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Treating the signs of skin ageing or acne scars by chemical peels or ablative lasers is increasingly used worldwide. Due to their reduced invasiveness, these methods are often favored over aesthetic surgical interventions. Both procedures rely on the principle of controlled damage to the skin. The subsequent regeneration may lead to the development of new epidermal (and dermal) tissue and therefore improve the skin's aesthetic appearance. At present, there are no official guidelines in Germany concerning pre- and post-interventional skin care, which is currently based on the personal experiences and evaluations of the practitioner. It is known that an appropriate treatment regime can improve the outcome and reduces downtime pre- and post-laser as well as pre- and post-peel. The aim of this article is to present the different possibilities of pre- and post-interventional care. In most cases, priming includes intense ultraviolet (UV) protection, topical retinoids as well as skin brightening agents, and occasionally oral herpes simplex prophylaxis. In order to support post-interventional wound healing, skin care should modulate inflammation and balance skin hydration. In addition to light moisturizers, broad spectrum UV protection as well as the avoidance of sports and sweating are essential.

Background: Injection of autologous adipose-derived stem cells (ADSCs) and a stromal vascular fraction (VSF) into dermal and subdermal layers promises regenerative advantages by improving skin volume and rejuvenation. Injectable hyaluronic acid (HA) is a temporary dermal filler that, by improving skin hydration, reduces the appearance of fine lines and wrinkles, facial folds and creates structure and volume to the face and lips. This study combined the grafting of micro fragmented fatty tissue with the hyaluronic acid filler procedure, using three different types of HA. Methods: Each sample of micro fragmented adipose tissue harvested using the superficial enhanced fluid fat injection (SEFFI) technique collected from 8 patients were equally divided into two specimens. One of these (EMU specimens) was emulsified by gently applying ten back-and-forth passages from one syringe to another to fluidify the tissue. The other one was not emulsified (Ctrl/NON-EMU specimen). Both EMU and NON-EMU specimens were divided into four aliquots: one served as control, and the others were combined with each of three tested hyaluronic acids. Afterward, we assessed the cellularity of mesenchymal phenotype (defined as the number of adherent cells with mesenchymal phenotype per milliliter of adipose tissue) and the in vitro capacity of differentiation in mesenchymal lineages. Results: Despite low cellularity from emulsified samples combined with HA, isolated cells could grow and expand in culture, thus proving their proliferative ability, showing “good quality” in all conditions (Ctrl/NON-EMU, EMU, and combined with HA). The cells could differentiate towards mesenchymal lineages, express mesenchymal markers by flow cytometry analysis, and maintain their stemness potential. Conclusion: The combination of emulsified harvested tissue with HA products can be exploited to counteract the loss of volume and skin aging of the human face and body. This approach to regenerative aesthetic treatment is a promising treatment for facial antiaging therapy.

Recently, a novel humidifier that sprays water fine droplets equipped with a copolymer, poly(3,4-ethylene dioxythiophene)-poly(styrene sulfonate) (PEDOT/PSS) was developed. PEDOT/PSS in the humidifier absorbs water from the environment and releases fine water droplets by heating. In the present study, the effect of hydration on the skin barrier, stratum corneum, was first determined by the application of fine water droplets using the humidifier. The skin-penetration enhancement effect of a model hydrophilic drug, caffeine, was also investigated using the humidifier and compared with a conventional water-evaporative humidifier. More prolonged skin hydration effect was observed after application of the fine water droplet release humidifier using PEDOT/PSS than that using a conventional humidifier. In addition, markedly higher skin permeation of caffeine was observed in both infinite and finite dose conditions. Furthermore, higher skin permeation of caffeine from oil/water emulsion containing caffeine was observed in finite dose conditions by pretreatment with the humidifier using PEDOT/PSS. This device can provide water droplets without replenishing water, so it is more convenient for enhancing the skin permeation of chemical compounds from topical drugs and cosmetic formulations.

“Soak and smear” method, water soaking to induce skin hydration followed by topical corticoids application suggests effectiveness in clinical dermatological practice. We investigate one possible me...

Introdução: Na atualidade, a hidratação cutânea é fundamental para a manutenção da aparência jovem, saudável e viçosa das mulheres. O processo de hidratação dos tecidos é executado pelas funções do nosso próprio organismo, juntamente com a água ingerida. A água é responsável por promover a hidratação da pele através das camadas inferiores – da derme para epiderme – sendo transportadas através dos queratinócitos pelos canais proteicos. Estes canais responsáveis pelo transporte de água pelas camadas da pele são conhecidos como aquaporinas 3, que se trata de uma proteína, estando presente na membrana plasmática dos queratinócitos. Objetivo: Este trabalho teve como objetivo realizar uma revisão da literatura sobre os benefícios das Aquaporinas 3 aplicadas a cosmetologia em região de face de mulheres. Método: Para tal objetivo, foi elaborado um estudo com base em artigos científicos, a partir de 2010, com bases de dados eletrônicos PubMed e Google Acadêmico, onde foram consultados artigos originais e de revisão sobre o tema Aquaporinas. Resultados: A partir da revisão realizada verificou-se que a descoberta dos canais de aquaporinas 3 trouxe importantes contribuições para compreender os mecanismos envolvidos na hidratação da pele, proporcionado um aspecto saudável através da passagem de água mediada por esses poros. Evidenciou-se, também, que houve um grande avanço tecnológico, existindo a necessidade de desenvolver princípios ativos que atuam na manutenção destes canais aumentando a expressão e as atividades das aquaporinas 3 na membrana celular, restaurando a hidratação da pele e revitalizando-a. Conclusão: Observou-se que as aquaporinas 3 contribuem de maneira significativa para a hidratação da pele, porém se faz-se necessário o uso de ativos que favorecem seu aparecimento nas células cutâneas, melhorando a hidratação devido o envelhecimento cutâneo.

Background: Handwashing using soap or hand sanitizer is one of the health protocols that must be implemented by society to prevent the spreading of SARS-COV2 virus which can cause pneumonia, acute respiratory syndrome and death. The implementation of health protocols during the COVID-19 pandemic caused the habit of handwashing increase. Hence, it can lead to cause irritant contact dermatitis and dry hand skin due to frequent contact with soap or hand sanitizers. Method: This study is an observational study with the sample from the villager of Banyumas regency. The data collection was taken by cluster random sampling and accidental sampling. Results: Considering the health protocol, most of the villager used to wash their hands regularly and 3 respondents (3.00%) of them did not. 100 respondents took the data and 19 respondents (19.00%) have irritant contact dermatitis and the rest did not. Furthermore, for skin hydration levels with dehydration category (0% - 29%) are 50 respondents (50.00%), normal category (30% - 50%) are 38 respondents (38.00%) and hydration category (51% - 100 %) are 12 respondents (12.00%). Chi-Square analysis showed that there was no significant relationship between handwashing habit and the incidence of irritant contact dermatitis with the value of “P” is 0.394> 0.05 and there was a significant relationship between hand washing habits and skin hydration levels with the value of “P” is 0.010

Background Silicon is an abundant element in the human body and plays an important role in the skin, taking part in the synthesis of collagen and glycosaminoglycans. The use of nanotechnology methods, which processes materials at an atomic and molecular scale, has allowed the development of nanosilicons. Aims The study evaluates the effectiveness of a food supplement and a topical solution containing nanosilicon in improving skin hydration and elasticity. Methods A total of 30 female subjects were randomized to receive the placebo (n = 15) and the active compound (n = 15). All enrolled subjects took the food supplement twice a day for 20 days and then once a day for the next 20 days, and they also had to apply the nanosilicon solution on the right forearm four times a day. Evaluation of several parameters was performed after 20 and 40 days through the use of non-invasive instrumental methods (Corneometer® CM 825, Cutometer® MPA 580, Visioscan® VC, Tewameter® TM 200). Results Both treatment groups showed a statistically significant improvement in barrier function and in skin hydration on the right forearm after 20 days; increase in skin elasticity was observed only in the group taking the active compound. Conclusions The study showed that the administration of a food supplement and a topical solution, both containing nanosilicon, improves hydration, elasticity, and skin barrier function.

Background Dry skin, caused by improper care or genetic conditions, can affect people of all ages. Skin hydration is determined its lipid content, which inhibits water loss from the epidermis, as well as other substances such as polyhydroxy acids and gluconolactone that can bind water. The aim of this study was to evaluate skin hydration after the application of 10% and 30% gluconolactone solution in a split face model. Materials and methods Sixteen healthy women were qualified for the study. Three split face treatments were performed, with 10% and 30% gluconolactone solution applied to two sides of the face. Skin moisture was measured before each treatment and a week after the last treatment at three measurement sites on either side of the face, that is, on the forehead, around the eye and on the cheek. Results Corneometric measurements showed a significant increase in facial skin hydration after gluconolactone treatment. No significant differences were observed between the application of 10% and 30% solution. Conclusion Gluconolactone is a moisturizing substance which works well in dry skin care.

Background M89 M (Mineral 89 mask, Laboratoires Vichy, France), containing 89% Vichy volcanic mineralizing water and hyaluronic acid, aims to strengthen and repair skin barrier. Aims To assess the efficacy, tolerance, patient satisfaction, and quality of life (QOL) using M89 M after laser procedures (LP). Methods M89 M was applied immediately post‐LP for 10 minutes, then daily for 5 days and 2‐3 times a week, up to 28 days on the faces of 51 women. Evaluations were performed immediately post‐LP, immediately after M89 M application at D0, D1, D5, and D28, and included criteria such as erythema and skin dryness. Subjects scored burning and warm sensations, itching, skin tightness, and stinging. Skin hydration using a Corneometer, skin barrier integrity using a Tewameter, and erythema using a Chromameter were assessed. Local tolerance and adverse events were recorded. After 28 days, subjects answered a questionnaire regarding the M89 M subjective cosmetic properties and QOL. Results All subjects were in their mid‐forties with a phototype of II, III, or IV. M89 M significantly (P

Background: The growing field of psycho-dermatology is all about the mind-skin connection. It addresses the link between our emotional state, the body’s physiological response to our emotional state, and the outcome of this response on our skin, body, health, and well-being. The aim of this clinical trial is to evaluate safety and efficacy of BP-G01 in improving skin health, complexion and skin luster. Material and methods: Total 33 subjects were screened and out them 3 were considered as drop outs and 30 completed subjects were involved in the study. Natural plant based skincare supplement (BP-G01) contains Plant based protein blend like Plant proteins like rice and pea proteins. The glow blend like evening primrose extract, pearl powder, orange, pomegranate, grape seed extract, sea buckthorn extract and tocopherols from plant source. The adaptogenic blend like Ashwagandha, Amla, Tulsi, Mulethi and Turmeric extracts. It was consumed as 90 days’ treatment in dose of 20 gm in the morning after breakfast or post workout with 200 ml of water or milk. Natural plant based skincare supplement (BP-G01) improve improved skin luminosity, brightness, radiance and complexion, skin hydration, skin discoloration, dark circles, dark spots from face and neck and skin evenness indicated by MASI scoring for reducing hyper pigmentation, dark spots/ circles and redness and reduction in mild acne counts. VAS for the improvements in skin complexion and evenness. C.L.B.T. imperfections scale. Result: There was 17.08, 22.79, 32.56 and 37.96% reduction in the MASI score at day 15, 30, 60 and day 90 respectively by reducing hyper pigmentation, dark spots/ circles and redness by MASI scoring. MASI acne score at baseline was 9.10 ± 1.19 which was decreased throughout the period of treatment by reduction in mild acne counts as per modified MASI scoring. Conclusion: BP-G01 is safe and effective in promoting skin health in healthy adults.

Background and Objective: Although compression therapy is a very effective therapy in reducing stasis-induced complaints, the wearing comfort is not always as requested. Most frequent problems are dryness of the skin and itching. This randomized, cross-over trial investigated 33 healthy participants and compared 2 different medical compression stockings: conventional stockings (type A = MCS) versus compression stockings with integrated care emulsion (type B = MCS-SkC). Methods: Participants were divided into 2 cohorts. Both compression types were worn one after the other, 1 week each. The cohorts were named according to the sequence of the wearing periods (cohort AB/BA). Primary outcome: skin hydration. Secondary outcomes: transepidermal water loss (TEWL), skin roughness, leg volume, interface pressure, and questionnaires about stasis-induced complaints and wearing comfort. Results: Skin hydration: significant reduction after wearing MCS in both cohorts ( p < 0.001); preservation of skin moisture after wearing MCS-SkC ( p = 0.546 and p = 0.1631). TEWL: significant increase after wearing MCS ( p = 0.007 and p = 0.0031); significant reduction by wearing MCS-SkC ( p = 0.006 and p = 0.0005). S kin roughness: significant increase after wearing MCS ( p = 0.0015 and p = 0.010), and nonsignificant decrease of skin roughness after wearing MCS-SkC ( p = 0.933 and p = 0.4570). Leg volume: significantly reduced with both stockings ( p = 0.004 and p = 0.0047). Regarding stasis-induced complaints, both stockings achieved good results. Conclusions: Both compression stockings are appropriate to reduce leg edema and minimize leg symptoms. MCS-SkC helps to obtain the natural skin barrier function in preserving the epidermal water content and reducing the TEWL.

Contains fulltext : 235374.pdf (Publisher’s version ) (Open Access) BACKGROUND: Stratum corneum hydration (SCH) and transepidermal water loss (TEWL) provide useful information about skin barrier function. This study aimed to determine the value of GPSkin Pro, a new handheld device determining both SCH and TEWL, to measure skin barrier impairment and to monitor barrier function in rosacea in daily practice. MATERIALS AND METHODS: Two pilots were performed. Pilot 1: in 27 healthy participants, GPSkin SCH and TEWL were compared to Aquaflux(®) and Epsilon(®) values at the forearm before and after skin barrier perturbation via tapestripping. Moreover, GPSkin values were measured at both cheeks without intervention. Pilot 2: in 16 rosacea patients, GPSkin measurements were performed at the forearm, and at both cheeks before and during anti-inflammatory treatment. They were compared to clinical symptoms and to GPSkin values from pilot 1. RESULTS: Pilot 1: after merging data from before and after tapestripping, a strong correlation was observed between GPSkin TEWL and Aquaflux(®) (R(s) = 0.9256), and GPSkin SCH and Epsilon(®) (R(s) = 0.8798). Pilot 2: SCH was significantly lower at the cheeks of rosacea patients compared to controls, with a normalizing trend during successful treatment. TEWL was comparable among patients and controls and did not change during treatment at all locations. CONCLUSION: The GPSkin determines TEWL and SCH accurately in healthy and impaired skin barrier state and can monitor skin barrier function in rosacea during treatment. The GPSkin device is much more practical compared to previous skin barrier tools when used in clinical practice. Its further validation in other inflammatory skin diseases is recommended.

O envelhecimento tem sido um dos temas mais discutidos na atualidade, e diversas teorias tentam explicar esse processo. Porem, a teoria que envolve os radicais livres e as especies reativas de oxigenio e uma das mais abordadas. O resveratrol (RSV), um importante polifenol presente principalmente na uva e seus derivados, tem sido amplamente pesquisado devido aos seus varios beneficios a saude, incluindo as suas propriedades antioxidantes. Objetivo: Portanto, este trabalho pretendeu descrever a acao antioxidante do RSV, comprovando a sua aplicacao cosmetica no controle do envelhecimento cutâneo facial intrinseco e extrinseco. Metodologia: Trata-se de uma revisao integrativa da literatura, cujas buscas foram realizadas nas bases de dados da LILACS, SciELO e PubMed, utilizando os seguintes descritores: “Resveratrol”, “Antioxidante”, “Envelhecimento da Pele”, “Cosmetico” e “Estetica”. Foram incluidos artigos disponiveis na integra, publicados entre os anos 2016 e 2020, nos idiomas portugues ou ingles. Foram excluidos os artigos que nao apresentaram compatibilidade com o tema, conforme triagem baseada na leitura do resumo, alem de trabalhos de conclusao de curso, dissertacoes e teses. Resultados: Constatou-se que dentre os beneficios do RSV podem ser citados: a prevencao aos danos causados pelos radicais livres e radiacao UV, o auxilio no clareamento da pele, uma acao antiacne, o aumento da hidratacao da pele e o estimulo a producao de colageno. Conclusao: O RSV demonstrou ter eficacia no controle do envelhecimento cutâneo facial intrinseco e extrinseco, podendo ser utilizado, tambem, como clareador da pele, porem, com resultados ainda limitados a poucos ensaios clinicos. Palavras Chave: Antioxidante. Cosmetico. Estetica. Envelhecimento da Pele. Resveratrol. ABSTRACT Aging has been one of the most discussed topics today, and several theories try to explain this process. However, the theory that involves free radicals and reactive oxygen species is one of the most discussed. Resveratrol (RSV), an important polyphenol present mainly in grapes and its derivatives, has been widely researched due to its various health benefits, including its antioxidant properties. Objective: Therefore, this work aimed to describe the antioxidant action of RSV, proving its cosmetic application in the control of intrinsic and extrinsic facial skin aging. Methodology: This is an integrative literature review, whose searches were carried out in the databases of LILACS, SciELO and PubMed, using the following descriptors: "Resveratrol", "Antioxidant", "Skin aging", "Cosmetic" and "Aesthetics". Articles available in full, published between the years 2016 and 2020, in Portuguese or English were included. Articles that did not show compatibility with the topic were excluded, according to screening based on reading the abstract, in addition to course completion papers, dissertations and doctoral thesis. Results: It was found that among the benefits of the RSV can be mentioned the prevention of damage caused by free radicals and UV radiation, the aid in whitening the skin, an anti-acne action, the increase in skin hydration and the stimulation of collagen production. Conclusion: RSV has proven to be effective in controlling intrinsic and extrinsic facial skin aging, and can also be used as a skin lightener, but with results still limited to a few clinical trials. Keywords: Antioxidant. Cosmetic. Aesthetics. Skin aging. Resveratrol.

Aim The purpose of this study was to investigate the degradation of desmocollin-1 (DSC1), a member of the desmosomal cadherin family in patients with diabetes, as well as the factors associated with the suppression of DSC1 degradation. Methods This cross-sectional study included 60 cases of foot callus involving 30 patients with diabetes (DM) and 30 matched volunteers without diabetes (non-DM). DSC1 degradation in samples from debrided calluses was analysed using western blotting. Skin hydration, a factor reported to suppress DSC1 degradation, was measured using a mobile moisture device. Results Full-length DSC1 (approximately 100 kDa) was detected in six participants only in the DM group, and no relationship was found between the suppression of DSC1 degradation and decreased skin hydration in the DM group. There was no significant difference in skin hydration values between the DM and non-DM groups. Conclusion DSC1 degradation was suppressed in the DM group. There was no relationship between the suppression of DSC1 degradation and decreased skin hydration in the DM group. Current external force callus care would not be sufficient. This study highlights the need to develop novel callus care to enhance the degradation of DSC1.

Symptomatology and severity of atopic dermatitis (AD) can be objectively measured with equipment. This study aimed to compare skin measurements and investigate their correlations with various clinical severity scores.Skin hydration (SH), transepidermal water loss (TEWL), pH, erythema, pigmentation, and ITA (individual typology angle) were measured (using Delfin, Courage + Khazaka, and Mettler Toledo equipment), and correlated with Patient-Oriented Eczema Measure (POEM, a short-term subjective-symptom score), Scoring Atopic Dermatitis (SCORAD, a short-term subjective-symptom and objective-sign score), Nottingham Eczema Severity Score (NESS, a long-term subjective-symptom score), Children Dermatology Life Quality Index (CDLQI, a short-term subjective-symptom score) with Spearman's rho coefficient.80 sets of clinical scores from eczema patients (mean age: 10.8 ± 4.9 years; 44.6% male) were evaluated. The POEM, objective SCORAD, CDLQI correlated well with each other. Skin pH ranged from 4.3 to 7.0 (mean 5.7 ± 0.61). Skin pH was correlated with Objective SCORAD components, including area (rho = 0.269,Skin pH (using Mettler Toledo), SH, and TEWL (using Delfin equipment) correlated well with various clinical symptomatology scores. Less acidic pH appears to be associated with worse clinical scores of symptomatology, and increase usage of topical antibiotics, These findings not only support the supplementary usage of equipment in aiding objective documentation of clinical symptomatology in eczema therapeutic research but also the advocacy of maintaining more acidic skin and avoiding alkaline soap and emollient products.

Inadequate xeros is treatment may result in complications. This study aimed to comparing the efficacy and side effects of moisturizing cream containing 10% urea and 10% lanolin in petrolatum for the treatment of xerosis in elderly.A randomized, double blind clinical trial was conducted in 35 elderly from a nursing home in Jakarta. Evaluation of skin capacitance (SC),specified symptoms sum score(SSRC), and pruritic degree were measured at baseline, week-2 and -4 after the start of therapy. Followinga 2-week preconditionperiod, eachsubjectreceivedarandommoisturizer for each limb, to be applied twice daily.Thepercentageof SC increaseatweek-4 was significantly higher in limb receiving cream containing 10%urea than 10% lanolin/petrolatum (64.54% vs. 58.98%; p=0.036). Thepercentage of SSRC decreaseatweek-4 did not differ between groups (100%; p=0.089). Pruritus was equally improved in both groups at week-2, and completely diminishedatweek-4. Overall, the most frequent side effect was sticky feel, more common, but not statistically significant, in 10% lanolin/petrolatum group.Afterfour- week application moisturizer containing 10% urea gave higher percentage of SC increase than 10% lanolin/petrolatum in the xerotic limbs of the elderly. Sticky feeling was more frequently found in 10% lanolin/petrolatum group, butstatisticallynotsignificant.

Background and Aim: Rabbits often experience skin diseases. The beneficial effects of plant extracts and essential oils are well known in other species, but the properties of these natural ingredients have not been evaluated in rabbits in vivo. The objective of the current study was to evaluate the effect of a topical, commercial solution made of essential oils, plant-extracted polyunsaturated fatty acids, and Vitamin E on rabbits suffering from skin problems. Materials and Methods: Thirty New Zealand rabbits (no sex distinction) were included in this study, with an average weight of 2–3 kg. The rabbits were divided into two groups: The first group was treated with a topical solution made from natural ingredients, and the second was a control group. The rabbits' hair and skin conditions were evaluated on days 1, 14, 28, and 35 after treatment. Data were analyzed using a Kruskal–Wallis range test. Results: Significant differences were determined in terms of glossy hair variability on days 28 and 35 (p≤0.0001). On days 14-35, hair loss was determined to have reduced (p=0.001), and flaking and odor improved in the treatment group, showing increased scores and significant differences (p=0.0001). By contrast, the control group showed stable overall skin and hair score and an increase in the dryness score. Conclusion: The topical application of essential oils and polyunsaturated fatty acids with Vitamin E was able to improve hair shine and skin hydration and reduce flaking, bad odor, and hair loss, improving the general, and cutaneous aspect of rabbits.

BACKGROUND The use of light-emitting diode (LED) in combination wavelength for facial rejuvenation has been previously reported. Nowadays, there has been a growing market for home-use cosmetic devices because of its low cost and convenience. AIMS To evaluate the efficacy and safety of home-use LED device on facial rejuvenation. METHODS This was a prospective split-face clinical trial with a total of 24 subjects, who presented with photo aging skin. All subjects were treated with the home-use LED device on the left side of their face, twice a week for 8 weeks. Primary outcomes measured in the study were the changes in the biophysical properties of the skin assessed with the following parameters: skin elasticity, skin hydration, texture, and wrinkles. Evaluations were done at baseline, 2-, 4-, 6-, and 8-week follow-up. Subjects' self-improvement scores and adverse reactions were also recorded. RESULTS All 24 subjects completed the study and attended all follow-up. Skin elasticity was significantly higher in the LED group compared to the control during the 6- and 8-week follow-up (P

Background Oxybrasion is one of the types of microdermabrasion. This method removes a superficial epidermal layer with the use of a stream of 0.9% saline solution and compressed air. Aims The assessment of skin hydration, sebum, pH, TEWL (transepidermal water loss), and pigmentation using oxybrasion. Material and methods The study involved 27 female subjects. Five treatments were performed every 2 weeks using the oxygen microdermabrasion. Measurements of the skin parameters were taken before each treatment and 2 weeks after the last procedure. The measurement points were on the forehead, the cheek, and on the petal of the nose. Results Sebum measurement showed some statistically significant differences between the sebum level on the cheek and the nose after a series of treatments. A higher hydration level was found at all measurement points. There were no statistically significant differences in TEWL parameter. pH measurement showed that the pH value decreased after each of the procedures. Most of the pigmentation measurements differed in a statistically significant way. Conclusion Results of the study indicate that the oxybrasion treatments increase the skin hydration and they have a significant influence on the decrease of the skin hydrolipid coat and pH.

Background Seed oil of sea buckthorn (SBT) is well known to contain high amount of polyunsaturated fatty acid (PUFA), and PUFA is generally acknowledged to promote skin hydration by reducing trans‐epidermal water loss (TEWL). Aims The present study is aimed to investigate that skin hydration offered by SBT seed oil is whether through up‐regulating AQP3 or HAS2 expression. Methods MTT assay was performed to detect cytotoxicity of SBT seed oil, and then, PCR was carried out to explore whether SBT seed oil can increase AQP3 mRNA expression in normal human epidermis keratinocytes (NHEK) cells or not. Immunofluorescence (IF) and Western blot analysis were used to test the protein level expression of AQP3 and HAS2 influenced by SBT seed oil in NHEK cells or in reconstructed epidermis skin model. Results According to the result of MTT assay, all test concentration of SBT seed oil showed no cytotoxicity to cells. 10 μg/mL SBT seed oil treatment evidently increased AQP3 mRNA level compared to negative control (NC). IF and Western blot analysis results demonstrated that AQP3 and HAS2 protein levels in NHEK cells treated with 10 μg/mL SBT seed oil were much higher than that of NC. Finally, treatment with 10 μg/mL SBT seed oil substantially up‐regulated expression of AQP3 and HAS2 protein in reconstructed epidermis skin model in comparison to NC. Conclusions In summary, our study first proved that SBT seed oil can improve skin hydration through increasing AQP3 and HAS2 expressions.

Background The efficacy of Djulis for skin care is currently based on cellular or animal models, and the clinical aspect is not in place. Aim This clinical study is to investigate the synergistic effect of fish collagen and Djulis (Chenopodium formosanum Koidz.) for improvement of skin parameters. We used the combination of hydrolyzed collagen and Djulis to develop a new functional formula for skin improvement. Patients/methods Fifty volunteers were randomly allocated (in a 1:1 ratio) to the placebo or collagen drink group. Volunteers were required to consume a 50 mL of a collagen drink or placebo drink daily for 8 weeks. For measurements, the indexes of skin conditions were measured at the baseline and 4 and 8 weeks. Results The improvements of skin hydration, brightness, crow's feet, texture, wrinkles, pores, spots, and collagen content after 8 weeks in collagen group were 17.8%, 5.4%, 14.9%, 9.9%, 29.3%, 10.4%, 9.9%, and 22.3%, respectively. Noticeably, over 68% of subjects got improved for their skin parameters after 8-week intake of collagen drink. The improvement levels indicated competitive skin improvement effects in comparison with previous studies. Conclusion This clinical study demonstrates the synergistic effect of fish collagen and Djulis (the main components) for the substantial improvements in hydration, brightness, crow's feet, texture, wrinkles, pores, surface spots, and collagen content in skin. The collagen drink comprehensively improved skin parameters for most subjects after 4-week intake and manifested competitive efficiency in comparison with other similar studies. We convince that the collagen drink may delay skin aging process and improve skin aging parameters.

Background Higher skin pH in atopic dermatitis contributes to impaired epidermal barrier. A moisturizer compatible with physiological pH could improve atopic dermatitis. Objective To determine the effect of a physiologically compatible pH moisturizer in atopic dermatitis. Methods A randomized half body, double blind, controlled trial involving patients with stable atopic dermatitis was performed. pH-modified moisturizer and standard moisturizer were applied to half body for 6 weeks. Results A total of 6 (16.7%) males and 30 (83.3%) females participated. Skin pH reductions from week 0, week 2 and 6 were significant at the forearms (5.315 [0.98] to 4.85 [0.54] to 5.04 [0.78], p = 0.02) and abdomen (5.25 [1.01], 4.82 [0.64], 5.01 [0.59], p = 0.00) but not at the shins (5.01 [0.80], 4.76 [0.49], 4.85 [0.79], p = 0.09) with pH-modified moisturizer. Transepidermal water loss (TEWL) at the forearms decreased (4.60 [2.55] to 3.70 [3.10] to 3.00 [3.55], p = 0.00), abdomen (3.90 [2.90] to 2.40 [3.45] to 2.70 [2.25], p = 0.046). SCORAD improved from 14.1 ± 12.75 to 10.5 ± 13.25 to 7 ± 12.25, p = 0.00. In standard moisturizer group, pH reductions were significant at the forearms (5.29 [0.94] to 4.84 [0.55] to 5.02 [0.70], p = 0.00) and abdomen (5.25 [1.09], 4.91 [0.63], 5.12 [0.66], p = 0.00). TEWL at the forearm were (4.80 [2.95], 4.10 [2.15], 4.60 [3.40], p = 0.67), shins (3.80 [1.40], 3.50 [2.35], 4.00 [2.50], p = 0.91) and abdomen (3.70 [2.45], 4.10 [3.60], 3.40 [2.95], p = 0.80). SCORAD improved from 14.2 ± 9.1 to 10.9 ± 10.65 to 10.5 ± 11, p = 0.00. Reduction in pH was observed with both moisturizers while TEWL significantly improved with pH-modified moisturizer. pH-modified moisturizer resulted in greater pH, TEWL and SCORAD improvements however the differences were not significant from standard moisturizer. Study limitation Skin hydration was not evaluated. Conclusion Moisturization is beneficial for atopic dermatitis; use of physiologically compatible pH moisturizer is promising.

Kakato-tsurutsuru (Kt) socks have been selling for almost 30 years in Japan. Wearers claim they improve heel dryness despite no scientific evidence. We investigated the effects of Kt socks on heel dryness by questionnaire, clinical scores and non-invasive skin measurements. In a double-blind, randomized cross-over study, 10 healthy volunteers wore control or Kt socks over 2 weeks in sequence for 4 weeks. Skin hydration and evaporation of the medial and dorsal heel were measured before and every week during the trial. Clinical evaluations of desquamation and cracked skin were scored by a dermatologist. A visual analog scale (VAS) questionnaire of comfort, sock climate humidity and skin dryness was conducted. The VAS of comfort was significantly higher in Kt than controls. Average Δskin dryness in control and Kt groups was -1.63 and 2.22, respectively, showing a significant improvement. In the clinical findings of the dorsal side of the heel, Δdesquamation and Δcracked skin scores were significantly decreased and Δstratum corneum hydration significantly increased in Kt compared with controls. Kt socks may retain evaporated sweat with components of natural moisturizing factors, supporting the water-holding ability of the heel stratum corneum. These findings suggest that Kt socks may improve heel skin dryness.

Salah satu permasalahan kesehatan lansia yang sering terjadi pada kulit adalah masalah kulit kering dan gatal. Gangguan ini mempengaruhi kualitas hidup lansia. Penelitian ini bertujuan untuk mengetahui perubahan kualitas hidup lansia secara holistik setelah intervensi peningkatan hidrasi kulit. Kualitas hidup secara holistik mencakup empat domain yaitu kesehatan fisik, psikologi, hubungan sosial, dan lingkungan. Penelitian dilakukan di Panti STW RIA Pembangunan Cibubur pada periode September 2019. Penelitian dilakukan secara quasi experimental, menggunakan kuesioner WHOQOL-BREF. Analisis statistik dilakukan menggunakan uji statistik Paired T-Test dengan uji alternatif Wilcoxon. Hasil menunjukkan terdapat 13 dari 26 butir pertanyaan pada WHOQOL-BREF yang mengalami perubahan yang signifikan karena intervensi yang dijalankan, yaitu pertanyaan Q1, Q2, Q10, Q15, Q16, Q5, Q6, Q11, Q19, Q26, Q23, dan Q24 (nilai p

Background Recently, many compounds or extracts from natural sources are used for reducing facial aging and wrinkles. Objective This study investigates the preparation and evaluation of the safety and efficacy of saffron extract and avocado oil used for anti-wrinkle topical cream. Methods and materials Oil in water cream was prepared containing avocado oil and the saffron extract. Accelerated stability tests were carried out due to 6 months accordingly through the International Council for Harmonization (ICH) guideline. For clinical studies, topical cream was applied by twenty healthy volunteers daily, for 12 weeks. Improvement in wrinkle grading by a Global Aesthetic Improvement Scale (GIAS), depth and volume of the nasolabial fold, thickness, and density of dermis, and determination of the skin biophysical parameters were evaluated at the baseline, 6th and 12th week. Result During the period of storage, the formulation showed no variation in physicochemical properties. The clinical evaluation showed at least one-grade improvement in GIAS in 30% and 45% of participants, after 6 and 12 weeks, respectively. Subjects were "moderately satisfied" with the treatment. In addition, the area and volume of nasolabial folds were significantly reduced. Moreover, net elasticity (R5) and gross elasticity (R2) showed a significant increase after 12 weeks of treatment. There was no report of allergic reactions, and no changes in skin hydration occurred during the treatment. Conclusion The results of this study showed that saffron extract and avocado oil anti-wrinkle topical cream is an efficient and safe treatment for facial skin rejuvenation.

BACKGROUND Epidermis hydration measurement is an important tool to assess its health. SkinUp® is a portable device that measures moisture and oil levels of skin through impedance method, it is cheap and has small dimensions, but this equipment has not yet been cited in the scientific literature. Thus, the objective of this work was to validate SkinUp® equipment by comparing its results with Corneometer® CM825. MATERIALS AND METHODS Twenty subjects had skin hydration measurement on forearm, cheeks, and forehead. Measurements obtained with the two instruments were correlated by calculating the Pearson correlation coefficient and the P-value significance. The sensitivity of the equipment to different regions and treatments and their accuracy were also evaluated. RESULTS Positive correlations between equipment were found for skin hydration, with P ≤ .001. Both instruments presented high sensitivity to the different treatments (P

Background Both intrinsic and environmental factors play a role in premature ageing of the skin. Natural extracts have been extensively used to reduce the signs of ageing in recent years. Pterostilbene is a natural analog of resveratrol with therapeutic properties against numerous diseases mainly due to its ability to reduce reactive oxygen species. Methods We studied the anti-aging and skin brightening effect of a 0.4% formulation of natural pterostilbene in healthy volunteers (N=38) in an open-label, single-arm, monocentric study for 8 weeks. The melanogenesis inhibitory, anti-tyrosinase and anti-collagenase and anti-elastase properties of pterostilbene were evaluated in vitro. Results Natural pterostilbene showed significant inhibition of melanogenesis in vitro. Pterostilbene cream (0.4%) was highly effective in reducing markers of aging and induces even skin tone. The product was effective in reducing wrinkles and fine lines, improved skin hydration elasticity and showed no adverse effects. Conclusion Our results suggest that natural pterostilbene is a safe and effective ingredient for use in cosmetic preparation to reduce the markers of skin ageing and brighten the skin tone. Trial registration Clinical Trial Registration India Identifier CTRI/2019/01/017160 [Registered on 18/01/2019].

Aim: To describe the methodology in studying patient’s acceptability and efficacy of an ectoin containing emollient for atopic dermatitis (AD). Methods: We described the methodology that we used in studying emollients and moisturisers, and patient acceptability of a group of AD patients before and following usage of an ectoin-containing proprietary emollient. These data were also compared with other brand emollients that we previously reported, namely Restoradom®, Ezerra® and Ezerra plus®. Results: 30 subjects (50% Male, Mean (SD) age: 9.8 (3.6) years with AD used the trial emollient W for four weeks. AD severity of subjects (by objective SCORAD) was moderate (n=22) and severe (n=8). Compliance was good and patients generally managed to use the moisturisers daily, with individual reports of a ‘tingly’ sensation by some subjects when applied to inflamed wounds. 63% reported “very good” or “good”, whereas 37% reported “fair” or “poor” acceptability of the moisturisers. Following use of the trial emollient, area affected, disease intensity and severity significantly improved, as demonstrated in objective SCORAD (p=0.002). There were also significant improvements in POEM (p=0.035), and PADQLQ scores (p=0.017). For skin measurements, only transepidermal water loss had improved (p=0.035) after the treatment. There was no significant improvement of itch or sleep scores, skin hydration, pH, S. aureus colonization status, or need for use of topical medications. When compared with historical data of other emollients, the mean age of patients on emollient W was younger; efficacy and acceptability among these emollients were similar. Conclusions: Methodology of emollient research is described. Doctors should provide evidencebased information about the efficacy of emollients. The ectoin-containing proprietary emollient improves disease and quality of life following its use in 4 weeks. Efficacy and acceptability are similar among 4 proprietary emollients.

The article presents modern data on the features of the structure of the skin and the formation of the epidermal barrier in premature and young children. Information is given regarding changes in the properties of the skin: microrelief, hydration, transepidermal water loss, acidity, content of the natural moisturizing factor, and sebum secretion. Attention is also paid to the role of vernix caseosa in the formation of a mature epidermal barrier and data on the use of emollients are provided.

Moringa oleifera seed oil has been recognized for its benefits in relation to the skin. The objective of this study was to evaluate the chemical composition and antioxidant activity of moringa seed oil, to formulate a moringa seed oil cream, and to determine the efficacy of moringa seed oil cream in vivo. The chemical components of moringa seed oil were analyzed by high-performance liquid chromatography and gas chromatography. The antioxidant activity of the oil was determined by a 2,2-diphenyl-1-picrylhydrazyl (DPPH) free-radical scavenging assay. An oil-in-water cream containing moringa seed oil was developed and characterized for antioxidant activity. The moringa seed oil cream was further subjected to the accelerated stability test of heating&ndash, cooling cycles for six cycles and stored isothermally at 4, 30, and 45 &deg, C for 28 days. The efficacy of moringa seed oil cream was investigated in 32 participants by measuring their skin hydration, erythema, melanin values, and visco-elasticity. The results showed that moringa seed oil contained &alpha, tocopherol, plant sterols, and fatty acids. The oil had antioxidant activity with a 50% of initial concentration (IC50) value of 121.9 mg/mL. The stability study indicated that the pH, viscosity, and rheological behavior of the cream containing moringa seed oil were not significantly changed after storage at 4, 30, and 45 &deg, C for 28 days and six heating&ndash, cooling cycles. The moringa seed oil cream exhibited in vitro antioxidant activity and increased the in vivo skin hydration level compared with the cream base. There was no report of skin irritation from moringa seed oil cream application, suggesting that the moringa seed oil cream developed in this study was appropriate for pharmaceutical and cosmetic uses. &nbsp, A M. oleifera seed oil cream was successfully developed. The moringa seed oil cream possessed antioxidant activity, enhanced the skin hydration level, and reduced skin erythema, but did not affect the melanin content and skin visco-elasticity. The moringa seed oil cream did not induce skin irritation and, thus, was safe to use.

Continues wearing gloves is a crucial problem among health care workers treating patients with coronavirus (COVID-19) infection that may cause skin complications. In the present study, the skin conductance (SC), skin potential (SP) and skin susceptance (SS) were used to identify the effects of long-term wearing gloves among health care workers managing COVID-19. The experimental procedure was repeated 30 times on three subjects recruited as volunteers to wear gloves for 4 hours. The baseline of parameters SC, SP, and SS were recorded at normal conditions without wearing the glove at zero time (To). The parameters were again recorded in the cases of glove-wearing with and without zinc oxide ointment (ZnO) for the same period. Noticeable differences were found in each of SC, SP and SS for subjects who wore gloves when compared to the baseline for 4 h. Results also showed significant differences in each of SP and SS during the same period with applying ZnO comparing to wearing gloves without applying ZnO. The results show that skin hydration is significantly improved by using ZnO. However, changing gloves at least every four hours of continuous wearing is recommended.

Identifying materials contributing to skin hydration, essential for normal skin homeostasis, has recently gained increased research interest. In this study, we investigated the potential benefits and mechanisms of action of Aspergillus oryzae-fermented wheat peptone (AFWP) on the proliferation and hydration of human skin keratinocytes, through in vitro experiments using HaCaT cell lines. The findings revealed that compared to unfermented wheat peptone, AFWP exhibited an improved amino acid composition, significantly (p &lt, 0.05) higher DPPH scavenging capability and cell proliferation activity, and reduced lipopolysaccharide-induced NO production in RAW 264.7 cells. Furthermore, we separated AFWP into eleven fractions, each ≤2 kDa, of these, fraction 4 (AFW4) demonstrated the highest efficacy in the cell proliferation assay and was found to be the key component responsible for the cell proliferation potential and antioxidant properties of AFWP. Additionally, AFW4 increased the expression of genes encoding natural moisturizing factors, including filaggrin, transglutaminase-1, and hyaluronic acid synthase 1–3. Furthermore, AFW4 activated p44/42 MAPK, but not JNK and p38 MAPK, whereas PD98059, a p44/42 MAPK inhibitor, attenuated the beneficial effects of AFW4 on the skin, suggesting that the effects of AFW4 are mediated via p44/42 MAPK activation. Finally, in clinical studies, AFW4 treatment resulted in increased skin hydration and reduced trans-epidermal water loss compared with a placebo group. Collectively, these data provide evidence that AFW4 could be used as a potential therapeutic agent to improve skin barrier damage induced by external stresses.

Daily use of cosmetics is known to affect the skin microbiome. This study aimed to determine the bacterial community structure and skin biophysical parameters following the daily application of a skincare product on the face. Twenty‐five Korean women, who used the same skincare product for four weeks participated in the study. During this period, skin hydration, texture, sebum content, and pH were measured, and skin swab samples were collected on the cheeks. The microbiota was analyzed using the MiSeq system. Through these experiments, bacterial diversity in facial skin increased and the microbial community changed after four weeks of skincare product application. The relative abundance of Cutibacterium and Staphylococcus increased, significant changes in specific bacterial modules of the skin microbial network were observed, and skin hydration and texture improved. It was suggested that daily use of skincare products could affect the microbial structure of facial skin as well as the biophysical properties of the facial skin. These findings expand our understanding of the role of skincare products on the skin environment., The daily use of skincare products affects both the facial skin microbial structure and its biophysical properties. Based on the canonical correspondence analysis plot, after the use of a skincare product, the skin microbiomes were significantly separated from the initial skin microbiome. Moreover, the study showed a significant correlation between skin microbial community and skin biophysical parameters such as skin hydration and texture, suggesting a relationship between these factors.

Background Nowadays, face masks are a crucial part of our daily life. Previous studies on their impact on the skin usually focused on the adverse effects of face masks. Few studies have assessed their influence on skin characteristics. In a previous study, we identified the short‐term effects of wearing face masks. Herein, we describe the long‐term skin effects of face masks, for a period of 6 months. Materials and methods Healthy volunteers (19 men and women), who wore face masks, participated in the study from June 2020 to December 2020. In all participants, skin characteristics such as trans‐epidermal water loss (TEWL), skin hydration, skin elasticity, skin pore area, skin keratin amount, skin temperature, skin redness, skin temperature, skin redness, and skin color were measured three times. Results TEWL, skin hydration, skin elasticity, skin pore area, skin keratin amount, and skin color changed significantly after 6 months. TEWL, skin hydration, skin pore area, skin keratin amount, and skin color were significantly different between the mask‐wearing and non‐mask‐wearing areas. Conclusion Long‐term daily use of face masks can alter skin characteristics. Special care should be focused on the mask‐wearing regions.

Manpreet Randhawa,1 Thomas Meyer,2 Mukta Sachdev,3 Ratan K Chaudhuri1 1Sytheon Ltd, Boonton, NJ, USA; 2Meyer Sun & Skin Care Consulting, LLC, Memphis, TN, USA; 3MSCR Pvt Ltd, Bengaluru, IndiaCorrespondence: Manpreet RandhawaSytheon Ltd, 315 Wootton Street, Boonton, NJ, USATel +1 973 947 1109Email manpreet@sytheonltd.comBackground: Identification of long-lasting natural antioxidants to protect against and repair skin damage induced by exposure to environmental pollution is in high demand.Objective: To investigate a standardized Terminalia chebula (TC) fruit extract for its long-lasting antioxidant and anti-inflammatory properties and its ability to reverse the visible signs of pollution-induced skin damage in an 8-week clinical study.Material and Methods: Chemical and cell-based in vitro studies were performed to characterize long-lasting antioxidant and anti-inflammatory properties; a clinical study with subjects with normal to dry skin living in a high-pollution city for the previous 5 years was conducted to assess if a formulation containing 1% standardized TC fruit extract affected significant improvements in skin’s visible condition and appearance compared with its placebo.Results: The standardized TC fruit extract provided longer-lasting and more efficient neutralization of reactive oxygen species (ROS) than tocopherol; treatment of keratinocytes with the fruit extract prior to being stressed with urban dust safeguarded against increases in intracellular ROS, inhibited release of inflammatory cytokines IL-6 and IL-8 and protected membrane lipids against peroxidation. A clinical study yielded statistically significant improvements in dermatologist scores and subject self-assessments for skin texture, hydration, tone, firmness and radiance as compared to its placebo.Conclusion: These studies validate the use of this standardized TC fruit extract not only as a restorative to diminish visible signs of existing damage but also as a preventative to help defend skin against damages caused by chronic exposure to environmental pollution.Keywords: long-lasting antioxidant, pollution, skin hydration, skin radiance, Terminalia chebula

Senile pruritus (SP) is a common skin disease in the elderly. The role of skin dysbacteriosis in the development of various skin diseases has been studied in recent years. However, the research about the skin microbiota of senile pruritus patients is lacking at present. The purpose of this cross-sectional study was to investigate the differences of skin microbiota in senile pruritus patients and their relationship with the epidermal barrier. Thirty patients with senile pruritus and 30 age- and sex-matched healthy controls were enrolled in this study. The skin barrier indexes were recorded by multi-functional skin tester. The skin bacterial diversity was analyzed by using hyper-variable tag sequencing of the V3-V4 region of the 16S rDNA. Compared with the healthy control group, the patients had significantly lower skin hydration (p = 0.014) and higher pH value (p = 0.021). Skin microbial diversity was significantly increased in patients according to the alpha diversity. At the genus level, Acinetobacter (p = 0.002) and Lactobacillus (p = 0.002) increased and Cutibacterium (p = 0.043) decreased. The pH value was positively associated with observed_species diversity (p = 0.026). The transdermal water loss was negatively related to the genus of Lactobacillus (p = 0.036), while the skin hydration was positively associated with the genus of Lactobacillus (p = 0.038). As a result, the damaged skin barrier function and skin dysbacteriosis complemented each other and may be associated with the occurrence of senile pruritus, but their role still needs further study.

The aim of this study was to estimate the consumption of selected dietary components with antioxidant properties, undertake body composition analysis, assess skin hydration and lubrication, and establish the relationships between the above parameters. The study was carried out on 172 young women. The consumption of ingredients (vitamins A, C, D and E, and Cu, Mn, Zn) was assessed using the Diet 6.0 program, body composition was assessed using electrical bioimpedance and skin hydration and lubrication were assessed using the corneometric and sebumetric methods, respectively. About one-third of students showed insufficient consumption of vitamin C, vitamin E and zinc, while about 99% showed insufficient vitamin D levels. The highest degree of hydration was observed in the areas of the eyelids, neckline and chin. The greatest amount of sebum was found in the area of the nose and forehead. Low positive correlations between hydration or lubrication and Cu, vitamin A and vitamin E were observed. In conclusion, to properly moisturize and lubricate the skin, young women should eat products that are rich in ingredients with antioxidant properties, in particular fat-soluble vitamins A and E, but also copper.

Two novel body/face wash gels enriched with emollient ingredients (including dexpanthenol) were developed for the daily care of dry skin. Two similarly designed 2-week studies (N = 42 each) were conducted to assess the biophysical and cosmetic performance of each of the new wash gels in healthy adults with dry skin. Instrumental measurements quantified the effects of the wash gels on stratum corneum (SC) hydration and transepidermal water loss (TEWL) (with and without a previous sodium lauryl sulfate (SLS) challenge) after single and repeated usage. Following single and repeated applications of the face wash gel to facial skin, as well as to dry SLS-undamaged and SLS-damaged skin of the forearm, skin hydration significantly increased. Similarly, after single and repeated usage of the body wash gel to dry SLS-undamaged and SLS-damaged skin of the forearm, skin moisturization increased significantly from baseline, comparisons with control areas provided inconsistent results for SLS-undamaged skin. No effects on TEWL were observed for either product. Both wash gels were well tolerated and the cosmetic performances were appreciated by the subjects. The study results suggest that daily use of the new wash gels was associated with significant skin-moisturizing effects without adversely affecting skin barrier function and repair.

Introduction Recently, there are a few moisturizers showing hydrating effects up to 24 hours after single application. Aquaporin 3 might be associated with the degree of skin hydration. We aimed to assess the effects of two brands of 24‐hour moisturizers on the skin barrier function, as well as the AQP3 gene expression. Method Two moisturizers were applied once daily by 20 participants age 36.15 ± 9.55 years. Upper right and left forearms were randomly assigned to application of each product, whereas the right lower forearm served as control site for application of a cream base formulation. Biophysical assessments including trans epidermal water loss (TEWL), skin hydration, pH, surface lipids, and elasticity parameters were performed before intervention, 1, 4, and 24 hours after single application, following 2 weeks daily application and 1 week after termination of use. Also 5‐mm punch biopsies were performed from application sites of product B and cream base formulation in for five participants after 2 weeks of application. Results A single treatment with both products led to 24‐hour increase in skin moisture in comparison with the control site (P‐value

Background Previous studies have demonstrated increased pore size and darkening skin color with total sleep deprivation. There are many studies of skin characteristics with short-term sleep restriction, but there are few studies on skin characteristics when sleep is restricted more than three consecutive days. This study evaluated skin changes with sleep limited to 4 hours per night for six nights. Materials and methods The study included 32 Korean women in their 40s. Skin hydration, desquamation, barrier recovery, texture, gloss, transparency, elasticity, crow's feet, frown lines, and color were measured. Individual sleep time was monitored by smartwatches. Subjects slept 8 hours per night for six nights in week one and 4 hours per night for six nights in week two. Results Skin hydration was significantly reduced after 1 day of sleep deprivation, and it continued to decrease. Skin gloss, desquamation, transparency, elasticity, and wrinkles were significantly aggravated after 1 day of sleep deprivation. Skin texture was significantly aggravated on the fourth day of sleep restriction. Elasticity was most affected by reduced sleep, with a standardized coefficient of -.320, indicating a significant decrease over time as compared to other characteristics. Conclusion Skin hydration was gradually decreased with sleep restriction. Skin texture did not change after only 1 day of sleep restriction. It is a new finding that elasticity decreases more than other skin characteristics with prolonged sleep restriction.

Background Subjective facial skin type is most frequently determined by the amount of sebum, which showed trends across subjective skin types in most previous studies while not in some. This study was conducted to evaluate the associations among subjective skin type, amount of sebum, stratum corneum hydration, and pore size in Thai women. Methods Sixty-two healthy women with either self-described subjective oily or dry skin type were included and casual sebum level (CSL), sebum excretion rate (SER), clinical pore size score, mean pore area, and stratum corneum hydration were measured at several facial sites. Correlation coefficients between amount of sebum and other parameters were estimated. Results Casual sebum level and sebum excretion rate were significantly higher in oily-skinned than dry-skinned group by 1.6-2.1 times. Mean pore area and clinical pore size score were not different between the 2 groups, nor did they correlate with CSL or SER. Corneometry was shown to be significantly higher in dry-skinned than oily-skinned group. Significant, negative correlations between corneometry and CSL were also found. Conclusion The subjective facial skin types were consistent with the amount of sebum, but not pore size or corneometry, among Thai women.

Background Hydration and moisturization both impact skin quality, directly reflecting its appearance. Signs and onset of dehydration-related skin aging are region-specific and require tailored treatment to be effective. Aims To test the hydrating effects of formulas containing a novel 3-dimensional 3-polymer interpenetrating network (3D3P-IPN) to deliver humectants and actives to specific body sites. Methods Two clinical studies were conducted focused on the skin under eyes and body (arms/legs). Healthy women ages 25-65 (eyes) or 35-65 (body) with mild to moderate dry and aged skin were enrolled. Study product containing the 3D3P-IPN and tailored actives was applied twice daily for 8 weeks on the periorbital area and for 4 weeks on the body. Changes in skin attributes were measured by biophysical instrumentation for hydration, dark circles, skin color, elasticity and transepidermal water loss, and by clinical grading and subject self-assessment. Results Significant improvements in hydration and skin smoothing were demonstrated in both studies. In the periorbital region, actives and humectants delivered by the 3D3P-IPN also led to significant improvements in dark circles, fine lines/crow's feet, puffiness, restoring radiance, and overall younger-looking appearance. On the arms and legs, there were significant reductions in crepiness and dullness. The arms and legs also had improvements in tactile and visual skin texture, radiance, and general healthy look. Improvements were immediate and persisted through the end of both studies. Conclusion The 3D3P-IPN provides immediate and long-lasting improvements in skin hydration and overall healthy appearance regardless of the targeted application site.

Dry skin is one of the most important concerns of consumers worldwide. Despite huge efforts over several decades, the personal care industry still does not offer a perfect solution to satisfy the unmet needs of consumers for moisturising treatments in different ethnic groups. The paucity of data for the underlying cellular and biochemical problems in, and the effects of moisturisers on photodamaged facial skin may partly explain this. Mainly, single point measurements are used to understand the effects of products on skin physiology even on surrogate skin sites such as the non‐photodamaged volar forearm. Some groups have developed discontinuous facial maps of skin biophysical properties, however, in 2014 a continuous facial analysis of bio‐instrumental evaluations was developed using a heat map approach. These maps enabled a continuous visualization of features that not only revealed an unexpected complexity of facial skin but also indicated that use of surrogate skin sites for facial skin is inappropriate. We have demonstrated that remarkable gradients of skin hydration, TEWL, skin surface pH and sebum exist within short distances across the face and the gradients are distinctive among different ethnic groups. In addition, these studies have demonstrated that darkly‐pigmented individuals do not necessarily have a better skin barrier function than their less‐pigmented counterparts and that Caucasians have a lower facial skin surface pH compared with more pigmented subjects. Overall, there are no correlations between capacitance, TEWL and skin surface pH including individual topology angle values. Novel 3D camera approaches have also been used to facilitate a more precise assignment of measurement sites and visualisation. The 3D facial colour mappings illustrated precisely the local moisturising effects of a moisturising cream. There were subtle ethnic differences in efficacy that may be related to underlying skin biochemistry and/or ethnic differences in product application. A placebo‐controlled study using conductance measurements in Chinese subjects is also reported. Finally, a new whole face statistical approach has been taken to prove differences in skin parameters but also of moisturiser treatment that adds further to our understanding of the ethnic differences in skin physiology and product application. This paper reviews the background of the development and application of this methodology., The skin mapping technology enables the transformation of bio‐instrumental data to continuous 2D and 3D visualisation of facial skin features. This approach reveals the complexity of facial skin and the differences of differently pigmented skin types.

Objective This study compared an antiaging treatment with two currently marketed cosmetic antiaging products for the treatment of lateral canthal lines ("crow's feet"). Methods Healthy female volunteers (72) aged of 54.6 years (mean) having fine‐to‐moderate wrinkles in the lateral canthal areas were randomized to one of three treatments applied daily over 28 days: Group A (Purgenesis™ Day Cream, Purgenesis™ Eye Cream, and Purgenesis™ Night Cream); Group B (Prevage® Eye Lotion, Prevage® Day Cream, and Prevage® Night Cream); or Group C (La Mer® Eye Balm, Crème de La Mer®, and La Mer® Night Cream). The effects on anti‐wrinkle properties and for sensory attributes and general performance were evaluated on Days 1, 7, and 28. Results Skin hydration improved significantly at all time points in Groups A and B, and at Day 28 in Group C. Group A patients experienced significant improvements in measured skin elasticity parameters at Day 28; extensibility and maximum amplitude were significantly better at Day 28 in Groups B and C. Benefits were also seen in profilometric parameters with statistical significance only in Group A Volunteer tolerance was good with all three treatments, although moderate and high levels of adverse events were numerically higher in Group B than in Groups A or C, and levels of slight discomfort were significantly more prevalent in Group B. Conclusion The Purgenesis™ antiaging treatment significantly improved skin hydration, elasticity, and profilometry parameters during a 28‐day study. This therapy was found to be well tolerated and effective in countering the cutaneous signs of aging.