51 results on '"Xiao-Mei Hu"'
Search Results
2. Performance Assessment of Internal Quality Control (IQC) Products in Blood Transfusion Compatibility Testing in China.
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Gui-Ping Xu, Li-Fang Wu, Jing-Jing Li, Qi Gao, Zhi-Dong Liu, Qiong-Hua Kang, Yi-Jun Hou, Luo-Chuan Zhang, Xiao-Mei Hu, Jie Li, and Juan Zhang
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Medicine ,Science - Abstract
Internal quality control (IQC) is a critical component of laboratory quality management, and IQC products can determine the reliability of testing results. In China, given the fact that most blood transfusion compatibility laboratories do not employ IQC products or do so minimally, there is a lack of uniform and standardized IQC methods. To explore the reliability of IQC products and methods, we studied 697 results from IQC samples in our laboratory from 2012 to 2014. The results showed that the sensitivity and specificity of the IQCs in anti-B testing were 100% and 99.7%, respectively. The sensitivity and specificity of the IQCs in forward blood typing, anti-A testing, irregular antibody screening, and cross-matching were all 100%. The reliability analysis indicated that 97% of anti-B testing results were at a 99% confidence level, and 99.9% of forward blood typing, anti-A testing, irregular antibody screening, and cross-matching results were at a 99% confidence level. Therefore, our IQC products and methods are highly sensitive, specific, and reliable. Our study paves the way for the establishment of a uniform and standardized IQC method for pre-transfusion compatibility testing in China and other parts of the world.
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- 2015
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3. Genome-wide identification and expression profile analysis of citrus sucrose synthase genes: investigation of possible roles in the regulation of sugar accumulation.
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Mohammad Zahidul Islam, Xiao-Mei Hu, Long-Fei Jin, Yong-Zhong Liu, and Shu-Ang Peng
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Medicine ,Science - Abstract
Sucrose synthase (Sus) (EC 2.4.1.13) is a key enzyme for the sugar accumulation that is critical to form fruit quality. In this study, extensive data-mining and PCR amplification confirmed that there are at least six Sus genes (CitSus1-6) in the citrus genome. Gene structure and phylogeny analysis showed an evolutionary consistency with other plant species. The six Sus genes contain 12-15 exons and 11-14 introns and were evenly distributed into the three plant Sus groups (CitSus1 and CitSus2 in the Sus I group, CitSus3 and CitSus6 in the Sus II group, and CitSus4 and CitSus5 in the Sus III group). Transcripts of these six CitSus genes were subsequently examined. For tissues and organs, CitSus1 and 2 were predominantly expressed in fruit juice sacs (JS) whereas CitSus3 and 4 were predominantly expressed in early leaves (immature leaves), and CitSus5 and 6 were predominantly expressed in fruit JS and in mature leaves. During fruit development, CitSus5 transcript increased significantly and CitSus6 transcript decreased significantly in fruit JS. In the fruit segment membrane (SM), the transcript levels of CitSus2 and 5 were markedly higher and the abundant levels of CitSus3 and 6 gradually decreased. Moreover, transcript levels of CitSus1-4 examined were higher and the CitSus5 transcript level was lower in the fruit SM than in fruit JS, while CitSus6 had a similar transcript level in fruit JS and SM. In addition, transcripts of CitSus1-6 responded differently to dehydration in mature leaves or to mild drought stress in fruit JS and SM. Finally, the possible roles of Sus genes in the regulation of sugar accumulation are discussed; however, further study is required.
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- 2014
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4. Molecular epidemiological characterization and health burden of thalassemia in Jiangxi Province, P. R. China.
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Min Lin, Tian-Yu Zhong, Yi-Guo Chen, Jian-Zhong Wang, Jiao-Ren Wu, Fen Lin, Xin Tong, Hui-Tian Yang, Xiao-Mei Hu, Rong Hu, Xiao-Fen Zhan, Hui Yang, Zhao-Yun Luo, Wen-Yu Li, and Li-Ye Yang
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Medicine ,Science - Abstract
BACKGROUND: Thalassemia is the most common inherited disease in southern China. However, this disorder is usually ignored by Jiangxi provincial health system and government due to lack of epidemiological data. MATERIALS AND METHODS: A total of 9489 samples from Hakka Han and Gan-speaking Han in three geographical areas of Jiangxi Province were analyzed for both complete blood cell (CBC) count and reverse dot blot (RDB) gene chip for thalassemia. RESULTS: 1182 cases of suspected thalassemia carriers with microcytosis (MCV
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- 2014
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5. Digital gene expression analysis of corky split vein caused by boron deficiency in 'Newhall' Navel Orange (Citrus sinensis Osbeck) for selecting differentially expressed genes related to vascular hypertrophy.
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Cheng-Quan Yang, Yong-Zhong Liu, Ji-Cui An, Shuang Li, Long-Fei Jin, Gao-Feng Zhou, Qing-Jiang Wei, Hui-Qing Yan, Nan-Nan Wang, Li-Na Fu, Xiao Liu, Xiao-Mei Hu, Ting-Shuai Yan, and Shu-Ang Peng
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Medicine ,Science - Abstract
Corky split vein caused by boron (B) deficiency in 'Newhall' Navel Orange was studied in the present research. The boron-deficient citrus exhibited a symptom of corky split vein in mature leaves. Morphologic and anatomical surveys at four representative phases of corky split veins showed that the symptom was the result of vascular hypertrophy. Digital gene expression (DGE) analysis was performed based on the Illumina HiSeq™ 2000 platform, which was applied to analyze the gene expression profilings of corky split veins at four morphologic phases. Over 5.3 million clean reads per library were successfully mapped to the reference database and more than 22897 mapped genes per library were simultaneously obtained. Analysis of the differentially expressed genes (DEGs) revealed that the expressions of genes associated with cytokinin signal transduction, cell division, vascular development, lignin biosynthesis and photosynthesis in corky split veins were all affected. The expressions of WOL and ARR12 involved in the cytokinin signal transduction pathway were up-regulated at 1(st) phase of corky split vein development. Furthermore, the expressions of some cell cycle genes, CYCs and CDKB, and vascular development genes, WOX4 and VND7, were up-regulated at the following 2(nd) and 3(rd) phases. These findings indicated that the cytokinin signal transduction pathway may play a role in initiating symptom observed in our study.
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- 2013
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6. Comparison of the effects between MPL and JAK2V617F on thrombosis and peripheral blood cell counts in patients with essential thrombocythemia: a meta-analysis
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Jing Ming, Richeng Quan, Yujin Li, Mingjing Wang, Ziqing Wang, Xueying Wang, Haiyan Xiao, Erpeng Yang, Xiao-Mei Hu, and Weiyi Liu
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Blood cells ,medicine.medical_specialty ,MPL ,Mutation, Missense ,Essential thrombocythemia ,Cochrane Library ,Gastroenterology ,Risk Factors ,White blood cell ,Internal medicine ,medicine ,Humans ,Peripheral blood cell ,Platelet ,Prospective Studies ,JAK2V617F ,Retrospective Studies ,Hematology ,business.industry ,Thrombosis ,General Medicine ,Janus Kinase 2 ,medicine.disease ,Blood Cell Count ,Meta-analysis ,medicine.anatomical_structure ,Mutation ,Original Article ,business ,Receptors, Thrombopoietin ,Thrombocythemia, Essential - Abstract
To assess the effects between MPL and JAK2V617F on the thrombosis risk and peripheral blood cell counts in patients with essential thrombocythemia (ET), we identified eligible studies from PubMed, Embase, and the Cochrane Library. Seven studies were ultimately included in this meta-analysis. All studies reported the peripheral blood cell counts of ET patients, and three of them reported the eligible thrombotic events. In comparing the effect of MPL versus JAK2V617F on thrombosis, 1257 ET patients (73 MPL + and 1184 JAK2V617F +) were included. MPL-positive (MPL +) ET patients had a higher risk of thrombosis than JAK2V617F-positive (JAK2V617F +) ET patients [RR = 1.80 (1.08–3.01), P = 0.025]. And 3453 ET patients (138 MPL + and 3315 JAK2V617F +) were included in the comparison of peripheral blood cell counts. Platelet counts of MPL + ET patients were higher than that of JAK2V617F + ET patients [WMD = 81.18 (31.77–130.60), P = 0.001]. MPL + ET patients had lower hemoglobin [WMD = − 11.66 (− 14.32 to − 9.00), P = 0.000] and white blood cell counts [WMD = − 1.01 (− 1.47 to − 0.56), P = 0.000] than JAK2V617F + ET patients. These findings indicate that the MPL mutation is a high-risk factor for thrombosis in ET patients, and it may be rational to include MPL mutation in the revised IPSET as a criterion for thrombosis prediction scores. And given the differences in peripheral blood, it is necessary to further study whether MPL + ET patients differ from JAK2V617F + ET patients in bleeding and survival.
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- 2021
7. Novel deletion mutation in Bruton’s tyrosine kinase results in X-linked agammaglobulinemia: A case report
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Chun Chen, Chun-Lin Wang, Hong Chen, Xiao-Mei Hu, Ke Yuan, Yanlan Fang, Jianfang Zhu, and Li Liang
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Genetics ,biology ,business.industry ,X-linked agammaglobulinemia ,General Medicine ,medicine.disease ,03 medical and health sciences ,0302 clinical medicine ,Agammaglobulinemia ,Bruton’s tyrosine kinase ,hemic and lymphatic diseases ,030220 oncology & carcinogenesis ,Deletion mutation ,Mutation ,Case report ,Mutation (genetic algorithm) ,biology.protein ,medicine ,Immunodeficiency ,Bruton's tyrosine kinase ,030211 gastroenterology & hepatology ,business ,Tyrosine kinase - Abstract
BACKGROUND X-linked agammaglobulinemia is a primary immunodeficiency disease caused by gene mutations of Bruton’s tyrosine kinase (BTK). We found a new mutation point and summarized the correlation analysis and performed a literature review. CASE SUMMARY The proband was a 5-year-old boy. He was admitted to our hospital due to a recurrent cough and a fever that had persisted for a month. He had a history of multiple respiratory infections and sinusitis. There was no immunodeficiency or recurrent infection history among his family members. Agammaglobulinemia was characterized as follows: Immunoglobulin (Ig) A, 90.0 mg/dL (90-450 mg/dL); IgG, 20.0 mg/dL (800-1800 mg/dL); and IgM, 18.0 mg/dL (60-280 mg/dL). Notably, the assessment of IgG subtypes revealed the following very low levels: Subtype 1, 0.26 g/L (3.62-12.28 g/L); subtype 2, 0.10 g/L (0.57-2.9 g/L); subtype 3, 0.009 g/L (0.129-0.789 g/L); and subtype 4, 0.003 g/L (0.013-1.446 g/L). Cellular immunological test results were as follows: CD3, 74.6% (50%-84.0%); CD4, 47.3% (27.0%-51.0%); and CD8, 24.9% (15.0%-44.0%). A de novo hemizygous deletion in BTK was detected: c.902_c.904delAAG/p.E301del. Transcript levels of the mutant BTK were similar to those of the wild-type gene, though overexpression resulted in markedly reduced levels of mutant BTK (9.49% ± 1.58%), relative to the wild-type BTK (75.8% ± 2.98%, P < 0.01). CONCLUSION This case of X-linked agammaglobulinemia was attributed to a de novo hemizygous deletion mutation in BTK (c.902_c.904delAAG/p.E301del). The mutation resulted in markedly reduced BTK protein stability in vitro.
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- 2020
8. Arsenic Disulfide Promoted Hypomethylation by Increasing DNA Methyltransferases Expression in Myelodysplastic Syndrome
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Qing-Bing Zhou, Yong-Gang Xu, Xiaoqing Guo, Zheng-Tang Liu, Hongzhi Wang, and Xiao-Mei Hu
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0301 basic medicine ,Pharmacology ,Methyltransferase ,DNMT3B ,Pharmaceutical Science ,Cancer ,Methylation ,Biology ,medicine.disease ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Drug Discovery ,DNA methylation ,Cancer research ,DNMT1 ,medicine ,Gene ,DNA hypomethylation - Abstract
Background Previous studies have shown that DNA methylation plays a significant role in myelodysplastic syndrome (MDS). In addition to hypermethylation, aberrant hypomethylation can result in the transcriptional activation of oncogenes in cancer, including MDS. Therefore, drugs targeting DNA hypomethylation are needed for the treatment of MDS. This study aimed to investigate whether As2S2 promoted hypomethylation by increasing DNA methyltransferases (DNMTs) expression in MDS. Patients and methods Ten bone marrow samples from MDS patients and 3 healthy donors were obtained for the examination of the DNA methylation with a Human Methylation 850K Bead Chip. The mRNA expressions for the DNMTs in the ten MDS patients and 3 controls were compared by Q-PCR. Then, the MDS cell line SKM-1 was treated with As2S2. After 2 days of treatment, Human Methylation 850K Bead Chip was applied to analyze the changes of gene methylation status in the cells. Q-PCR and Western blot were taken to test the changes of mRNA and protein expressions for DNMTs in SKM-1 cells after treatment. Results Five hundred ninety-two abnormally hypomethylated genes were found in MDS patients compared to those in controls by Human Methylation 850K. The mRNA expressions of DNMTs (DNMT1, DNMT3a and DNMT3b) in MDS patients were significantly lower than those in healthy individuals. The IC50 value of As2S2 for SKM-1 cells was 4.97 μmol/L.Treatment with As2S2 at 2 μmoL/L resulted in significant alterations in the methylation levels at 1718 sites in SKM-1 cells compared to those in the controls. Hypermethylation was observed in 1625 sites (94.58%), corresponding to 975 genes, compared to those in the controls. Finally, the expression levels of DNMTs (DNMT1, DNMT3a, and DNMT3b) significantly increased in SKM-1 cells treated with As2S2 at 2 μmoL/L and 4 μmoL/L. Conclusion These data show a potential clinical application of As2S2 as an innovative hypermethylation agent in MDS.
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- 2020
9. Ruxolitinib-based combinations in the treatment of myelofibrosis: worth looking forward to
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Weiyi Liu, Jing Ming, Shirong Zhu, Xueying Wang, Xiao-Mei Hu, and Yujin Li
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Oncology ,medicine.medical_specialty ,Ruxolitinib ,Pracinostat ,Myelofibrosis ,Review Article ,law.invention ,chemistry.chemical_compound ,Randomized controlled trial ,law ,Panobinostat ,Internal medicine ,Nitriles ,medicine ,Humans ,Immunologic Factors ,Adverse effect ,Clinical Trials as Topic ,business.industry ,Ruxolitinib-based combinations ,General Medicine ,Hematology ,medicine.disease ,Thalidomide ,Pyrimidines ,Treatment Outcome ,Tolerability ,chemistry ,Primary Myelofibrosis ,Pyrazoles ,Drug Therapy, Combination ,business ,medicine.drug - Abstract
Ruxolitinib is a targeted drug to treat myelofibrosis (MF). Ruxolitinib has significant advantages in spleen reduction and increasing 5-year overall survival (OS), and ruxolitinib-based combinations might provide more benefits than ruxolitinib monotherapy. In this review, we focus on the data of ruxolitinib-based combinations therapies and treatment-related adverse events (AEs) and safety. We analyzed and summarized the data of ruxolitinib-based combinations. Ruxolitinib combined with prednisone + thalidomide + danazol (TPD), panobinostat, pracinostat, azacytidine, or hydroxyurea has well reduced spleen. Ruxolitinib combined with danazol or TPD had well therapies in improvement of hemoglobin (Hgb) and platelets (PLT). Most ruxolitinib-based combinations therapies showed a superior benefit on reduced treatment-related AEs than ruxolitinib monotherapy. Treatment-related AEs and dose modification affect the safety and tolerability of ruxolitinib-based combinations. Genetic testing before treatment is recommended. To provide better clinical guidance, comparisons of these randomized controlled trials with the trials of ruxolitinib alone are necessary. This review suggests that the clinical application of ruxolitinib-based combinations is worth waiting for.
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- 2020
10. Arsenic-Containing Qinghuang Powder (青黄散) Is An Alternative Treatment for Elderly Acute Myeloid Leukemia Patients Refusing Low-Intensity Chemotherapy
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Chi Liu, Teng Fan, Yong-Gang Xu, Richeng Quan, Haiyan Xiao, Yan Lv, Xiao-Mei Hu, Xu-Dong Tang, Hongzhi Wang, Weiyi Liu, Liu Li, and Xiaoqing Guo
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Male ,medicine.medical_specialty ,medicine.medical_treatment ,0211 other engineering and technologies ,Antineoplastic Agents ,02 engineering and technology ,030226 pharmacology & pharmacy ,Arsenicals ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,021105 building & construction ,Humans ,Medicine ,Pharmacology (medical) ,Aged ,Retrospective Studies ,Aged, 80 and over ,Chemotherapy ,Performance status ,business.industry ,Significant difference ,Myeloid leukemia ,General Medicine ,Middle Aged ,Alternative treatment ,Leukemia, Myeloid, Acute ,Complementary and alternative medicine ,Bone marrow suppression ,Female ,Digestive tract ,Powders ,business ,Comorbidity index ,Drugs, Chinese Herbal - Abstract
To analyze the overall survival (OS) of elderly acute myeloid leukemia (AML) patients treated with oral arsenic-containing Qinghuang Powder (青黄散, QHP) or low-intensity chemotherapy (LIC). Forty-two elderly AML patients treated with intravenous or subcutaneous LIC (1 month for each course, at least 3 courses) or oral QHP (3 months for each course, at least 2 courses) were retrospectively analyzed from January 2015 to December 2017. The main endpoints of analysis were OS and 1-, 2-, 3-year OS rates of patients, respectively. And the adverse reactions induding bone marrow suppression, digestive tract discomfort and myocardia injury were observed. Out of 42 elderly AML patients, 22 received LIC treatment and 20 received QHP treatment, according to patients’ preference. There was no significant difference on OS between LIC and QHP patients (13.0 months vs. 13.5 months, >0.05). There was no significant difference on OS rates between LIC and QHP groups at 1 year (59.1% vs. 70.0%), 2 years (13.6% vs. 15%), and 3 years (4.6% vs. 5.0%, all >0.05). Furthermore, there was no significant difference of OS on prognosis stratification of performance status > 2 (12 months vs. 12 months), age> 75 year-old (12.0 months vs. 12.5 months), hematopoietic stem cell transplant comorbidity index >2 (12 months vs. 13 months), poor cytogenetics (12 months vs. 8 months), and diagnosis of secondary AML (10 months vs. 14 months) between LIC and QHP patients (>0.05). QHP may be an alternative treatment for elderly AML patients refusing LIC therapy.
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- 2019
11. Relation of Blood Arsenic Concentration with Effect and Safety of Arsenic-Containing Qinghuang Powder (青黄散) in Patients with Myelodysplastic Syndrome
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Yong-Gang Xu, Bowen Yi, Xiaoqing Guo, Xiao-Mei Hu, Qian-Zhe Zhu, Zhongyang Deng, Xiao-hong Shang, Hongzhi Wang, Rou Ma, Shirong Zhu, Mingjing Wang, Pan Zhao, and Su Fang
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medicine.medical_specialty ,Abdominal pain ,Blood transfusion ,Hemoglobin increased ,medicine.medical_treatment ,Karyotype ,0211 other engineering and technologies ,chemistry.chemical_element ,02 engineering and technology ,030226 pharmacology & pharmacy ,Gastroenterology ,Arsenicals ,Arsenic ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,021105 building & construction ,medicine ,Humans ,Blood Transfusion ,Pharmacology (medical) ,In patient ,business.industry ,General Medicine ,Blood Cell Count ,Diarrhea ,Complementary and alternative medicine ,chemistry ,Myelodysplastic Syndromes ,Digestive tract ,Powders ,medicine.symptom ,business ,After treatment ,Drugs, Chinese Herbal - Abstract
To investigate the relation of blood arsenic concentration (BAC) with clinical effect and safety of arsenic-containing Qinghuang Powder (青黄散, QHP) in patients with myelodysplastic syndrome (MDS). Totally 163 patients with MDS were orally treated with QHP for 2 courses of treatment, 3 months as 1 course. The BACs of patients were detected by atomic fluorescence spectrophotometry at 1, 3, and 6 months during the treatment, and the effective rate, hematological improvement and safety in patients after treatment with QHP were analyzed. After 2 courses of treatment, the total effective rate was 89.6% (146/163), with 31.3% (51/163) of hematological improvement and 58.3% (95/163) of stable disease. The hemoglobin increased from 73.48 ± 19.30 g/L to 80.39 ± 26.56 g/L (P 0.05). Among 46 patients previously depended on blood transfusion, 28.3% (13/46) completely got rid of blood transfusion and 21.7% (10/46) reduced the volume of blood transfusion by more than 50% after treatment. The BACs were significantly increased in patients treated for 1 month with 32.17 ± 18.04 μ g/L (P 0.05). The adverse reactions of digestive tract during the treatment were mild abdominal pain and diarrhea in 14 cases (8.6%), and no patients discontinued the treatment. The BACs of patients with gastrointestinal adverse reactions were significantly lower than those without gastrointestinal adverse reactions (22.39 ± 10.38 vs. 37.89 ± 11.84, μ g/L, P
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- 2019
12. Arsenic Dispensing Powder Promotes Erythropoiesis in Myelodysplastic Syndromes via Downregulation of HIF1A and Upregulation of GATA Factors
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Xiaomin Feng, Pan Zhao, Zhongyang Deng, Yuting Tang, Teng Fan, Wang Yue, Xiaomei Yan, Asumi Yokota, Rou Ma, Hongzhi Wang, Haiyan Xiao, Weiyi Liu, Gang Huang, Mingjing Wang, and Xiao-Mei Hu
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Genetically modified mouse ,Anemia ,Down-Regulation ,GATA Transcription Factors ,Arsenicals ,Cell Line ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Downregulation and upregulation ,hemic and lymphatic diseases ,Medicine ,Animals ,Humans ,Erythropoiesis ,030304 developmental biology ,Retrospective Studies ,0303 health sciences ,biology ,business.industry ,Myelodysplastic syndromes ,General Medicine ,medicine.disease ,Hypoxia-Inducible Factor 1, alpha Subunit ,Up-Regulation ,HIF1A ,Complementary and alternative medicine ,Cell culture ,030220 oncology & carcinogenesis ,Myelodysplastic Syndromes ,Cancer research ,biology.protein ,Mdm2 ,Powders ,business ,Drugs, Chinese Herbal - Abstract
Traditional Chinese Medicine (TCM) is a practical medicine based on thousands of years of medical practice in China. Arsenic dispensing powder (ADP) has been used as a treatment for MDS patients with a superior efficacy on anemia at Xiyuan Hospital of China Academy of Chinese Medical Sciences. In this study, we retrospectively analyzed MDS patients that received ADP treatment in the past 9 years and confirmed that ADP improves patients’ anemia and prolongs overall survival in intermediate-risk MDS patients. Then, we used the MDS transgenic mice model and cell line to explore the drug mechanism. In normal and MDS cells, ADP does not show cellular toxicity but promotes differentiation. In mouse MDS models, we observed that ADP showed significant efficacy on promoting erythropoiesis. In the BFU-E and CFU-E assays, ADP could promote erythropoiesis not only in normal clones but also in MDS clones. Mechanistically, we found that ADP could downregulate HIF1A in MDS clones through upregulation of VHL, P53 and MDM2, which is involved in two parallel pathways to downregulate HIF1A. We also confirmed that ADP upregulates GATA factors in normal clones. Thus, our clinical and experimental studies indicate that ADP is a promising drug to promote erythropoiesis in both MDS and normal clones with a superior outcome than current regular therapies. ADP promotes erythropoiesis in myelodysplastic syndromes via downregulation of HIF1A and upregulation of GATA factors.
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- 2021
13. Autophagy Gene Panel-Based Prognostic Model in Myelodysplastic Syndrome
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Ming-Jing Wang, Wei-Yi Liu, Xue-Ying Wang, Yu-Meng Li, Hai-Yan Xiao, Ri-Cheng Quan, Gang Huang, and Xiao-Mei Hu
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0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,autophagy ,autophagy-related genes ,lcsh:RC254-282 ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Gene panel ,Overall survival ,medicine ,prognostic model ,In patient ,Original Research ,Receiver operating characteristic ,business.industry ,Hazard ratio ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Training cohort ,Confidence interval ,myelodysplastic syndrome ,030104 developmental biology ,030220 oncology & carcinogenesis ,Prognostic model ,business - Abstract
Abnormal autophagy is related to the pathogenesis and clinical symptoms of myelodysplastic syndrome (MDS). However, the effect of autophagy-related genes (ARGs) on the prognosis of MDS remains unclear. Here, we examined the expression profile of 108 patients with MDS from the GSE58831 dataset, and identified 22 genes that were significantly associated with overall survival. Among them, seven ARGs were screened and APIs were calculated for all samples based on the expression of the seven ARGs, and then, MDS patients were categorized into high- and low-risk groups based on the median APIs. The overall survival of patients with high-risk scores based on these seven ARGs was shorter than patients with low-risk scores in both the training cohort (P = 2.851e-06) and the validation cohort (P = 9.265e-03). Additionally, API showed an independent prognostic indicator for survival in the training samples [hazard ratio (HR) = 1.322, 95% confidence interval (CI): 1.158–1.51; P < 0.001] and the validation cohort (HR = 1.05, 95% CI: 1–1.1; P < 0.01). The area under the receiver operating characteristic curve (AUROC) of API and IPSS were 43.0137 and 66.0274 in the training cohorts and the AUC of the validation cohorts were 41.5361 and 72.0219. Our data indicate these seven ARGs can predict prognosis in patients with MDS and could guide individualized treatment.
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- 2021
14. Cytogenetic characteristics of 665 patients with myelodysplastic syndrome in China: A single-center report
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Xueying Wang, Teng Fan, Chi Liu, Yumeng Li, Hongzhi Wang, Richeng Quan, Liu Li, Xiupeng Yang, Yong-Gang Xu, Shan-shan Zhang, Zhuo Chen, Xiao-Mei Hu, Weiyi Liu, Haiyan Xiao, Xiaoqing Guo, Yan Lv, Xudong Tang, Mingjing Wang, and Rou Ma
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0301 basic medicine ,Cancer Research ,medicine.medical_specialty ,Trisomy 8 ,Single Center ,Gastroenterology ,chromosome karyotype ,cytogenetics ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Medicine ,WHO classification ,prognostic scoring system ,business.industry ,Incidence (epidemiology) ,Cytogenetics ,Cancer ,Karyotype ,Articles ,medicine.disease ,myelodysplastic syndrome ,030104 developmental biology ,Oncology ,International Prognostic Scoring System ,Dysplasia ,030220 oncology & carcinogenesis ,business - Abstract
The karyotype is highly important for diagnosis and prognosis in myelodysplastic syndrome (MDS). The objective of the present study was to investigate the cytogenetic characteristics of patients with MDS in China. The karyotypes of 665 Chinese patients with MDS were analyzed, and it was identified that 298 cases (298/665, 44.8%) had abnormal karyotypes. Among the 298 patients with abnormal karyotypes, the 75 patients with trisomy 8 (+8) constituted the most common subset (75/298, 25.2%). The incidence of abnormal karyotypes was significantly higher in patients who were ≥51 years old compared with those
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- 2020
15. Prognostic value of ASXL1 mutations in patients with primary myelofibrosis and its relationship with clinical features: a meta-analysis
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Zi-Qing Wang, Xiao-Mei Hu, Yujin Li, Weiyi Liu, Xueying Wang, Mingjing Wang, Jing Ming, Erpeng Yang, and Richeng Quan
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Oncology ,Male ,medicine.medical_specialty ,Carcinogenesis ,Cochrane Library ,Disease-Free Survival ,Sex Factors ,Internal medicine ,medicine ,Humans ,Myelofibrosis ,Aged ,Acute leukemia ,Hematology ,Leukemia ,business.industry ,Hazard ratio ,Age Factors ,Clinical features ,General Medicine ,Random effects model ,medicine.disease ,Confidence interval ,Neoplasm Proteins ,ASXL1 mutations ,Repressor Proteins ,Survival Rate ,Meta-analysis ,Primary myelofibrosis ,Acute Disease ,Mutation ,Female ,Original Article ,business ,Prognostic value - Abstract
Additional sex combs like 1 (ASXL1) mutations are one of the most common molecular biological abnormalities in patients with primary myelofibrosis (PMF), and the effect of these mutations on prognosis remains controversial. Hence, we conducted a meta-analysis to assess the prognostic value and clinical characteristics of ASXL1 mutations in PMF patients. Eligible studies were systematically searched from PubMed, Embase, and the Cochrane Library. We extracted the hazard ratios (HRs) and their 95% confidence intervals (CIs) of overall survival (OS) and leukemia-free survival (LFS), the number of patients transformed to acute leukemia, and clinical characteristics to carry out a meta-analysis by fixed effect model or random effect model according to the heterogeneity between studies. A total of 4501 PMF patients from 16 cohorts of 14 studies were included in this meta-analysis. The results revealed that ASXL1 mutations might predict a shorter OS (HR = 2.30, 95% CI: 1.79–2.94, P P = 0.0003; the rate of acute leukemia transformation: OR = 2.06, 95% CI: 1.50–2.83, P
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- 2020
16. Network Pharmacology and Molecular docking Analysis on Mechanisms and Molecular Targets of LongChaiJiangXue Formula for Treatment of Polycythemia Vera
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Yunyao Jiang, Weiyi Liu, Jing Ming, Yujin Li, Shi-Rong Zhu, and Xiao-Mei Hu
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Polycythemia vera ,Chemistry ,Network pharmacology ,Molecular targets ,Molecular Docking Analysis ,medicine ,Computational biology ,medicine.disease - Abstract
Background: LongChaiJiangXue formula (LCJX) has the effect of not only clearing up excessive erythrocytes but also relieving clinical symptoms of Polycythemia vera (PV). Material/Methods: The chemical constitution of LCJX was identified from Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). Seven hundred and fifty-nine targets were identified and a total of 248 targets were screened out after discarding duplication and genes without any ID in databases. GeneCards database, OMIM database, and GEO database were searched for differential expression genes. The network was built by Cytoscape (3.7.2) software and the protein-protein interaction (PPI) networks of PV and LCJX were merged by https://string-db.org . Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment was processed by the R platform. The molecular docking technology was used to further analyze the intense of the assosiation of the compounds and targets. Results: 73 compounds of LCJX were chosen as the candidate active compounds. The compound-targets network contained 105 nods and 216 edges that presented the interaction of agents and targets. The PPI network of LCJX targets involved 59 nodes and 168 edges. The network showed that the key nodes were concentrated in signal transducer and activator of transcription-3(STAT-3), interleukin-6(IL-6), Janus kinase 2(JAK2), and vascular endothelial growth factor-A(VEGFA). The most enriched terms in the GO analysis in the GO biological processes(BP) were reactive oxygen species metabolic process, response to lipopolysaccharide, and response to oxidative stress. According to GO molecular functions(MF), the central nodes were generally enriched in cytokine receptor binding, cytokine activity, and heme binding. Regarding the GO cell components(CC), the terms included vesicle lumen, cytoplasmic vesicle lumen, and secretory granule lumen. In light of the KEGG enrichment analysis, the Hepatitis B, AGE-RAGE signaling pathway in diabetic complications, Kaposi sarcoma-associated herpesvirus infection, measles, Human cytomegalovirus infection, and JAK-STAT signaling pathway were significantly enriched. The molecular docking technology found that puerarin and saikosaponin A had relative stronger affinity with VEGFA, HIF-1A, JAK2 and STAT3 than other compounds in terms of the binding free energy. Conclusions: The effect of LCJX on PV is achieved through a series of complex mechanisms. Network pharmacology and molecular docking are powerful tools to reveal the effect of compound Chinese medicine on the disease.
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- 2020
17. Oral Arsenic-Containing Qinghuang Powder: A Potential Drug for Myelodysplastic Syndromes
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Jing Ming, Xiao-Mei Hu, Weiyi Liu, Yong-Gang Xu, Haiyan Xiao, and Rou Ma
- Subjects
Oncology ,Drug ,medicine.medical_specialty ,media_common.quotation_subject ,0211 other engineering and technologies ,02 engineering and technology ,Traditional Chinese medicine ,Gene mutation ,030226 pharmacology & pharmacy ,Arsenicals ,Arsenic ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,021105 building & construction ,medicine ,Humans ,Pharmacology (medical) ,Clinical treatment ,media_common ,Aged ,business.industry ,Myelodysplastic syndromes ,General Medicine ,medicine.disease ,Complementary and alternative medicine ,Medicine public health ,Myelodysplastic Syndromes ,Powders ,business ,Drugs, Chinese Herbal - Abstract
Qinghuang Powder (, QHP), an oral arsenic, has become an effective drug in the treatment of myelodysplastic syndromes (MDS) in Xiyuan Hospital, China Academy of Chinese Medical Sciences for many years, and the action mechanism of the compound or active ingredient As2S2 of QHP has been elucidated. Considering the relatively safety, chemotherapy-free and convenient oral profile, QHP is widely used in the clinical treatment for MDS patients, especially for elderly patients. In this review, the authors document the efficacy and safety of oral arsenic-containing compound QHP in the treatment of MDS, with a special focus on the association of efficacy of QHP with the cytogenetics, prognostic risk, DNA methylation, gene mutation, blood arsenic concentration, mechanism of action of As2S2 and the countermeasures against adverse reactions of gastrointestinal tract.
- Published
- 2019
18. Effectiveness evaluation of quota payment for specific diseases under global budget: a typical provider payment system reform in rural China
- Author
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Shihan Lei, Yingchun Chen, Xiao-mei Hu, Di Jiang, Haomiao Li, Yan Zhang, Liangkai Chen, Jingjing Chang, Hongxia Gao, and Dai Su
- Subjects
Budgets ,China ,Quota payment for specific diseases ,Cost Control ,media_common.quotation_subject ,Global budget ,Payment system ,Reimbursement Mechanisms ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Medicine ,030212 general & internal medicine ,health care economics and organizations ,media_common ,Variables ,Actuarial science ,NCRMS ,business.industry ,030503 health policy & services ,Health Policy ,lcsh:Public aspects of medicine ,Reimbursement Mechanism ,lcsh:RA1-1270 ,Payment ,Hospitalization ,Provider payment system reform ,Income ,Linear Models ,Workforce ,Cost control ,Rural Health Services ,Health Expenditures ,0305 other medical science ,business ,Research Article - Abstract
Background Quota payment for specific diseases under global budget is one of the most typical modes of provider payment system reform in rural China. This study aimed to assess this reform mode from aspects of the total fee, structure of the fee and enrollees’ benefits. Methods A total of 127,491 inpatient records from 2014 to 2016 were extracted from the New Rural Cooperative Medical Scheme (NRCMS) database in Weiyuan County, Gansu Province. Total fee, actual compensation ratio, out-of-pocket ratio, constituent ratio of the treatment fee, constituent ratio of the inspection and laboratory fee, and length of stay were selected as dependent variables. Both generalized additive models (GAMs) and multiple linear regression models were used to measure the change in dependent variables along with year. Results Prior to the adjustment of the compensation type, out-of-pocket ratio and length of stay decreased, while total fee, actual compensation ratio, constituent ratio of the treatment fee, and constituent ratio of the inspection and laboratory fee increased. After the compensation type was adjusted, the mean of the total fee increased rapidly in 2015 and remained stable in 2016. The mean length of stay increased in 2015 but decreased in 2016. A comparison of inpatients suffering from diseases covered by quota payments and those suffering from general diseases revealed that total fee, out-of-pocket ratio, and length of stay decreased and actual compensation ratio increased for the former, whereas the opposite was true for the latter. Constituent ratio of the treatment fee and constituent ratio of the inspection and laboratory fee increased for both samples, except for the constituent ratio of the inspection and laboratory fee of quota payment diseases in 2016, which did not change. Conclusions Quota payment for specific diseases under global budget had obviously positive effects on cost control in Weiyuan, Gansu. Considering the limited coverage of quota payment for diseases, the long-term effect of this reform mode and its replicability awaits further evaluation.
- Published
- 2018
19. Design and Evaluation of a Novel Multiplex Real-Time PCR Melting Curve Assay for the Simultaneous Detection of Nine Sexually Transmitted Disease Pathogens in Genitourinary Secretions
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Xiao-Mei Hu, Jiang-Xia Xu, Li-Xia Jiang, Lian-Rui Deng, Zhen-Mei Gu, Xiao-Ying Xie, Hui-Cai Ji, Wei-Hua Wang, Li-Ming Li, Cheng-Nan Tian, Fang-Li Song, Shao Huang, Lei Zheng, and Tian-Yu Zhong
- Subjects
Male ,0301 basic medicine ,Microbiology (medical) ,Sexually transmitted disease ,Bodily Secretions ,sexually transmitted diseases ,polymerase chain reaction ,030106 microbiology ,Immunology ,lcsh:QR1-502 ,Urogenital System ,Mycoplasma hominis ,Real-Time Polymerase Chain Reaction ,urologic and male genital diseases ,medicine.disease_cause ,Sensitivity and Specificity ,Microbiology ,lcsh:Microbiology ,Melting curve analysis ,03 medical and health sciences ,symbols.namesake ,Cellular and Infection Microbiology ,Methods ,medicine ,Humans ,Multiplex ,sanger sequencing ,Sanger sequencing ,biology ,business.industry ,Reproducibility of Results ,biology.organism_classification ,DNA extraction ,Virology ,multiplex ,030104 developmental biology ,Infectious Diseases ,symbols ,Female ,Mycoplasma genitalium ,business ,Multiplex Polymerase Chain Reaction ,pathogen ,Ureaplasma urealyticum - Abstract
Background: Sexually transmitted diseases (STD) are a major cause of infertility, long-term disability, ectopic pregnancy, and premature birth. Therefore, the development of fast and low-cost laboratory STD diagnostic screening methods will contribute to reducing STD-induced reproductive tract damage and improve women's health worldwide. In this study, we evaluated a novel multiplex real-time PCR melting curve assay method for the simultaneous detection of 9 STD pathogens, including Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, Trichomonas vaginalis, Mycoplasma hominis, Ureaplasma urealyticum, Ureaplasma parvum, and herpes simplex virus.Methods: The analytical performance of the method, including its limit of detection (LOD), specificity, repeatability, and effect on different DNA extraction kits were evaluated. Additionally, we obtained 1,328 clinical specimens from 3 hospitals to detect the 9 STD pathogens using multiplex real-time PCR melting curve and Sanger sequencing, to evaluate the sensitivity, specificity, and consistency of the assay method.Results: The results showed that the analytical sensitivity of the novel multiplex real-time PCR melting curve assay is very excellent, with LOD of DNA corresponding to
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- 2019
20. Association of Gene Mutations with Response to Arsenic-Containing Compound Qinghuang Powder (复方青黄散) in Patients with Myelodysplastic Syndromes
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Jun-bin Liang, Mingjing Wang, Jiayue Qin, Xiao-Mei Hu, Pan Zhao, Chong-jian Chen, and Zhongyang Deng
- Subjects
medicine.medical_specialty ,Blood transfusion ,medicine.medical_treatment ,0211 other engineering and technologies ,02 engineering and technology ,Gene mutation ,medicine.disease_cause ,030226 pharmacology & pharmacy ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,021105 building & construction ,medicine ,Effective treatment ,Pharmacology (medical) ,In patient ,Mutation ,business.industry ,Myelodysplastic syndromes ,General Medicine ,medicine.disease ,Complementary and alternative medicine ,Medicine public health ,business ,After treatment - Abstract
To investigate the relationship between gene mutations and response to Compound Qinghuang Powder (复方青黄散, CQHP) in patients with myelodysplastic syndrome (MDS). Forty-three MDS patients were genotyped by ultra-deep targeted sequencing and the clinical data of patients were collected and the relationship between them was analyzed. Up to 41.86% of patients harbored genet mutations, in most cases with more than one mutation. The most common mutations were in SF3B1, U2AF1, ASXL1, and DNMT3A. After treatment with CQHP, about 88.00% of patients no longer required blood transfusion, or needed half of prior transfusions. CQHP is an effective treatment for patients with MDS, especially those with gene mutations in SF3B1, DNMT3A, U2AF1, and/or ASXL1.
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- 2018
21. Effect of Qinghuang Powder (青黄散) Combined with Bupi Yishen Decoction (补脾益肾方) in Treating Patients with Refractory Cytopenia with Multilineage Dysplasia through Regulating DNA Methylation
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De-Xiu Wang, Qing-Bing Zhou, Yong-Gang Xu, Rou Ma, Xiao-Mei Hu, Hongzhi Wang, Xiao-hong Yang, and Fengqin Xu
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medicine.medical_specialty ,Anemia ,0211 other engineering and technologies ,02 engineering and technology ,Neutropenia ,030226 pharmacology & pharmacy ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,hemic and lymphatic diseases ,Internal medicine ,021105 building & construction ,medicine ,Pharmacology (medical) ,business.industry ,Myelodysplastic syndromes ,General Medicine ,Methylation ,medicine.disease ,medicine.anatomical_structure ,Complementary and alternative medicine ,DNA methylation ,Absolute neutrophil count ,Bone marrow ,Refractory cytopenia with multilineage dysplasia ,business - Abstract
To explore the effect of Qinghuang Powder (QHP,(青黄散)combined with Bupi Yishen Decoction (BPYS, 补脾益肾方) on myelodysplastic syndromes (MDS) patients with refractory cytopenia with multilineage dysplasia (RCMD) and determine the change of DNA methylation in MDS-RCMD patients after the treatment of Chinese medicine formula. All 308 MDS-RCMD patients were treated with QHP combined with BPYS for 2 months at least, absolute neutrophil count (ANC), hemoglobin (Hb), platelets (PLT), primitive bone marrow cells and chromosome karyotype were chosen as the main evaluation indexes to analyze the treatment effect according to criteria from the MDS International Working Group. Then 43 bone marrow samples from 15 MDS-RCMD patients and 28 healthy donors were obtained for the examination of DNA methylation. Gene Ontology (GO) and Pathway analysis were applied to analyze the methylation data. The overall MDS response rate to QHP was 61.68% (190/360) including hematologic improvement-neutrophil (HI-N) or hematologic improvement-erythroid (HI-E) or hematologic improvement-platelet (HI-P). Patients with anemia had a better response rate than patients with neutropenia or thrombocypenia (55.88% vs 31.54% or 55.88% vs. 36.9%). The DNA methylation microarray analysis disclosed that 4,257 hypermethylated genes were demethylated upon the treatment with QHP and BPYS. GO analysis and Pathway analysis showed that these demethylated genes were involved in a lot of tumor-related pathways and functions. QHP combined with BPYS could effectively treat MDS-RCMD patients through hematologic improvement (HI-N, HI-P or HI-E) and PLT and RBC transfusion independence due to the demethylation, thereby providing another choice for the treatment of patients with MDS-RCMD.
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- 2018
22. Using circulating tumor DNA to monitor myelodysplastic syndromes status
- Author
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Pan Zhao, Juan Wang, Gang Huang, Xiao-mei Hu, Teng Fan, Jiayue Qin, Haiyan Xiao, Qianze Zhu, and Weiyi Liu
- Subjects
Oncology ,Adult ,Male ,Cancer Research ,medicine.medical_specialty ,Myeloid ,Circulating Tumor DNA ,Internal medicine ,medicine ,Humans ,Letters to the Editor ,Letter to the Editor ,Aged ,business.industry ,Myelodysplastic syndromes ,Hematology ,General Medicine ,Middle Aged ,medicine.disease ,Clinical trial ,Leukemia ,Leukemia, Myeloid, Acute ,medicine.anatomical_structure ,Circulating tumor DNA ,Myelodysplastic Syndromes ,Female ,business - Published
- 2019
23. Prevalence of human papillomavirus infection among 71,435 women in Jiangxi Province, China
- Author
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Yi-Guo Chen, Xiao-Na Fan, Min Lin, Long Li, Rong Hu, Xiao-Ying Xie, Hua-Ping Xiao, Xiao-Mei Hu, Zhao-Xia Liu, Tianyu Zhong, Wei-Hua Wang, and Jichun Zhou
- Subjects
Adult ,China ,medicine.medical_specialty ,Adolescent ,Genotype ,Cervix Uteri ,lcsh:Infectious and parasitic diseases ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Prevalence ,Humans ,Medicine ,lcsh:RC109-216 ,030212 general & internal medicine ,Papillomaviridae ,Cervix ,Aged ,Gynecology ,Cervical cancer ,Human papillomavirus 16 ,Coinfection ,business.industry ,Incidence ,lcsh:Public aspects of medicine ,Incidence (epidemiology) ,Papillomavirus Infections ,Public Health, Environmental and Occupational Health ,HPV infection ,virus diseases ,Cancer ,lcsh:RA1-1270 ,General Medicine ,Middle Aged ,Human papillomavirus 6 ,medicine.disease ,female genital diseases and pregnancy complications ,Vaccination ,Infectious Diseases ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Vagina ,Etiology ,Female ,business - Abstract
Cervical cancer is the third most common cancer in women worldwide. Human papillomavirus (HPV) has been identified as an etiological factor for cervical cancer. Data on the prevalence and subtype distribution of HPV infection in Jiangxi Province are incomplete. In this study, we investigated HPV subtype distribution and prevalence in Jiangxi Province between August 1, 2010, and December 31, 2015. A total of 71,435 individuals ranging in age from 16 to 77 years were recruited. Cervicovaginal swabs were collected from each participant, and HPV screening was performed. Our results showed that the HPV prevalence was 22.49% in Jiangxi Province. Overall, 14.99% of individuals were positive for a single HPV type, and 7.49% were positive for multiple types. The most frequently detected low-risk genotypes were HPV-6, and high-risk genotypes were HPV-16, -18, -33, -52, and -58. The prevalence and type distribution of HPV infection exhibits regional and age differences; Yingtan had the highest incidence for high-risk HPV infection (32.00%), and peaks in the frequencies of HPV infections were seen for patients under 20 and over 60 years of age. In conclusion, we present data showing that the HPV prevalence varies significantly with age and regions in Jiangxi Province. These results can serve as valuable reference to guide Jiangxi cervical cancer screening and HPV vaccination programs. Keywords: Human papillomavirus, Jiangxi Province, Subtypes, Age
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- 2017
24. Predicting bleeding risk in a Chinese immune thrombocytopenia (ITP) population: development and assessment of a new predictive nomogram
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Teng Fan, Yan Sun, Hongzhi Wang, Haiyan Chen, Xiaoqing Guo, Shirong Zhu, Richeng Quan, Xuebin Zhang, Yong-Gang Xu, Xiao-Mei Hu, Mingjing Wang, Weiyi Liu, Xiaoqing Ding, and Yujin Li
- Subjects
0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,Adolescent ,Calibration (statistics) ,lcsh:Medicine ,Feature selection ,Diseases ,Hemorrhage ,Logistic regression ,Article ,03 medical and health sciences ,Predictive nomogram ,Young Adult ,0302 clinical medicine ,Asian People ,Risk Factors ,Internal medicine ,medicine ,Humans ,lcsh:Science ,Bootstrapping (statistics) ,Aged ,Inflammation ,Purpura, Thrombocytopenic, Idiopathic ,Multidisciplinary ,business.industry ,lcsh:R ,Reproducibility of Results ,Regression analysis ,Nomogram ,Middle Aged ,Confidence interval ,Nomograms ,030104 developmental biology ,ROC Curve ,030220 oncology & carcinogenesis ,Cytokines ,lcsh:Q ,Female ,business - Abstract
The aim of this study was to develop a model that could be used to forecast the bleeding risk of ITP based on proinflammatory and anti-inflammatory factors. One hundred ITP patients were recruited to build a new predictive nomogram, another eighty-eight ITP patients were enrolled as validation cohort, and data were collected from January 2016 to January 2019. Four demographic characteristics and fifteen clinical characteristics were taken into account. Eleven cytokines (IFN-γ, IL-1, IL-4, IL-6, IL-8, IL-10, IL-17A, IL-22, IL-23, TNF-α and TGF-β) were used to study and the levels of them were detected by using a cytometric bead array (CBA) human inflammation kit. The least absolute shrinkage and selection operator regression model was used to optimize feature selection. Multivariate logistic regression analysis was applied to build a new predictive nomogram based on the results of the least absolute shrinkage and selection operator regress ion model. The application of C-index, ROC curve, calibration plot, and decision curve analyses were used to assess the discrimination, calibration, and clinical practicability of the predictive model. Bootstrapping validation was used for testing and verifying the predictive model. After feature selection, cytokines IL-1, IL-6, IL-8, IL-23 and TGF-β were excluded, cytokines IFN-γ, IL-4, IL-10, IL-17A, IL-22, TGF-β, the count of PLT and the length of time of ITP were used as predictive factors in the predictive nomogram. The model showed good discrimination with a C-index of 0.82 (95% confidence interval 0.73376–0.90 624) in training cohortn and 0.89 (95% CI 0.868, 0.902) in validation cohort, an AUC of 0.795 in training cohort, 0.94 in validation cohort and good calibration. A high C-index value of 0.66 was reached in the interval validation assessment. Decision curve analysis showed that the bleeding risk nomogram was clinically useful when intervention was decided at the possibility threshold of 16–84%. The bleeding risk model based on IFN-γ, IL-4, IL-10, IL-17A, IL-22, TGF-β, the count of PLT and the length of time of ITP could be conveniently used to predict the bleeding risk of ITP.
- Published
- 2019
25. Genetic alterations in 47 patients with a novel myelodysplastic syndrome diagnosis at a single center
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Pan Zhao, Jiayue Qin, Haiyan Xiao, Yan Lv, Richeng Quan, Xiaoqing Guo, Hongzhi Wang, Juan Wang, Liu Li, Weiyi Liu, Chi Liu, Qianze Zhu, and Xiao-Mei Hu
- Subjects
Cancer Research ,Mutation ,genetic alterations ,Janus kinase 2 ,biology ,Tet methylcytosine dioxygenase 2 ,Cancer ,Articles ,medicine.disease_cause ,medicine.disease ,Molecular biology ,Molecular medicine ,DNA sequencing ,myelodysplastic syndrome ,U2 small nuclear RNA auxiliary factor 1 ,Oncology ,medicine ,biology.protein ,next-generation sequencing ,Gene ,clinicopathological features - Abstract
At least one mutation is present in 70–80% of patients with myelodysplastic syndrome (MDS). Genetic alterations and other molecular biological markers have been included in the diagnostic and treatment guidelines for MDS. The aim of the present study was to analyze the association between genetic alterations and clinicopathological features among 47 Chinese patients with a novel diagnosis of MDS using a next-generation sequencing approach. The results indicated that from the 47 patients, 66.0% had genetic alterations. Furthermore, seven genes, U2 small nuclear RNA auxiliary factor 1 (23.4%), splicing factor 3b subunit (12.8%), ASXL transcriptional regulator 1 (10.6%), tet methylcytosine dioxygenase 2 (8.5%), BCL6 corepressor (8.5%), TP53 (8.5%) and DNA methyltransferase 3α (6.4%), indicated a higher prevalence of alterations in >5% of patients. Among the 16 (51.6%) patients with ≥2 mutations, 12 (75%) had mutations in different genetic functional groups. Variant allele frequencies in signaling pathways were generally low, suggesting that mutations in the corresponding genes were acquired relatively late during the evolution of the leukemic clones. The mutation prevalence rates of Janus kinase 2 and SH2B adaptor protein 3 were significantly higher in the MDS unclassified group and in the very high-risk groups with a karyotype as a prognostic indicator, respectively (both P
- Published
- 2019
26. Effect of inappropriate admissions on hospitalization performance in county hospitals: a cross-sectional study in rural China
- Author
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Zhifang Chen, Yingchun Chen, Min Tan, Dai Su, Di Jiang, Shihan Lei, Yan Zhang, Jingjing Chang, Haomiao Li, Hongxia Gao, and Xiao-mei Hu
- Subjects
medicine.medical_specialty ,Cross-sectional study ,Excessive utilization of health services ,Health administration ,03 medical and health sciences ,0302 clinical medicine ,Clinical pathway ,Propensity score matching ,medicine ,Appropriateness evaluation protocol ,030212 general & internal medicine ,lcsh:R5-920 ,Health economics ,business.industry ,Research ,030503 health policy & services ,Health Policy ,Public health ,Medical record ,Health services research ,Inappropriate admissions ,Emergency medicine ,lcsh:Medicine (General) ,0305 other medical science ,business - Abstract
Background Inappropriate admissions cause excessive utilization of health services compared with outpatient services. However, it is still unclear whether inappropriate admissions cause excessive use of health services compared with appropriate admissions. This study aims to clarify the differences in the hospitalization performances between appropriately admitted inpatients and inappropriately admitted inpatients. Methods A total of 2575 medical records were obtained after cluster sampling in three counties. Admission appropriateness was assessed by appropriateness evaluation protocol (AEP). The propensity score matching (PSM) was computed to match patients in treatment and control group with similar characteristics, and to examine the differences in the utilization of hospitalization services between the two groups. The samples were matched in two major steps in this study. In the first step, total samples were matched to examine the differences in the utilization of hospital services between the two groups using 15 individual covariates. In the second step, PSM was computed to analyze the differences between the two groups in different disease systems using 14 individual covariates. Results For the whole sample, the inappropriate group has lower expenditure of hospitalization (EOH) (difference = − 0.12, p = 0.003) and shorter length of stay (LOS) (difference = − 0.73, p = 0.016) than the appropriate group. For number of clinical inspection (NCI), it has no statistically significant difference (difference = − 0.39, p = 0.082) between the two groups. Among different disease systems, no significant differences were observed between the two groups among EOH, LOS and NCI, except that the EOH was lower in the inappropriate group than that in the appropriate group for surgical disease (difference = − 0.169, p = 0.043). Conclusion Inappropriate admissions have generated excessive health service utilization compared with appropriate admissions, especially for internal diseases. The departments in charge of medical services and hospital managers should pay high attention to the health service utilization of the inappropriately admitted inpatients. Relevant medical policies should be designed or optimized to increase the appropriateness in health care service delivery and precision in clinical pathway management.
- Published
- 2019
27. Is There a Difference in the Utilisation of Inpatient Services Between Two Typical Payment Methods of Health Insurance? Evidence from the New Rural Cooperative Medical Scheme in China
- Author
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Di Jiang, Shihan Lei, Yingchun Chen, Hongxia Gao, Zhifang Chen, Haomiao Li, Xiao-mei Hu, Dai Su, Min Tan, and Jingjing Chang
- Subjects
Hospitals, County ,Rural Population ,Matching (statistics) ,China ,interrupted time-series analysis ,new rural cooperative medical scheme ,Health, Toxicology and Mutagenesis ,media_common.quotation_subject ,difference ,lcsh:Medicine ,payment method ,Article ,03 medical and health sciences ,0302 clinical medicine ,Health insurance ,Medicine ,Humans ,030212 general & internal medicine ,media_common ,Inpatients ,Insurance, Health ,propensity score matching ,business.industry ,030503 health policy & services ,lcsh:R ,Public Health, Environmental and Occupational Health ,Payment ,Hospitals ,Medical services ,utilisation of inpatient services ,Hospitalization ,Propensity score matching ,Rural Health Services ,Health Expenditures ,0305 other medical science ,business ,Delivery of Health Care ,Demography - Abstract
This study aimed to evaluate the effects of the differences between two typical payment methods for the new rural cooperative medical scheme (NRCMS) in China on the utilisation of inpatient services. Interrupted time-series analysis (ITSA) and propensity score matching (PSM) were used to measure the difference between two typical payment methods for the NRCMS with regard to the utilisation of inpatient services. After the reform was formally implemented, the level and slope difference after reform compared with pre-intervention (distribution of inpatients in county hospitals (DIC), distribution of inpatients in township hospitals (DIT) and the actual compensation ratio of inpatients (ARCI)) were not statistically significant. Kernel matching obtained better results in reducing the mean and median of the absolute standardised bias of covariates of appropriateness of admission (AA), appropriateness of disease (AD). The difference in AA and AD of the matched inpatients between two groups was &minus, 0.03 (p-value = 0.042, 95% CI: &minus, 0.08 to 0.02) and 0.21 (p-value <, 0.001, 95% CI: &minus, 0.17 to 0.25), respectively. The differences in the utilisation of inpatient services may arise owing to the system designs of different payment methods for NRCMS in China. The causes of these differences can be used to guide inpatients to better use medical services, through the transformation and integration of payment systems.
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- 2019
28. Effect of integrated urban and rural residents medical insurance on the utilisation of medical services by residents in China: a propensity score matching with difference-in-differences regression approach
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Hongxia Gao, Shihan Lei, Di Jiang, Xiao-mei Hu, Haomiao Li, Yingchun Chen, Min Tan, Jingjing Chang, Zhifang Chen, and Dai Su
- Subjects
Male ,Rural Population ,Matching (statistics) ,Longitudinal study ,China ,Urban Population ,Average treatment effect ,urban and rural residents medical insurance ,Treatment and control groups ,03 medical and health sciences ,0302 clinical medicine ,Ambulatory care ,Environmental health ,Medicine ,Humans ,030212 general & internal medicine ,Longitudinal Studies ,Healthcare Disparities ,Propensity Score ,Aged ,Inpatients ,Insurance, Health ,difference-in-differences regression ,Inpatient care ,propensity score matching ,business.industry ,030503 health policy & services ,Research ,utilisation of medical services ,General Medicine ,Health Services ,Middle Aged ,Difference in differences ,Hospitalization ,Logistic Models ,Propensity score matching ,Female ,Health Services Research ,0305 other medical science ,business - Abstract
ObjectivesIn this study, we aim to evaluate the effect of urban and rural resident medical insurance scheme (URRMI) on the utilisation of medical services by urban and rural residents in the four pilot provinces.Setting and participantsThe sample used in this study is 13 305 individuals, including 2620 in the treatment group and 10 685 in the control group, from the 2011 and 2015 surveys of China Health and Retirement Longitudinal Study.Outcome measuresPropensity score matching and difference-in-differences regression approach (PSM-DID) is used in the study. First, we match the baseline data by using kernel matching. Then, the average treatment effect of the four outcome variables are analysed by using the DID model. Finally, the robustness of the PSM-DID estimation is tested by simple model and radius matching.ResultsKernel matching have improved the overall balance after matching. The URRMI policy has significantly reduced the need-but-not outpatient care and significantly increased outpatient care cost and inpatient care cost for rural residents, with DID value of −0.271, 0.090 and 0.256, respectively. After robustness test, the DID competing results of four outcome variables are consistent.ConclusionsURRMI has a limited effect on the utilisation of medical and health services by all residents, but the effect on rural residents is obvious. The government should establish a unified or income-matching payment standard to prevent, control the use of medical insurance funds and increase its efforts to implement URRMI integration in more regions to improve overall fundraising levels.
- Published
- 2019
29. Determinants of inappropriate admissions of children to county hospitals: a cross-sectional study from rural China
- Author
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Shihan Lei, Yingchun Chen, Haomiao Li, Qing Ye, Yan Zhang, Di Jiang, Jingjing Chang, Xiao-mei Hu, and Dai Su
- Subjects
Hospitals, County ,Male ,Cross-sectional study ,Respiratory Tract Diseases ,Rural Health ,Logistic regression ,Health administration ,0302 clinical medicine ,Surveys and Questionnaires ,Outpatients ,Ambulatory Care ,030212 general & internal medicine ,Inappropriate admission ,Child ,Children ,lcsh:Public aspects of medicine ,030503 health policy & services ,Health Policy ,Medical record ,Incidence (epidemiology) ,Questionnaire ,Hospitals, Pediatric ,Stratified sampling ,Hospitalization ,Child, Preschool ,Female ,0305 other medical science ,Research Article ,medicine.medical_specialty ,China ,Adolescent ,Health Services Misuse ,Infections ,County hospital ,03 medical and health sciences ,medicine ,Humans ,Retrospective Studies ,Primary Health Care ,business.industry ,Public health ,Infant, Newborn ,Infant ,lcsh:RA1-1270 ,Length of Stay ,Cross-Sectional Studies ,Logistic Models ,Family medicine ,Rural China ,business ,Facilities and Services Utilization - Abstract
Background The incidence of inappropriate admissions in China has become the shackle of its’ service supply system. This research aims to assess the level of children’s inappropriate admissions to county hospitals in rural China and identify the characteristics and determinants of children’s inappropriate admissions. Methods A retrospective review was conducted on data of children aged 0–14 years. A total of 771 children medical records in four county hospitals was collected by stratified random sampling in Midwestern China and was evaluated through the Rural Appropriateness Evaluation Protocol. A questionnaire survey was conducted among doctors whose names were shown in medical records. Chi-square test was used to analyse the characteristics of inappropriate admissions, and a binary logistic regression model was used to examine the determinants of inappropriate admissions. Results Inappropriate admissions indicate that patients who could have been treated as outpatients received services as inpatients. The average rate for inappropriate admissions of children in county hospitals was 61.35%. The highest rate of inappropriate admissions was found among children aged 1–5 years (68.42%). Inappropriate admissions mostly occurred in children with respiratory diseases (72.45%), circulatory diseases (72.22%) and certain infectious diseases and parasitic diseases (70.37%). Binary logistic regression analysis showed that county, normal health status, treating department, disease, the length of hospital stay and the doctor’s self-evaluation on the understanding about the degree of the patient’s feelings were determinants for children’s inappropriate admissions. Conclusions County hospitals have a high rate of inappropriate admissions of children. The relationship of children’s inappropriate admissions to age distribution and the insurance compensation is affected by disease and hospitalisation expenses, respectively. The determinants of children’s inappropriate admissions are directly related to the weak level of primary care services in the health service system, the initial requirements requested by children’s admission decision makers and the interests among medical institutions and doctors. Electronic supplementary material The online version of this article (10.1186/s12913-019-3944-1) contains supplementary material, which is available to authorized users.
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- 2019
30. Does County-Level Medical Centre Policy Influence the Health Outcomes of Patients with Trauma Transported by the Emergency Medical Service System? An Integrated Emergency Model in Rural China
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Min Tan, Shihan Lei, Hongxia Gao, Xiao-mei Hu, Haomiao Li, Jingjing Chang, Di Jiang, Yingchun Chen, Dai Su, and Zhifang Chen
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Male ,Rural Population ,medicine.medical_specialty ,China ,Emergency Medical Services ,Waist ,Health, Toxicology and Mutagenesis ,patients with trauma transported by EMS ,lcsh:Medicine ,Transportation ,Health outcomes ,Article ,03 medical and health sciences ,0302 clinical medicine ,Injury Severity Score ,Trauma Centers ,medicine ,Humans ,health outcomes ,030212 general & internal medicine ,County level ,Limb injury ,Propensity Score ,Proportional Hazards Models ,Retrospective Studies ,propensity score matching ,Proportional hazards model ,business.industry ,Health Policy ,Hazard ratio ,Head injury ,lcsh:R ,Public Health, Environmental and Occupational Health ,030208 emergency & critical care medicine ,medicine.disease ,Hospitals ,Treatment Outcome ,Cox proportional hazard model ,Emergency medicine ,Propensity score matching ,Female ,business ,county-level medical centre - Abstract
This study aimed to assess the effect of the county-level medical centre policy on the health outcomes of trauma patients transported by emergency medical service (EMS) system in rural China. The methodology involved the use of electronic health records (EHRs, after 2016) of patients with trauma conditions such as head injury (n = 1931), chest (back) injury (n = 466), abdominal (waist) injury (n = 536), and limb injury (n = 857) who were transported by EMS to the county-level trauma centres of Huining County and Huan County in Gansu, China. Each patient was matched with a counterpart to a county-level trauma centre hospital by propensity score matching. Cox proportional hazard models were used to estimate the hazard ratios (HRs) of such patients in different hospitals. The HRs of all patients with the abovementioned traumatic conditions transported by EMS to county-level trauma centre hospitals were consistently higher than those transported by EMS to traditional hospitals after adjusting for numerous potential confounders. Higher HRs were associated with all patients with trauma (HR = 1.249, p <, 0.001), head injury (HR = 1.416, p <, 0.001), chest (back) injury (HR = 1.112, p = 0.560), abdominal (waist) injury (HR = 1.273, p = 0.016), and limb injury (HR = 1.078, p = 0.561) transported by EMS to the county-level trauma centre hospitals. Our study suggests that the construction of county-level medical centre provides an effective strategy to improve the health outcomes of EMS-transported trauma patients in Gansu, China. Policy makers can learn from the experience and improve the health outcomes of such patients through a personalised trauma treatment system and by categorizing the regional trauma centre.
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- 2019
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31. Clinical characteristics and treatment outcome of COVID-19 patients with stroke in China: A multicenter retrospective study
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Yanping Wang, Ning Liang, Hai jun Xie, Yun hong Hu, Yingchun Zhou, Lin song Zhang, H. S. Chen, Yan Ma, Jing wei Wang, Ren bo Chen, Shuai Shi, Xin yu Ji, Hui Na, Xiao mei Hu, Yu dong Wang, Si hong Liu, Huamin Zhang, Min qing Li, Gui hui Wu, Yong yan Wang, Nan Nan Shi, Zhang Liu, and Ji ke Li
- Subjects
Male ,Treatment outcome ,Pharmaceutical Science ,Fibrinogen ,0302 clinical medicine ,Epidemiology ,Drug Discovery ,COVID-19, novel coronavirus disease 2019 ,Stroke ,Aged, 80 and over ,0303 health sciences ,LDH, lactate dehydrogenase ,Age Factors ,Blood Coagulation Disorders ,Middle Aged ,ICU, intensive care unit ,CT, computed tomography ,030220 oncology & carcinogenesis ,Molecular Medicine ,Original Article ,Female ,Abnormal laboratory findings ,medicine.drug ,Adult ,medicine.medical_specialty ,TIA, transient ischemic attack ,China ,Coronavirus disease 2019 (COVID-19) ,SARS-CoV, severe acute respiratory syndrome coronavirus ,Antiviral Agents ,PaO2, arterial partial pressure of oxygen ,03 medical and health sciences ,Internal medicine ,medicine ,Humans ,030304 developmental biology ,Aged ,Retrospective Studies ,Pharmacology ,Clinical characteristics ,business.industry ,Oxygen Inhalation Therapy ,COVID-19 ,Retrospective cohort study ,medicine.disease ,Stroke in China ,Complementary and alternative medicine ,business - Abstract
Objective Previous studies mainly reported the clinical characteristics of novel coronavirus 2019 (COVID-19) infections, but the research on clinical characteristics and treatment outcomes of COVID-19 patients with stroke is still rare. Methods A multi-center retrospective study was conducted at 11 hospitals in 4 provinces of China, and COVID-19 patients with stroke were enrolled from February 24 to May 4, 2020. We analyzed epidemiological, demographic, and clinical characteristics of cases as well as the laboratory test results, treatment regimens and outcomes, and the clinical characteristics and therapeutic outcomes were compared between severe and nonsevere patients, and by age group, respectively. Results A total of 27 patients [mean age: 66.41 (SD 12.1) years] were enrolled. Among them, 9 (33.3%) were severe patients and 18 (66.7%) were nonsevere patients; 17 (63.0%) were female; 19 (70.4%) were aged 60 years and above. The most common symptoms were fever [19 (70.4%)], fatigue [12 (44.4%)] and cough [11 (40.7%)], respectively. Abnormal laboratory findings of COVID-19 patients with stroke included high levels of C-reactive protein [19 (73.1%)], D-dimer [14 (58.3%)], blood glucose [14 (53.8%)], fibrinogen [13 (50.0%)], and decreased lymphocytes [12 (44.4%)]. Comparing to nonsevere cases with stroke, severe patients with stroke were likely to be older, susceptible to receiving oxygen inhalation, and had more complications (p < 0.05). In addition, there were significant differences in lymphocytes, neutrophils, lactate dehydrogenase, C-reactive protein, creatine kinase between the severe cases and nonsevere cases (p < 0.05). The older patients had a decreased platelet count and elevated fibrinogen, compared with the younger (p < 0.05). All patients (100%) received antiviral treatment, 12 (44.4%) received antibiotics treatment, 26 (96.3%) received Traditional Chinese Medicine (Lung cleansing & detoxifying decoction), and oxygen inhalation was in 18 (66.7%). The median duration of hospitalization was 16 days. By May 4, 2020, a total of 26 (96.3%) patients were cured and discharged, and 1 (3.7%) patients died. Conclusion COVID-19 patients with stroke had poor indicators of coagulation system, and severe and older patients might have a higher risk of complications and unfavorable coagulation system. However, the overall treatment outcome is favorable., Graphical abstract Image, graphical abstract
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- 2020
32. Application of the combination of 1,3-dichloropropene and dimethyl disulfide by soil injection or chemigation: effects against soilborne pests in cucumber in China
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Qiuxia Wang, Yuan Li, Shen Jin, Liangang Mao, Wensheng Fang, Xiao-mei Hu, Ouyang Canbin, Guo Meixia, Liu Pengfei, Dongdong Yan, and Aocheng Cao
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0106 biological sciences ,dimethyl disulfide ,Fertigation ,Agriculture (General) ,Greenhouse ,Plant Science ,medicine.disease_cause ,01 natural sciences ,Biochemistry ,S1-972 ,chemistry.chemical_compound ,1,3-Dichloropropene ,Food Animals ,Infestation ,Botany ,Fusarium oxysporum ,Meloidogyne incognita ,medicine ,Dimethyl disulfide ,1,3-dichloropropene ,Ecology ,biology ,fungi ,food and beverages ,soil fumigation ,soilborne pest ,biology.organism_classification ,010602 entomology ,Horticulture ,chemistry ,Animal Science and Zoology ,Phytophthora ,Agronomy and Crop Science ,cucumber ,010606 plant biology & botany ,Food Science - Abstract
The combination of 1,3-dichloropropene+dimethyl disulfide (1,3-D+DMDS), which forms a pre-plant soil fumigant, can provide a substitute for the environmentally unfriendly methyl bromide (MB). Three greenhouse trials were performed to evaluate the root-knot nematode and soilborne fungi control efficacy in the suburbs of Beijing in China in 2010–2014. Randomized blocks with three replicates were designed in each trial. The combination of 1,3-D+DMDS (10+30 g m−2) significantly controlled Meloidogyne incognita, effectively suppressed the infestation of Fusarium oxysporum and Phytophthora spp., and successfully provided high commercial fruit yields (equal to MB but higher than 1,3-D or DMDS). The fumigant soil treatments were significantly better than the untreated controls. These results indicate that 1,3-D+DMDS soil treatments can be applied by soil injection or chemigation as a promising MB alternative against soilborne pests in cucumber in China.
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- 2016
33. Therapeutic Targeting of the Staphylococcus aureus Accessory Gene Regulator (agr) System
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Shu Li, Si Rui Li, Xiao Mei Hu, Bei Jiang, and Li Tan
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0301 basic medicine ,Microbiology (medical) ,Accessory gene ,Staphylococcus aureus ,Mini Review ,agr ,030106 microbiology ,lcsh:QR1-502 ,virulence factors ,Regulator ,Virulence ,Biology ,Therapeutic targeting ,medicine.disease_cause ,Microbiology ,lcsh:Microbiology ,Pathogenesis ,03 medical and health sciences ,autoinduction ,medicine ,Biofilm ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,Quorum sensing ,bacteria ,biofilms - Abstract
Staphylococcus aureus can cause numerous different diseases, which has been attributed to its large repertoire of virulence factors, many of which are under the control of the accessory gene regulator (agr) quorum sensing system. Under conditions of high cell density, agr increases the production of many virulence factors, decreases expression of several colonization factors, and is intimately associated with the pathogenesis and biofilm formation of S. aureus. This review summarizes our current understanding of the molecular mechanisms underlying agr quorum sensing and the regulation of agr expression. The discussion also examines subgroups of agr and their association with different diseases, and concludes with an analysis of strategies for designing drugs and vaccines that target agr to combat S. aureus infections.
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- 2018
34. An Efficient Process for Pretreatment of Lignocelluloses in Functional Ionic Liquids
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Shi-Jia Dong, Yun-Fei Gao, Bixian Zhang, and Xiao-Mei Hu
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Article Subject ,Polymers and Plastics ,Extraction (chemistry) ,Inorganic chemistry ,Lignocellulosic biomass ,lcsh:Chemical technology ,Chloride ,chemistry.chemical_compound ,Crystallinity ,chemistry ,Enzymatic hydrolysis ,Ionic liquid ,medicine ,Lignin ,lcsh:TP1-1185 ,Fourier transform infrared spectroscopy ,medicine.drug - Abstract
Background and Aims. The complex structure of the lignocelluloses is the main obstacle in the conversion of lignocellulosic biomass into valuable products. Ionic liquids provide the opportunities for their efficient pretreatment for biomass. Therefore, in this work, pretreatment of corn stalk was carried out in ultrasonic-assisted ionic liquid including 1-butyl-3-methylimidazolium chloride [BMIM]Cl, 1-H-3-methylimidazolium chloride [HMIM]Cl, and 1-(1-propylsulfonic)-3-imidazolium chloride [HSO3-pMIM]Cl at 70°C for 2 h. We compared the pretreatments by ionic liquid with and without the addition of deionized water. Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM) were employed to analyze the chemical characteristics of regenerated cellulose-rich materials.Results. [HMIM]Cl and [HSO3-pMIM]Cl were effective in lignin extraction to obtain cellulose-rich materials. FTIR analysis and SEM analysis indicated the effective lignin removal and the reduced crystallinity of cellulose-rich materials. Enzymatic hydrolysis of cellulose-rich materials was performed efficiently. High yields of reducing sugar and glucose were obtained when the corn stalk was pretreated by [HMIM]Cl and [HSO3-pMIM]Cl.Conclusions. Ionic liquids provided the ideal environment for lignin extraction and enzymatic hydrolysis of corn stalk and [HMIM]Cl and [HSO3-pMIM]Cl proved the most efficient ionic liquids. This simple and environmentally acceptable method has a great potential for the preparation of bioethanol for industrial production.
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- 2015
35. Effect of an Integrated Payment System on the Direct Economic Burden and Readmission of Rural Cerebral Infarction Inpatients: Evidence from Anhui, China
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Haomiao Li, Shihan Lei, Xiao-mei Hu, Min Tan, Yingchun Chen, Zhifang Chen, Jingjing Chang, Dai Su, Hongxia Gao, and Di Jiang
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Budgets ,Rural Population ,China ,Total cost ,Health, Toxicology and Mutagenesis ,lcsh:Medicine ,Payment system ,Logistic regression ,Health outcomes ,Patient Readmission ,Article ,03 medical and health sciences ,0302 clinical medicine ,Cost of Illness ,medicine ,Humans ,030212 general & internal medicine ,health care economics and organizations ,Inpatients ,Delivery of Health Care, Integrated ,direct economic burden ,readmission ,business.industry ,Cerebral infarction ,030503 health policy & services ,lcsh:R ,Public Health, Environmental and Occupational Health ,Odds ratio ,cerebral infarction ,Readmission rate ,medicine.disease ,rural China ,Hospitalization ,integrated payment system ,Compensation and Redress ,Female ,Health Expenditures ,0305 other medical science ,business ,Demography - Abstract
Rural China is piloting an integrated payment system, which prepays a budget to a medical alliance rather than a single hospital. This study aims to evaluate the effect of this reform on the direct economic burden and readmission rates of cerebral infarction inpatients. The settlement records of 78,494 cerebral infarction inpatients were obtained from the New Rural Cooperative Medical Scheme (NRCMS) database in Dingyuan and Funan Counties in the Anhui Province. The direct economic burden was estimated by total costs, out-of-pocket expenditures, the out-of-pocket ratio, and the compensation ratio of the NRCMS. Generalized additive models and multivariable linear/logistic regression were applied to measure the changes of the dependent variables along with the year. Within the county, the total costs positively correlated to the year (&beta, = 313.10 in 2015, 163.06 in 2016). The out-of-pocket expenditures, out-of-pocket ratios, and the length-of-stay positively correlated to the year in 2015 (&beta, = 105.10, 0.01, and 0.18 respectively), and negatively correlated to the year in 2016 (&beta, = &minus, 58.40, &minus, 0.03, and &minus, 0.30, respectively). The odds ratios of the readmission rates were less than one within the county (0.70 in 2015, 0.53 in 2016). The integrated payment system in the Anhui Province has considerably reduced the direct economic burden for the rural cerebral infarction inpatients, and the readmission rate has decreased within the county. Inpatients&rsquo, health outcomes should be given further attention, and the long-term effect of this reform model awaits further evaluation.
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- 2019
36. Determinants of inappropriate admission of elderly people in county-level hospitals: a cross-sectional study in rural China
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Dai Su, Hongxia Gao, Xiao-mei Hu, Haomiao Li, Di Jiang, Shihan Lei, Yingchun Chen, Yan Zhang, and Jingjing Chang
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Adult ,Hospitals, County ,Male ,China ,medicine.medical_specialty ,rural china ,Cross-sectional study ,health care facilities, manpower, and services ,Logistic regression ,elderly ,quality in health care ,Social group ,03 medical and health sciences ,Patient Admission ,0302 clinical medicine ,Humans ,Medicine ,Elderly people ,030212 general & internal medicine ,County level ,Health policy ,Aged ,business.industry ,Health Policy ,Research ,030503 health policy & services ,Age Factors ,inappropriate admission ,Regression analysis ,social sciences ,General Medicine ,Middle Aged ,humanities ,Cross-Sectional Studies ,Health Care Surveys ,Family medicine ,Female ,Rural Health Services ,0305 other medical science ,business - Abstract
ObjectiveThe purpose of this paper is to investigate the characteristics and determinants of inappropriate admission to hospital of elderly people in rural China.DesignA cross-sectional study of a comparison between the elderly and non-elderly groups of people.SettingThe survey was conducted on the largest county-level general hospitals in four counties in central and western China.ParticipantsA total of 652 rural patients admitted in hospitals were surveyed, who were divided into two groups according to age: elderly group (n=230, age ≥60 years) and non-elderly group (n=422, age Primary measuresThe Chinese version of the appropriateness evaluation protocol was used to evaluate the inappropriate admission rates. The interactive regression models based on the relationship of age (elderly and non-elderly) with other factors and binary logistic regression models were used in the analysis of the specific factors and determinants of the inappropriate admission of elderly people.ResultThe inappropriate admission rate for the rural elderly was 30%, which was lower than that of the non-elderly people (40.8%). Compared with the non-elderly group, women in the elderly group (OR=0.33, 95% CI 0.15 to 0.73) had a lower incidence of inappropriate admission, and elderly people with chronic diseases (OR=3.33, 95% CI 1.23 to 9.04) were more prone to being inappropriately admitted than non-elderly people with chronic diseases. The binary logistic regression analysis shows that county, age (OR=0.94. 95% CI 0.90 to 0.99), gender (OR=0.49, 95% CI 0.25 to 2.98), department and response to doctor’s admission request were the determinants of the inappropriate admission of elderly patients.ConclusionThe inappropriate admission rate of elderly people in rural China was high. We found that gender and chronic disease are the specific factors that were specific to non-elderly people. County, age, gender, department and response to a doctor’s admission request had substantial influence on the inappropriate admission of the elderly in rural China.
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- 2019
37. Simulation of Wind Speed in Campus Based on FLUENT
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Tao Yu, Yu Jin Chao, and Xiao Mei Hu
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Urban surface ,Engineering ,Meteorology ,business.industry ,Fluent software ,General Engineering ,Air pollution ,Distribution law ,Particulates ,medicine.disease_cause ,Wind speed ,Air pollutants ,medicine ,Fluent ,business - Abstract
In order to study urban surface dust distribution, a three-dimensional model is proposed and the distribution of wind and the distribution law of air pollutants are researched. 3D campus of Shanghai University as a typical area is selected. The standard height (Twenty two meters) of teaching building for reference has been chosen. In different distances such as Eight、Twelve、Fifteen、Twenty meters, the amount and distribution of wind speed were obtained from simulation using FLUENT software. Combined with the particulate of air pollution’s distribution in urban surface, the optimized design of the main campus’s building spacing is analyzed. The results show that when the building space is about Fifteen meters, the concentration of pollutants and the distribution of wind speed is optimized.
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- 2013
38. Rural-Urban Differences of Dietary Patterns, Overweight, and Bone Mineral Status in Chinese Students
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Xiao-Mei Hu, Tian-Jiao Chen, Yang Yang, and Ming-Jie Bai
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Gerontology ,Male ,Rural Population ,Urban Population ,Blood Pressure ,Overweight ,risk of bone fracture ,dietary pattern ,overweight/obesity ,central adiposity ,adolescent students ,rural-urban disparity ,Body Mass Index ,Fractures, Bone ,0302 clinical medicine ,Bone Density ,Risk Factors ,Prevalence ,030212 general & internal medicine ,Child ,Bone mineral ,Nutrition and Dietetics ,Schools ,Confounding ,Hypertension ,Central Adiposity ,Female ,medicine.symptom ,lcsh:Nutrition. Foods and food supply ,Westernization ,China ,Adolescent ,education ,030209 endocrinology & metabolism ,lcsh:TX341-641 ,Article ,03 medical and health sciences ,Asian People ,Environmental health ,medicine ,Humans ,Obesity ,Students ,business.industry ,medicine.disease ,Cross-Sectional Studies ,Socioeconomic Factors ,Diet, Western ,Rural area ,business ,Food Science - Abstract
China is an urban and rural social model country. In the past three decades, the developing speed of rural areas has been much slower than urban areas, which may lead to the differences in dietary patterns. This study aimed to investigate the disparities of dietary structures from urban and rural children, and to analyze the effects of different dietary patterns on their adverse outcome. Among 1590 students, aged 11 years to 17 years, from primary and middle schools, a cross-sectional study was conducted. There were three dietary patterns recognized: Westernization structure, meat diet structure, and Western and Chinese structure. Compared with rural students, more urban students were in the highest categories of the whole dietary patterns (p < 0.001). Overweight/obesity and central adiposity were more prevailing among urban students, while rural students had a more prevailing risk of bone fracture (p < 0.05). Through the adjustment for all confounding factors, the Westernization structure could increase the risk of overweight/obesity and central adiposity, the meat structure could increase the risk of elevated blood pressure/hypertension, while the risk of low bone mineral quality could be reduced by the Chinese and Western structure. In conclusion, a rural-urban disparity in dietary patterns was found in our study, and different dietary patterns were associated with the risk of some adverse outcomes. Therefore, there were different prevalences of the adverse outcomes between rural and urban students.
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- 2016
39. Twins methods quantitatively explore the genetic impact on children and adolescents brain gray matter volume
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Liansheng Zhao, Xiaolu Chen, Qian He, Xiao-Mei Hu, Xiao-Wei Zhang, Yixiao Fu, Huaqing Meng, Xiao Li, Tao Li, Yingcheng Wang, Xiao Hou, and Xingshun Ma
- Subjects
Cingulate cortex ,Male ,Adolescent ,Science ,Twins ,Biology ,Gray (unit) ,Article ,Temporal lobe ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Gray Matter ,Child ,Multidisciplinary ,Parietal lobe ,Anatomy ,Environmental exposure ,Environmental Exposure ,Magnetic Resonance Imaging ,030227 psychiatry ,Frontal lobe ,Medicine ,Female ,Brain Gray Matter ,Occipital lobe ,Genetic Background ,030217 neurology & neurosurgery - Abstract
The gray matter volumes of 58 pairs of twins ranging in age from 12 to 18 were measured by MRI to explore the genetic and environmental impacts on gray matter volume in twin children and adolescents. By means of A/C/E structural equation modeling, it was found that the gray matter volume in children and adolescents was jointly affected by genetic (A: 0.89) and environmental factors while genetic factors play a greater role. The gray matter volume in frontal lobe, parietal lobe, occipital lobe and lateral temporal lobe was mainly affected by genetics (A: 0.7–0.89), where as the gray matter volume in medial temporal lobe and cingulate cortex was affected by both genetics and environment.
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- 2016
40. Clinical observation of the treatment of myelodysplastic syndrome mainly with Qinghuang Powder (青黄散)
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Yong-Gang Xu, Rou Ma, Shu Xu, Xiao-Mei Hu, Shu-zhen Sun, Hongzhi Wang, Feng Liu, and Xiao-hong Yang
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medicine.medical_specialty ,Pathology ,Clinical effectiveness ,business.industry ,Myelodysplastic syndromes ,Treatment outcome ,General Medicine ,medicine.disease ,Gastroenterology ,Clinical trial ,Complementary and alternative medicine ,Internal medicine ,Medicine public health ,medicine ,Pharmacology (medical) ,business - Abstract
Objective To observe the clinical effectiveness of Qinghuang Powder (青黄散, QHP) combined with Bupi Yishen Decoction (补脾益肾汤, BPYS) in treating myelodysplastic syndrome (MDS), and its relationship with France, America, and Britain (FAB) type, international prognosis scaling system (IPSS) risk, and chromosome karyotype.
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- 2011
41. Application and assessment of Chinese arsenic drugs in treating malignant hematopathy in China
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Feng Liu, Rou Ma, and Xiao-Mei Hu
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inorganic chemicals ,Acute promyelocytic leukemia ,China ,chemistry.chemical_element ,Traditional Chinese medicine ,Pharmacology ,Arsenicals ,chemistry.chemical_compound ,Arsenic Trioxide ,medicine ,Humans ,Pharmacology (medical) ,Arsenic disulfide ,Clinical efficacy ,Arsenic trioxide ,Adverse effect ,Multiple myeloma ,Arsenic ,integumentary system ,business.industry ,Oxides ,General Medicine ,medicine.disease ,Complementary and alternative medicine ,chemistry ,Hematologic Neoplasms ,business ,Drugs, Chinese Herbal - Abstract
Chinese arsenic drugs have been applied in Chinese medicine for several centuries. Beginning from 1970s, arsenic containing drugs have been generally used for the treatment of malignant hematologic diseases including acute promyelocytic leukemia, myelodysplastic syndrome, and multiple myeloma. No matter what ingredients of arsenic drugs were applied, either arsenic trioxide, arsenic disulfide, or arsenic containing Chinese herbal compositions including Qinghuang Powder and Realgar-Indigo naturalis formula, they all provided the distinct approaches for the management of malignant hematologic diseases, and good clinical efficacy was obtained with mild adverse reactions. Moreover, the mechanisms of action have been continually elucidated.
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- 2010
42. Effect and prognostic analysis of treatment for acute myeloid leukemia using Chinese drugs combined with chemotherapy
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Feng Liu, Xiao-hong Yang, Chi Liu, Nai-ping Hu, Liu Li, Hongzhi Wang, Haiyan Xiao, Chun-mei Zheng, Rou Ma, Xiao-Mei Hu, Shan-shan Zhang, and Yong-Gang Xu
- Subjects
Adult ,Male ,Oncology ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,Immunophenotyping ,Young Adult ,Pharmacotherapy ,hemic and lymphatic diseases ,Internal medicine ,Antineoplastic Combined Chemotherapy Protocols ,medicine ,Humans ,Pharmacology (medical) ,Clinical efficacy ,Child ,neoplasms ,Aged ,Etoposide ,Chromosome Aberrations ,Integrative Medicine ,Chemotherapy ,Antibiotics, Antineoplastic ,business.industry ,Daunorubicin ,Cytarabine ,Myeloid leukemia ,General Medicine ,Middle Aged ,Prognosis ,Antineoplastic Agents, Phytogenic ,Clinical trial ,Leukemia, Myeloid, Acute ,Treatment Outcome ,Complementary and alternative medicine ,Medicine public health ,Immunology ,Female ,Integrative medicine ,Mitoxantrone ,business ,Drugs, Chinese Herbal - Abstract
To observe the clinical efficacy of Chinese drugs combined with chemotherapy in the treatment of acute myeloid leukemia (AML) and to investigate the prognostic relevance of the main parameters in AML treated with integrative medicine.Forty AML patients hospitalized at the authors hospital were treated with Chinese drugs and chemotherapy. The routine examination, immunophenotype and karyotype analyses were carried out. The clinical efficacy was observed and the prognostic factors were analyzed.(1) Clinical efficacy: Twenty patients had complete remission (CR), with the CR rate being 50.0%. Among these patients, the CR rate was 73.9% (17/23) in de novo AML and 17.6% (3/17) in secondary or refractory AML, respectively. The median disease free survival (DFS) was 6 months (2-32 months) and median overall survival (OS) was 7 months (1-36 months). (2) Analysis of prognostic factors: Aging (60 years) and hepatosplenomegaly or extramedullary leukemia did not affect the treatment outcome. Patients with lower white blood cell (WBC) counts (4.0x10(9)/L) had a significantly higher CR rate (P0.01). Secondary or refractory AML was associated with a lower CR rate and shorter OS (P0.01,P0.05). Expression of CD34 was an adverse factor for obtaining CR (P0.05) and survival in both DFS and OS (P0.05,P0.01). The expression of CD56 was significantly associated with a lower CR rate (P0.05), but did not affect DFS and OS. Twenty-three (57.5%) out of 40 cases had chromosomal abnormalities. The CR rate was decreased and both DFS and OS shortened stepwise from the cases with favorable cytogenetics to those with intermediate and unfavorable cytogenetics (P0.01).The combined treatment of Chinese drugs with chemotherapy has a predominant effect in de novo AML. Secondary or refractory AML, expression of CD34 and CD56, and unfavorable cytogenetics were the main factors of poor prognosis in AML.
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- 2009
43. Molecular epidemiological characterization and health burden of thalassemia in Jiangxi Province, P. R. China
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Xin Tong, Fen Lin, Min Lin, Li-Ye Yang, Hui-Tian Yang, Xiao-Fen Zhan, Jiao-Ren Wu, Rong Hu, Xiao-Mei Hu, Wen-Yu Li, Hui Yang, Jian-Zhong Wang, Tianyu Zhong, Zhao-Yun Luo, and Yi-Guo Chen
- Subjects
Male ,Health Screening ,Spatial Epidemiology ,Epidemiology ,Thalassemia ,lcsh:Medicine ,Alpha-thalassemia ,Pediatrics ,Database and Informatics Methods ,Cost of Illness ,Animal Cells ,hemic and lymphatic diseases ,Molecular Cell Biology ,Medicine and Health Sciences ,Prevalence ,Medicine ,Public and Occupational Health ,lcsh:Science ,Genetics ,Molecular Epidemiology ,Multidisciplinary ,Obstetrics ,Child and Adolescent Health Policy ,Microcytosis ,Data Collection ,Child Health ,Beta thalassemia ,Hematology ,Middle Aged ,Healthy Volunteers ,3. Good health ,Health Education and Awareness ,Genetic Epidemiology ,Alpha-Thalassemia ,Epidemiological Methods and Statistics ,Female ,Cellular Types ,Genetic Dominance ,Research Article ,Adult ,medicine.medical_specialty ,China ,congenital, hereditary, and neonatal diseases and abnormalities ,Beta-Thalassemia ,Adolescent ,Prenatal diagnosis ,Health Informatics ,Research and Analysis Methods ,Young Adult ,Autosomal Recessive Diseases ,Hydrops fetalis ,parasitic diseases ,Humans ,Aged ,Blood Cells ,Autosomal Recessive Traits ,Health Care Policy ,Molecular epidemiology ,Population Biology ,business.industry ,lcsh:R ,Biology and Life Sciences ,Health Risk Analysis ,Human Genetics ,Cell Biology ,medicine.disease ,Blood Cell Count ,Health Care ,Hemoglobinopathies ,Mutation ,lcsh:Q ,business - Abstract
Background Thalassemia is the most common inherited disease in southern China. However, this disorder is usually ignored by Jiangxi provincial health system and government due to lack of epidemiological data. Materials and Methods A total of 9489 samples from Hakka Han and Gan-speaking Han in three geographical areas of Jiangxi Province were analyzed for both complete blood cell (CBC) count and reverse dot blot (RDB) gene chip for thalassemia. Results 1182 cases of suspected thalassemia carriers with microcytosis (MCV
- Published
- 2014
44. Effect of fuzheng kangbai granule on immune function and survival time in minimal residual leukemia model mice
- Author
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Yong-gang Xu, Rou Ma, Nai-ping Hu, Feng Liu, Jing-min Yang, Ling Ma, and Xiao-mei Hu
- Subjects
Leukemia ,Immune system ,animal diseases ,Medicine public health ,Granule (cell biology) ,Immunology ,medicine ,General Medicine ,Biology ,medicine.disease ,Residual - Abstract
Objective To observe the effects of Fuzheng Kangbai Granule (FZKBG) on immune function and survival time in minimal residual leukemia (MRL) model mice and explore its mechanism.
- Published
- 2001
45. Present status of study on reversion of anti-leukemic multidrug resistance
- Author
-
Rou Ma, Deng Chengshan, and Xiao-mei Hu
- Subjects
Multiple drug resistance ,business.industry ,Medicine public health ,Reversion ,Cancer research ,Medicine ,General Medicine ,business - Published
- 2001
46. Functional ionic liquids for hydrolysis of lignocellulose
- Author
-
Bao-Zhong Hu, Xiao-Mei Hu, Bixian Zhang, Yang Zhao, Kun Niu, and Yibo Xiao
- Subjects
chemistry.chemical_classification ,Reaction conditions ,Polymers and Plastics ,Organic Chemistry ,food and beverages ,Straw ,Chloride ,Reducing sugar ,Catalysis ,Hydrolysis ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Yield (chemistry) ,Ionic liquid ,Materials Chemistry ,medicine ,Nuclear chemistry ,medicine.drug - Abstract
An efficient system for hydrolysis of lignocellulosic materials to prepare reducing sugar in a series of functional acidic ionic liquids with low synthetic cost and excellent dissolved and catalytic activity was established. High yield of reducing sugar was obtained with the use of 1-H-3-methylimidazolium chloride ([HMIM]Cl). The use of ionic liquid under ultrasound irradiation greatly improved the yield of total reducing sugar. The optimum reaction conditions were as follows: ratio of water/sample was 5 (w/w), ratio of IL/sample was 25 (w/w), 70 °C, 120 min and the yield of reducing sugar was up to 53.27 mg from 0.2 g of soybean straw and 50.03 mg from 0.2 g of corn straw.
- Published
- 2013
47. [Clinical observation of Zhuhuang Decoction in ovulation induction]
- Author
-
Xiao-Mei Hu, Jin-Rong Fu, Jin Zheng, Xiang-Yun Li, and Hang Cheng
- Subjects
Adult ,medicine.medical_treatment ,Treatment outcome ,Physiology ,Decoction ,law.invention ,Anovulation ,Ovulation Induction ,law ,medicine ,Humans ,Estradiol ,business.industry ,Female infertility ,Luteinizing Hormone ,medicine.disease ,Treatment Outcome ,Complementary and alternative medicine ,Ovulation induction ,Female ,Phytotherapy ,business ,Luteinizing hormone ,Infertility, Female ,Drugs, Chinese Herbal - Published
- 2006
48. Digital Gene Expression Analysis of Corky Split Vein Caused by Boron Deficiency in ‘Newhall’ Navel Orange (Citrus sinensis Osbeck) for Selecting Differentially Expressed Genes Related to Vascular Hypertrophy
- Author
-
Lina Fu, Ji-Cui An, Shu-Ang Peng, Tingshuai Yan, Xiao Liu, Qingjiang Wei, Nannan Wang, Yong-Zhong Liu, Hui-Qing Yan, Xiao-Mei Hu, Gaofeng Zhou, Long-Fei Jin, Cheng-Quan Yang, and Shuang Li
- Subjects
Science ,Gene Expression ,Crops ,Plant Science ,Biology ,Genes, Plant ,Real-Time Polymerase Chain Reaction ,Fruits ,Molecular Genetics ,Transcriptome ,chemistry.chemical_compound ,Gene Expression Regulation, Plant ,Gene expression ,Gene ,Boron ,Gene Library ,Plant Diseases ,Plant Growth and Development ,Regulation of gene expression ,Genetics ,Multidisciplinary ,Gene Expression Profiling ,Computational Biology ,Agriculture ,Genomics ,Plants ,Cell Cycle Gene ,Molecular biology ,Gene expression profiling ,Gene Ontology ,chemistry ,Cytokinin ,Medicine ,Plant Vascular Bundle ,Genome Expression Analysis ,Metabolic Networks and Pathways ,Citrus × sinensis ,Research Article ,Developmental Biology ,Citrus sinensis - Abstract
Corky split vein caused by boron (B) deficiency in 'Newhall' Navel Orange was studied in the present research. The boron-deficient citrus exhibited a symptom of corky split vein in mature leaves. Morphologic and anatomical surveys at four representative phases of corky split veins showed that the symptom was the result of vascular hypertrophy. Digital gene expression (DGE) analysis was performed based on the Illumina HiSeq™ 2000 platform, which was applied to analyze the gene expression profilings of corky split veins at four morphologic phases. Over 5.3 million clean reads per library were successfully mapped to the reference database and more than 22897 mapped genes per library were simultaneously obtained. Analysis of the differentially expressed genes (DEGs) revealed that the expressions of genes associated with cytokinin signal transduction, cell division, vascular development, lignin biosynthesis and photosynthesis in corky split veins were all affected. The expressions of WOL and ARR12 involved in the cytokinin signal transduction pathway were up-regulated at 1(st) phase of corky split vein development. Furthermore, the expressions of some cell cycle genes, CYCs and CDKB, and vascular development genes, WOX4 and VND7, were up-regulated at the following 2(nd) and 3(rd) phases. These findings indicated that the cytokinin signal transduction pathway may play a role in initiating symptom observed in our study.
- Published
- 2013
49. Nanomedicine Design Based on Abnormal Structure in Tumor
- Author
-
Yu-Ran Huang, Xing-Jie Liang, Xiao-Mei Hu, Yuan-Yuan Zhao, Xue Xue, Jingyu Yang, and Xiao-Xi Zhuang
- Subjects
business.industry ,Cancer research ,Medicine ,Nanomedicine ,Plant Science ,business ,Agronomy and Crop Science ,Abnormal structure ,Biotechnology - Published
- 2013
50. Effects of an EPSPS-transgenic soybean line ZUTS31 on root-associated bacterial communities during field growth.
- Author
-
Gui-Hua Lu, Cheng-Yi Tang, Xiao-Mei Hua, Jing Cheng, Gu-Hao Wang, Yin-Ling Zhu, Li-Ya Zhang, Hui-Xia Shou, Jin-Liang Qi, and Yong-Hua Yang
- Subjects
Medicine ,Science - Abstract
The increased worldwide commercial cultivation of transgenic crops during the past 20 years is accompanied with potential effects on the soil microbial communities, because many rhizosphere and endosphere bacteria play important roles in promoting plant health and growth. Previous studies reported that transgenic plants exert differential effects on soil microbial communities, especially rhizobacteria. Thus, this study compared the soybean root-associated bacterial communities between a 5-enolpyruvylshikimate-3-phosphate synthase -transgenic soybean line (ZUTS31 or simply Z31) and its recipient cultivar (Huachun3 or simply HC3) at the vegetative, flowering, and seed-filling stages. High-throughput sequencing of 16S rRNA gene (16S rDNA) V4 hypervariable region amplicons via Illumina MiSeq and real-time quantitative PCR (qPCR) were performed. Our results revealed no significant differences in the overall alpha diversity of root-associated bacterial communities at the three developmental stages and in the beta diversity of root-associated bacterial communities at the flowering stage between Z31 and HC3 under field growth. However, significant differences in the beta diversity of rhizosphere bacterial communities were found at the vegetative and seed-filling stages between the two groups. Furthermore, the results of next generation sequencing and qPCR showed that the relative abundances of root-associated main nitrogen-fixing bacterial genera, especially Bradyrhizobium in the roots, evidently changed from the flowering stage to the seed-filling stage. In conclusion, Z31 exerts transitory effects on the taxonomic diversity of rhizosphere bacterial communities at the vegetative and seed-filling stages compared to the control under field conditions. In addition, soybean developmental change evidently influences the main symbiotic nitrogen-fixing bacterial genera in the roots from the flowering stage to the seed-filling stage.
- Published
- 2018
- Full Text
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