1. Physical Activity Attenuates the Obesity-Induced Dysregulated Expression of Brown Adipokines in Murine Interscapular Brown Adipose Tissue
- Author
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Takako Kizaki, Sachiko Yamamoto, Takuya Sakurai, Eriko Nozaki, and Toshiyuki Fukutomi
- Subjects
medicine.medical_specialty ,obesity ,QH301-705.5 ,Galectin 3 ,Adipokine ,physical activity ,brown adipocyte differentiation ,Biology ,Catalysis ,Article ,Inorganic Chemistry ,Mice ,Adipokines ,Adipose Tissue, Brown ,Internal medicine ,Physical Conditioning, Animal ,Brown adipose tissue ,Coactivator ,medicine ,Animals ,Physical and Theoretical Chemistry ,Biology (General) ,Receptor ,Molecular Biology ,QD1-999 ,Spectroscopy ,Binding protein ,Organic Chemistry ,Cell Differentiation ,brown adipose tissue ,General Medicine ,Peroxisome ,medicine.disease ,Obesity ,Computer Science Applications ,brown adipokine ,Chemistry ,medicine.anatomical_structure ,Endocrinology ,Adipocytes, Brown ,Gene Expression Regulation ,Lipogenesis - Abstract
In recent years, brown adipose tissue (BAT), which has a high heat-producing capacity, has been confirmed to exist even in adults, and it has become a focal point for the prevention and the improvement of obesity and lifestyle-related diseases. However, the influences of obesity and physical activity (PA) on the fluid factors secreted from BAT (brown adipokines) are not well understood. In this study, therefore, we focused on brown adipokines and investigated the effects of obesity and PA. The abnormal expressions of gene fluid factors such as galectin-3 (Lgals3) and Lgals3 binding protein (Lgals3bp), whose proteins are secreted from HB2 brown adipocytes, were observed in the interscapular BAT of obese mice fed a high-fat diet for 4 months. PA attenuated the abnormalities in the expressions of these genes. Furthermore, although the gene expressions of factors related to brown adipocyte differentiation such as peroxisome proliferator-activated receptor gamma coactivator 1-α were also down-regulated in the BAT of the obese mice, PA suppressed the down-regulation of these factors. On the other hand, lipogenesis was increased more in HB2 cells overexpressing Lgals3 compared with that in control cells, and the overexpression of Lgals3bp decreased the mitochondrial mass. These results indicate that PA attenuates the obesity-induced dysregulated expression of brown adipokines and suggests that Lgals3 and Lgals3bp are involved in brown adipocyte differentiation.
- Published
- 2021