1. Anticancer Efficacy and Mechanism of Amentoflavone for Sensitizing Oral Squamous Cell Carcinoma to Cisplatin
- Author
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Zhao Lin Tan, Yuan Hao Lee, Fei Ting Hsu, Chia Jung Tsai, Yi Chi Huang, Ta Chih Liu, Cheng Hsien Chen, Yao Chen Chuang, and Hsi Feng Tu
- Subjects
Cancer Research ,Antineoplastic Agents ,Apoptosis ,Amentoflavone ,Flow cytometry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Gentamicin protection assay ,Cell Movement ,Cell Line, Tumor ,medicine ,Biflavonoids ,Humans ,Neoplasm Invasiveness ,Viability assay ,Cytotoxicity ,Cisplatin ,medicine.diagnostic_test ,Chemistry ,NF-kappa B ,NF-κB ,General Medicine ,stomatognathic diseases ,Treatment Outcome ,Oncology ,Caspases ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Cancer research ,Mouth Neoplasms ,medicine.drug - Abstract
Background/aim Nuclear factor kappa B (NF-κB) inactivation and apoptosis activation have been shown to enhance the anticancer effect of cisplatin in oral squamous cell carcinoma (OSCC). Amentoflavone may suppress NF-κB activity and trigger apoptosis in different types of cancer. The aim of this study was to investigate the anticancer effect and mechanism of amentoflavone in combination with cisplatin in OSCC. Materials and methods We investigated the combination effect and mechanism of amentoflavone and cisplatin via cell viability analysis, flow cytometry-based apoptosis analyses, transwell migration/invasion assay, immunofluorescence staining and western blotting assay. Results Both amentoflavone and QNZ (NF-κB inhibitor) significantly increased cisplatin-induced cytotoxicity. Amentoflavone reduced cisplatin-triggered NF-κB activity and enhanced cisplatin-induced intrinsic caspase-dependent and independent apoptotic pathways. Moreover, amentoflavone augments cisplatin-suppressed invasion and migration ability of OSCC cells. Conclusion Inactivation of NF-κB and induction of apoptosis through intrinsic caspase-dependent and independent apoptotic pathways are associated with amentoflavone enhanced anti-OSCC efficacy of cisplatin.
- Published
- 2020