Models, Animal ,Oxidative Stress ,Dose–response relationship ,Neuroprotective Agents ,Treatment Outcome ,Neurology ,Anesthesia ,Nerve Degeneration ,Surgery ,Neurology (clinical) ,medicine.symptom ,Wallerian Degeneration ,business ,Injections, Intraperitoneal ,medicine.drug - Abstract
In this study, we aimed to investigate the biochemical and histopathological protective effects of octreotide and melatonin in an experimental model of spinal cord injury. Fifty- six male albino Wistar rats were divided into four groups. Rats in the G1 group (n=7; control group) did not undergo any treatment except for anesthesia prior to being killed. Rats in the G2 group (n=7) underwent laminectomy and aneurysmal clip application at the T4-5 level. G3 group rats (n=14) were either treated with a 7.5 mg/kg intraperitoneal dose of melatonin (Sigma, St. Louis, MO, USA) immediately after laminectomy, then the same dose again on the day following injury (G3a), or given three equal doses over 10 days to achieve a total dose of 7.5 mg/kg/day (G3b). G4 group rats (n=14) were either treated with a 30microg/kg intraperitoneal dose of octreotide (Sandostatin; Novartis, Istanbul, Turkey) immediately after laminectomy, then the same dose again on the day following injury (G4a), or given three equal doses over 10 days to achieve a total dose of 30miocrog/kg/day (G4b). Rats in the G3 and G4 groups were sacrificed on days 1 and 10 after spinal cord injury (n=7 at each time point) and spinal cord samples were obtained. Tissue malonyldialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) levels were assayed. G3a, G3b and G4b had significantly lower levels of MDA than G2 (p
- Published
- 2008