Effect of berberine on activity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549

ObjectiveTo study the effects of berberine on activity and mRNA expression of N-acetyltransferase in human lung cancer cell line A549.MethodsN-acetyltransferase antibodies were prepared. The human lung cancer A549 cells were cultivated randomly in the wells of culture plate, and divided into the control group, and berberine 0.0008, 0.008, 0.08, 0.8 and 1.6 mM treatment groups, with 3 wells for each group. 24 h later, A549 cells in each group were collected respectively, the content of N-acetyltransferase was detected by Flow cytometry, and the mRNA expression of N-acetyltransferase was observed by reverse transcription polymerase chain reaction.ResultsThe N-acetyltransferase content in human lung cancer A549 cells decreased with the increasing of berberine concentration, significantly lower than that in the control group (P