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Made available in DSpace on 2021-06-25T11:13:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-01 The aim of the present study was to investigate the effect of shrinking and no shrinking dental filling materials combination in posterior restorations under the combined effects of polymerization shrinkage and occlusal load by means of 3D Finite Elements Analysis. Six computer-generated and restored class I or class II cavities models of a lower molar were designed in the CAD software and evaluated according to the cavity and restorative procedure. Different shrinking and no shrinking adhesive materials combination with diverse Young’s modulus were considered. A food bolus was modeled on the occlusal surface replicating the chewing load using static linear analyses Polymerization shrinkage was simulated for the shrinking different restorative materials. The maximum principal stress was selected as analysis criteria. All models exhibited higher stresses along the dentine restoration interfaces with different magnitude and a similar stress trend along enamel restoration interface. Stress values up to 22 MPa and 19 MPa were recorded in the enamel and restoration, respectively. The use of elastic not shrinking material layer in combination with bulk fill composite reduced the stress magnitude in dentine and enamel to replace dental tissues. Class I and class II posterior cavities adhesively restored with shrinking filling material’s combination showed the most unfavorable stress concentrations and the multilayer technique is a promising restorative alternative in posterior adhesive restorations when deep dentin and enamel volumes are missing. School of Dentistry University of Naples Federico II, via S. Pansini 5 Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University-UNESP Fraunhofer JL IDEAS Department of Industrial Engineering University of Naples Federico II Endodontic Clinical Section School of Dentistry Department of Biomedical and Neuromotor Sciences University of Bologna-Alma Mater Studiorum School of Dentistry University of Taubaté–UNITAU, Taubaté Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University-UNESP

Made available in DSpace on 2022-05-01T09:47:13Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-02 The aim of this study was to evaluate the effect of biologically oriented preparation technique on the stress concentration of endodontically treated upper central incisors restored with zirconia crown (yttria-stabilized zirconia polycrystalline ceramic) through finite element analysis (FEA). Four models of maxillary central incisors containing enamel, dentin, periodontal ligament, cortical and medullary bone were created in CAD. Each model received a polymeric core-build up with nanofilled dental resin composite. The evaluated models were SM—preparation in shoulder 90◦; CM—chamfer preparation; BOPT—biologically oriented preparation technique and BOPTB—BOPT preparation 1 mm below the cement-enamel junction. All models received zirconia crowns (5Y-TZP), fiberglass post and 1 mm ferrule. The models were imported into the analysis software with parameters for mechanical structural testing using the maximum principal stress and the tensile strength as the analysis criteria. Then, load of 150 N was applied at the cingulum with 45◦ slope to the long axis of the tooth, with the fixed base for each model. The type of marginal preparation affected the stresses concentration in endodontically treated teeth and in the zirconia crown margin. Considering the stress magnitude only, BOPT is a viable option for anterior monolithic zirconia crowns; however, with the highest stress magnitude at the restoration margin. Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (Unesp) Faculty of Dentistry Universidade Estadual do Oeste do Paraná (UNIOESTE) Department of Dentistry University of Taubaté (UNITAU) School of Dentistry University of Naples Federico II, via S. Pansini 5 Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (Unesp)

Made available in DSpace on 2021-06-25T11:04:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01 This in vitro study investigated whether Carbopol 980 polymer could potentiate the anti-erosive effect of solutions containing sodium fluoride (F) and sodium fluoride associated with stannous chloride (FS). The dissolution of hydroxyapatite treated with the experimental solutions (F [500 ppm F-], F + Carbopol [0.1%], FS [500 ppm F- + 800 ppm Sn2+], FS + Carbopol) was evaluated. Deionized water was the negative control, and a commercial mouth rinse (AmF/NaF/SnCl2; 500 ppm F + 800 ppm Sn2+; Elmex® Erosion Protection) was the positive control. The solutions were also evaluated in an erosion-rehardening protocol, with two treatments per day, using bovine enamel specimens (n = 15) and human saliva. The acid challenge was performed using 0.3% citric acid (pH 2.6) for 2 min. Microhardness was measured at different times: baseline, after the first erosive challenge, after treatment, and after the second erosive challenge. Based on microhardness values, the demineralization, rehardening, and protective potentials were calculated. The alkali-soluble fluoride on enamel surfaces was also measured. Data were analyzed using ANOVA and Tukey tests (α = 0.05). Groups treated with FS + Carbopol showed the lowest hydroxyapatite dissolution and the highest rehardening and protective potentials. The measurement of alkali-soluble fluoride on enamel surfaces was also higher in the FS + Carbopol group. Carbopol was able to significantly increase the protective effect of the fluoridated solutions in addition to optimizing the adsorption of fluoride on the enamel surface. Department of Restorative Dentistry Institute of Science and Technology São Paulo State University - UNESP Avenida Engenheiro Francisco José Longo, 777, Jardim São Dimas Department of Restorative Dentistry Dental School University of Taubaté Department of Restorative Dentistry São Paulo University - USP Department of Chemical Engineering Polytechnic School São Paulo University - USP Department of Restorative Dentistry Institute of Science and Technology São Paulo State University - UNESP Avenida Engenheiro Francisco José Longo, 777, Jardim São Dimas

Made available in DSpace on 2022-05-01T06:31:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Objective: Evaluate the effect of four preparation designs, two ceramic materials, and two occlusion contact types on the stress distribution of ceramic veneer in upper central incisor. Material and methods: 3D-models were performed in the modeling software containing enamel, dentin, pulp, periodontal ligament and a base of polyurethane resin. The designs were modeled and exported to the computer aided engineering software to perform the static structural analysis. For the mesh, a total of 155429 tetrahedron elements and 271683 nodes were used, after a 10% convergence test. Two materials, lithium disilicate and feldspathic ceramics, were simulated. A static load of 100 N on 45º was applied on the incisal and middle thirds of the palatal tooth region, guided by the occlusal plane. The base was constrained in all directions. The Maximum Principal Stress was the failure criteria chosen for the analysis. Results: The Finite Element Analysis showed that the most conservative designs presented less stress concentration on the ceramic veneer. However, the highest tensile stress concentrations were observed on lithium disilicate veneer with extend design, on the middle third. The type of occlusal contact presented different stress patterns among the preparation designs; the incisal contact showed higher stress concentration compared to middle third contact regardless the ceramic material. Conclusion: To perform a ceramic veneer in upper central incisor, the feldspathic ceramic presented promising results and should be recommended when the extended design was done. Regarding contact types, the incisal contact is more prone to failure regardless the ceramic and preparation design. São Paulo State University (UNESP) Institute of Science and Technology Department of Restorative Dentistry University of Taubaté (UNITAU) Department Dentistry São Paulo State University (UNESP) Institute of Science and Technology Department of Restorative Dentistry

Made available in DSpace on 2022-04-29T08:31:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-08-01 The loss of dental structure caused by endodontic treatment is responsible for a decrease in tooth resistance, which increases susceptibility to fracture. Therefore, it is important that minimally invasive treatments be performed to preserve the dental structure and increase the resistance to fracture of endodontically treated posterior teeth. To evaluate under axial loads, using the finite element method, the stress distribution in endodontically treated molars restored with both transfixed or vertical glass fiber posts (GFP) and resin composite. An endodontically treated molar 3D-model was analyzed using finite element analyses under four different conditions, class II resin composite (G1, control model), vertical glass fiber post (G2), transfixed glass fiber posts (G3) and vertical and transfixed glass fiber posts (G4). Ideal contacts were considered between restoration/resin composite and resin composite/tooth. An axial load (300 N) was applied to the occlusal surface. The resulting tensile stresses were calculated for the enamel and dentin tissue from five different viewports (occlusal, buccal, palatal, mesial and distal views). According to the stress maps, similar stress trends were observed, regardless of the glass fiber post treatment. In addition, for the G1 model (without GFP), a high-stress magnitude can be noticed in the proximal faces of enamel (7.7 to 14 MPa) and dentin (2.1 to 3.3 MPa) tissue. The use of transfixed glass fiber post is not indicated to reduce the stresses, under axial loads, in both enamel and dentin tissue in endodontically treated molar with a class II cavity. Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (Unesp), São José dos Campos Graduate Program in Dentistry Department of Dentistry University of Taubaté (UNITAU) Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (Unesp), São José dos Campos

Made available in DSpace on 2021-06-25T11:13:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-02 The aim of the present investigation was to calculate the stress distribution generated in the root dentine canal during mechanical rotation of five different NiTi endodontic instruments by means of a finite element analysis (FEA). Two conventional alloy NiTi instruments F360 25/04 and F6 Skytaper 25/06, in comparison to three heat treated alloys NiTI Hyflex CM 25/04, Protaper Next 25/06 and One Curve 25/06 were considered and analyzed. The instruments’ flexibility (reaction force) and geometrical features (cross section, conicity) were previously investigated. For each instrument, dentine root canals with two different elastic moduli(18 and 42 GPa) were simulated with defined apical ratios. Ten different CAD instrument models were created and their mechanical behaviors were analyzed by a 3D-FEA. Static structural analyses were performed with a non-failure condition, since a linear elastic behavior was assumed for all components. All the instruments generated a stress area concentration in correspondence to the root canal curvature at approx. 7 mm from the apex. The maximum values were found when instruments were analyzed in the highest elastic modulus dentine canal. Strain and von Mises stress patterns showed a higher concentration in the first part of curved radius of all the instruments. Conventional Ni-Ti endodontic instruments demonstrated higher stress magnitudes, regardless of the conicity of 4% and 6%, and they showed the highest von Mises stress values in sound, as well as in mineralized dentine canals. Heat-treated endodontic instruments with higher flexibility values showed a reduced stress concentration map. Hyflex CM 25/04 displayed the lowest von Mises stress values of, respectively, 35.73 and 44.30 GPa for sound and mineralized dentine. The mechanical behavior of all rotary endodontic instruments was influenced by the different elastic moduli and by the dentine canal rigidity. Endodontic Clinical Section Department of Biomedical and Neuromotor Sciences School of Dentistry University of Bologna-Alma Mater Studiorum University of Taubaté (UNITAU), Taubaté Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University—UNESP Department of Chemical Materials and Industrial Production Engineering University of Naples Federico II and the Center for Advanced Biomaterials for HealthCare@CRIB Istituto Italiano di Tecnologia School of Dentistry University of Naples Federico II Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University—UNESP

Made available in DSpace on 2021-06-25T11:13:27Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-01 The purpose of this study is to evaluate the effect of pulp chamber extension angles and filling material mechanical properties on the biomechanical response of a ceramic endocrown. A 3D model of maxillary molar that underwent endodontically treatment was exported to computer aided design software to conduct finite element analysis (FEA). The endocrown model was modified considering different pulp chamber extension angles (right angle; 6°, 12° and 18° of axial divergence). The solids were imported into the computer aided engineering software in Standard for the Exchange of Product Data (STEP) format. Nine different filling materials were simulated to seal the orifice of the root canal system under each endocrown restoration (resin composite, bulk-fill resin composite, alkasite, flowable resin composite, glass ionomer cement, autocured resin-reinforced glass ionomer cement, resin cement, bulk-fill flowable resin composite, zinc oxide cement), totaling 36 models. An axial load (300 N) was applied at the occlusal surface. Results were determined by colorimetric graphs of von-Misses stress (VMS) and Maximum Principal Stress (MPS) on tooth, cement layer, and endocrown restorations. VMS distribution showed a similar pattern between the models, with more stress at the load region for the right-angled endocrowns. The MPS showed that the endocrown intaglio surface and cement layer showed different mechanical responses with different filing materials and pulp chamber angles. The stress peaks plotted in the dispersion plot showed that the filling material stiffness is proportional to the stress magnitude in the endocrown, cement layer and tooth adhesive surface. In addition, the higher the pulp chamber preparation angle, the higher the stress peak in the restoration and tooth, and the lower the stress in the cement layer. Therefore, 6° and 12° pulp chamber angles showed more promising balance between the stresses of the adhesive interface structures. Under the conditions of this study, rigid filling materials were avoided to seal the orifice of root canal system when an endocrown restoration was planned as rehabilitation. In addition, the pulp chamber axial walls were prepared between 6° and 12° of divergence to balance the stress magnitude in the adhesive interface for this treatment modality. School of Dentistry University of Taubaté Department of Human Pathology in Adulthood and Childhood “G. Barresi” University Hospital “G. Martino” of Messina, Via Consolare Valeria 1 Institute of Science and Technology São Paulo State University (Unesp) São José dos Campos Department of Biomedical and Dental Sciences and Morphofunctional Imaging University of Messina Institute of Science and Technology São Paulo State University (Unesp) São José dos Campos

Made available in DSpace on 2022-04-29T08:34:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-02-01 Objective Polymeric framework represent an innovative approach for implant-supported dental prostheses. However, the mechanical response of ultra-high performance polymers as frameworks for full-arch prostheses under the all-on-four concept remains unclear. The present study applied finite element analysis to examine the behavior of polyetherketoneketone (PEKK) and polyetheretherketone (PEEK) prosthetic frameworks. Materials and Methods A three-dimensional maxillary model received four axially positioned morse-taper implants, over which a polymeric bar was simulated. The full-arch prosthesis was created from a previously reported database model, and the imported geometries were divided into a mesh composed of nodes and tetrahedral elements in the analysis software. The materials were assumed as isotropic, elastic, and homogeneous, and all contacts were considered bonded. A normal load (500 N magnitude) was applied at the occlusal surface of the first left molar after the model was fixed at the base of the cortical bone. The microstrain and von-Mises stress were selected as criteria for analysis. Results Similarities in the mechanical response were observed in both framework for the peri-implant tissue, as well as for stress generated in the implants (263-264 MPa) and abutments (274-273 MPa). The prosthetic screw and prosthetic base concentrated more stress with PEEK (211 and 58 MPa, respectively) than with PEKK (192 and 49 MPa), while the prosthetic framework showed the opposite behavior (59 MPa for PEEK and 67 MPa for PEKK). Conclusion The main differences related to the mechanical behavior of PEKK and PEEK frameworks for full-arch prostheses under the all-on-four concept were reflected in the prosthetic screw and the acrylic base. The superior shock absorbance of PEKK resulted in a lower stress concentration on the prosthetic screw and prosthetic base. This would clinically represent a lower fracture risk on the acrylic base and screw loosening. Conversely, lower stress concentration was observed on PEEK frameworks. Federal University of Espírito Santo Rede Nordeste de Biotecnologia, Espírito Santo Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University, São José dos Campos São Paulo Department of Dentistry University of Taubaté, 09 Operários Street, São Paulo Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University, São José dos Campos São Paulo

Made available in DSpace on 2021-06-25T11:15:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 This study evaluated the stress distribution in five different class II cavities of premolar models restored with conventional or bulk-fill flowable composite by means of finite element analysis (FEA) under shrinkage and occlusal loading. An upper validated premolar model was imported in the software, and five class II cavities with different occlusal extensions and dimensions were prepared: horizontal cavity on the mesial surface (horizontal slot), mesio-occlusal cavity, mesial cavity (vertical slot), tunnel type cavity and direct access cavity. The models were restored with conventional or bulk-fill flowable resin composite. The tested materials were considered as homogeneous, linear, and isotropic. The Maximum Principal Stress criteria was chosen to evaluate the tensile stress results. The lowest shrinkage stress value was observed in the direct access cavity restored with bulk-fill flowable resin composite (36.12 MPa). The same cavity, restored with conventional composite showed a score of 36.14 MPa. The horizontal slot cavity with bulk-fill flowable showed a score of 46.71 MPa. The mesio-occlusal cavity with bulk-fill flowable had a score of 53.10 MPa, while with conventional composite this was 55.35 MPa. Higher shrinkage stress was found in the vertical slot cavity with conventional resin 56.14 MPa, followed by the same cavity with bulk-fill flowable 56.08 MPa. Results indicated that the use of bulk-fill flowable composite resin more significantly decreased the polymerization shrinkage stress magnitude. The larger the cavity and the volume of material necessary to restore the tooth, the greater the residual stress on enamel and dentin tissue. Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (UNESP) Department of Dentistry University of Taubaté (UNITAU) Department of Oral and Maxillo Facial Sciences “Sapienza” University of Rome Department of Neurosciences Reproductive and Odontostomatological Sciences School of Dentistry University of Naples Federico II Department of Dental Materials and Prosthodontics Institute of Science and Technology São Paulo State University (UNESP)

(1) Background: Trauma is a very common experience in contact sports, however, there is an absence of data regarding the effect of athletes wearing mouthguards (MG) associated with ankylosed maxillary central incisor during a traumatic impact. (2) Methods: To evaluate the stress distribution in the bone and teeth in this situation, models of maxillary central incisor were created containing cortical bone, trabecular bone, soft tissue, root dentin, enamel, periodontal ligament, and antagonist teeth were modeled. One model received a MG with 4-mm thickness. Both models were subdivided into finite elements. The frictionless contacts were used and a nonlinear dynamic impact analysis was performed in which a rigid object hit the model at 1 m&middot, s&minus, 1. For each model, an ankylosed periodontal ligament was simulated totaling 4 different situations. The results were presented in von-Mises stress maps. (3) Results: A higher stress concentration in teeth and bone was observed for the model without a MG and with ankylosed tooth (19.5 and 37.3 MPa, respectively), the most promising mechanical response was calculated for patients with healthy periodontal ligament and MG in position (1.8 and 7.8 MPa, respectively). (4) Conclusions: The MG&rsquo, s use is beneficial for healthy and ankylosed teeth, since it acts by dampening the generated stresses in bone, dentin, enamel and periodontal ligament. However, patients with ankylosed tooth are more prone to root fracture even when the MG is in position compared to a healthy tooth.

Made available in DSpace on 2020-12-12T02:47:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-10-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: To evaluate if the bioadhesive polymer (Carbopol 980) could potentiate the protective effect of sodium fluoride with stannous chloride (FS) solution on the control of enamel erosive wear. Methods: Cylindrical bovine enamel specimens were polished and randomly allocated into three groups (n = 60): FS (500 ppm F- +800 ppm Sn2+ - positive control), FS + Carbopol (0.1% Carbopol), and ultrapure water (negative control). A randomized double-blind cross-over in situ model with three phases was used. In each phase, volunteers (n = 15) used a palatal appliance containing 4 specimens: two were submitted to an erosion model (2 h of pellicle formation; immersion in 1% citric acid, pH 2.3, for 5 min, 4x/day; 1 h intervals of saliva exposure; and treatment with the test solutions for 1 min, 2x/day). Besides erosion, the other two specimens were also subjected to abrasion (2x/day, 15 s) with active electric toothbrush, before the treatment with the solutions. After 5 days, enamel surface loss (μm) was evaluated by profilometry. Data were analyzed by two-way RM-ANOVA and Tukey tests (5%). Results: There were significant differences for both challenge and treatment factors. Erosion/abrasion challenge resulted in significantly higher enamel loss than erosion only (p < 0.05). The surface loss values for the erosion/remineralization model were (means ± SL): C = 14.7 ± 5.8b; FS = 9.0 ± 7.5ab; FS + Carbopol = 5.9 ± 3.8a; and for erosion/abrasion: C = 26.6 ± 10.1c; FS = 15.0 ± 8.8b; FS + Carbopol = 12.3 ± 7.9ab. Conclusion: The association of Carbopol to the FS solution significantly protected the enamel against erosive wear, but it was not significantly superior to FS only. Clinical Significance: Under highly erosive and abrasive conditions, rinsing with solutions containing sodium fluoride plus stannous chloride, associated or not with the Carbopol polymer, is an effective approach to control enamel erosive wear. Department of Restorative Dentistry Institute of Science and Technology São Paulo State University-UNESP Department of Restorative Dentistry Dental School University of Taubaté Department of Restorative Dentistry São Paulo University-USP Department of Chemical Engineering Polytechnic School São Paulo University-USP Department of Restorative Dentistry Institute of Science and Technology São Paulo State University-UNESP

Objectives The present study examined a technique for reducing dentin permeability through the application of a calcium phosphate (CaP)-based desensitiser with a laser-assisted process and evaluated adhesive-dentin bond strength. Methods: Thirty dentin discs were divided into two groups according to whether the selected desensitiser (TeethMate; Kuraray Noritake) was used prior to dentin bonding. Each group was subdivided into three subgroups (n = 5): A- Adhesive (Single Bond Universal, 3M ESPE), AL- Adhesive + Laser (Nd:YAG 60 mJ) and LAL- Laser + Adhesive + Laser. Dentin permeability values (%) were recorded before and after desensitiser application. Resin composites were placed over the bonded specimens; the latter were aged prior to microtensile bond strength evaluation. Gelatinolytic activity within the hybrid layers was examined with in-situ zymography using confocal laser scanning microscopy. Data were analysed with ANOVA and Tukey test (α = 0.05). Results Significant differences in dentin permeability were identified for all groups (p = 0.00). Both laser treatment (p = 0.182) and desensitiser application (p = 0.687) did not significantly improve dentin bond strength. Ultrastructure of the resin-dentin interface identified presence of calcium phosphate within dentinal tubules. Laser treatment did not affect hybrid layer ultrastructure. Both treatment modalities (intratubular CaP occlusion and laser) had no influence on gelatinolytic activity within hybrid layers. Conclusion Although intratubular CaP occlusion and laser treatment were effective in reducing dentin permeability, they did not affect bond strength, interfacial ultrastructure and gelatinolytic activity within hybrid layers. Clinical relevance Treatment of etched dentin with Nd:YAG Laser at 60 mJ does not adversely affect collagen ultrastructure and gelatinolytic activity within the hybrid layer. The application of a calcium phosphate-based desensitiser to etch dentin does not affect dentin bond strength., Materials science; Biomedical engineering; Bond strength; Calcium phosphate; Dentin permeability; Nd:YAG laser.

Made available in DSpace on 2020-12-12T01:18:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-01 Background: Inflammatory/immunological serum markers are useful for the early detection of organ dysfunction, helping the diagnosis of sepsis. Although the detection of blood biomarkers is a standard practice, the use of noninvasive samples (eg saliva) would be beneficial. Aim: To investigate the saliva of hospitalized patients with and without sepsis and identify the levels of inflammatory markers such as C-reactive protein (CRP), procalcitonin (PCT), interleukin 6 (IL-6) and nitric oxide (NO). Methods: Saliva samples were collected from 26 patients in intensive care unit with diagnosis of sepsis and from 26 without sepsis (control). The levels of CRP were determined by using latex agglutination test, whereas those of procalcitonin and IL-6 by ELISA and NO by the Griess reaction. Results: Of 26 patients with sepsis, 14 were males (54%) with a mean age of 63.81 ± 3.48 years. The control group had the same distribution for gender, with mean age 65.04 ± 4.07 years. Sepsis group showed higher salivary concentrations of CRP, PCT, IL-6 and NO, with only levels of IL-6 being statistically different (P =.0001). Conclusions: Patients with sepsis had significantly higher levels of IL-6 in their saliva, suggesting that this biological sample could be useful in the diagnosis of this condition. UNITAU - University of Taubaté UNESP – State of São Paulo University UNISA - University of Santo Amaro HUMANITAS - School of Medical Sciences of São José dos Campos UNESP – State of São Paulo University

Made available in DSpace on 2021-06-25T10:13:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 There is an increased effort on developing novel and active surfaces in order to accelerate their osteointegration, such as nanosized crystalline hydroxyapatite coating (HAnano®). To better understand the biological behavior of osteoblasts grown on HAnano® surface, the set of data was compared with SLActive®, a hydrophilic sandblasted titanium surface. Methodologically, osteoblasts were seeded on both surfaces up to 72 hours, to allow evaluating cell adhesion, viability, and set of genes encoding proteins related with adhesion, proliferation, and differentiation. Our data shows HAnano® displays an interesting substrate to support cell adhesion with typical spread morphologic cells, while SLActive®-adhering cells presented fusiform morphology. Our data shows that the cellular adhesion mechanism was accompanied with upexpression of integrin β1, Fak, and Src, favoring the assembling of focal adhesion platforms and coupling cell cycle progression (upmodulating of Cdk2, Cdk4, and Cdk6 genes) in response to HAnano®. Additionally, both bioactive surfaces promoted osteoblast differentiation stimulus, by activating Runx2, Osterix, and Alp genes. Although both surfaces promoted Rankl gene expression, Opg gene expression was higher in SLActive® and this difference reflected on the Rankl/Opg ratio. Finally, Caspase1 gene was significantly upmodulated in response to HAnano® and it suggests an involvement of the inflammasome complex. Collectively, this study provides enough evidences to support that the nanohydroxyapatite-coated surface provides the necessary microenvironment to drive osteoblast performance on dental implants and these stages of osteogenesis are expected during the early stages of osseointegration. Lab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP-Saõ Paulo State University School of Dentistry University of Taubaté Program in Environmental and Experimental Pathology Paulista University Lab. of Bioassays and Cellular Dynamics Department of Chemical and Biological Sciences Institute of Biosciences UNESP-Saõ Paulo State University

Made available in DSpace on 2019-08-28T18:55:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2019 This study evaluated the effect of the CAD/CAM burs experience (newer vs older as consequence of the milling sequence) on fatigue failure load (FFL), number of cycles for failure (CFF), and survival rates of lithium disilicate glass-ceramic simplified restorations adhesively cemented to a dentin analogue substrate. Three sets of CAD/CAM burs were used to mill disc-shaped ceramic specimens (1 bur set - 18 milled discs with 10 mm diameter and 1.5 mm thickness), considering the bur experience as a result of the milling sequence to compose the study groups: G1-6 - discs obtained from the 1st to 6th milling of each bur set; G7-12 - specimens from the 7th to 12th milling; G13-18 - discs from the 13th to 18th. Discs of dentin analogue (G10, 10 mm diameter and 2.0 mm thickness) were made to serve as substrate (base material) and randomly assigned into pairs with the respective ceramic discs. Then, the ceramic discs were adhesively cemented onto the dentin analogue substrate, composing a three-layer specimen that mimics a monolithic restoration of a posterior tooth. Specimens were tested under stepwise fatigue approach: frequency = 20 Hz, 5000 cycles at maximum load of 400 N to accommodate the testing assembly, followed by incremental steps of 200 N with initial load ranging from 10 to 1000 N, to a maximum of 20,000 cycles/each step, until the occurrence of failure (radial crack). FFL and CFF were recorded at the end of the testing and subjected to statistical analysis. Supplementary roughness analysis of the milled surface was performed (n = 18) using a contact profilometer. Residual stress after milling and acid etching were accessed via X-ray Diffractometry analysis. FFL and CFF were not affected by increase on bur experience (no statistical differences among groups), despite that, it affected both Ra and Rz parameters (G1-6 had the smoothest surface). The residual stress concentration was negligible (milling did not induce residual stress concentration). It is concluded that the fatigue behavior of adhesively cemented lithium-disilicate glass-ceramic restorations was not influenced by CAD/CAM bur experience (newer vs older as consequence of the milling sequence), and so the residual stress concentration induced by milling was negligible. [de Andrade, Guilherme Schmitt; Diniz, Vandeberg; Datte, Carlos Eduardo; Bottino, Marco Antonio; de Melo, Renata Marques] Sao Paulo State Univ Unesp, Inst Sci & Technol, Dept Dent Mat & Prosthodont, Sao Jose Dos Campos, Brazil [Venturini, Andressa Bonin; Valandro, Luiz Felipe] Fed Univ Santa Maria UFSM, Fac Odontol, Prosthodont Unit, Oral Sci, Floriano Peixoto 1184, BR-97015372 Santa Maria, RS, Brazil [Rocha Pereira, Gabriel Kalil] Merid Fac IMED, Sch Dent, Passo Fundo, RS, Brazil [Amaral, Marina] Universidade de Taubaté (Unitau) , Dept Dent Prosthet Dent [Bastos Campos, Tiago Moreira] Aeronaut Technol Inst ITA, Dept Phys, Sao Jose Dos Campos, Brazil

This clinical study was conducted to correlate the levels of endotoxins and culturable bacteria found in primary endodontic infection (PEI) with the volume of root canal determined by using Cone Beam Computed Tomography (CBCT); and to evaluate the bacterial diversity correlating with clinical features. Twenty patients with PEI were selected and clinical features were recorded. The volume (mm3) of root canal was determined by CBCT analysis. Root canal samples were analyzed by using kinetic LAL-assay test to determine the levels of endotoxins and anaerobic technique to determine the bacterial count (CFU/mL). DNA was extracted from all samples to determine bacterial diversity and quantified by using Checkerboard-DNA-DNA- Hybridization. Culturable bacteria and endotoxins were detected in 100% of the root canal samples. Linear regression analysis revealed a correlation between root canal volume and presence of anaerobic bacteria (p

Made available in DSpace on 2022-04-29T08:28:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Aim: In dental implant treatment, there is a demand for mechanically stronger implants. Despite the existence of several studies showing the clinical success of narrow diameter implants, most of them are based on pure titanium (cpTi) alloys. There is a few clinical evidences of the success rate of titanium-zirconium (TiZr) narrow diameter implants. The aim of this study was to evaluate the stress distribution in the peri-implant area of narrow diameter cpTi and TiZr implants under axial and oblique loads. Methods: Photoelastic models were produced using epoxy resin (PL2, Vishay Precision Group) from a master model. The implants (cpTi and TiZr; Straumann AG) had 3.3 mm in diameter and 12 mm in height. Loads of 100 N and 200 N were applied to the abutment at angles of 0° (axial), 10°, 20°, and 30° (oblique). A circular polariscope (Eikonal) was used under dark field white-light configuration. The isochromatic fringes were analyzed in the peri-implant region in 5 areas, using ASTM table with isochromatic fringes; cervical-mesial, cervical-distal, mid-mesial, mid-distal and apical. Results: In general, under axial and oblique loads, the stress in the TiZr implant was lower than in the cpTi implant. The load of 200 N produced the highest stress values in cpTi and TiZr implants. In both implants and loads, the fringes were located more in apical area at all angles evaluated. Conclusion: It can be concluded that for small implants, the load inclination and intensity change the pattern of stress distribution and the cpTi implant exhibited the highest peri-implant stress. Department of Prosthodontics School of Dentistry University of Taubaté Department of Materials and Technology School of Engineering of Guaratinguetá São Paulo State University Department of Materials and Technology School of Engineering of Guaratinguetá São Paulo State University

Made available in DSpace on 2020-12-12T01:20:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Background: This study evaluated the presence of a silica-nylon mesh and two cantilever lengths on the biomechanical behavior of complete-arch implant-supported prostheses. Material and Methods: Twenty-four (24) complete mandibular arch implant-supported prostheses were divided into 4 groups according to the presence of reinforcing mesh (with or without) and the cantilever length (molar-15 mm or premolar-5 mm). The specimens were submitted to strain gauge analysis (30-kgf, 10 s) at different points (molar and premolar). Three-dimensional models were created based on the in vitro specimens, and the results in the bone (microstrain), prostheses (tensile stress), implants and prosthetic screws (von-Mises stress) were evaluated using the finite element method (FEM). All materials were considered homogeneous, isotropic and linear. Strain gauge data were submitted to 3-way analysis of variance and the Tukey test (α=.05). FEM results were qualitatively analyzed using colorimetric graphs. Results: The microstrain magnitude for the prostheses with reinforcement was 519.91±359 and 583.33±661 without reinforcement (p=.001). The microstrain values for loading on the molar was 867.49±784 and on the premolar was 235.75±145. FEM corroborated with the in vitro findings for the bone behavior. The load application in the premolar showed reduced stress concentration, and a significant difference was observed between the presence or absence of the reinforcement for the prostheses. Conclusions: Silica-nylon mesh reduced the peri-implant microstrain and the prosthesis stress regardless of the cantilever extension. For temporary complete-arch implant-supported prostheses, the limitation of the cantilever to the premolar region improves the biomechanical response during load application. Department of Dental Materials and Proshodontics São Paulo State University (Unesp) Institute of Science and Technology Department of Dentistry (Prosthethic Dentistry) University of Taubaté (Unitau) Department of Prosthodontics Brazcubas Education Department of Dental Materials and Proshodontics São Paulo State University (Unesp) Institute of Science and Technology

Made available in DSpace on 2018-12-11T17:13:09Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Statement of problem: Complete-arch implant-supported prostheses without a framework have a high risk of failure: a straightforward and inexpensive reinforcement material, such as nylon mesh, could improve their longevity. Purpose: The purpose of this in vitro study was to evaluate a nylon-silica mesh compound on the fracture strength of acrylic resin and the fracture load of complete-arch implant-supported prostheses. Material and methods: Twenty-four complete mandibular arch implant-supported prostheses were divided into 2 groups according to cantilever length (molar and premolar) and subdivided into another 2 subgroups according to the presence or absence of reinforcing mesh. The specimens were submitted to a maximum load-to-fracture test in a universal testing machine, with a 100-N load cell, a 2 mm/min crosshead speed, and a spherical metal tip diameter of 4 mm at different points (molar and premolar). These were submitted to 1-way analysis of variance for repeated measurement and the post hoc Tukey multiple comparison test (α=.05). Results: The mean maximum load ±standard deviation for the molar group was 393.4 ±95.0 N with reinforcement and 305.4 ±76.3 N without reinforcement (P=.02); and for the premolar group was 1083.3 ±283.7 N with reinforcement and 605.3 ±90.5 N without reinforcement (P=.001). Conclusions: Reinforcement with nylon mesh increased the mean maximum load of implant-supported complete-arch prostheses at both cantilever lengths. The cantilever to the premolar (5 mm) presented the highest maximum load values to fracture. Postdoctoral Program Institute of Aeronautics Technology (ITA) São José dos Campos Dental Prostheses Department of Dentistry University of Taubaté (UNITAU) Associate Professor Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) Institute of Science and Technology São José dos Campos Researcher Engineering Applications Laboratory Institute of Aeronautics Technology (ITA) São José dos Campos Associate Professor Department of Dental Materials and Prosthodontics São Paulo State University (UNESP) Institute of Science and Technology São José dos Campos FAPESP: 2011/07334-6

Made available in DSpace on 2018-12-11T17:16:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-04-01 This study evaluated the prophylactic effects of the live or heat-killed probiotic strain Lactobacillus rhamnosus ATCC 7469 in Galleria mellonella, inoculated with Staphylococcusaureus or Escherichia coli. L. rhamnosus suspension was prepared and a part of it was autoclaved to obtain heat-killed lactobacilli. The larvae were inoculated of these suspensions and pathogenic. The survival of the larvae was observed during 7 days and after 24 h of inoculation haemocytes counted, melanization and nitric oxide production were analyzed. Larvae survival rate increased in the group inoculated with heat-killed L. rhamnosus, however, with no statistical difference. There was a significant increase in total haemocyte counts in all test groups. Haemolymph melanization and nitric oxide production were higher in the group inoculated with L. rhamnosus and infected with S. aureus. It was concluded that, in this model of infection, heat-killed L. rhamnosus ATCC 7469 promoted greater protection in Galleria mellonella infected with S. aureus or E. coli. Department of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Paulista State University/UNESP, Av, Engenheiro Francisco José Longo, 777-Jardim São Dimas Bioscience Basic Institute University of Taubaté, Rua Expedicionário Ernesto Pereira, 3 Department of Biosciences and Oral Diagnosis Laboratory of Microbiology and Immunology Institute of Science and Technology Paulista State University/UNESP, Av, Engenheiro Francisco José Longo, 777-Jardim São Dimas

Made available in DSpace on 2019-09-12T16:53:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2018 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This study aimed to investigate the influence of Lactobacillus rhamnosus intake on the development of candidiasis and cytokines release. Candida suspensions were inoculated into the oral cavity of experimentally immunosuppressed mice for candidiasis induction. The animals were divided into experimental groups: candidiasis with no probiotic intake (F), candidiasis with probiotic intake during Candida inoculation (FP), and candidiasis with probiotic intake 14 days before inoculation with Candida (FPP); and control groups: (C), (CP), and (CPP) without inducing candidiasis with probiotic intake in the same manner as groups F, FP, and FPP, respectively. After these periods, samples were collected from the oral cavity for yeast counts and, after euthanasia, the tongues of the animals were removed for histological analysis. Sera samples were also collected for analysis of IL-1 beta, TNF-alpha, INF-gamma, IL-12, IL-4, and IL-10. FP group showed lower Candida counts in the oral cavity, and the presence of Candida was almost not detected in FPP group. In tissues, the counts of fungi were significantly lower in FPP group, followed by FP. Groups that consumed probiotics also had lower histological and inflammatory infiltrates compared to F. Cytokines analysis demonstrated low concentrations of TNF-alpha, IL-12, IL-4, and IL-10 in all the groups, and no statistical difference between them. The production of IL-6 could be better detected, and the experimental groups that consumed the probiotic showed significant lower levels of this cytokine. The results suggest that L. rhamnosus intake, especially preventively, may avoid or decrease the development of candidiasis in immunosuppressed mice. This work adds scientific evidences that probiotics intake can avoid the development of candidiasis. [Pereira Leao, Mariella Vieira; Goncalves e Silva, Celia Regina; Ferreira dos Santos, Silvana Soleo] Universidade de Taubaté (Unitau), Biosci Basic Inst, Av Tiradentes,500 Bom Conselho, BR-12030180 Taubate, SP, Brazil [Aureliano Tavares, Talita Angelina; Junqueira, Juliana Campos; de Oliveira, Luciane Dias; Cardoso Jorge, Antonio Olavo] Univ Estadual Paulista, Inst Sci & Technol, Dept Biosci & Oral Diag, UNESP, Av Engn Francisco Jose Longo,777 Jardim Sao Dimas, BR-12245000 Sao Jose Dos Campos, SP, Brazil

Made available in DSpace on 2019-09-12T16:25:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 Desequilíbrios na microbiota residente podem promover o crescimento de microrganismos oportunistas, como as leveduras do gênero Candida, e o desenvolvimento de doenças, especialmente na população idosa. Este estudo investigou se o consumo do probiótico Yakult LB® (Lactobacillus casei e Bifidobacterium breve) era capaz de influenciar na resposta imune secretória anti-Candida e na presença destes microrganismos na cavidade bucal de 42 idosas saudáveis. Amostras de saliva foram coletadas antes e depois do consumo do probiótico por 30 dias, 3 vezes por semana. As amostras foram semeadas em Agar Saboraud Dextrose com cloranfenicol, as unidades formadoras de colônias (UFC/mL) foram contadas e as espécies de Candida foram identificadas. A análise de IgA anti-Candida foi realizada pela técnica ELISA. Os resultados demonstraram redução na prevalência de Candida (de 92,9% para 85,7%), na contagem de UFC/mL (p≤0,05) e no número de espécies não-albicans, depois do consumo do probiótico. As análises imunológicas mostraram um aumento significativo dos níveis de IgA anti-Candida (p≤0,05). Concluindo, as bactérias probióticas reduziram significantemente a quantidade de Candida na cavidade bucal dos idosos e aumentaram a resposta imune secretória específica para esta levedura, sugerindo a possibilidade de sua utilização no controle da candidose bucal. Imbalance in the resident microbiota may promote the growth of opportunistic microorganisms, such as yeasts of Candida genus and the development of diseases, especially in aged people. This study evaluated whether the consumption of the probiotic Yakult LB® (Lactobacillus casei and Bifidobacterium breve) was able to influence on the specific immunological response against Candida and on the presence of these yeasts in the oral cavity of 42 healthy aged individuals. Saliva samples were collected before and after the probiotic use for 30 days, 3 times a week. The samples were plated in Dextrose Saboraud Agar with chloramphenicol, the colony-forming units (CFU/mL) were counted and the Candida species were identified. Anti-Candida IgA analysis was conducted using the ELISA technique. ANOVA and Student's t-test were used for normally distributed data and the Wilcoxon test was used for data with non-normal distribution (α=0.05). The results showed a statistically significant reduction (p

Made available in DSpace on 2013-08-12T18:46:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Made available in DSpace on 2013-08-12T18:46:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Made available in DSpace on 2013-09-30T18:34:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Made available in DSpace on 2013-09-30T18:34:07Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:04:30Z No. of bitstreams: 0 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T14:04:30Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T14:04:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Made available in DSpace on 2014-05-20T14:04:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2011-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Simvastatin, a drug used to lower blood cholesterol, has been reported to have an anabolic effect on bone. The purpose of this study was to evaluate the influence of simvastatin and demineralized bovine bone matrix (DBBM) on the repair of rat calvarial defects. Defects of 5 mm were created in 64 rats, divided into four groups: no local treatment (control); treatment with DBBM (DBBM); treatment with a combination of simvastatin solution (2.2 mg/50 mu l) and DBBM (DBBMSIM-1); and treatment with simvastatin solution (0.5 mg/50 mu l) and DBBM (DBBMSIM-2). Animals were sacrificed on postoperative day 30 or 60, after which the calvariae were X-rayed and prepared for histomorphometric evaluation. The data were submitted to statistical analysis (p < 0.05). Xrays revealed that, on postoperative day 30, animals treated with a lower dose of simvastatin presented the lowest bone density, whereas on postoperative day 60 the use of simvastatin, regardless of the dose, resulted in lower density than that observed in control and DBBM group samples. Histomorphometric analysis revealed that, on postoperative day 30, both DBBM and DBBMSIM-1 had a negative impact on bone formation. on postoperative day 60, none of the combinations tested impaired bone repair. These results showed that the association between DBBM and simvastatin had a negative impact on bone repair. UNITAU Univ Taubate, Dept Dent, Taubate, SP, Brazil UNESP Univ Estadual Paulista, Sao Jose dos Campos Sch Dent, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, Brazil UNESP Univ Estadual Paulista, Sao Jose dos Campos Sch Dent, Dept Biosci & Oral Diag, Sao Jose Dos Campos, SP, Brazil FAPESP: 07/55508-8

Made available in DSpace on 2019-09-12T16:56:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2009 Objective: The aim of this study was to correlate the presence of Enterobacteriaceae, Pseudomonadaceae, Moraxellaceae and Xanthomonadaceae on the posterior dorsum of the human tongue with the presence of tongue coating, gender, age, smoking habit and denture use. Material and Methods: Bacteria were isolated from the posterior tongue dorsum of 100 individuals in MacConkey agar medium and were identified by the API 20E system (Biolab-Merieux). Results: 43% of the individuals, presented the target microorganisms on the tongue dorsum, with greater prevalence among individuals between 40 and 50 years of age (p=0.001) and non-smokers (p=0.0485). Conclusions: A higher prevalence of Enterobacteriaceae and Pseudomonadaceae was observed on the tongue dorsum of the individuals evaluated. There was no correlation between these species and the presence and thickness of tongue coating, gender and presence of dentures. [Conti, Simone] Universidade de Taubaté (Unitau), Program Dent [Ferreira dos Santos, Silvana Soleo] Universidade de Taubaté (Unitau), Dept Dent [Koga-Ito, Cristiane Yumi; Cardoso Jorge, Antonio Olavo] Sao Paulo State Univ, Sao Jose Campos Dent Sch, Sao Jose Dos Campos, SP, Brazil

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:24:35Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:30:38Z : No. of bitstreams: 1 2-s2.0-72449170012.pdf: 287974 bytes, checksum: ab4f6ae1e41809afbceef88136df0475 (MD5) Made available in DSpace on 2014-05-27T11:24:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-12-28 Alcohol consumption is a risk indicator for periodontal disease. The purpose of this study was to morphometrically evaluate the influence of alcohol consumption on alveolar bone level associated with ligature-induced periodontitis in rats. Thirty-six female rats (Wistar, 120 days-old) were randomly divided into three groups that received a daily administration of a water diet (control, n = 12), a 10% alcohol diet (10% ethanol, n = 12) or a 20% alcohol diet (20% ethanol, n = 12). Four weeks after the onset of the experiment, cotton ligatures were placed around the cervix of the upper right second molar in six rats. The other 6 rats in each group remained unligated. The rats were sacrificed four weeks after ligature placement. The maxillary bones were removed and alveolar bone loss was analyzed by measuring the distance between the cementoenamel junction and the alveolar bone crest at 2 buccal and 2 palatal sites on the upper right second molar. Analyses between the ligated and unligated groups showed that the presence of ligature induced alveolar bone loss (p < 0.05). Unligated groups showed no significant differences between each other (p > 0.05). In the ligated groups, rats receiving 20% ethanol showed significantly greater bone loss compared to control rats or rats receiving 10% ethanol. These results demonstrate that alcohol consumption may increase alveolar bone loss in female rats in a dosedependent manner. Department of Dentistry University of Pindamonhangaba, Pindamonhangaba, SP Department of Periodontology School of Dentistry University of Taubaté, Taubaté, SP Department of Restorative Dentistry School of Dentistry Federal University of Santa Maria, Santa Maria, RS Department of Bioscience and Oral Diagnosis School of Dentistry of São José dos Campos São Paulo State University, São José dos Campos, SP Department of Bioscience and Oral Diagnosis School of Dentistry of São José dos Campos São Paulo State University, São José dos Campos, SP

Made available in DSpace on 2022-04-28T20:48:12Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-01-01 The use of denture is known to increase the carriage of Candida in healthy patients, and the proliferation of Candida albicans strains can be associated with denture-induced stomatitis. The aim of this study was to evaluate the use of vinegar as an antimicrobial agent for control of Candida spp. in complete upper denture wearers. Fifty-five patients were submitted to a detailed clinical interview and oral clinical examination, and were instructed to keep their dentures immersed in a 10% vinegar solution (pH less than 3) overnight for 45 days. Before and after the experimental period, saliva samples were collected for detection of Candida, counting of cfu/mL and identification of species by phenotypical tests (germ tube formation, chlamidoeonidia production, and carbohydrate fermentation and assimilation). The results were analyzed using Spearman's correlation and Student's t-test (p

Considerando que os tumores cerebrais correspondem à segunda neoplasia mais freqüente na infância, sendo apenas superados pelas leucemias, realizamos um estudo clínico prospectivo em setenta pacientes com tumor cerebral, com idade variando de um a quinze anos, por um período de quatro anos (1993-1997), através de acompanhamento ambulatorial. Avaliamos quarenta e um pacientes do sexo masculino e vinte e nove do sexo feminino. Observamos uma freqüência discretamente maior de tumores infratentoriais (38 casos) em relação aos de localização supratentorial (32 casos). O tumor mais freqüentimente observado foi o meduloblastoma (21 casos), seguido pelo astrocitoma (15 casos) e pelo germinoma (11 casos). No acompanhamento ambulatorial, observamos que 75,5% dos pacientes evoluíram com sequelas neurológicas. Em 34,3% houve recidiva tumoral e 21,4% dos pacientes evoluíram para óbito. Taken as proved that brain tumors are the second most frequent childhood neoplasm - only outnumbered by leukemias - we have undertaken a clinical perspective study with seventy brain tumor patients ranging from one to fifteen years of age, throughout a four-year period (1993-1997), based on ambulatory-oriented follow-up. Forty-one male and twenty-nine female patients were analyzed, in that a slightly higher number of infratentorial tumors was observed (thirty-eight cases), compared to those supratentorially located (thirty-two cases). The most repeatedly observed during the study was the medulloblastoma (twenty-one patients), followed by the astrocytoma (fifteen patients) and the germinoma (eleven patients). It should be pointed out that during the ambulatory follow-up 75,5% of patients developed neurological sequels. A tumor recurrence was noticed in 34,3% of them, while 21,4% eventually died. Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina University of Taubaté Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Department of Neurology and Neurosurgery Universidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Pediatric Neuro-oncology Division UNIFESP, EPM, Department of Neurology and Neurosurgery UNIFESP, EPM, Pediatric Neuro-oncology Division SciELO

Made available in DSpace on 2019-09-12T16:53:50Z (GMT). No. of bitstreams: 0 Previous issue date: 2012 The aim of the study was to assess the tensions generated in the long axis of the implants and the interimplants in the cervical, middle, and apical regions when subjected to different loads with or without interposition of the flat occlusal plane splint. A photoelastic model was made with 2 external hexagon implants located in the space corresponding to the second premolar and molar inferiors. A screw-retained metal superstructure was installed on the implants with a torque of 20 N.cm, and the set (photoelastic model with superstructure) were positioned in the circular polariscope in the dark-field configuration to observe the distribution of isochromatic fringes around the implants and interimplant areas. Photographic records were obtained before the application of the occlusal load with the following loading conditions: 300, 600, and 900 N, with and without interposition of the occlusal plane splint. The decrease in stress with the application of the flat occlusal plane splint became more evident after the application of the 600-N load. Generally, the major stress magnitude occurred in the cervical region for interimplant areas and in the apical region around implants. Use of occlusal splint in the loading of 300, 600, and 900 N reduced tension 33.22%, 66.66%, and 73.33%, respectively, in the samples. It can be concluded that the interposition of the occlusal plane splint caused a reduction in tension resulting from the simulation of occlusal overload. [Teixeira, Fabiola Marchezini; de Assis Claro, Cristiane Aparecida; Claro Neves, Ana Christina; da Silva-Concilio, Lais Regiane] Universidade de Taubaté (Unitau), Dept Prosthodont, Grad Program Dent, Sao Paulo, Brazil [Rode, Sigmar de Mello] Univ State Sao Paulo, Dept Dent Mat, Sao Jose dos Campos Dent Coll, Sao Paulo, Brazil

Sjögren's syndrome is an autoimmune disease characterized by sialoadenitis and elevated titers of autoantibodies. To assess whether it is possible to induce inflammatory changes in salivary gland tissues, a series of immunizations in Balb/c mice have been undertaken, using salivary gland extract, modified or not, added to several adjuvants. Mice's humoral immune response to salivary gland antigens was monitored by ELISA. Inflammatory cells infiltrating gland tissue were seen 3 months after immunization with salivary gland extract modified with pepsin (AgGp) and metaperiodate (AgGMp). Although pathological progression was not observed, the histopathological picture was similar to the initial phase of Sjögren's syndrome. In addition, a monoclonal antibody reactive with 3 gland polypeptides and anhydrase carbonic II was rescued among B cells from immunized mice. Thus, immunizations with modified autoantigens were able to initiate pathological damage to glandular tissue and stimulate the proliferation of auto-reactive B cells. A Síndrome de Sjögren é uma doença auto-imune caracterizada por desenvolvimento de sialoadenite e títulos elevados de auto-anticorpos. Com o objetivo de induzir alterações inflamatórias no tecido das glândulas salivares foram realizadas várias imunizações em camundongos BALB/c utilizando extratos de glândulas salivares, modificados ou não, em vários adjuvantes. A resposta humoral para antígenos salivares foi monitorada por ELISA. Células inflamatórias infiltrando o tecido glandular foram vistas 3 meses pós-imunização com extrato de glândula salivar modificado com pepsina (AgGp) e metaperiodato (AgGMp). Embora a evolução patológica não tenha sido observada, o quadro histopatológico foi semelhante à fase inicial da Síndrome de Sjögren. Também foi possível notar, a partir das células B dos animais imunizados, a produção de anticorpos monoclonais reativos com 3 polipeptídeos glandulares e anidrase carbônica II. Assim, a imunização com auto-antígenos glandulares modificados foi capaz de iniciar o processo patológico no tecido glandular e induzir a proliferação de células B produtoras de auto-anticorpos.

OBJECTIVE: The aim of this study was to evaluate the effect of the alcohol consumption on the periodontal bone support (PBS) in experimental periodontitis in rats. MATERIALS AND METHODS: Sixty-three male rats were divided into seven groups: G1 (control); G2 (10% ethanol); G3 (nutritional control of G2); G4 (20% ethanol); G5 (nutritional control of G4); G6 (30% ethanol) and G7 (nutritional control of G6). The groups G3, G5 and G7 received controlled diets with equivalent caloric amounts to those consumed in G2, G4 and G6 respectively, with the ethanol replaced by sucrose. After anesthesia, ligatures were installed around the mandibular first molar, leaving the contralateral teeth unligated. After 8 weeks, the rats were killed and their mandibles were radiographed to measure the percentage of PBS on the distal aspect. RESULTS: The intragroup analyses showed that presence of ligatures induced periodontitis (p

Made available in DSpace on 2021-07-14T10:50:28Z (GMT). No. of bitstreams: 0 Previous issue date: 2004-09. Added 1 bitstream(s) on 2021-07-14T11:39:21Z : No. of bitstreams: 1 S1806-83242004000300005.pdf: 229567 bytes, checksum: cc5a7ccb7ce664f4744cd3f68c7e0947 (MD5) O objetivo deste estudo foi analisar o efeito da sialoadenectomia sobre o desenvolvimento da candidose oral após uma ou quatro inoculações de Candida albicans. Inicialmente, uma suspensão contendo 10(8) células/ml de C. albicans ATCC 36801 foi preparada. Setenta e oito camundongos sialoadenectomizados e mesma quantidade de camundongos com fluxo salivar normal receberam uma única inoculação de suspensão de C. albicans. Outro grupo, com o mesmo número de camundongos, recebeu 4 inoculações. O grupo controle consistiu de 6 camundongos sialoadenectomizados e 6 com fluxo salivar normal que não foram inoculados com C. albicans. O desenvolvimento de candidose foi estudado histologicamente na língua dos animais em períodos de 1, 2, 3, 5 e 8 dias após a inoculação e em intervalos de 15 dias até 165 dias. De acordo com os resultados obtidos, conclui-se que a sialoadenectomia e uma maior freqüência de inoculação influenciaram na presença e extensão das lesões de candidose. The aim of this study was to analyze the effect of sialoadenectomy on the development of oral candidosis after one or four inoculations of Candida albicans. Initially, a suspension containing 10(8) cells/ml of C. albicans ATCC 36801 was prepared. Seventy-eight sialoadenectomized mice and a similar amount of mice with normal salivary flow received a single inoculation of C. albicans suspension. Another group with a similar number of mice received 4 inoculations. The control group consisted of 6 sialoadenectomized mice and 6 mice with normal salivary flow that were not inoculated with C. albicans. Candidosis development was studied histologically in the tongue of the animals 1, 2, 3, 5, and 8 days after inoculation and at 15-day intervals up to 165 days. According to the results obtained, it could be concluded that sialoadenectomy and a higher frequency of yeast inoculation influenced the presence and extension of candidosis lesions. Universidade Estadual de Campinas, School of Dentistry of Piracicaba University of Ponta Grossa, Department of Dentistry Universidade Estadual Paulista, School of Dentistry of São José dos Campos University of Taubaté, Department of Dentistry Universidade Estadual Paulista, School of Dentistry of São José dos Campos

Made available in DSpace on 2021-07-14T10:48:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-12. Added 1 bitstream(s) on 2021-07-14T11:38:43Z : No. of bitstreams: 1 S1517-74912003000400007.pdf: 248920 bytes, checksum: 867f6c7cc6b5dfe2515bb0d0d83cf7c5 (MD5) O efeito da adequação de meio sobre contagens de Candida spp. na cavidade bucal tem sido pouco discutido e nenhum resultado conclusivo foi encontrado na literatura. Desse modo, o objetivo do presente trabalho foi verificar os efeitos da adequação de meio bucal, utilizando cimento de óxido de zinco e eugenol e cimento de ionômero de vidro, sobre contagens desses microorganismos em crianças. Foram coletados enxágües bucais de 30 meninos e 30 meninas com idades entre quatro e dez anos, positivos para Candida na saliva, obtendo-se a contagem inicial. As crianças foram divididas ao acaso em dois grupos e procedeu-se à adequação de meio utilizando um dos cimentos a serem testados. Uma semana depois, novo enxágüe bucal foi coletado, obtendo-se, dessa forma, a contagem final de Candida spp. Uma redução expressiva nas contagens foi observada em ambos os grupos. Os dois materiais testados foram eficazes na diminuição das contagens de Candida, e diferenças estatisticamente significantes foram observadas entre as contagens inicial e final nos dois grupos. Considerando o percentual de redução, o cimento de óxido de zinco e eugenol apresentou resultados mais favoráveis, promovendo uma redução de 70%, ao passo que para o cimento de ionômero de vidro esse valor foi de 46%. De acordo com os resultados obtidos, concluiu-se que a adequação de meio é um procedimento eficaz na redução das contagens de Candida spp. na cavidade bucal, especialmente quando o cimento de óxido de zinco e eugenol é utilizado. The effects of oral environment stabilization procedures on counts of Candida spp. have rarely been discussed, and no conclusive results are found in the literature. The aim of this study was thus to ascertain the effects of oral environment stabilization procedures with glass ionomer and zinc oxide-eugenol cements on counts of Candida spp. in the oral cavity of children. For this purpose, oral rinses of sterile phosphate-buffered saline were initially collected from 30 boys and 30 girls, positive for Candida in the saliva and aged from 4 to 10 years. Data on the initial quantity of CFU/ml of Candida were obtained. Then, the children were randomly divided into two groups and oral environment stabilization procedures were performed using zinc oxide-eugenol cement or glass ionomer cement. One week after the procedures were performed, oral rinses were collected again and final Candida counts were obtained. An expressive reduction in Candida counts was observed in both groups. The zinc oxide-eugenol and glass ionomer cements were efficient in the reduction of Candida counts and statistically significant differences were observed between initial and final counts in both groups. Considering the percentage of reduction, the zinc oxide-eugenol cement presented more favorable results, with a reduction of 70%. A reduction of 46% was observed with the use of the glass ionomer cement. According to the obtained results, we concluded that oral environment stabilization procedures were efficient in reducing Candida spp. counts, especially when the zinc oxide-eugenol cement was employed. Universidade Estadual Paulista, School of Dentistry of São José dos Campos University of Taubaté, Department of Dentistry Universidade do Vale do Paraíba, Department of Pediatric Dentistry Universidade Estadual Paulista, School of Dentistry of São José dos Campos

Made available in DSpace on 2021-07-14T10:48:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-06. Added 1 bitstream(s) on 2021-07-14T11:38:43Z : No. of bitstreams: 1 S1517-74912003000200016.pdf: 129290 bytes, checksum: 46a4a89815dea079ef03f222f2c12885 (MD5) O presente estudo avaliou a prevalência de Actinobacillus actinomycetemcomitans de máxima e mínima leucotoxicidade em indivíduos com doença periodontal. Foram analisadas amostras de placa bacteriana subgengival de 136 indivíduos, entre 14 e 76 anos de idade, com algum tipo de doença periodontal. Os parâmetros clínicos avaliados incluíram profundidade de sondagem (PS), índice de placa bacteriana (IPB) e índice de sangramento gengival (ISG). A presença de amostras de A. actinomycetemcomitans de máxima e mínima leucotoxicidade foi avaliada por reação em cadeia da polimerase (PCR). Os resultados foram analisados empregando-se teste qui-quadrado e análise de regressão logística. Quarenta e sete indivíduos apresentaram gengivite, 70 indivíduos periodontite crônica e 19 indivíduos periodontite agressiva. O teste qui-quadrado não demonstrou correlação significativa entre PS (chi2 = 0,73), IPB (chi2 = 0,35), ISG (chi2 = 0,09) e a presença de amostras de máxima leucotoxicidade. A. actinomycetemcomitans de máxima leucotoxicidade apresentou associação com indivíduos com idade inferior a 28 anos (chi2 = 22,06). Os resultados observados nessa população brasileira confirmam a forte associação existente entre amostras de A. actinomycetemcomitans de máxima leucotoxicidade e periodontite agressiva. This study examined the prevalence of highly and minimally leukotoxic Actinobacillus actinomycetemcomitans in patients with periodontal disease. Pooled subgingival plaque samples from 136 patients with some form of periodontal disease were examined. Subjects were between 14 and 76 years of age. Clinical examinations included periodontal pocket depth (PD), plaque index (PI) and bleeding index (BI). The obtained plaque samples were examined for the presence of highly or minimally leukotoxic A. actinomycetemcomitans strains by the polymerase chain reaction (PCR). Chi-square and logistic regression were performed to evaluate the results. Forty-seven subjects were diagnosed with gingivitis, 70 with chronic periodontitis and 19 with aggressive periodontitis. According to chi-square there was no significant correlation detected between PD (chi2 = 0.73), PI (chi2 = 0.35), BI (chi2 = 0.09) and the presence of the highly leukotoxic A. actinomycetemcomitans. The highly leukotoxic A. actinomycetemcomitans strains were correlated with subjects that were 28 years of age and younger (chi2 = 7.41). There was a significant correlation between highly leukotoxic A. actinomycetemcomitans and aggressive periodontitis (chi2 = 22.06). This study of a Brazilian cohort confirms the strong association between highly leukotoxic A. actinomycetemcomitans strains and the presence of aggressive periodontitis. State University of New York, School of Dental Medicine Universidade Estadual Paulista University of Taubaté Universidade Estadual Paulista

Made available in DSpace on 2019-09-12T16:32:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2013 Introdução: obesidade infantil é importante problema de saúde pública, afetando inclusive criançasmuito precocemente. Objetivo: avaliar o impacto de uma intervenção alimentar e de atividadefísica relativamente simples no estado nutricional de pré-escolares. Método: estudo de intervençãoem creches públicas visando as crianças de 2 a 4 anos incompletos de idade, no Estado de SãoPaulo. A amostra foi de 9 creches abarcando 578 crianças, sendo 264 crianças no grupo intervenção(GI) e 314 no grupo comparação (GC). A intervenção foi voltada para modificações na alimentaçãoe na atividade física, evitando sobrecarregar a rotina das creches, com duração de um ano. Considerou-se um escore z de IMC > 1 (zIMC) e < 2 como risco de sobrepeso, e zIMC > 2 como excesso de peso(sobrepeso e obesidade). A análise foi por cálculos de frequências, comparações de proporções peloχ2, comparações de médias pelo t de Student e cálculo dos coeficientes de correlação de Pearson.Resultados: o GI mostrou correlação inversa entre o zIMC inicial das crianças e a diferença destecom o zIMC ao final da intervenção (rP= -0,39; p 1 (zBMI) and < 2 was considered risk of being overweight, and > 2 zBMI was considered excess weight (overweight and obese). Analysis was done by frequency calculations, comparisons of proportions by Χ2, mean comparisons by t-student and calculations according to Pearson's correlation coefficient. Results: IG showed an inverse correlation between the initial zBMI of the children and the difference with the zBMI at the end of the intervention (rP = -0.39, p