1. Photoreceptor precursor cell integration into rodent retina after treatment with novel glycopeptide PKX‐001
- Author
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Ishaq A. Viringipurampeer, Kevin Gregory-Evans, Vahitha Shameem Nizamudheen, Anat Yanai, and Cheryl Y. Gregory-Evans
- Subjects
Male ,Retinal degeneration ,Opsin ,Cell Survival ,0206 medical engineering ,Biomedical Engineering ,Medicine (miscellaneous) ,02 engineering and technology ,Biology ,Biomaterials ,03 medical and health sciences ,Precursor cell ,Retinitis pigmentosa ,Electroretinography ,medicine ,Animals ,Humans ,030304 developmental biology ,0303 health sciences ,Retina ,Glycopeptides ,medicine.disease ,020601 biomedical engineering ,Rats ,Cell biology ,Transplantation ,medicine.anatomical_structure ,Female ,sense organs ,Stem cell ,Ex vivo ,Photoreceptor Cells, Vertebrate - Abstract
Cell replacement therapy is emerging as an important approach in novel treatments for neurodegenerative diseases. Many problems remain, in particular improvements are needed in the survival of transplanted cells and increasing functional integration into host tissue. These problems arise because of immune rejection, suboptimal precursor cell type, trauma during cell transplantation, and toxic compounds released by dying tissues and nutritional deficiencies. We recently developed an ex vivo system to facilitate identification of factors contributing to the death of transplanted neuronal (photoreceptor) and showed 2.8-fold improvement in transplant cell survival after pretreatment with a novel glycopeptide (PKX-001). In this study, we extended these studies to look at cell survival, maturation, and functional integration in an in vivo rat model of rhodopsin-mutant retinitis pigmentosa causing blindness. We found that only when human photoreceptor precursor cells were preincubated with PKX-001 prior to transplantation, did the cells integrate and mature into cone photoreceptors expressing S-opsin or L/M opsin. In addition, ribbon synapses were observed in the transplanted cells suggesting they were making synaptic connections with the host tissue. Furthermore, optokinetic tracking and electroretinography responses in vivo were significantly improved compared to cell transplants without PKX-001 pre-treatment. These data demonstrate that PKX-001 promotes significant long-term stem cell survival in vivo, providing a platform for further investigation towards the clinical application to repair damaged or diseased retina.
- Published
- 2021