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1. Doctor–couple communication during assisted reproductive technology visits

Author

Arne Luehwink, Daniela Leone, Lidia Borghi, C Filippini, Mauro Costa, L. De Lauretis, Alberto Revelli, F. Tomei, Elena Vegni, G. Tomasi, E. Chelo, S Del Negro, G. Giuffrida, R. Palermo, A. P. Ferraretti, C. Becattini, and Claudia Livi

Subjects
Adult, Male, medicine.medical_specialty, Reproductive Techniques, Assisted, communication content, medicine.medical_treatment, media_common.quotation_subject, MEDLINE, Context (language use), patient-centered score, doctor-patient communication, 03 medical and health sciences, Reproductive Techniques, 0302 clinical medicine, Pregnancy, medicine, Humans, Conversation, 030212 general & internal medicine, media_common, Physician-Patient Relations, 030219 obstetrics & reproductive medicine, Assisted reproductive technology, Socioemotional selectivity theory, Communication, Hawthorne effect, Rehabilitation, Obstetrics and Gynecology, ART, verbal dominance, Female, Italy, Treatment Outcome, Reproductive Medicine, Assisted, Family medicine, Models of communication, Observational study, Psychology
Abstract

Study question What are the characteristics of doctor-couple communication content during actual ART visits? Summary answer Physicians were mainly focused on providing biomedical information, while communication content from couples had a 2-fold focus on providing biomedical information and on positive talk. What is known already Communication aspects in ART seem crucial for clinical decision-making, retention in care and critical conversations with couples due to low treatment success rates. However, no studies have been carried out on the actual interaction between the doctor and the couple in this context. Study design, size, duration This observational study involved 28 clinicians and 160 patients referred to eight Italian ART clinics during a one-year recruitment period. Participants/materials, setting, methods ART visits at eight Italian clinics were videotaped. The visits were coded using the Roter Interaction Analysis System (RIAS), particularly focusing on RIAS composite categories, verbal dominance and patient-centeredness score. Main results and the role of chance A total of 85 visits were eligible for analysis (62% acceptance rate), involving 28 clinicians and 160 patients (including 75 couples). The average visit duration was 37 ± 17.7 min. The mean verbal dominance was 1.9 ± 0.86 (range: 0.72-5.74). Physicians mainly focused on providing biomedical information. Communication content from couples had a 2-fold focus on providing biomedical information and on positive talk. The mean of patient centeredness index (PCI) was 0.51 (SD = 0.28; range 0.08-1.77); visits in which the doctor was a woman or the treatment indication was for heterologous fertilization showed higher PCI scores. Overall, females accounted for 67% of all patient talk. Taking this imbalance into account as expected frequencies for each composite category, males reported significantly more utterances in almost all of the socioemotional categories. Limitations, reasons for caution These results are preliminary and observational and only regard Italy. Communication during visits may have been biased since the professionals who agreed to participate showed an interest in communication issues. Another limitation is a possible Hawthorne effect due to the fact that participants were aware of being videotaped. Wider implications of the findings Our study showed that ART physicians mainly adopted an informative model of communication and a more disease-oriented approach. Findings revealed the complexity of communication content during ART consultations, given its triadic characteristic in which the third party is also a patient; clinicians should be aware of this complex aspect and of the specific male and female perspectives to be taken into account. The results could be useful for training ART professionals. Study funding/competing interest(s) This study was possible thanks to an unconditional grant from Ferring Spa to the Department of Health Sciences, University of Milan. There are no competing interests to declare. Trial registration number N/A.

Published
2018
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2. Patient-centered communication, patient satisfaction, and retention in care in assisted reproductive technology visits

Author

G. Tomasi, A. P. Ferraretti, Alberto Revelli, G. Giuffrida, Daniela Leone, F. Tomei, E. Chelo, Elena Vegni, Claudia Filippini, Lidia Borghi, C. Becattini, R. Palermo, L. De Lauretis, S. Poli, Claudia Livi, Arne Luehwink, and Mauro Costa

Subjects
0301 basic medicine, Adult, Male, medicine.medical_specialty, Adolescent, Reproductive Techniques, Assisted, medicine.medical_treatment, Retention in care, Reproductive medicine, Satisfaction, Context (language use), RIAS, 03 medical and health sciences, 0302 clinical medicine, Patient satisfaction, Patient-centered communication, Patient-Centered Care, Surveys and Questionnaires, Genetics, medicine, Humans, Assisted Reproduction Technologies, Genetics (clinical), Physician-Patient Relations, 030219 obstetrics & reproductive medicine, Assisted reproductive technology, Obstetrics and Gynecology, General Medicine, Middle Aged, Test (assessment), 030104 developmental biology, Reproductive Medicine, Italy, Content analysis, Patient Satisfaction, Family medicine, Conventional PCI, Female, Doctor-patient interaction, Psychology, Developmental Biology
Abstract

PURPOSE: To explore the association between patient-centered communication, patients’ satisfaction, and retention in care in assisted reproductive technology (ART) visits. METHODS: ART visits at eight Italian clinics were videotaped and coded using the Roter Interaction Analysis System, which includes a Patient-Centered Index (PCI), a summary “patient-centered communication” ratio. After the visit, patients completed a satisfaction questionnaire (SATQ). After 3 months, patients were asked about their retention in care. Spearman correlations and Mann-Whitney tests were used to test associations between the study variables; the open-ended item of SATQ was analyzed through content analysis. RESULTS: Eighty-five visits were videotaped (involving 28 gynecologists and 160 patients). PCI score (μ = 0.51 ± 0.28) revealed a more disease-oriented communication during the visit. Patients reported high levels of satisfaction with the visit and identified in the information provision or in the doctor’s humanity or kindness the main reasons of satisfaction. At the follow-up, the majority of the couples declared to have followed the clinicians’ recommendations and to have remained related to the ART center. No associations were found among the study variables, except for a lower male satisfaction among couples who declared to have changed ART clinic. CONCLUSIONS: Contrary to what was expected, the style of physician-patient communication was not found to be associated with patient satisfaction and retention in care. However, patients were highly satisfied and engaged. The actual meaning of a communication that is “patient-centered” in the ART context might be wider, including the couples’ need for information, as suggested by qualitative findings.

Published
2019

3. Posters * Safety & Quality (I.E. Guidelines, Multiple Pregnancy, Outcome, Follow-Up etc.)

Author

P. Ocal, S. Sahmay, T. Irez, H. Senol, I. Cepni, S. Purisa, W. Lin, X. Liu, A. Donjacour, E. Maltepe, P. Rinaudo, M. N. Baumgarten, D. Stoop, P. Haentjes, G. Verheyen, F. De Schrijver, I. Liebaers, M. Camus, M. Bonduelle, P. Devroey, E. C. M. Nelissen, A. P. A. Van Montfoort, E. Coonen, J. G. Derhaag, J. L. H. Evers, J. C. M. Dumoulin, J. R. Costa Lopes, J. Mendes dos Santos, S. Portugal Silva Lima, S. Portugal Silva Souza, T. Rodrigues Pereira, J. P. Barguil Brasileiro, H. Pina, M. L. Lessa, M. Genovese Soares, V. Medina Lopes, C. G. Ribeiro, K. Adami, C. Hughes, G. Emerson, K. Grundy, P. Kelly, E. Mocanu, T. Coelho Cafe, J. B. M. de Souza Costa, N. I. Zavattiero Tierno, S. Singh, S. Vitthala, A. Zosmer, L. Sabatini, A. Tozer, C. Davis, T. Al-Shawaf, Q. V. Neri, D. Monahan, Z. Rosenwaks, G. D. Palermo, E. Kalu, M. Y. Thum, H. A. Abdalla, A. Sazonova, C. Bergh, K. Kallen, A. Thurin-Kjellberg, U. B. Wennerholm, G. Griesinger, K. Doody, H. Witjes, B. Mannaerts, B. Tarlatzis, L. Rombauts, E. Heijnen, M. Marintcheva-Petrova, J. Elbers, A. Koning, M. A. Q. Mutsaerts, A. Hoek, B. W. Mol, R. Fadini, T. Guarnieri, M. Mignini Renzini, R. Comi, M. Mastrolilli, A. Villa, E. Colpi, G. Coticchio, M. Dal Canto, M. Dolleman, S. L. Broer, B. C. Opmeer, B. C. Fauser, F. J. M. Broekmans, P. Alama, A. Requena, J. Crespo, M. Munoz, A. Ballesteros, E. Munoz, M. Fernandez, M. Meseguer, J. A. Garcia-Velasco, A. Pellicer, M. Munk, S. Smidt-Jensen, J. Blaabjerg, C. Christoffersen, S. Lenz, S. Lindenberg, E. Bosch, E. Labarta, F. Cruz, C. Simon, J. Remohi, J. Esler, J. Osborn, C. Boissonnas Chalas, A. Marszalek, P. Fauque, J. P. Wolf, D. De Ziegler, L. Cabanes, P. Jouannet, A. R. Han, C. W. Park, S. W. Cha, H. O. Kim, K. M. Yang, J. Y. Kim, I. O. Song, M. K. Koong, I. S. Kang, R. Roszaman, M. H. Omar, Y. Nazri, Y. W. Azantee, A. Z. Murad, M. R. Zainulrashid, N. Wang, F. Le, L. Y. Wang, G. L. Ding, J. Z. Sheng, H. F. Huang, F. Jin, S. Reinblatt, H. Holzer, W. Y. Son, E. Shalom-Paz, R. C. Chian, W. Buckett, M. Dahan, E. Demirtas, S. L. Tan, A. Revel, Y. Schejter-Dinur, S. Revel-Vilk, R. P. M. G. Hermens, E. van den Boogaard, N. J. Leschot, J. H. A. Vollebergh, R. Bernardus, J. A. M. Kremer, F. van der Veen, M. Goddijn, M. J. Nahuis, N. Kose, N. Bayram, P. G. A. Hompes, B. W. J. Mol, F. van der veen, M. van Wely, J. Van Disseldorp, M. D. Dolleman, K. Broeze, M. De Rycke, L. Petrussa, H. Van de Velde, M. Cerrillo, A. Pacheco, S. Rodriguez, R. Gomez, F. Delagado, J. A. Garcia Velasco, S. Desmyttere, W. Verpoest, C. Staessen, A. De Vos, G. Kohls, F. J. Ruiz, G. De la Fuente, M. Toribio, M. Martinez, V. Soderstrom - Anttila, M. Salevaara, A. M. Suikkari, E. Clua, R. Tur, N. Alcaniz, M. Boada, I. Rodriguez, P. N. Barri, A. Veiga, W. L. D. M. Nelen, I. W. H. Van Empel, B. J. Cohlen, J. S. Laven, J. W. M. Aarts, E. Ricciarelli, J. L. Gomez-Palomares, L. Andres-Criado, E. R. Hernandez, B. Courbiere, M. Aye, J. Perrin, C. Di Giorgio, M. De Meo, A. Botta, J. Castilla Alcala, F. Luceno Maestre, Y. Cabello, J. Hernandez, J. Marqueta, A. Pareja, E. Hernandez, B. Coroleu, L. Helmgaard, B. M. Klein, J. C. Arce, I. W. H. van Empel, J. Boivin, C. M. Verhaak, G. Ding, R. Yin, J. Sheng, H. Huang, F. Mancini, M. J. Gomez, N. M. van den Boogaard, J. W. van der Steeg, P. Hompes, P. Boyer, M. Gervoise-Boyer, L. Meddeb, B. Rossin, F. Audibert, S. Sakian, E. Chan Wong, S. Ma, R. Pathak, M. D. Mustafa, R. S. Ahmed, A. K. Tripathi, K. Guleria, B. D. Banerjee, G. Vela, M. Luna, E. D. Flisser, B. Sandler, M. Brodman, L. Grunfeld, A. B. Copperman, M. Baronio, P. Carrascosa, C. Capunay, J. Vallejos, S. Papier, M. Borghi, C. Sueldo, J. Carrascosa, E. Martin Lopez, A. Marcucci, I. Marcucci, P. Salacone, A. Sebastianelli, L. Caponecchia, N. Pacini, R. Rago, M. Alvarez, O. Carreras, M. Arnoldi, D. Diaferia, M. G. Corbucci, L. De Lauretis, M. J. Kook, J. Y. Jung, J. H. Lee, Y. J. Jung, H. K. Hwang, A. Kang, S. J. An, H. M. Kim, H. C. Kwon, S. J. Lee, M. Satoh, J. Imada, K. Ito, F. Migishima, T. Inoue, Y. Ohnishi, H. Kawato, Y. Nakaoka, A. Fukuda, Y. Morimoto, S. Mourad, R. P. T. M. Grol, N. P. Polyzos, A. Valachis, E. Patavoukas, E. G. Papanikolaou, I. E. Messinis, B. C. Tarlatzis, H. Kang, C. H. Kim, E. Park, S. Kim, H. D. Chae, B. M. Kang, K. S. Jung, H. J. Song, Y. S. Ahn, L. Petkova, I. Canov, T. Milachich, A. Shterev, C. Patrat, K. Pocate, J. C. Juillard, V. Gayet, V. Blanchet, D. de Ziegler, J. W. van der, E. Leushuis, P. Steures, C. Koks, J. Oosterhuis, P. Bourdrez, and P. M. Bossuyt

Subjects
Gynecology, medicine.medical_specialty, business.industry, media_common.quotation_subject, Rehabilitation, Viral screening, Obstetrics and Gynecology, Reproductive Medicine, Oocyte Collection, medicine, Quality (business), Intensive care medicine, business, media_common
Published
2010
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4. Posters * Embryology (Embryo Selection)

Author

K. Versieren, B. Heindryckx, C. Qian, J. Gerris, P. De Sutter, A. Exposito Navarro, A. Ametzazurra, D. Nagore, L. Crisol, F. Aspichueta, R. Mendoza, R. Matorras, M. M. Garcia, J. K. Valley, P. S. Swinton, W. J. Boscardin, T. F. Lue, null P. Rinaudo, M. C. Wu, O. Bern, D. Strassburger, D. Komarovsky, E. Kasterstein, A. Komsky, B. Maslansky, A. Raziel, S. Friedler, Y. Gidoni, R. Ron-El, J. Tang, C. Fang, M. F. Zhang, T. Li, G. L. Zhuang, D. S. Suh, J. K. Joo, J. R. Choi, S. C. Kim, M. S. JO, K. H. Kim, K. S. Lee, M. G. Katz-Jaffe, J. Stevens, S. McCormick, R. Smith, W. B. Schoolcraft, I. Ben-Ami, J. Koch, M. Costello, S. Kilani, A. Namm, A. Arend, M. Aunapuu, Y. M. Choi, J. D. Cho, C. Sipe, E. J. Pelts, J. M. Matthews, S. R. Sanchez, R. L. B. Brohammer, Y. Wagner, J. Liebermann, M. Uhler, A. Beltsos, M. J. Chen, H. F. Guu, Y. F. Chen, Y. J. Yih, J. Y. P. Ho, T. Y. Lin, E. S. C. Ho, F. B. Lopes, R. C. S. Figueira, D. P. A. F. Braga, R. C. Ferreira, T. Aoki, A. Iaconelli, E. Borges, H. Van de Velde, G. Cauffman, A. Verloes, C. De Paepe, J. Sterckx, H. Van Ranst, P. Devroey, H. Tournaye, I. Liebaers, M. A. Santos, G. Teklenburg, N. S. Macklon, D. Van Opstal, G. H. Schuring-Blom, P. J. Krijtenburg, J. de Vreeden-Elbertse, B. C. Fauser, E. B. Baart, S. Cawood, A. Doshi, S. Gotts, P. Serhal, T. Milachich, L. Petkova, D. Barov, A. Shterev, T. C. Esteves, S. T. Balbach, M. J. Arauzo-Bravo, M. J. Pfeiffer, M. Boiani, S. Le Gac, F. van Rossem, T. Esteves, M. Bioani, A. van den Berg, C. Valeri, S. Pappalardo, M. De Felici, C. Manna, H. Ryu, C. Y. Park, S. H. Min, S. K. Choi, C. Park, S. H. Lee, K. R. Kim, H. Jeong, H. J. Chi, C. Wittemer, C. Celebi, S. Viville, F. Luceno Maestre, J. A. Castilla Alcala, J. L. Gomez-Palomares, Y. Cabello, J. Hernandez, J. Marqueta, J. Herrero, E. Vidal, S. Fernandez-Shaw, B. Coroleu, C. McRae, E. Baskind, V. Sharma, J. Fisher, P. Boldi Cotti, C. Colasante, L. Perego, L. De Lauretis, M. Montag, M. Koster, A. Nikolov, H. van der Ven, S. G. Lee, Y. C. Lee, S. M. Kang, Y. J. Kang, Y. K. Shin, J. H. Jung, J. H. Lim, A. Dorfmann, K. Carroll, M. Sisson, M. Geltinger, S. Yap, M. Iwaszko, T. Hara, K. Naruse, K. Matsuura, T. Kodama, K. Sato, Y. Tateaki, J. Tanaka, M. G. Minasi, F. Scarselli, P. Rubino, V. Casciani, A. Colasante, M. Lobascio, E. Alviggi, S. Ferrero, K. Litwicka, E. Iammarrone, F. Cucinelli, P. G. Giannini, A. Tocci, Z. P. Nagy, E. Greco, A. Borini, N. Tarozzi, D. Fiorentin, M. A. Bonu, M. Nadalini, J. Johnson, L. De Santis, V. Bianchi, A. M. Lobascio, L. Arizzi, C. Piscitelli, N. Mesut, H. N. Ciray, A. Mesut, T. Aksoy, M. Bahceci, Y. M. Lee, H. W. Chen, P. Wu, C. R. Tzeng, I. Antonova, M. Yunakova, P. Chaveeva, null A. Shterev, D. Hlinka, M. Dudas, J. Rutarova, J. Rezacova, S. Lazarovska, Y. Aoi, H. Takahashi, H. Saitou, C. Takiue, N. Kawakami, M. Tone, R. Hirata, S. Terada, N. Yoshioka, T. Habara, N. Hayashi, J. Montagut, F. Bonald, N. Guillen, V. Guitard, E. Balu-Genvrin, E. Crae, D. Nogueira, J. Silva, M. Cunha, P. Viana, J. M. Teixeira da Silva, C. Oliveira, A. Goncalves, N. Barros, M. Sousa, A. Barros, C. van de Werken, H. Jahr, J. S. E. Laven, P. Gamiz Izquierdo, J. M. De los Santos, A. Tejera, A. Pellicer, J. L. Romero, A. Galan, C. Albert, M. J. D. l. Santos, T. Adriaenssens, S. Wathlet, I. Segers, G. Verheyen, H. Van De Velde, W. Coucke, J. Smitz, G. Paternot, T. M. D'Hooghe, S. Debrock, C. Spiessens, H. K. Hwang, H. M. Kim, J. H. Lee, Y. J. Jung, A. Kang, M. J. Kook, J. Y. Jung, S. J. An, H. C. Kwon, S. J. Lee, O. Somova, A. Feskov, I. Feskova, N. Chumakova, O. Zozulina, Y. E. Zhilkova, M. Binda, R. Campo, G. Van Kerkhoven, V. Frederickx, A. Serneels, P. Roziers, I. Vranken, A. S. Lopes, A. Van Nuland, S. Gordts, P. Puttemans, M. Valkenburg, A. Rodriguez-Arnedo, J. Ten, J. Guerrero, B. Lledo, M. A. Carracedo, J. A. Ortiz, J. Llacer, R. Bernabeu, K. Usui, Y. Nakajo, M. Ota, H. Hattori, T. Kyoya, T. Takisawa, K. Kyono, A. Ferrieres, M. Poulain, V. Loup, T. Anahory, H. Dechaud, S. Hamamah, J. Eckert, G. Premkumar, F. Lock, S. Brooks, S. Haque, I. T. Cameron, Y. Cheong, T. P. Fleming, N. Prados, M. Ruiz, J. Garcia-Ortega, P. Vime, M. J. Hernaez, M. Crespo, M. Fernandez-Sanchez, S. Hashimoto, N. Kato, K. Saeki, Y. Morimoto, C. O. N. Leung, R. T. K. Pang, W. M. Liu, K. F. Lee, W. S. B. Yeung, T. Wada, T. Elliott, J. Kahn, J. Lowderman, G. Wright, C. Chang, D. Bernal, H. Kort, Z. Nagy, J. M. de los Santos, L. Escrich, N. Grau, M. J. Escriba, M. Escriba, F. Tasker, H. Hamoda, H. Wilner, J. Grace, Y. Khalaf, S. Miyaji, S. Mizuno, L. Horiuchi, A. Haruki, A. Fukuda, T. Utsunomiya, Y. Kumasako, H. Ito, K. Goto, M. Koike, H. Abe, T. Sakamoto, F. Kojima, T. Koshika, L. Muzii, M. C. Magli, L. Gioia, G. Scaravelli, A. P. Ferraretti, L. Gianaroli, A. Capoti, M. Lappi, E. Maggi, L. Scott, A. Finn, B. Kloos, D. Davies, M. Yamada, T. Hamatani, H. Akutsu, N. Chikazawa, S. Ogawa, N. Okumura, Y. Mochimaru, N. Kuji, D. Aoki, Y. Yoshimura, A. Umezawa, V. P. Aprysko, S. A. Yakovenko, E. A. Seregina, E. V. Yutkin, H. Yelke, S. Milik, Z. N. Candan, G. Altin, S. Unal, Z. Atayurt, null Y. Kumtepe, J. T. Chung, W. Y. Son, X. Zhang, S. L. Tan, A. Ao, E. Seli, L. Botros, M. Henson, P. Roos, K. Judge, D. Sakkas, M. S. G. M. S. group, M. Feliciano, D. Monahan, E. Ermolovich, Z. Rosenwaks, G. D. Palermo, E. Mantikou, J. van Echten-Arends, B. Sikkema-Raddatz, F. van der Veen, S. Repping, S. Mastenbroek, M. B. S. Group, V. Wells, M. Y. Thum, H. I. Abdalla, R. Machiya, S. Akimoto, T. Nobuyoshi, N. Yoshii, T. Hosaka, Y. Odawara, F. Vanden Meerschaut, S. Lierman, T. O'Leary, S. Assou, D. Haouzi, F. Pellestor, C. Monzo, J. De Vos, J. Conaghan, E. Fischer, J. Popwell, I. Ryan, P. Chenette, C. Givens, E. Schriock, C. Herbert, Q. V. Neri, M. Camus, P. Haentjens, A. Mugica, M. Esbert, J. M. Molina, N. Garrido, A. Ballesteros, G. Calderon, A. L. S. Rossi, A. M. Rocha, J. R. Alegretti, P. A. Hassun, L. P. Gomes, T. Criscuollo, P. Serafini, E. L. A. Motta, M. Munoz, M. Meseguer, M. Cruz, I. Perez-Cano, B. Gadea, M. Martinez, S. Fortuno, J. Gundersen, E. Selles, J. Betersen, E. Le Meaux, G. Ouandaogo, null S. Hamamah, E. Gismano, I. Cino, F. Calzi, E. Rabellotti, E. Papaleo, S. K. Sunkara, A. Siozos, V. Bolton, P. Braude, T. El-Toukhy, Y. S. Cho, B. Ambruosi, P. Totaro, M. E. Dell'Aquila, G. Gioacchini, D. Bizzaro, E. Giorgini, P. Ferraris, S. Sabbatini, O. Carnevali, P. Knaggs, A. Chau, S. Khalil, G. Trew, S. Lavery, V. P. Jovanovic, R. Gomez, C. M. Sauer, C. J. Shawber, H. H. Outtz, X. Wang, M. V. Sauer, J. Kitajewski, R. C. Zimmermann, E. Mahrous, H. Clarke, I. Virant-Klun, L. Bacer-Kermavner, J. Mivsek, T. Tomazevic, B. Pozlep, B. Zorn, E. Vrtacnik-Bokal, I. Dundure, J. Bazarova, V. Fodina, J. Brikune, J. Lakutins, B. Jee, J. Jo, J. Lee, C. Suh, S. Kim, S. Moon, Y. Shufaro, M. Lebovich, E. Aizenman, A. Simon, N. Laufer, null A. Saada Reisch, M. A. Ribeiro, A. Pinto, F. Gomes, J. L. Silva Carvalho, H. Almeida, F. C. Massaro, C. G. Petersen, A. L. Mauri, L. F. I. Silva, A. P. M. Nicoletti, M. Cavagna, A. Pontes, R. L. R. Baruffi, J. B. A. Oliveira, J. G. Franco, A. Valcarcel, M. I. Viglierchio, M. Tiveron, M. Guidobono, R. Inza, M. Vilela, A. Kenny, C. Lombardi, and G. Marconi

Subjects
biology, business.industry, Rehabilitation, Embryogenesis, Seven in absentia homolog, Obstetrics and Gynecology, SIAH1, Cell biology, Ubiquitin ligase, Reproductive Medicine, Immunology, microRNA, biology.protein, Medicine, business
Published
2010
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5. Morphological Selection of Human Spermatozoa in Cervical Mucus 'In Vivo'

Author

D. Olivares, Guido Ragni, L. De Lauretis, O Bestetti, Guercilena S, and R Di Pietro

Subjects
Male, urogenital system, Urology, Coitus, Motility, General Medicine, Anatomy, Biology, Postcoital test, Spermatozoa, Mucus, Cervical mucus, Normal spermatozoa, Endocrinology, medicine.anatomical_structure, In vivo, Cervix Mucus, Sperm Motility, medicine, Humans, Female, Cervical canal, Sperm motility
Abstract

Spermatozoa morphology at two levels in the cervical canal was compared, at the external and internal uterine orifices, in samples taken "in vivo" during 21 postcoital tests (P.C.T.s). P.C.T.s with cervical mucus, with Moghissi scores lower than 10 and exo- and endocervical pH less than 7 were excluded. At the upper level of the cervical canal, 12 of the 21 P.C.T. showed more than 5% more normal spermatozoa than at the lower level of the canal (in 5 of these P.C.T. the increase was greater than 10%). Selection for normal heads was seen in 2 and for normal tails in 4. The P.C.T. that selected for normal tails all had greater than 10% abnormal tails at the external orifice. These results confirm that there is some selection of spermatozoa during passage through the cervical canal. Above all, the selection appears to exclude those spermatozoa with defective locomotive mechanisms (abnormal tails), which indicates that the mucus acts as a "passive filter" with selection depending on the spermatozoa themselves in relation to motility.

Published
2009
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6. Semen preparation by standard swim-up versus swim-up with test yolk buffer incubation in intrauterine insemination: a randomized study

Author

P. G. Crosignani, L. Perotti, Fabio Parazzini, A. Dalla Serra, Walter Vegetti, Guido Ragni, Paola Maggioni, G D'Ambrogio, S Pellegrini, and L. De Lauretis

Subjects
Male, Infertility, medicine.medical_specialty, medicine.medical_treatment, Semen, Biology, Andrology, Ovulation Induction, Pregnancy, medicine, Humans, Prospective Studies, Tromethamine, Incubation, Infertility, Male, Insemination, Artificial, Homologous, Unexplained infertility, Gynecology, Artificial insemination, Rehabilitation, Obstetrics and Gynecology, medicine.disease, Pregnancy rate, Glucose, Reproductive Medicine, Sperm Motility, Female, Ovulation induction, human activities
Abstract

In order to compare the standard swim-up semen preparation with and without test yolk buffer (TYB) incubation in intrauterine insemination (IUI), we conducted a prospective multicentre randomized trial. A total of 121 infertile couples with male factor (n = 52) or unexplained infertility (n = 69) was randomly assigned to two groups following ovulation induction. Semen was prepared by standard swim-up in group A (n = 64) and by swim-up followed by TYB incubation in group B (n = 57). A maximum of two IUI cycles was performed. A total of 104 cycles was performed in the swim-up group and 90 in the TYB group. Overall, 15 pregnancies were achieved in group A and 23 in group B, with an overall pregnancy rate of 24.8 and 50.0% per patient respectively (chi2(1), P < 0.05). In the male factor group, pregnancy was achieved in six out of 24 couples (25%) following standard swim-up and in six out of 28 (21.4%) following swim-up and TYB incubation (chi2(1), not significant). In the unexplained infertility group, pregnancy was recorded in nine out of 40 couples (22.5%) following standard swim-up and in 17 out of 29 couples (58.6%) following swim-up and TYB incubation (chi2(1), P < 0.05).

Published
1998
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7. Semen Parameters and Conception Rates after Intraperitoneal Insemination

Author

Walter Vegetti, Liliane Chatenoud, P. G. Crosignani, L. Perotti, Fabio Parazzini, Guido Ragni, L. De Lauretis, and F. Sapienza

Subjects
Male, medicine.medical_specialty, Pregnancy Rate, medicine.medical_treatment, Semen, Male infertility, Pregnancy, medicine, Humans, Peritoneal Cavity, Insemination, Artificial, Unexplained infertility, Gynecology, Sperm Count, Obstetrics, business.industry, Artificial insemination, Pregnancy Outcome, Obstetrics and Gynecology, Prognosis, medicine.disease, Reproductive Medicine, Sperm Motility, Gestation, Female, Intraperitoneal insemination, business, Cohort study
Abstract

Objective: To analyze the reproductive outcome in infertile couples which underwent intraperitoneal insemination (IPI). Methods: We analyzed a series of 216 couples who underwent IPI. Indications for treatment were unexplained infertility in 51 couples and male factor in 165. The 51 couples with unexplained infertility underwent a total of 71 cycles (20 couples underwent a second IPI cycle). The 165 couples with male factor underwent 243 cycles (165 first cycles and 78 second cycles). Results: Out of the 314 cycles performed, a total of 41 clinical pregnancies were observed, with a corresponding conception rate of 13.1%. The values of conception rates for unexplained and male factor infertility were 21.1 (based on 15 pregnancies) and 10.7% (based on 26 pregnancies), respectively. Out of the 41 pregnant women, 26 gave birth to a child, thus the overall livebirth rate was 8.3% (12.7 and 7.0%, respectively, for unexplained infertility and male factor diagnostic subgroups). Considering the couples with unexplained infertility, the conception rates were 9.0, 30.8 and 20.0% for strata of 10 millions of inseminated spermatozoa. The corresponding values were 6.8, 12.5 and 18.5%, respectively, in couples with male factor infertility (χ21 trend p Conclusion: In conclusion, this series provides quantitative estimates of pregnancy rates after IPI in Italian couples with unexplained infertility or male infertility and suggested that the number of motile sperm inseminated is a determinant of pregnancy with this technique.

Published
1997
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9. Pregnancy after direct intraperitoneal insemination of spermatozoa from a man with severe infertility, after unsuccessful application of other methods of assisted fertilization

Author

P. G. Crosignani, Guido Ragni, G.C. Lombroso, L. De Lauretis, M.D. Olivares, and C. Cristiani

Subjects
Adult, Male, Infertility, medicine.medical_specialty, medicine.medical_treatment, media_common.quotation_subject, Fertility, Insemination, Male infertility, Human fertilization, Pregnancy, medicine, Humans, Infertility, Male, Insemination, Artificial, media_common, Unexplained infertility, Gynecology, Assisted reproductive technology, Obstetrics, business.industry, Rehabilitation, Obstetrics and Gynecology, medicine.disease, Reproductive Medicine, Female, Pregnancy, Multiple, business, Injections, Intraperitoneal
Abstract

Direct intraperitoneal insemination (DIPI) is one of the least invasive strategies of assisted reproduction. Unexplained infertility and male sub-fertility are the major indications for DIPI. It is otherwise well known that assisted procreation gives poor results in severe male infertility. This is a report of a pregnancy that occurred as a result of a direct intraperitoneal insemination of prepared spermatozoa characterized by a particularly severe astheno-teratozoospermia in a couple unsuccessfully treated with other, more invasive methods of assisted fertilization.

Published
1990
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10. P-092. Ovarian morphometric analysis and ovulation induction outcome

Author

S. Piloni, P. G. Crosignani, S. Saraifogher, L. Perotti, A. Ranzi, L. De Lauretis, Guido Ragni, and E. Guermandi

Subjects
Andrology, Reproductive Medicine, Morphometric analysis, business.industry, medicine.medical_treatment, Rehabilitation, Obstetrics and Gynecology, Medicine, Ovulation induction, business, Outcome (game theory)
Published
1997
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12. P-174. Pattern of inheritance in familial premature ovarian failure

Author

Walter Vegetti, G. Testa, Maria Grazia Tibiletti, Pier Francesco Bolis, P. G. Crosignani, Leda Dalprà, F. Alagna, M. Lalia, and L. De Lauretis

Subjects
Inheritance (object-oriented programming), Reproductive Medicine, Rehabilitation, medicine, Obstetrics and Gynecology, Biology, medicine.disease, Bioinformatics, Premature ovarian failure
Published
1997
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13. Intraperitoneal insemination in the treatment of male and unexplained infertility

Author

L. De Lauretis, L. Perotti, Guido Ragni, Olivares, P. G. Crosignani, and Gcl Finzi

Subjects
Gynecology, Pregnancy, medicine.medical_specialty, Assisted reproductive technology, In vitro fertilisation, Obstetrics, business.industry, medicine.medical_treatment, Artificial insemination, Obstetrics and Gynecology, General Medicine, medicine.disease, Insemination, Male infertility, medicine, Gamete intrafallopian transfer, business, Unexplained infertility
Abstract

For couples with unexplained or male infertility, intraperitoneal (IP) insemination in induced cycles is a method that increases the chance of fertilization. Seventy-seven couples with male subfertility were subjected to 120 IP insemination cycles and 31 with unexplained sterility to 44 cycles. As a consequence of the treatment, 23 pregnancies were obtained, with pregnancy rates of 23% per cycle and 32% per patient for unexplained infertility and 11% and 17% for male subfertility. Pregnancy loss rate was quite elevated: 9 clinical abortions and 1 ectopic. Intraperitoneal insemination appears to be a relatively noninvasive way to deal with unexplained or male factor infertility, well worth trying before moving on to more invasive and costly approaches, such as gamete intrafallopian transfer or in vitro fertilization.

Published
1992
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14. IVF: Induction of ovulation in poor responders

Author

M.D. Olivares, V. Cavioni, Guido Ragni, A. Caccamo, P. G. Crosignani, C. Scarduelli, C. Cristiani, G.C. Lombroso, H. Wyssling, L. De Lauretis, and Leda Dalprà

Subjects
Adult, Agonist, medicine.medical_specialty, medicine.drug_class, media_common.quotation_subject, Poor responder, Fertilization in Vitro, Buserelin, Chorionic Gonadotropin, Biochemistry, Andrology, Endocrinology, Ovulation Induction, Internal medicine, Humans, Medicine, Ovulation, Menstrual Cycle, reproductive and urinary physiology, media_common, Follicle growth, biology, business.industry, female genital diseases and pregnancy complications, HMG-CoA reductase, Oocytes, biology.protein, Female, Norethindrone, business, hormones, hormone substitutes, and hormone antagonists, medicine.drug
Abstract

All IVF programs have a consistent rate of failure in inducing ovulations. Pharmacological induction of ovulation is otherwise crucial for an IVF program because of the need for more than one ovum. Since it is well known that the best candidates for HMG treatment are hypogonadotropic women a short reversible hypogonadotropic state was induced in IVF patients by LH-RH agonist (Buserelin). Superovulation was then achieved with very high initial doses of FSH (Metrodin) in order to maximize the ovarian response. This technique used in 116 IVF women induced a satisfactory follicle growth even in 70% of the patients already poorly responsive to HMG stimulation.

Published
1989
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15. Ovarian stimulation of IVF patients: effects of the reversible hypogonadotrophic state induced by GnRH agonist

Author

M.D. Olivares, C. Cristiani, L. De Lauretis, L. Perotti, Guido Ragni, A. Caccamo, P. G. Crosignani, C. Scarduelli, Leda Dalprà, H. Wyssling, G.C. Lombroso, and V. Cavioni

Subjects
Adult, Agonist, endocrine system, medicine.medical_specialty, Menotropins, medicine.drug_class, medicine.medical_treatment, media_common.quotation_subject, Fertilization in Vitro, Luteal phase, Buserelin, Clomiphene, Ovulation Induction, Internal medicine, medicine, Humans, Ovarian follicle, Ovulation, Menstrual Cycle, Menstrual cycle, media_common, business.industry, Rehabilitation, Obstetrics and Gynecology, Endocrinology, medicine.anatomical_structure, Reproductive Medicine, Drug Therapy, Combination, Female, Ovulation induction, Norethindrone, business, medicine.drug
Abstract

Multiple ovulation was induced in 122 hypogonadotrophic IVF patients with large doses of HMG. The hypogonadotrophic state, short and reversible, was obtained by nasal administration of a GnRH agonist (200 micrograms, five times per day). In the 97 induced cycles, a mean of 9.1 follicles was recorded. A comparison of the results obtained for 36 patients who had already been treated with clomiphene and HMG showed both significantly more follicles per cycle (8.5 versus 3.0) and an increase in oocytes retrieved (6.7 versus 1.3) when treated with the agonist and HMG. In addition 11 of 18 already poorly responsive patients had normal responses. The luteal phase was supported by either HCG or progesterone injection. Plasma progesterone profiles were satisfactory and, as expected, the highest progesterone concentrations were associated with HCG treatment.

Published
1988
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16. Gonadal function in male heroin and methadone addicts

Author

D Sghedoni, V. Gambaro, Guido Ragni, O Bestetti, and L. De Lauretis

Subjects
Adult, Male, endocrine system, medicine.medical_specialty, Adolescent, medicine.drug_class, Urology, Endocrinology, Diabetes and Metabolism, Heroin, Follicle-stimulating hormone, Semen, Internal medicine, mental disorders, Testis, medicine, Humans, Testosterone, Sperm Count, business.industry, Heroin Dependence, medicine.disease, Androgen, Opioid-Related Disorders, Prolactin, Endocrinology, Reproductive Medicine, Oligospermia, Gonadotropins, Pituitary, Sperm Motility, business, Luteinizing hormone, Methadone, medicine.drug
Abstract

Gonadal function was elevated in 80 male heroin and/or methadone addicts by measuring basal plasma levels of follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin (PRL) and testosterone. In 41 subjects semen analyses were also undertaken. Three groups were distinguished consisting of 15 heroin addicts, 42 undergoing methadone treatment but continuing to take heroin, and 23 taking only methadone. All patients had normal plasma levels of FSH, LH and testosterone. Prolactin levels were normal in all subjects except for the 15 heroin addicts, in whom they were significantly higher than in controls (P less than 0.025). Semen analyses from all of the heroin addicts and from the dual heroin-methadone users were abnormal, whereas only 10 out of 22 (45%) of the methadone takers were pathological. In all cases asthenospermia was one of the abnormalities (100%). Twenty-four per cent also showed teratospermia and hypospermia and 17% showed oligozoospermia. Such seminal pathology, especially of forward motility, even in combination with normal hormone levels, might be an early indication of heroin toxicity to the male reproductive tract.

Published
1988

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