Author: "Bao, Qi" / Topic: medicine - Searchworks@Jio Institute Digital Library Search Results

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42 results on '"Bao, Qi"'

1. Didymium arenosum, a myxomycete new to science from the confluence of deserts in northwestern China

Author: Shuwei Wei, Shu Li, Pu Liu, Bao Qi, Qi Wang, and Yu Li
Subjects: Didymium, Morphology, Phylogeny, Scanning Electron Microscope, SSU rDNA, The life cycle, Medicine, Biology (General), QH301-705.5
Abstract: A new myxomycete species, Didymium arenosum, was described based on morphological evidence and phylogenetic analyses. The species was discovered in the arid region at the confluence of the Badain Jaran desert and Tengger desert on the leaves of Betula platyphylla and was cultivated in a moist chamber culture. Morphologically, the species is distinguished by the greenish-yellow calcium carbonate crystals on the surface and the spores covered with small warts, some of which are connected into a short line. A phylogenetic analysis of D. arenosum strongly supports its classification as a separate clade. The spore to spore agar culture of D. arenosum requires 23 days, and this study provides a detailed description of its life cycle.
Published: 2024
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2. Distribution characteristics and diversity of myxomycetes in three parallel rivers in Yunnan, China

Author: Xiangyang Zhu, Odeshnee Naicker, Zhanwu Peng, Bao Qi, Qi Wang, and Yu Li
Subjects: Medicine, Science
Published: 2024

3. Effectiveness of price limits: Evidence from China's ChiNext market.

Author: Bao Qi
Subjects: Medicine, Science
Abstract: Starting from August 24, 2020, the daily stock price limits in China's ChiNext market have been adjusted from 10% to 20%. We use this reform to study the effectiveness of price limits in China's stock market. We test four hypotheses about price limits: delayed price discovery, volatility spillover, trading interference, and magnet effect. Using the event study method, we examine the differences in the behavior of stock price, trading volume, and volatility before and after the reform. We confirm the delayed price discovery, volatility spillover and trading interference hypothesis of price limits, and find that these negative effects of price limits are more serious when lower limits are hit. In addition, we examine the distribution of large price movements before and after the reform and find no evidence of the magnet effect of price limits. The present research has important implications for policymakers and investors in China's stock market.
Published: 2023
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4. The fungal community in non-rhizosphere soil of Panax ginseng are driven by different cultivation modes and increased cultivation periods

Author: Yu Bao, Bao Qi, Wei Huang, Bao Liu, and Yu Li
Subjects: Ginseng, Non-rhizosphere soil, Amplicon sequencing, Fungi, Land fallow, Artificial cultivation, Medicine, Biology (General), QH301-705.5
Abstract: Continuous cropping obstacles severely hindered the sustained development of the ginseng industry. Among the obstacles, an imbalance of soil microbiome community was considered one of the major culprits. The fungal community is an essential part of the soil microbiome community. Extensive characterization of the fungal community composition and variation during ginseng cultivation will help us understand the mechanism underlying continuous cropping obstacles. By using a high-throughput amplicon sequencing method, the non-rhizospheric fungal community of farmland cultivated ginseng of 2 years old (C2) and 5 years old (C5), understory wild ginseng of 15 years old (W15) and 35 years old (W35), fallow fields which have been abandoned for 10 (F10) years were characterized. Farmland cultivated ginseng and understory wild ginseng harbored distinct non-rhizospheric fungal communities, and extension of cultivation periods enlarged the fungal community difference between two cultivation modes. Extended cultivation periods significantly decreased the OTU richness and PD whole tree indices, and OTU number and cultivation periods were negatively correlated. Extension of cultivation periods led to an increased abundance of pathotrophs. Still, the increased abundance of pathotrophs may not be the leading cause of severe continuous cropping obstacles in farmland cultivated ginseng. Compared with understory wild ginseng, farmland cultivated ginseng had a lower abundance of symbiotrophs and a higher abundance of saprotrophs. This changed symbiotrophs/saprotrophs ratio may have some correlation with the severe continuous cropping obstacles that occurred in farmland cultivated ginseng. Fallowing on the fungal community of the non-rhizosphere soil was generally opposite of that of extension of ginseng cultivation periods. The impacts of farmland cultivation on the fungal community of the non-rhizosphere soil can last for decades, even if the following is practiced.
Published: 2020
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5. Novel Reassortant Avian Influenza A(H5N6) Viruses in Humans, Guangdong, China, 2015

Author: Yong-Yi Shen, Chang-Wen Ke, Qian Li, Run-Yu Yuan, Dan Xiang, Wei-Xin Jia, Yun-Di Yu, Lu Liu, Can Huang, Wen-Bao Qi, Reina Sikkema, Jie Wu, Marion Koopmans, and Ming Liao
Subjects: H5N6, avian influenza, A(H5N6), novel, reassortant, viruses, Medicine, Infectious and parasitic diseases, RC109-216
Published: 2016
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6. Recurrent Hemoptysis After Bronchial Artery Embolization: Prediction Using a Nomogram and Artificial Neural Network Model

Author: Li-Jun Guan, Sheng Xu, Xue-Jun Zhang, Bao-Qi Shi, and Yong-Sheng Tan
Subjects: Male, Hemoptysis, medicine.medical_specialty, medicine.medical_treatment, Artificial neural network model, Bronchial Arteries, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Recurrence, Internal medicine, medicine.artery, Humans, Medicine, Radiology, Nuclear Medicine and imaging, Embolization, Lung cancer, Aged, Retrospective Studies, Receiver operating characteristic, business.industry, Hazard ratio, General Medicine, Middle Aged, Nomogram, medicine.disease, Embolization, Therapeutic, Training cohort, Nomograms, 030220 oncology & carcinogenesis, Female, Neural Networks, Computer, business, Bronchial artery
Abstract: OBJECTIVE. The purpose of this study was to develop an effective nomogram and artificial neural network (ANN) model for predicting recurrent hemoptysis after bronchial artery embolization (BAE). MATERIALS AND METHODS. The institutional ethics review boards of the two participating hospitals approved this study. Patients with hemoptysis who were treated with BAE were allocated to either the training cohort (Hospital A) or the validation cohort (Hospital B). The predictors of recurrent hemoptysis were identified by univariable and multivariable analyses in the training cohort. A nomogram and ANN model were then developed, and the accuracy was validated by the Harrell C statistic and ROC curves in both the training and validation cohorts. RESULTS. A total of 242 patients (training cohort, 141; validation cohort, 101) were enrolled in this study. The univariable and multivariable analyses revealed that age of 60 years old or older (hazard ratio [HR], 3.921; 95% CI, 1.267-12.127; p = 0.018), lung cancer (HR, 18.057; 95% CI, 4.124-79.068; p 0.16) according to the nomogram.
Published: 2020

7. Prognostic nomogram for the combination therapy of percutaneous catheter drainage and antibiotics in pyogenic liver abscess patients

Author: Bao-Qi Shi, Xue-Jun Zhang, Sheng Xu, Lu-Meng Chao, and Yong-Sheng Tan
Subjects: Male, medicine.medical_specialty, Percutaneous, Combination therapy, Urology, Gastroenterology, 030218 nuclear medicine & medical imaging, 03 medical and health sciences, 0302 clinical medicine, Internal medicine, medicine, Humans, Radiology, Nuclear Medicine and imaging, Retrospective Studies, Pyogenic liver abscess, Radiological and Ultrasound Technology, Septic shock, business.industry, Retrospective cohort study, Middle Aged, Hepatology, Nomogram, Prognosis, medicine.disease, Combined Modality Therapy, Anti-Bacterial Agents, Nomograms, Liver Abscess, Pyogenic, 030220 oncology & carcinogenesis, Drainage, Female, Tomography, X-Ray Computed, business, Liver abscess
Abstract: To identify the predictors for recovery of pyogenic liver abscess (PLA) patients treated with percutaneous catheter drainage (PCD) and antibiotics, and then develop an effective nomogram to predict the recovery time. The retrospective study included consecutive PLA patients treated with PCD and antibiotics. We defined the overall recovery time (ORT) as the time from the PCD procedure to the time of clinical success or failure. Based on the ORT, its predictors were identified with univariate and multivariate analyses. Then, a nomogram was developed to predict the ORT, and was internally validated by using Harrell’s c statistic. A total of 116 patients and 142 PCD procedures with a median ORT of 15.0±10.6 days were included. Gas-formation (GF; HR: 0.486 [95% CI 0.312–0.757]; P = 0.001), diabetes mellitus (DM; HR: 0.455 [95% CI 0.303–0.682]; P
Published: 2019

8. Anxiolytic and anti-depressant effects of hydroalcoholic extract from Erythrina variegata and its possible mechanism of action

Author: Hong-Biao Chu, Bao-Qi Rao, Yun-Jing Li, Yue-De Tan, Bin-Bin Cheng, and Ling-Shan Zhou
Subjects: Forced swimming, Elevated plus maze, biology, business.industry, medicine.drug_class, General Medicine, Pharmacology, biology.organism_classification, Anxiolytic, Open field, chemistry.chemical_compound, Mechanism of action, chemistry, Medicine, Anti depressant, anti-depressant, Erythrina variegata, elevated plus maze, forced swimming, neurotransmitters, medicine.symptom, business, Neurotransmitter, Erythrina
Abstract: Background: Erythrina variegata has been widely used as a traditional medicine. Objective: The study was designed to evaluate the anxiolytic and anti-depressant effects of an extract from Erythrina variegata. Methods: The extract was evaluated for anxiolytic and anti-depressant action using the elevated plus maze, light/dark box, open field, forced swimming and tail suspension tests in mice. The mechanism of action was further elucidated using high-performance liquid chromatography with fluorescence detection methods to assay the levels of five neurotransmitters in brain. Results: The extract exhibited significant increase in the percentage of the open arms entries and the time spent in the open arms in the elevated plus maze test. The results of the light/dark box test revealed a significant increase in the amount of time spent in the light chamber. Extract- treated mice also produced significant increase in the number of crossings and rearings in the open field test.In the forced swimming and tail suspension tests, the extract was able to promote significant decrease in the immobility time. In addition, the extract significantly altered the levels of five neurotransmitters in the brain tissue. Conclusion: These findings suggest that Erythrina variegata presents potential anxiolytic and anti-depressant activity, and the mechanism may be related to the alteration of neurotransmitter levels. Keywords: Anxiolytic; anti-depressant; Erythrina variegata; elevated plus maze; forced swimming; neurotransmitters.
Published: 2019

9. Transfer of mitochondria from mesenchymal stem cells derived from induced pluripotent stem cells attenuates hypoxia-ischemia-induced mitochondrial dysfunction in PC12 cells

Author: Yuelin Zhang, Yan Yang, Haiwei He, Gen Ye, Yimei Hong, Bao-Qi Yu, Xin Li, and Wei You
Subjects: 0301 basic medicine, induced pluripotent stem cells, Mitochondrion, lcsh:RC346-429, Flow cytometry, tunneling nanotubes, 03 medical and health sciences, 0302 clinical medicine, mitochondrial membrane potential, Developmental Neuroscience, medicine, Induced pluripotent stem cell, Cell damage, lcsh:Neurology. Diseases of the nervous system, mesenchymal stem cells, medicine.diagnostic_test, Chemistry, Mesenchymal stem cell, apoptosis, mitochondrial transfer, PC12 cells, medicine.disease, brain injury, hypoxia-ischemia, Chromatin, Cell biology, 030104 developmental biology, Apoptosis, Stem cell, 030217 neurology & neurosurgery, Research Article
Abstract: Mitochondrial dysfunction in neurons has been implicated in hypoxia-ischemia-induced brain injury. Although mesenchymal stem cell therapy has emerged as a novel treatment for this pathology, the mechanisms are not fully understood. To address this issue, we first co-cultured 1.5 × 105 PC12 cells with mesenchymal stem cells that were derived from induced pluripotent stem cells at a ratio of 1:1, and then intervened with cobalt chloride (CoCl2) for 24 hours. Reactive oxygen species in PC12 cells was measured by Mito-sox. Mitochondrial membrane potential (?Ψm) in PC12 cells was determined by JC-1 staining. Apoptosis of PC12 cells was detected by terminal deoxynucleotidal transferase-mediated dUTP nick end-labeling staining. Mitochondrial morphology in PC12 cells was examined by transmission electron microscopy. Transfer of mitochondria from the mesenchymal stem cells derived from induced pluripotent stem cells to damaged PC12 cells was measured by flow cytometry. Mesenchymal stem cells were induced from pluripotent stem cells by lentivirus infection containing green fluorescent protein in mitochondria. Then they were co-cultured with PC12 cells in Transwell chambers and treated with CoCl2 for 24 hours to detect adenosine triphosphate level in PC12 cells. CoCl2-induced PC12 cell damage was dose-dependent. Co-culture with mesenchymal stem cells significantly reduced apoptosis and restored ?Ψm in the injured PC12 cells under CoCl2 challenge. Co-culture with mesenchymal stem cells ameliorated mitochondrial swelling, the disappearance of cristae, and chromatin margination in the injured PC12 cells. After direct co-culture, mitochondrial transfer from the mesenchymal stem cells stem cells to PC12 cells was detected via formed tunneling nanotubes between these two types of cells. The transfer efficiency was greatly enhanced in the presence of CoCl2. More importantly, inhibition of tunneling nanotubes partially abrogated the beneficial effects of mesenchymal stem cells on CoCl2-induced PC12 cell injury. Mesenchymal stem cells reduced CoCl2-induced PC12 cell injury and these effects were in part due to efficacious mitochondrial transfer.
Published: 2019

10. Chinese Expert Proposal on the Diagnosis and Management of Pemphigus Vulgaris (2020)#

Author: Dermatology Branch of China, International Exchange and Promotion Association for Medical and Healthcare, Ya-Gang Zuo, Li Li, Jin-Bo Chen, Liu-Qing Chen, Su-Ying Feng, Wei Li, Xiao-Qun Luo, Meng Pan, Gang Wang, Ting Xiao, Bao-Qi Yang, Kang Zeng, Gui-Ying Zhang, and Hong-Zhong Jin
Subjects: medicine.medical_specialty, Infectious Diseases, business.industry, Pemphigus vulgaris, lcsh:Dermatology, medicine, Dermatology, lcsh:RL1-803, medicine.disease, business
Abstract: Pemphigus vulgaris (PV) is a life-threatening autoimmune bullous disease that causes blisters and erosions on the skin and mucous membranes to standardize the diagnosis and treatment of PV, Chinese experts in this field were invited to make recommendations which are presented in this article. Pemphigus vulgaris can be divided into cutaneous mucous type, cutaneous type and mucous mucous dominant type according to the different clinical manifestations. The pathological manifestations of PV are acantholysis above the basal layer. The diagnosis of PV and the assessment of the severity of the disease are made in accordance with the clinical manifestations, histopathological features, immunofluorescence assay results, and detection of pathogenic serum antibodies. The first-line treatment of PV comprises systemic glucocorticoids. Early combination with immunosuppressive agents or rituximab is recommended for moderate and severe PV. Intravenous immunoglobulin administration is recommended for patients being treated with rituximab. Plasma exchange and stem cell transplantation can be performed if necessary. During the course of therapy, the disease activity should be closely monitored, and actions should be taken to prevent adverse reactions.
Published: 2019

11. Efficacy and safety of Run Zao Zhi Yang capsule on chronic eczema: a multiple-center, randomized, double-blind, placebo-controlled clinical study

Author: Furen Zhang, Jie Zheng, Sen Yang, Qing Guo, Kun Chen, Linfeng Li, Guo-Zhen Tan, Hui Li, Heng Gu, Bao-Qi Yang, Jinhua Xu, Qianjin Lu, and Dan Huang
Subjects: Adult, Male, medicine.medical_specialty, Randomization, Eczema, Dermatology, Traditional Chinese medicine, Placebo, Severity of Illness Index, law.invention, Double blind, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, Double-Blind Method, Randomized controlled trial, law, Internal medicine, medicine, Humans, Medicine, Chinese Traditional, Aged, 030203 arthritis & rheumatology, Chronic eczema, business.industry, Capsule, Middle Aged, humanities, Clinical trial, Chronic Disease, Female, business, human activities
Abstract: Background: Run Zao Zhi Yang capsule (RZZYC) has been widely applied for eczema treatment as a traditional Chinese medicine, while its efficacy has not been scientifically investigated.Objective: W...
Published: 2019

12. 1,25-Dihydroxyvitamin D3 affects gastric cancer progression by repressing BMP3 promoter methylation

Author: Xiao-Juan Shi, Ping Song, Liang-Liang Cai, Bao-Qi Huang, Hui-Ming Ye, Ye Zhao, Hui-Ling Wang, and Chi-Meng Tzeng
Subjects: 0301 basic medicine, medicine.diagnostic_test, Cancer, Biology, medicine.disease, Bisulfite, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Oncology, Western blot, CpG site, 030220 oncology & carcinogenesis, DNA methylation, Cancer cell, Cancer research, medicine, Adenocarcinoma, Pharmacology (medical), Electrophoretic mobility shift assay
Abstract: Background Vitamin D3 has been known to have an anticancer effect, but the mechanisms underlying this is poorly explored. The present study aimed to investigate the antitumor role of vitamin D3 on gastric cancer and mechanisms. Methods The Roche Elecsys platform was applied in retrospective studies to detect the role of 25-hydroxylvitamin D3 in adenocarcinoma and colony formation assay was conducted to verify the effect of 1, 25-dihydroxyvitamin D3 on the proliferation of gastric cancer cells. After the identification of hypermethylation of BMP3 CpG islands by bisulfite genomic sequencing (BGS), we further investigated the relationship of BMP3 expression and gastric carcinogenesis by Western blot analysis and gel electrophoresis mobility shift assay (EMSA). Results Here we show that low concentration of 1, 25-dihydroxyvitamin D3 links to can-cerization and significantly inhibits proliferation of undifferentiated gastric cancer cell lines SGC-7901 and BGC-823. BMP3 promoter hypermethylation was highly correlated with gastric tumor. Moreover, BMP3 expression was regulated by its promoter methylation in gastric cells. The further exploration of the relationship between 1, 25-dihydroxyvitamin D3 and BMP3 by EMSA results that 1, 25-dihydroxyvitamin D3 stimulates BMP3 expression by the inhibition of BMP3 promoter methylation in gastric tumor cells. Conclusion In combination with the data from clinical research, bioinformatics analysis and experimental verification, we propose that 1, 25-hydroxylvitamin D3 affects gastric cancer progression by repressing BMP3 promoter methylation.
Published: 2019

13. Soluble Nogo receptor 1 fusion protein protects neural progenitor cells in rats with ischemic stroke

Author: Bao-Qi Yu, Wei You, Gen Ye, Hai-Wei He, Kwok-Fai So, Xin Li, and Yuelin Zhang
Subjects: 0301 basic medicine, proliferation, Central nervous system, Morris water navigation task, Endogeny, neural progenitor cells, Pharmacology, neurological function, lcsh:RC346-429, 03 medical and health sciences, 0302 clinical medicine, Developmental Neuroscience, Medicine, Progenitor cell, nerve regeneration, lcsh:Neurology. Diseases of the nervous system, business.industry, Nogo Receptor 1, neural regeneration, Nogo-66 receptor, Nogo66 receptor-Fc protein, differentiation, stroke, photothrombotic cortical injury, transplantation, Embryonic stem cell, Neural stem cell, Transplantation, 030104 developmental biology, medicine.anatomical_structure, business, 030217 neurology & neurosurgery, Research Article
Abstract: Soluble Nogo66 receptor-Fc protein (sNgR-Fc) enhances axonal regeneration following central nervous system injury. However, the underlying mechanisms remain unclear. In this study, we investigated the effects of sNgR-Fc on the proliferation and differentiation of neural progenitor cells. The photothrombotic cortical injury model of ischemic stroke was produced in the parietal cortex of Sprague-Dawley rats. The rats with photothrombotic cortical injury were randomized to receive infusion of 400 μg/kg sNgR-Fc (sNgR-Fc group) or an equal volume of phosphate-buffered saline (photothrombotic cortical injury group) into the lateral ventricle for 3 days. The effects of sNgR-Fc on the proliferation and differentiation of endogenous neural progenitor cells were examined using BrdU staining. Neurological function was evaluated with the Morris water maze test. To further examine the effects of sNgR-Fc treatment on neural progenitor cells, photothrombotic cortical injury was produced in another group of rats that received transplantation of neural progenitor cells from the hippocampus of embryonic Sprague-Dawley rats. The animals were then given an infusion of phosphate-buffered saline (neural progenitor cells group) or sNgR-Fc (sNgR-Fc + neural progenitor cells group) into the lateral ventricle for 3 days. sNgR-Fc enhanced the proliferation of cultured neural progenitor cells in vitro as well as that of endogenous neural progenitor cells in vivo, compared with phosphate-buffered saline, and it also induced the differentiation of neural progenitor cells into neurons. Compared with the photothrombotic cortical injury group, escape latency in the Morris water maze and neurological severity score were greatly reduced, and distance traveled in the target quadrant was considerably increased in the sNgR-Fc group, indicating a substantial improvement in neurological function. Furthermore, compared with phosphate-buffered saline infusion, sNgR-Fc infusion strikingly improved the survival and differentiation of grafted neural progenitor cells. Our findings show that sNgR-Fc regulates neural progenitor cell proliferation, migration and differentiation. Therefore, sNgR-Fc is a potential novel therapy for stroke and neurodegenerative diseases, The protocols were approved by the Committee on the Use of Live Animals in Teaching and Research of the University of Hong Kong (approval No. 4560-17) in November, 2015.
Published: 2019

14. A case of COVID-19 complicated by massive gastrointestinal bleeding

Author: Jin-Song Mu, Yan-Ling Wang, Zhang Wenhui, and Xiao-Bao Qi
Subjects: medicine.medical_specialty, Gastrointestinal bleeding, 2019-20 coronavirus outbreak, Coronavirus disease 2019 (COVID-19), business.industry, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Brief Report, MEDLINE, Gastroenterology, medicine.disease, medicine, Intensive care medicine, business, AcademicSubjects/MED00260
Published: 2020
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15. Combined the GABA-A and GABA-B receptor agonists attenuates autistic behaviors in a prenatal valproic acid-induced mouse model of autism

Author: Jian-Quan Yang, Chao-Hua Yang, and Bao-Qi Yin
Subjects: Agonist, Male, medicine.drug_class, Autism Spectrum Disorder, Prefrontal Cortex, Synaptic Transmission, 03 medical and health sciences, Behavioral Neuroscience, Mice, 0302 clinical medicine, Pregnancy, medicine, Animals, GABA-A Receptor Agonists, GABA-B Receptor Agonists, gamma-Aminobutyric Acid, 030304 developmental biology, 0303 health sciences, Valproic Acid, Behavior, Animal, GABAA receptor, business.industry, Synaptic Potentials, medicine.disease, Developmental disorder, Mice, Inbred C57BL, Disease Models, Animal, Autism spectrum disorder, Prenatal Exposure Delayed Effects, Autism, GABAergic, Anticonvulsants, Drug Therapy, Combination, Female, business, Neuroscience, 030217 neurology & neurosurgery, medicine.drug
Abstract: Autism spectrum disorder (ASD) is an immensely challenging developmental disorder characterized primarily by two core behavioral symptoms of social communication deficits and restricted/repetitive behaviors. Investigating the etiological process and identifying an appropriate therapeutic target remain as formidable challenges to overcome ASD due to numerous risk factors and complex symptoms associated with the disorder. Among the various mechanisms that contribute to ASD, the maintenance of excitation and inhibition balance emerged as a key factor to regulate proper functioning of neuronal circuitry. In this study, we employed prenatally exposed to valproic acid (VPA) to establish a validated ASD mouse model and found impaired inhibitory gamma-aminobutyric acid (GABAergic) neurotransmission through a presynaptic mechanism in these model mice, which was accompanied with decreased GABA release and GABA-A and GABA-B receptor subunits expression. And acute administration of individual GABA-A or GABA-B receptor agonists partially reversed autistic-like behaviors in the model mice. Furthermore, acute administration of the combined GABA-A and GABA-B receptor agonists palliated sociability deficits, anxiety and repetitive behaviors in the animal model of autistic-like behaviors, demonstrating the therapeutic potential of above cocktail in the treatment of ASD.
Published: 2020

16. Critical care admission following elective surgery was not associated with survival benefit

Author: Kahan, Brennan C., Desponia, Koulenti, Kostoula, Arvaniti, Vanessa, Beavis, Douglas, Campbell, Matthew, Chan, Rui, Moreno, Pearse, Rupert M., Scott, Beattie, Pierre-Alain, Clavien, Nicolas, Demartines, Lee, A Fleisher, Mike, Grocott, James, Haddow, Andreas, Hoeft, Peter, Holt, Naomi, Pritchard, Andrew, Rhodes, Duminda, Wijeysundera, Matt, Wilson, Tahania, Ahmed, Kirsty, Everingham, Russell, Hewson, Marta, Januszewska, Rupert, M Pearse, Mandeep-Kaur, Phull, Richard, Halliwell, Mark, Shulman, Paul, Myles, Werner, Schmid, Michael, Hiesmayr, Patrick, Wouters, Stefan de Hert, Suzana, Lobo, Xiangming, Fang, Lars, Rasmussen, Emmanuel, Futier, Matthieu, Biais, Aurélien, Venara, Karem, Slim, Michael, Sander, Despoina, Koulenti, Mathew, Chan, Atul, Kulkarni, Susilo, Chandra, Aida, Tantri, Emad, Geddoa, Muntadhar, Abbas, Giorgio Della Rocca, Datin, Sivasakthi, Marzida, Mansor, Pastor, Luna, Arthur, Bouwman, Wolfgang, Buhre, Tim, Short, Tunde, Osinaike, Ricardo, Matos, Ioana, Grigoras, Mikhail, Kirov, Denis, Protsenko, Bruce, Biccard, Cesar, Aldecoa, Michelle, Chew, Christoph, Hofer, Martin, Hubner, James, Ditai, Tamas, Szakmany, Lee, Fleisher, Marissa, Ferguson, Michael, Macmahon, Ritchie, Cherian, Helen, Currow, Kathirgamanathan, Kanathiban, David, Gillespie, Edward, Pathmanathan, Katherine, Phillips, Jenifer, Reynolds, Joanne, Rowley, Jeanene, Douglas, Ross, Kerridge, Sameer, Garg, Michael, Bennett, Megha, Jain, David, Alcock, Nico, Terblanche, Rochelle, Cotter, Kate, Leslie, Marcelle, Stewart, Nicolette, Zingerle, Antony, Clyde, Oliver, Hambidge, Adam, Rehak, Sharon, Cotterell, Wilson Binh Quan Huynh, Timothy, Mcculloch, Erez, Ben-Menachem, Thomas, Egan, Jennifer, Cope, Paul, Fellinger, Simone, Haselberger, Caroline, Holaubek, Paul, Lichtenegger, Florian, Scherz, Franz, Hoffer, Veronika, Cakova, Andreas, Eichwalder, Norbert, Fischbach, Reinhold, Klug, Elisabeth, Schneider, Martin, Vesely, Reinhart, Wickenhauser, Karl Gernot Grubmueller, Marion, Leitgeb, Friedrich, Lang, Nancy, Toro, Marlene, Bauer, Friedrich, Laengle, Thomas, Mayrhofer, Christian, Buerkle, Karin, Forstner, Reinhard, Germann, Harald, Rinoesl, Elke, Schindler, Ernst, Trampitsch, Gerhard, Fritsch, Christian, Szabo, Jawad, Bidgoli, Hans, Verdoodt, Patrice, Forget, David, Kahn, Fernande, Lois, Mona, Momeni, Caroline, Prégardien, Audrey, Pospiech, Arnaud, Steyaert, Laurent, Veevaete, Dirk De Kegel, Karen De Jongh, Luc, Foubert, Carine, Smitz, Marcel, Vercauteren, Jan, Poelaert, Veerle Van Mossevelde, Jacques, Abeloos, Stefaan, Bouchez, Marc, Coppens, Luc De Baerdemaeker, Isabel, Deblaere, Ann De Bruyne, Stefan De Hert, Kristine, Fonck, Bjorn, Heyse, Tom, Jacobs, Koen, Lapage, Anneliese, Moerman, Martine, Neckebroek, Aliaksandra, Parashchanka, Nathalie, Roels, Nancy Van Den Eynde, Michael, Vandenheuvel, Jurgen Van Limmen, Ann, Vanluchene, Caroline, Vanpeteghem, Piet, Wyffels, Christel, Huygens, Punitha, Vandenbempt, Marc Van de Velde, Dimitri, Dylst, Bruno, Janssen, Evelien, Schreurs, Fábia Berganton Aleixo, Keulle, Candido, Hugo Dias Batista, Mario, Guimarães, Jaqueline, Guizeline, João, Hoffmann, Suzana, M Lobo, Francisco Ricardo Lobo, Vinícius, Nascimento, Katia, Nishiyama, Lucas, Pazetto, Daniela, Souza, Rodrigo Souza Rodrigues, Ana Maria Vilela Dos Santos, Jaquelline, Jardim, Joao, Silva, Paulo do Nascimento Junior, Thalissa Hermínia Baio, Gabriel Isaac Pereira de Castro, Henri Roger Watanabe Oliveira, Cristina Prata Amendola, Gutemberg, Cardoso, Daniela, Ortega, Ana Flavia Brotto, Mirella Cristine De Oliveira, Álvaro, Réa-Neto, Fernando, Dias, Pedro, Azambuja, Marcos Freitas Knibel, Antonio, Martins, William, Almeida, Calim Neder Neto, Maria Angela Tardelli, Eliana, Caser, Marcio, Machado, Crisitiano, Aguzzoli, Sérgio, Baldisserotto, Fernanda Beck Tabajara, Fernanda, Bettega, La Hore Correa Rodrigues Júnior, Julia de Gasperi, Lais, Faina, Marcos Farias Nolasco, Bruna Ferreira da Costa Fischer, Mariana Fosch de Campos Ferreira, Cristina, Hartmann, Marta, Kliemann, Gustavo Luis Hubert Ribeiro, Julia Merladete Fraga, Thiago Motta Netto, Laura Valduga Pozza, Paulo Rafael Wendling, Caroline, Azevedo, Juliana, Garcia, Marcel, Lopes, Bernardo, Maia, Paula, Maselli, Ralph, Melo, Weslley, Mendes, Matheus, Neves, Jacqueline, Ney, Claudio, Piras, Christopher, Applewhaite, Adrienne, Carr, Lorraine, Chow, Kaylene, Duttchen, Julena, Foglia, Michael, Greene, Ashley, Hinther, Kendra, Houston, Thomas Jared McCormick, Jennifer, Mikhayel, Sam, Montasser, Alex, Ragan, Andrew, Suen, Adrianna, Woolsey, Hai Chuan Yu, Duane, Funk, Stephen, Kowalski, Regina, Legaspi, Heather, Mcdonald, Faisal, Siddiqui, Jeremy, Pridham, Bernadette, Rowe, Sonia, Sampson, Barton, Thiessen, Geoff, Zbitnew, Andre, Bernard, Ronald, George, Philip, Jones, Rita, Moor, Naveed, Siddiqui, Alexandra, Wolfer, Diem, Tran, Denyse, Winch, Gary, Dobson, Thomas, Mccormick, Osama, Montasser, Richard, Hall, Leyla, Baghirzada, Si Yuan Dai, Gregory, Hare, Esther, Lee, Uma, Shastri, Albert, Tsui, Anmol, Yagnik, Danielle, Alvares, Stephen, Choi, Heather, Dwyer, Kathrina, Flores, Colin, Mccartney, Priya, Somascanthan, Carroll, Jo, Janneth, Pazmino-Canizares, Noam, Ami, Vincent, Chan, Anahi, Perlas, Ruth, Argue, Katie, Lavis, Kelly, Mayson, Ying, Cao, Hong, Gao, Tingju, Hu, Jie, Lv, Jian, Yang, Yang, Yang, Zhong, Yi, Jing, Zhou, Xiaohua, Zou, Miao, He, Xiaoying, Li, Dihuan, Luo, Haiying, Wang, Tian, Yu, Liyong, Chen, Lijun, Wang, Yunfei, Cai, Zhongming, Cao, Yanling, Li, Jiaxin, Lian, Haiyun, Sun, Sheng, Wang, Zhipeng, Wang, Kenru, Wang, Zhu, Yi, Xindan, Du, Hao, Fan, Yunbin, Fu, Lixia, Huang, Yanming, Huang, Haifang, Hwan, Hong, Luo, Pi-Sheng, Qu, Fan, Tao, Zhen, Wang, Guoxiang, Wang, Shun, Wang, Yan, Zhang, Xiaolin, Zhang, Chao, Chen, Weixing, Wang, Zhengyuan, Liu, Lihua, Fan, Jing, Tang, Yijun, Chen, Yongjie, Chen, Yangyang, Han, Changshun, Huang, Guojin, Liang, Jing, Shen, Jun, Wang, Qiuhong, Yang, Jungang, Zhen, Haidong, Zhou, Junping, Chen, Zhang, Chen, Xiaoyu, Li, 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Bhavika, Daya, Leanne, Drummond, Ali, Elabib, Ehab Helmy Abdel Goad, Ismail, E Goga, Riaz, Goga, Harrichandparsad, R, Richard, E Hodgson, Jordaan, J, Nicky, Kalafatis, Christian, Kampik, T Landers, A, Emil, Loots, Rajhmum, Madansein, Anil, Madaree, Thandinkosi, E Madiba, Vukani, T Manzini, Mbali, Mbuyisa, Rajan, Moodley, Mduduzi, Msomi, Innocent, Mukama, Desigan, Naidoo, Rubeshan, Naidoo, Tesuven, K Naidu, Sindiswa, Ntloko, Eneshia, Padayachee, Lucelle, Padayachee, Martijn, Phaff, Bala, Pillay, Desigan, Pillay, Lutchmee, Pillay, Anupa, Ramnarain, Suren, R Ramphal, Paul, Ryan, Ahmed, Saloojee, Motshedisi, Sebitloane, Noluyolo, Sigcu, Jenna, L Taylor, Alexandra, Torborg, Linda, Visser, Philip, Anderson, Alae, Conradie, Mathew de Swardt, Martin de Villiers, Johan, Eikman, Riaan, Liebenberg, Johan, Mouton, Abbey, Paton, Louwrence van der Merwe, Candice, Wilscott-Davids, Wendy Joan Barrett, Marlet, Bester, Johan de Beer, Jacques, Geldenhuys, Hanni, Gouws, Jan-Hendrik, Potgieter, Magdel, Strydom, Edwin, Wilberforce-Turton, Rubendraj, R Chetty, Subash, Chirkut, Larissa, Cronje, Kim de Vasconcellos, Nokukhanya, Z Dube, N Sibusiso Gama, Garyth, M Green, Randolph, Green-Thompson, Suman Mewa Kinoo, Prenolin, Kistnasami, Kapil, Maharaj, Manogaran, S Moodley, Sibongile, J Mothae, Ruvashni, Naidoo, Aslam, M, Noorbhai, F, Vivesh, Rughubar, Jenendhiran, Reddy, Avesh, Singh, David, L Skinner, Murray, J Smith, Bhagwan, Singh, Ravi, Misra, Maheshwar, Naidoo, Pireshin, Ramdharee, Yvonne, Selibea, Selina, Sewpersad, Shailendra, Sham, Joseph, D Wessels, Cucu, Africander, Tarek, Bejia, Stephen, P Blakemore, Marisa, Botes, Bimalshakth, Bunwarie, Carlos, B Hernandez, Mohammud, A Jeeraz, Dagmara, A Legutko, Acela, G Lopez, Jenine, N De Meyer, Tanaka, Muzenda, Noel, Naidoo, Maryam, Patel, Rao, Pentela, Marina, Junge, Naj, Mansoor, Lana, Rademan, Pawel, Scislowski, Ismail, Seedat, Bianca van den Berg, Doreen van der Merwe, Steyn van Wyk, Komalan, Govender, Darshan, Naicker, Rajesh, Ramjee, 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Patricia, Salgado, Luis, Suarez, Alejandro, Suarez-de-la-Rica, María José Villagrán, José Ignacio Alonso, Estefania, Cabezuelo, Irene, Garcia-Saiz, Olga Lopez Del Moral, Silvia, Martín, Alba Perez Gonzalez, Sherezade Tovar Doncel, Martin Agüero Vera, Francisco José Ávila Sánchez, Beatriz, Castaño, Beatriz Castaño Moreira, Sahely Flores Risco, Daniel Paz Martín, Fernando Pérez Martín, Paloma, Poza, Adela, Ruiz, Wilson Fabio Serna Martínez, Bárbara Vázquez Vicente, Saul Velaz Dominguez, Salvador, Fernández, Alfonso, Munoz-López, Maria Jose Bernat, Arantxa, Mas, Kenneth, Planas, Monir, Jawad, Yousif, Saeed, Annika, Hedin, Helena, Levander, Sandra, Holmström, David, Lönn, Frank, Zoerner, Irene, Åkring, Carl, Widmark, Jan, Zettergren, Victor Aspelund Liljequist, Lena, Nystrom, Suzanne, Odeberg-Wernerman, Anders, Oldner, Patrik, Reje, Sara, Lyckner, Jesper, Sperber, Anne, Adolfsson, Bengt, Klarin, Katrin, Ögren, Jean-Pierre, Barras, Thomas, Bührer, Vasileios, Despotidis, Naeder, Helmy, 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Davies, Matthew, Martin, Sian, Thomas, Karen, Burns, Carol, Mcarthur, Panna, Patel, Gary, Lau, Natalie, Rich, Fiona, Davis, Rachel, Lyons, Beth, Port, Rachel, Prout, Christopher, Smith, Yemi, Adelaja, Victoria, Bennett, Heena, Bidd, Alexandra, Dumitrescu, Jacqui Fox Murphy, Abigail, Keen, Nhlanhla, Mguni, Cheng, Ong, George, Adams, Piers, Boshier, Richard, Brown, Izabella, Butryn, Jayanta, Chatterjee, Alexander, Freethy, Geoffrey, Lockwood, Maria, Tsakok, Sophia, Tsiligiannis, William, Peat, Lorraine, Stephenson, Mike, Bradburn, Sara, Pick, Pedro, Cunha, Olufemi, Olagbaiye, Salim, Tayeh, Caroline, Abernethy, Madhu, Balasubramaniam, Rachael, Bennett, David, Bolton, Victoria, Martinson, Charde, Naylor, Stephanie, Bell, Blaylock, Heather, Vlad, Kushakovsky, Liam, Alcock, Hazel, Alexander, Colette, Anderson, Paul, Baker, Morag, Brookes, Louise, Cawthorn, Emanuel, Cirstea, Kerry, Colling, Ian, Coulter, Suparna, Das, Kathryn, Haigh, Alhafidz, Hamdan, Keith, Hugill, Lucksy, Kottam, Emily, 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Vivian, Emer, Berntsen, Tracey, Nolan, Angus, Turner, Akbar, Vohra, Andrew, Brown, Richard, Clark, Elaine, Coughlan, Conway, Daniel, Chinmay, Patvardhan, Rachel, Pearson, Sheba, Predeep, Hesham, Saad, Mohanakrishnan, Shanmugam, Simon, Varley, Katharine, Wylie, Lucy, Cooper, Arystarch, Makowski, Beata, Misztal, Eliza, Moldovan, Claire, Pegg, Andrew, Donovan, Jayne, Foot, Simon, Large, Andrew, Claxton, Bhagyashree, Netke, Richard, Armstrong, Claire, Calderwood, Andy, Kwok, Otto, Mohr, Peter, Oyeniyi, Lisa, Patnaik, Benjamin, Post, Sarah, Ali, Homa, Arshad, Gerard, Baker, Laura, Brenner, Maximilian, Brincat, Annemarie, Brunswicker, Hannah, Cox, Octavian Ionut Cozar, Alexander, Durst, Lior, Fengas, Jim, Flatt, Georgina, Glister, Vishal, Narwani, Evangelos, Photi, Adeline, Rankin, Melissa, Rosbergen, Mark, Tan, Ceri, Beaton, Rachel, Horn, Jane, Hunt, Guy, Rousseau, Lucia, Stancombe, Mohammed, Absar, Joanne, Allsop, Zoe, Drinkwater, Tracey, Hodgkiss, Kirsty, Smith, Jamie, Brown, Farhad, 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Woon, Yuan Liang, Abd Hadi, Husni Zaeem, Ahmad Azam, Ummi Azmira, Alias, Abdul Hafiz, Kesut, Saiful Aizar, Lee, Jun May, Ooi, Dar Vin, Sulaiman, Hetty Ayuni, Tengku Lih, Tengku Alini, Veerakumaran, Jeyaganesh, Rojas, Eder, Resendiz, Gerardo Esteban Alvarez, Zapata, Darcy Danitza Mari, Aguilar López, Julio Cesar Jesú, Flores, Armando Adolfo Alvarez, Amador, Juan Carlosc Bravo, Avila, Erendira Jocelin Dominguez, Aquino, Laura Patricia González, Rodriguez, Ricardo Lopez, Landa, Mariana Torre, Urias, Emma, Hollmann, Marku, Hulst, Abraham, Kirzner, Osne, Preckel, Benedikt, Koopman-van Gemert, Ankie, Buise, Marc, Tolenaar, Noortje, Weber, Eric, de Fretes, Jennifer, Houweling, Peter, Ormskerk, Patricia, Van Bommel, Jasper, Lance, Marcu, Smit-Fun, Valerie, van Zundert, Tom, Baas, Peter, de Boer, Hans Donald, Sprakel, Joost, Elferink-Vonk, Renske, Noordzij, Peter, van Zeggeren, Laura, Brand, Bastiaan, spanjersberg, Rob, ten Bokkel-Andela, Janneke, Numan, Janneke, van Klei, Wilton, van Zaane, 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Sarki, Adamu Muhammad, Adekola, Oyebola, Akanmu, Olanrewaju, Durodola, Akanmu, Olukoju, Olusegun, Raji, Victor, Olajumoke, Tokunbo, Oyebamiji, Emmanuel, Adenekan, Anthony, Adetoye, Adenekan, Faponle, Folayemi, Olateju, Simeon, Owojuyigbe, Afolabi, Talabi, Ademola, Adenike, Odewabi, Adewale, Badru, Collins, Nwokoro, Ezekiel, Emmanuel, Fatungase, Oluwabunmi Motunrayo, Grace, Anuforo, Sola, Sotannde, Stella, Ogunmuyiwa, Ademola, Adeyinka, Adeolu, Augustine A., Adigun, Tinuolac, Akinwale, Mukaila, Fasina, Oluyemi, Gbolahan, Olalere, Idowu, Olusola, Olonisakin, Rotimi Peter, Osinaike, Babatunde Babasola, Asudo, Felicia, Mshelia, Danladi, Abdur-Rahman, Lukman, Agodirin, Olayide, Bello, Jibril, Bolaji, Benjamin, Oyedepo, Olanrewaju Olubukola, Ezike, Humphrey, Iloabachie, Ikechukwu, Okonkwo, Ikemefuna, Onuora, Elia, Onyeka, Tonia, Ugwu, Innocent, Umeh, Friday, Alagbe-Briggs, Olubusola, Dodiyi-Manuel, Amabra, Echem, Richard, Obasuyi, Bright, Onajin-Obembe, Bisola, Bandeira, Maria Expedito, Martins, Alda, Tomé, Miguel, Martins Costa, Ana Cristina Miranda, Krystopchuk, Andriy, Branco, Teresa, Esteves, Simao, Melo, Marco António, Monte, Júlia, Rua, Fernando, Martins, Isabel, Pinho-Oliveira, Vítor Miguel, Rodrigues, Carla Maria, Cabral, Raquel, Marques, Sofia, Rêgo, Sara, Teixeira Jesus, Joana Sofia, Conceição Marques, Maria, Romao, Cristina, Dias, Sandra, Santos, Ana Margarida, Alves, Maria Joao, Salta, Cristina, Cruz, Salome, Duarte, Célia, Furtado Paiva, António Armando, do Nascimento Cabral, Tiago, Fariae Maia, Dionisio, Correia da Silva, Rui Freitas Mendonça, Langner, Anuschka, Oliveira Resendes, Hernâni, da Conceição Soares, Maria, Abrunhosa, Alexandra, Faria, Filomena, Miranda, Lina, Pereira, Helena, Serra, Sofia, Ionescu, Daniela, Margarit, Simona, Mitre, Calin, Vasian, Horatiu, Manga, Gratiela, Stefan, Andreea, Tomescu, Dana, Filipescu, Daniela, Paunescu, Marilena-Alina, Stefan, Mihai, Stoica, Radu, Gavril, Laura, Pătrăşcanu, Emilia, Ristescu, Irina, Rusu, Daniel, Diaconescu, Ciresica, Iosep, Gabriel Florin, Pulbere, Dorin, Ursu, Irina, Balanescu, Andreea, Grintescu, Ioana, Mirea, Liliana, Rentea, Irina, Vartic, Mihaela, Lupu, Mary-Nicoleta, Stanescu, Dorin, Streanga, Lavinea, Antal, Oana, Hagau, Natalia, Patras, Dumitru, Petrisor, Cristina, Tosa, Flaviu, Tranca, Sebastian, Copotoiu, Sanda Maria, Ungureanu, Liviu Lucian, Harsan, Cristian Remu, Papurica, Mariu, Cernea, Daniela Denisa, Dragoescu, Nicoleta Alice, Aflori, Laura, Vaida, Carmen, Ciobotaru, Oana Roxana, Aignatoaie, Mariana, Carp, Cristina Paula, Cobzaru, Isabelle, Mardare, Oana, Purcarin, Bianca, Tutunaru, Valentin, Ionita, Victor, Arustei, Mirela, Codita, Anisoara, Busuioc, Mihai, Chilinciuc, Ion, Ciobanu, Cristina, Belciu, Ioana, Tincu, Eugen, Blaj, Mihaela, Grosu, Ramona-Mihaela, Sandu, Gigel, Bruma, Dana, Corneci, Dan, Dutu, Madalina, Krepil, Adriana, Copaciu, Elena, Dumitrascu, Clementina Oana, Jemna, Ramona, Mihaescu, Florentina, Petre, Raluca, Tudor, Cristina, Ursache, Elena, Kulikov, Alexander, Lubnin, Andrey, Grigoryev, Evgeny, Pugachev, Stanislav, Tolmasov, Alexander, Hussain, Ayyaz, Ilyina, Yana, Roshchina, Anna, Iurin, Aleksandr, Chazova, Elena, Dunay, Artem, Karelov, Alexey, Khvedelidze, Irina, Voldaeva, Olga, Belskiy, Vladislav, Dzhamullaev, Parvin, Grishkowez, Elena, Kretov, Vladimir, Levin, Valeriy, Molkov, Aleksandr, Puzanov, Sergey, Samoilenko, Aleksandr, Tchekulaev, Aleksandr, Tulupova, Valentina, Utkin, Ivan, Allorto, Nikki Leigh, Bishop, David Gray, Builu, Pierre Monji, Cairns, Carel, Dasrath, Ashish, de Wet, Jacque, den Hoedt, Marielle, Grey, Ben, Hayes, Morgan Philip, Küsel, Belinda Senta, Shangase, Nomcebo, Wise, Robert, Cacala, Sharon, Farina, Zane, Govindasamy, Vishendran, Kruse, Carl-Heinz, Lee, Carolyn, Marais, Leonard, Naidoo, Thinagrin Dhasarthun, Rajah, Chantal, Rodseth, Reitze Nil, Ryan, Lisa, von Rhaden, Richard, Adam, Suwayba, Alphonsus, Christella, Ameer, Yusuf, Anderson, Frank, Basanth, Sujith, Bechan, Sudha, Bhula, Chettan, 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Delgado García, David R., Zamudio, Diana, Garcia Del Valle, Santiago, Luz Serrano, M., Alonso, Eduardo, Anillo, Victor, Maseda, Emilio, Salgado, Patricia, Suarez, Lui, Suarez-de-la-Rica, Alejandro, Villagrán, María José, Alonso, José Ignacio, Cabezuelo, Estefania, Garcia-Saiz, Irene, Lopez del Moral, Olga, Martín, Silvia, Perez Gonzalez, Alba, Tovar Doncel, Sherezade, Vera, Martin Agüero, Ávila Sánchez, Francisco José, Castaño, Beatriz, Castaño Moreira, Beatriz, Flores Risco, Sahely, Paz Martín, Daniel, Pérez Martín, Fernando, Poza, Paloma, Ruiz, Adela, Serna Martínez, Wilson Fabio, Vicente, Bárbara Vázquez, Dominguez, Saul Velaz, Fernández, Salvador, Munoz-López, Alfonso, Bernat, Maria Jose, Mas, Arantxa, Planas, Kenneth, Jawad, Monir, Saeed, Yousif, Hedin, Annika, Levander, Helena, Holmström, Sandra, Lönn, David, Zoerner, Frank, Åkring, Irene, Widmark, Carl, Zettergren, Jan, Liljequist, Victor Aspelund, Nystrom, Lena, Odeberg-Wernerman, Suzanne, Oldner, Ander, Reje, Patrik, Lyckner, 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Bottrill, Fiona, Ramalingam, Ganesh, Webb, Stephen, Akerman, Nik, Antill, Philip, Bourner, Lynsey, Buckley, Sarah, Castle, Gail, Charles, Rob, Eggleston, Christopher, Foster, Rebecca, Gill, Satwant, Lindley, Kate, Lklouk, Mohamed, Lowery, Tracey, Martin, Oliver, Milne, David, O’Connor, Patrick, Ratcliffe, Andrew, Rose, Alastair, Smith, Annie, Varma, Sandeep, Ward, Jackie, Barcraft-Barnes, Helena, Camsooksai, Julie, Colvin, Carolyn, Reschreiter, Henrik, Tbaily, Lee, Venner, Nicola, Hamilton, Caroline, Kelly, Lewi, Toth-Tarsoly, Piroska, Dodsworth, Kerry, Foord, Denise, Gordon, Paul, Hawes, Elizabeth, Lamb, Nikki, Mouland, Johanna, Nightingale, Jeremy, Rose, Steve, Schrieber, Joe, Al ‘Amri, Khalid, Aladin, Hafiz, Arshad, Mohammed Asif, Barraclough, Jame, Bentley, Conor, Bergin, Colin, Carrera, Ronald, Clarkson, Aisling, Collins, Michelle, Cooper, Lauren, Denham, Samuel, Griffiths, Ewen, Ip, Peter, Jeyanthan, Somasundaram, Joory, Kavita, Kaur, Satwant, Marriott, Paul, Mitchell, Natalie, Nagaiah, Sukumar, Nilsson, Annette, Parekh, Nilesh, Pope, Martin, Seager, Joseph, Serag, Hosam, Tameem, Alifia, Thomas, Anna, Thunder, Joanne, Torrance, Andrew, Vohra, Ravinder, whitehouse, Arlo, Wong, Tony, Blunt, Mark, Wong, Kate, Giles, Julian, Reed, Isabelle, Weller, Debbie, Bell, Gillian, Birch, Julie, Damant, Rose, Maiden, Jane, Mewies, Clare, Prince, Claire, Radford, Jane, Balain, Birender, Banerjee, Robin, Barnett, Andrew, Burston, Ben, Davies, Kirsty, Edwards, Jayne, Evans, Chri, Ford, David, Gallacher, Pete, Hill, Simon, Jaffray, David, Karlakki, Sudheer, Kelly, Cormac, Kennedy, Julia, Kiely, Nigel, Lewthwaite, Simon, Marquis, Chri, Ockendon, Matthew, Phillips, Stephen, Pickard, Simon, Richardson, Jame, Roach, Richard, Smith, Tony, Spencer-Jones, Richard, Steele, Niall, Steen, Julie, Van Liefland, Marck, White, Steve, Faulds, Matthew, Harris, Meredyth, Kelly, Carrie, Nicol, Scott, Pearson, Sally Anne, Chukkambotla, Srikanth, Andrew, Alyson, Attrill, Elizabeth, Campbell, Graham, Datson, Amanda, Fouracres, Anna, Graterol, Juan, Graves, Lynne, Hong, Bosun, Ishimaru, Alexander, Karthikeyan, Arvind, King, Helen, Lawson, Tom, Lee, Gregory, Lyons, Saoirse, Macalister Hall, Andrew, Mathoulin, Sophie, Mcintyre, Eilidh, Mclaughlin, Danny, Mulcahy, Kathleen, Ratcliffe, Anna, Robbins, Jame, Sung, Weilin, Tayo, Adeoluwa, Trembath, Lisa, Venugopal, Suneetha, Walker, Robert, Wigmore, Geoffrey, Boereboom, Catherine, Downes, Charlotte, Humphries, Ryan, Melbourne, Susan, Smith, Coral, Tou, Samson, Ullah, Shafa, Batchelor, Nick, Boxall, Leigh, Broomby, Rupert, Deen, Tariq, Hellewell, Alistair, Helliwell, Laurence, Hutchings, Melanie, Hutchins, David, Keenan, Samantha, Mackie, Donna, Donna, Alison, Smith, France, Stone, Lucy, Thorpe, Kevin, Wassall, Richard, Woodgate, Andrew, Baillie, Shelley, Campbell, Tara, James, Sarah, King, Chri, Marques de Araujo, Daniela, Martin, Daniel, Morkane, Clare, Neely, Julia, Rajendram, Rajkumar, Burton, Megan, James, Kathryn, Keevil, Edward, Minik, Orsolya, Morgan, Jenna, Musgrave, Anna, Rajanna, Harish, Roberts, Tracey, Adamson, Michael, Jumbe, Sandra, Kendall, Jennie, Muthuswamy, Mohan Babu, Anderson, Charlotte, Cruikshanks, Andrew, Wrench, Ian, Zeidan, Lisa, Ardern, Diane, Harris, Benjamin, Hellstrom, Johanna, Martin, Jane, Thomas, Richard, Varsani, Nimu, Wrey Brown, Caroline, Docherty, Philip, Gillies, Michael, McGregor, Euan, Usher, Helen, Craig, Jayne, Smith, Andrew, Ahmad, Tahania, Bodger, Phoebe, Creary, Thai, Fowler, Alexander, Hewson, Ru, Ijuo, Eke, Jones, Timothy, Kantsedikas, Ilya, Lahiri, Sumitra, McLean, Aaron Lawson, Niebrzegowska, Edyta, Phull, Mandeep, Wang, Difei, Wickboldt, Nadine, Baldwin, Jacqueline, Doyle, Donna, Mcmullan, Sean, Oladapo, Michelle, Owen, Thoma, Williams, Alexandra, Daniel, Hull, Gregory, Peter, Husain, Tauqeer, Kirk-Bayley, Justin, Mathers, Edward, Montague, Laura, White, Stuart, Avis, Joanne, Cook, Tim, Dali-Kemmery, Lola, Kerslake, Ian, Lambourne, Victoria, Pearson, Annabel, Boyd, Christine, Callaghan, Mark, Lawson, Cathy, McCrossan, Roopa, Nesbitt, Vanessa, O’connor, Laura, Scott, Julia, Sinclair, Rhona, Farid, Nahla, Morgese, Ciro, Bhatia, Kailash, Karmarkar, Swati, Ahmed, Jamil, Branagan, Graham, Hutton, Monica, Swain, Andrew, Brookes, Jamie, Cornell, Jonathan, Dolan, Rachael, Hulme, Jonathan, Jansen van Vuuren, Amanda, Jowitt, Tom, Kalashetty, Gunasheela, Lloyd, Fran, Patel, Kiran, Sherwood, Nichola, Brown, Lynne, Chandler, Ben, Deighton, Kerry, Emma, Temlett, Haunch, Kirsty, Cheeseman, Michelle, Dent, Kathy, Garg, Sanjeev, Gray, Carol, Hood, Marion, Jones, Dawn, Juj, Joanne, Rao, Roshan, Walker, Tara, Al Anizi, Mashel, Cheah, Clarissa, Cheing, Yushio, Coutinho, Francisco, Gondo, Prisca, Hadebe, Bernard, Onie Hove, Mazvangu, khader, Ahamed, Krishnachetty, Bobby, Rhodes, Karen, Sokhi, Jagdish, Baker, Katie-Anne, Bertram, Wendy, Looseley, Alex, Mouton, Ronelle, Arnold, Glenn, Arya, Shobhit, Balfoussia, Danai, Baxter, Linden, Harris, Jame, Jones, Craig, Knaggs, Alison, Markar, Sheraz, Perera, Anisha, Scott, Alasdair, Shida, Asako, Sirha, Ravneet, Wright, Sally, Frost, Victoria, Gray, Catherine, MacGregor, Mark, Andrews, Emma, Arrandale, Lindsay, Barrett, Stephen, Cifra, Elna, Cooper, Mariese, Dragnea, Drago, Elna, Cifra, Maclean, Jennifer, Meier, Sonja, Milliken, Donald, Munns, Christopher, Ratanshi, Nadir, Salvana, Abegail, Watson, Anthony, Ali, Hani, Campbell, Gill, Critchley, Rebecca, Hicks, Catherine, Liddle, Alison, Pass, Marc, Ritchie, Charlotte, Thomas, Charlotte, Too, Lingxi, Welsh, Sarah, Gill, Talvinder, Johnson, Joanne, Reed, Joanne, Davis, Edward, Papadopoullos, Sam, Attwood, Clare, Biffen, Andrew, Boulton, Kerenza, Gray, Sophie, Hay, David, Mills, Sarah, Montgomery, Jane, Riddell, Rory, Simpson, Jame, Bhardwaj, Neeraj, Paul, Elaine, Uwubamwen, Nosakhare, Alexander, Maini, Arrich, Jame, Arumugam, Swarna, Blackwood, Dougla, Boggiano, Victoria, Brown, Robyn, Lam Chan, Yik, Chatterjee, Devnandan, Chhabra, Ashok, Christian, Rachel, Costelloe, Hannah, Coxwell Matthewman, Madeline, Dalton, Emma, Darko, Julia, Davari, Maria, Dave, Tejal, Deacon, Matthew, Deepak, Shantal, Edmond, Holly, Ellis, Jessica, El-Sayed, Ahmed, Eneje, Philip, English, Rose, Ewe, Renee, Foers, William, Franklin, John, Gallego, Laura, Garrett, Emily, Goldberg, Olivia, Goss, Harry, Greaves, Rosanna, Harris, Rudy, Hennings, Charle, Jones, Eleanor, Kamali, Nelson, Kokkinos, Naomi, Lewis, Cary, Lignos, Leda, Malgapo, Evaleen Victoria, Malik, Rizwana, Milne, Andrew, Mulligan, John-Patrick, Nicklin, Philippa, Palipane, Natasha, Parsons, Thoma, Piper, Rebecca, Prakash, Rohan, Ramesh, Byron, Rasip, Sarah, Reading, Jacob, Rela, Mariam, Reyes, Anna, Robert, Stephen, Rooms, Martin, Shah, Karishma, Simons, Henry, Solanki, Shalil, Spowart, Emma, Stevens, Amy, Thomas, Christopher, Waggett, Helena, Yassaee, Arrash, Kennedy, Anthony, Scott, Sara, Somanath, Sameer, Berg, Andrew, Hernandez, Miguel, Nanda, Rajesh, Tank, Ghanshyambhai, Wilson, Natalie, 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Tsang, M.S., Lai, M.L., Yip, C.P., Chan, HMH, Law, B., Li, W.S., Chu, H.M., Koo, EGY, Lam, CCJ, Cheng, K.H., Lam, T., Chu, S., Lam, W.Y., Wong, KWK, Kwok, D., Hung, CYJ, Chan, WKJ, LamWong, W., Chung, CKE, Ma, S.K., Kaushik, S., Shah, B., Shah, D., Shah, S., Ar, P., Muthuchellappan, R., Agarwal, V., Divatia, J., Mishra, S., Nimje, G., Pande, S., Savarkar, S., Shrivastava, A., Thomas, M., Yegnaram, S., Hidayatullah, R., Puar, N., Niman, S., Indra, I., Hamzah, Z., Yuliana, A., Abidin, U.N., Dursin, A.N., Kurnia, A., Susanti, A., Handayani, D., Aribawa, M.A., Arya, A., Senapathi, TGA, Utara, U.H., Wid, W.M., Wima, S., Wir, W.M., Jehosua, B., Kaunang, J., Lantang, E.Y., Najoan, R., Waworuntu, N., Awad, H., Fuad, A., Geddoa, B., Khalaf, A.R., Al Hussaini, S., Albaj, S., Kenber, M., Bettinelli, A., Spadaro, S., Volta, C.A., Giancarlo, L., Sottosanti, V., Spagnesi, L., Toretti, I., Alloj, C., Cardellino, S., Carmino, L., Costanzo, E., Fanfani, L.C., Novelli, M.T., Roasio, A., Bellandi, M., Bignami, E., Bocchino, S., Cabrini, L., Corti, D., Landoni, G., Meroni, R., Moizo, E., Monti, G., Pintaudi, M., Plumari, V.P., Taddeo, D., Testa, V., Winterton, D., Zangrillo, A., Cloro, L.M., Colangelo, C., Colangelo, A., Rotunno, G., Angel, M.P., Maria, C.P., Pata, A., Parrini, V., Gatta, A., Nastasi, M., Tinti, C., Arrigo, M., Benevento, A., Bottini, C., Cannavo', M., Gastaldi, C., Marchesi, A., Pascazio, A., Pata, F., Pozzi, E., Premoli, A., Tessera, G., Boschi, L., D'Andrea, R., Ghignone, F., Poggioli, G., Sibilio, A., Taffurelli, M., Ugolini, G., Ab Majid, M.A., Ab Rahman, R., Joseph, J., Pathan, F., Shah, MHS, Yap, H.L., Cheah, S., Chin, I.I., Looi, J.K., Tan, S.C., Visvalingam, S., Kwok, F.Y., Lee, C.K., Tan, T.S., Wong, S.M., Abdullah, N.H., Liew, C.F., Luxuman, L., Mohd Zin, N.H., Norddin, M.F., Alias, RLR, Wong, J.Y., Yong, J., Bin Mustapha, M.T., Chan, W.K., Dzulkipli, N., Kuan, P.X., Lee, Y.C., Alias, A., Guok, E.C., Jee, C.C., Ramon, B.R., Weng, C.W., Abd Ghafar, FNI, Aziz, F.Z., Hussain, N., Lee, H.S., Sukawi, I., Woon, Y.L., Abd Hadi, H.Z., Ahmad Azam, U.A., Alias, A.H., Kesut, S.A., Lee, J.M., Ooi, D.V., Sulaiman, H.A., Tengku Lih, T.A., Veerakumaran, J., Rojas, E., Resendiz, GEA, Zapata, DDM, Aguilar López, JCJ, Flores, AAA, Amador, JCB, Avila, EJD, Aquino, LPG, Rodriguez, R.L., Landa, M.T., Urias, E., Hollmann, M., Hulst, A., Kirzner, O., Preckel, B., Koopman-van Gemert, A., Buise, M., Tolenaar, N., Weber, E., de Fretes, J., Houweling, P., Ormskerk, P., Van Bommel, J., Lance, M., Smit-Fun, V., van Zundert, T., Baas, P., de Boer, H.D., Sprakel, J., Elferink-Vonk, R., Noordzij, P., van Zeggeren, L., Brand, B., Spanjersberg, R., Ten Bokkel-Andela, J., Numan, J., van Klei, W., van Zaane, B., Boer, C., van Duivenvoorde, Y., Hering, J.P., Zonneveldt, H., Hoare, S., Santa, S., Allen, S.J., Bell, R., Choi, H.D., Drake, M., Farrell, H., Higgie, K., Holmes, K., Jenkins, N., Kim, C.J., Kim, S., Law, K.C., McAllister, D., Park, K., Pedersen, K., Pfeifer, L., Salmond, T., Steynor, M., Tan, M., Waymouth, E., Ab Rahman, A.S., Armstrong, J., Dudson, R., Nilakant, J., Richard, S., Virdi, P., Dixon, L., Donohue, R., Farrow, M., Kennedy, R., Marissa, H., McKellow, M., Nicola, D., Pascoe, R., Roberts, S.J., Rowell, G., Sumner, M., Templer, P., Chandrasekharan, S., Fulton, G., Jammer, I., Ali, M., More, R., Wilson, L., Chang, Y.H., Chang, J., Fowler, C., Panckhurst, J., Sara, R., Stapelberg, F., Cherrett, V., Ganter, D.L., McCann, L., Foley, J., Gilmour, F., Lumsden, R., Moores, M., Olliff, S., Sardareva, E., Tai, J., Wikner, M., Wong, C., Chaddock, M., Czepanski, C., McKendry, P., Polakovic, D., Polakovich, D., Robert, A., Tormo Belda, M., Norton, T., Alherz, F., Barneto, L., Ramirez, A., Sayeed, A., Smith, N., Bennett, C., McQuoid, S., Jansen, T.L., Nico, Z., Scott, J., Freschini, D., Freschini, A., Hopkins, B., Manson, L., Stoltz, D., Bates, A., Davis, S., Freeman, V., McGaughran, L., Baskar Sharma, S., Burrows, T., Byrne, K., English, D., Johnson, R., Chai Law, K., Manikkam, B., Naidoo, S., Rumball, M., Whittle, N., Franks, R., Gibson-Lapsley, H., Salter, R., Walsh, D., Cooper, R., Perry, K., Obobolo, A., Sule, U.M., Ahmad, A., Atiku, M., Mohammed, A.D., Sarki, A.M., Adekola, O., Akanmu, O., Durodola, A., Olukoju, O., Raji, V., Olajumoke, T., Oyebamiji, E., Adenekan, A., Adetoye, A., Faponle, F., Olateju, S., Owojuyigbe, A., Talabi, A., Adenike, O., Adewale, B., Collins, N., Ezekiel, E., Fatungase, O.M., Grace, A., Sola, S., Stella, O., Ademola, A., Adeolu, A.A., Adigun, T., Akinwale, M., Fasina, O., Gbolahan, O., Idowu, O., Olonisakin, R.P., Osinaike, B.B., Asudo, F., Mshelia, D., Abdur-Rahman, L., Agodirin, O., Bello, J., Bolaji, B., Oyedepo, O.O., Ezike, H., Iloabachie, I., Okonkwo, I., Onuora, E., Onyeka, T., Ugwu, I., Umeh, F., Alagbe-Briggs, O., Dodiyi-Manuel, A., Echem, R., Obasuyi, B., Onajin-Obembe, B., Bandeira, M.E., Tomé, M., Martins Costa, ACM, Krystopchuk, A., Branco, T., Esteves, S., Melo, M.A., Monte, J., Rua, F., Martins, I., Pinho-Oliveira, V.M., Rodrigues, C.M., Cabral, R., Marques, S., Rêgo, S., Teixeira Jesus, J.S., Conceição Marques, M., Romao, C., Dias, S., Santos, A.M., Alves, M.J., Salta, C., Cruz, S., Duarte, C., Furtado Paiva, A.A., do Nascimento Cabral, T., Fariae Maia, D., Correia da Silva, RFM, Langner, A., Oliveira Resendes, H., da Conceição Soares, M., Abrunhosa, A., Faria, F., Miranda, L., Pereira, H., Serra, S., Ionescu, D., Margarit, S., Mitre, C., Vasian, H., Manga, G., Stefan, A., Tomescu, D., Filipescu, D., Paunescu, M.A., Stefan, M., Stoica, R., Gavril, L., Pătrăşcanu, E., Ristescu, I., Rusu, D., Diaconescu, C., Iosep, G.F., Pulbere, D., Ursu, I., Balanescu, A., Grintescu, I., Mirea, L., Rentea, I., Vartic, M., Lupu, M.N., Stanescu, D., Streanga, L., Antal, O., Hagau, N., Patras, D., Petrisor, C., Tosa, F., Tranca, S., Copotoiu, S.M., Ungureanu, L.L., Harsan, C.R., Papurica, M., Cernea, D.D., Dragoescu, N.A., Aflori, L., Vaida, C., Ciobotaru, O.R., Aignatoaie, M., Carp, C.P., Cobzaru, I., Mardare, O., Purcarin, B., Tutunaru, V., Ionita, V., Arustei, M., Codita, A., Busuioc, M., Chilinciuc, I., Ciobanu, C., Belciu, I., Tincu, E., Blaj, M., Grosu, R.M., Sandu, G., Bruma, D., Corneci, D., Dutu, M., Krepil, A., Copaciu, E., Dumitrascu, C.O., Jemna, R., Mihaescu, F., Petre, R., Tudor, C., Ursache, E., Kulikov, A., Lubnin, A., Grigoryev, E., Pugachev, S., Tolmasov, A., Hussain, A., Ilyina, Y., Roshchina, A., Iurin, A., Chazova, E., Dunay, A., Karelov, A., Khvedelidze, I., Voldaeva, O., Belskiy, V., Dzhamullaev, P., Grishkowez, E., Kretov, V., Levin, V., Molkov, A., Puzanov, S., Samoilenko, A., Tchekulaev, A., Tulupova, V., Utkin, I., Allorto, N.L., Bishop, D.G., Builu, P.M., Cairns, C., Dasrath, A., de Wet, J., den Hoedt, M., Grey, B., Hayes, M.P., Küsel, B.S., Shangase, N., Wise, R., Cacala, S., Farina, Z., Govindasamy, V., Kruse, C.H., Lee, C., Marais, L., Naidoo, T.D., Rajah, C., Rodseth, R.N., Ryan, L., von Rhaden, R., Adam, S., Alphonsus, C., 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Makowski, A., Misztal, B., Moldovan, E., Pegg, C., Donovan, A., Foot, J., Large, S., Claxton, A., Netke, B., Armstrong, R., Calderwood, C., Kwok, A., Mohr, O., Oyeniyi, P., Patnaik, L., Post, B., Ali, S., Arshad, H., Baker, G., Brenner, L., Brincat, M., Brunswicker, A., Cox, H., Cozar, O.I., Durst, A., Fengas, L., Flatt, J., Glister, G., Narwani, V., Photi, E., Rankin, A., Rosbergen, M., Beaton, C., Horn, R., Hunt, J., Rousseau, G., Stancombe, L., Absar, M., Allsop, J., Drinkwater, Z., Hodgkiss, T., Smith, K., Alexander-Sefre, F., Campey, L., Dudgeon, L., Hall, K., Hitchcock, R., James, L., Winstone, U., Ahmad, N., Bauchmuller, K., Harrison, J., Jeffery, H., Miller, D., Pinder, A., Pothuneedi, S., Rosser, J., Sanghera, S., Swift, D., Walker, R., Bester, D., Cavanagh, S., Cripps, H., Daniel, H., Lynch, J., Pyke, S., Scholefield, J., Whitworth, H., Bottrill, F., Ramalingam, G., Webb, S., Akerman, N., Antill, P., Bourner, L., Buckley, S., Castle, G., Charles, R., Eggleston, C., Foster, R., Gill, S., Lindley, K., Lklouk, M., Lowery, T., Martin, O., Milne, D., O'Connor, P., Ratcliffe, A., Rose, A., Smith, A., Varma, S., Ward, J., Barcraft-Barnes, H., Camsooksai, J., Colvin, C., Reschreiter, H., Tbaily, L., Venner, N., Hamilton, C., Kelly, L., Toth-Tarsoly, P., Dodsworth, K., Foord, D., Gordon, P., Hawes, E., Lamb, N., Mouland, J., Nightingale, J., Rose, S., Schrieber, J., Al 'Amri, K., Aladin, H., Arshad, M.A., Barraclough, J., Bentley, C., Bergin, C., Carrera, R., Clarkson, A., Collins, M., Denham, S., Griffiths, E., Ip, P., Jeyanthan, S., Joory, K., Kaur, S., Marriott, P., Mitchell, N., Nagaiah, S., Nilsson, A., Parekh, N., Pope, M., Seager, J., Serag, H., Tameem, A., Thomas, A., Thunder, J., Torrance, A., Vohra, R., Whitehouse, A., Wong, T., Blunt, M., Wong, K., Giles, J., Reed, I., Weller, D., Bell, G., Birch, J., Damant, R., Maiden, J., Mewies, C., Prince, C., Radford, J., Balain, B., Banerjee, R., Barnett, A., Burston, B., Edwards, J., Evans, C., Ford, D., Gallacher, P., Hill, S., Jaffray, D., Karlakki, S., Kennedy, J., Kiely, N., Lewthwaite, S., Marquis, C., Ockendon, M., Phillips, S., Pickard, S., Richardson, J., Roach, R., Smith, T., Spencer-Jones, R., Steele, N., Steen, J., Van Liefland, M., White, S., Faulds, M., Harris, M., Nicol, S., Pearson, S.A., Chukkambotla, S., Andrew, A., Attrill, E., Campbell, G., Datson, A., Fouracres, A., Graterol, J., Graves, L., Hong, B., Ishimaru, A., Karthikeyan, A., King, H., Lawson, T., Lee, G., Lyons, S., Macalister Hall, A., Mathoulin, S., Mcintyre, E., Mclaughlin, D., Mulcahy, K., Robbins, J., Sung, W., Tayo, A., Trembath, L., Venugopal, S., Wigmore, G., Boereboom, C., Downes, C., Humphries, R., Melbourne, S., Tou, S., Ullah, S., Batchelor, N., Boxall, L., Broomby, R., Deen, T., Hellewell, A., Helliwell, L., Hutchings, M., Hutchins, D., Keenan, S., Mackie, D., Donna, A., Smith, F., Stone, L., Thorpe, K., Wassall, R., Woodgate, A., Baillie, S., Campbell, T., James, S., King, C., Marques de Araujo, D., Martin, D., Morkane, C., Neely, J., Rajendram, R., Burton, M., James, K., Keevil, E., Minik, O., Morgan, J., Musgrave, A., Rajanna, H., Roberts, T., Adamson, M., Jumbe, S., Kendall, J., Muthuswamy, M.B., Cruikshanks, A., Wrench, I., Zeidan, L., Ardern, D., Harris, B., Hellstrom, J., Martin, J., Thomas, R., Varsani, N., Wrey Brown, C., Docherty, P., Gillies, M., McGregor, E., Usher, H., Craig, J., Ahmad, T., Bodger, P., Creary, T., Fowler, A., Ijuo, E., Jones, T., Kantsedikas, I., Lahiri, S., McLean, A.L., Niebrzegowska, E., Phull, M., Wickboldt, N., Baldwin, J., Doyle, D., Mcmullan, S., Oladapo, M., Owen, T., Williams, A., Gregory, P., Husain, T., Kirk-Bayley, J., Mathers, E., Montague, L., Avis, J., Cook, T., Dali-Kemmery, L., Kerslake, I., Lambourne, V., Pearson, A., Boyd, C., Callaghan, M., Lawson, C., McCrossan, R., Nesbitt, V., O'connor, L., Sinclair, R., Farid, N., Morgese, C., Bhatia, K., Karmarkar, S., Ahmed, J., Branagan, G., Hutton, M., Swain, A., Brookes, J., Cornell, J., Dolan, R., Hulme, J., Jansen van Vuuren, A., Jowitt, T., Kalashetty, G., Lloyd, F., Patel, K., Sherwood, N., Brown, L., Chandler, B., Deighton, K., Emma, T., Haunch, K., Cheeseman, M., Dent, K., Gray, C., Hood, M., Jones, D., Juj, J., Rao, R., Walker, T., Al Anizi, M., Cheah, C., Cheing, Y., Coutinho, F., Gondo, P., Hadebe, B., Onie Hove, M., Khader, A., Krishnachetty, B., Rhodes, K., Sokhi, J., Baker, K.A., Bertram, W., Looseley, A., Mouton, R., Arnold, G., Arya, S., Balfoussia, D., Baxter, L., Harris, J., Jones, C., Knaggs, A., Markar, S., Perera, A., Scott, A., Shida, A., Sirha, R., Wright, S., Frost, V., MacGregor, M., Andrews, E., Arrandale, L., Barrett, S., Cifra, E., Cooper, M., Dragnea, D., Elna, C., Maclean, J., Meier, S., Milliken, D., Munns, C., Ratanshi, N., Salvana, A., Watson, A., Ali, H., Critchley, R., Hicks, C., Liddle, A., Pass, M., Ritchie, C., Thomas, C., Too, L., Welsh, S., Gill, T., Johnson, J., Reed, J., Davis, E., Papadopoullos, S., Attwood, C., Biffen, A., Boulton, K., Gray, S., Hay, D., Mills, S., Montgomery, J., Riddell, R., Simpson, J., Bhardwaj, N., Paul, E., Uwubamwen, N., Alexander, M., Arrich, J., Arumugam, S., Blackwood, D., Boggiano, V., Lam Chan, Y., Chatterjee, D., Chhabra, A., Christian, R., Costelloe, H., Coxwell Matthewman, M., Dalton, E., Darko, J., Davari, M., Dave, T., Deacon, M., Deepak, S., Edmond, H., Ellis, J., El-Sayed, A., Eneje, P., English, R., Ewe, R., Foers, W., Franklin, J., Gallego, L., Garrett, E., Goldberg, O., Goss, H., Greaves, R., Harris, R., Hennings, C., Jones, E., Kamali, N., Kokkinos, N., Lewis, C., Lignos, L., Malgapo, E.V., Malik, R., Milne, A., Mulligan, J.P., Nicklin, P., Palipane, N., Parsons, T., Piper, R., Prakash, R., Ramesh, B., Rasip, S., Reading, J., Rela, M., Reyes, A., Robert, S., Rooms, M., Shah, K., Simons, H., Solanki, S., Spowart, E., Stevens, A., Waggett, H., Yassaee, A., Kennedy, A., Scott, S., Somanath, S., Berg, A., Hernandez, M., Nanda, R., Tank, G., Wilson, N., Wilson, D., Al-Soudaine, Y., Baldwin, M., Cornish, J., Davies, Z., Davies, L., Edwards, M., Frewer, N., Gallard, S., Glasbey, J., Harries, R., Hopkins, L., Kim, T., Koompirochana, V., Lawson, S., Lewis, M., Makzal, Z., Scourfield, S., Ahmad, Y., Bates, S., Blackwell, C., Bryant, H., Coulter, S., Cruickshank, R., Daniel, S., Daubeny, T., Golder, K., Hawkins, L., Helen, B., Hinxman, H., Levett, D., Skinner, B., Walsgrove, J., Dickson, J., Constantin, K., Karen, M., O'Brien, P., O'Donohoe, L., Payne, H., Sundayi, S., Walker, E., Brooke, J., Cardy, J., Humphreys, S., Kessack, L., Kubitzek, C., Kumar, S., Cotterill, D., Hodzovic, E., Hosdurga, G., Miles, E., Saunders, G., Campbell, M., Chan, P., Jemmett, K., Raj, A., Naik, A., Ramamoorthy, R., Shah, N., Sylvan, A., Blyth, K., Burtenshaw, A., Freeman, D., Johnson, E., Lo, P., Martin, T., Plunkett, E., Wollaston, J., Allison, J., Carroll, C., Craw, N., Craw, S., Pitt-Kerby, T., Rowland-Axe, R., Spurdle, K., McDonald, A., Simon, D., Sinha, V., Banner-Goodspeed, V., Boone, M., Campbell, K., Lu, F., Scannell, J., Sobol, J., Balajonda, N., Clemmons, K., Conde, C., Funk, B., Hopkins, T., Olaleye, O., Omer, O., Pender, M., Porto, A., Waweru, P., Yeh, E., Bodansky, D., Evans, A., Kleopoulos, S., Maril, R., Mathney, E., Sanchez, A., Tinuoye, E., Bateman, B., Eng, K., Jiang, N., Ladha, K., Needleman, J., Chen, L.L., Lane, R., Robinowitz, D., Ghushe, N., Irshad, M., Patel, S., Takemoto, S., Wallace, A., Mazzeffi, M., Rock, P., Wallace, K., Zhu, X., Chua, P., Mattera, M., Sharar, R., Thilen, S., Treggiari, M., Morgan, A., Sofjan, I., Subramaniam, K., Avidan, M., Maybrier, H., Muench, M., and Wildes, T.
Subjects: Male, HSJ UCI, Critical Care and Intensive Care Medicine, law.invention, Perioperative Care/methods, 0302 clinical medicine, Surgical procedures, 030202 anesthesiology, law, Critical care/utilisation, Postoperative care/methods, Postoperative care/statistics and numerical data, Surgical procedures, operative/mortality, statistics and numerical data, Prospective Studies, Postoperative Period, Postoperative care/methods, education.field_of_study, Postoperative care/method, Elective Surgical Procedures/mortality, fluid output, Middle Aged, fluid administration, Intensive care unit, Hospitalization, Length of Stay/statistics & numerical data, operative/mortality, Intensive Care Units/statistics & numerical data, outcome, Female, Elective Surgical Procedure, Human, Adult, medicine.medical_specialty, Logistic Model, Postoperative care/statistics and numerical data, Critical care/utilisation, Population, Intensive Care Unit, Elective Surgical Procedures/statistics & numerical data, Hospitalization/statistics & numerical data, Humans, Logistic Models, Surgical procedures, operative/mortality, Postoperative care, methods, Perioperative Care, NO, 03 medical and health sciences, Anesthesiology, Intensive care, medicine, Elective surgery, Intensive care medicine, education, business.industry, 030208 emergency & critical care medicine, Perioperative, Length of Stay, Clinical trial, Prospective Studie, septic shock, business
Abstract: PURPOSE: As global initiatives increase patient access to surgical treatments, there is a need to define optimal levels of perioperative care. Our aim was to describe the relationship between the provision and use of critical care resources and postoperative mortality. METHODS: Planned analysis of data collected during an international 7-day cohort study of adults undergoing elective in-patient surgery. We used risk-adjusted mixed-effects logistic regression models to evaluate the association between admission to critical care immediately after surgery and in-hospital mortality. We evaluated hospital-level associations between mortality and critical care admission immediately after surgery, critical care admission to treat life-threatening complications, and hospital provision of critical care beds. We evaluated the effect of national income using interaction tests. RESULTS: 44,814 patients from 474 hospitals in 27 countries were available for analysis. Death was more frequent amongst patients admitted directly to critical care after surgery (critical care: 103/4317 patients [2%], standard ward: 99/39,566 patients [0.3%]; adjusted OR 3.01 [2.10-5.21]; p < 0.001). This association may differ with national income (high income countries OR 2.50 vs. low and middle income countries OR 4.68; p = 0.07). At hospital level, there was no association between mortality and critical care admission directly after surgery (p = 0.26), critical care admission to treat complications (p = 0.33), or provision of critical care beds (p = 0.70). Findings of the hospital-level analyses were not affected by national income status. A sensitivity analysis including only high-risk patients yielded similar findings. CONCLUSIONS: We did not identify any survival benefit from critical care admission following surgery. info:eu-repo/semantics/publishedVersion
Published: 2017

17. miR-496/MMP10 Is Involved in the Proliferation of IL-1β-Induced Fibroblast-Like Synoviocytes Via Mediating the NF-κB Signaling Pathway

Author: Shu-Xin Feng, Bao-Qi Gong, Shiqing Feng, Xue-Wu Xing, Hong-Yu Shi, and Shen Liu
Subjects: 0301 basic medicine, Fibroblast-like synoviocyte, Cell Survival, Immunology, Interleukin-1beta, Flow cytometry, 03 medical and health sciences, 0302 clinical medicine, Matrix Metalloproteinase 10, medicine, Immunology and Allergy, Humans, Viability assay, Fibroblast, Cell Proliferation, Reporter gene, medicine.diagnostic_test, Cell growth, Chemistry, NF-kappa B, Fibroblasts, Synoviocytes, MicroRNAs, 030104 developmental biology, medicine.anatomical_structure, Apoptosis, 030220 oncology & carcinogenesis, Cancer research, Signal transduction, Signal Transduction
Abstract: Rheumatoid arthritis (RA) is a common chronic autoimmune disease featured by synovial inflammation. miR-496 is closely involved in various pathologic conditions. However, its role in RA has not yet been elucidated. Expression of miR-496 and MMP10 was determined based on the clinical samples with RA retrieved from the Gene Expression Omnibus (GEO) datasets. In vitro model of RA was constructed in MH7A cells stimulated by IL-1β (10 ng/mL). Cell counting kit 8 (CCK-8) and flow cytometry experiments were implemented to investigate the cell viability and apoptosis rate of MH7A cells. TargetScan was applied to identify the targets of miR-496, and the regulation of miR-496 on MMP10 expression was validated by a dual-luciferase reporter gene assay. qRT-PCR and western blot analyses were conducted to examine the expression. miR-496 expression was decreased in RA tissues and MH7A cells after IL-1β treatment. Overexpression of miR-496 significantly inhibited IL-1β-treated MH7A cell viability. MMP10 was identified as a target of miR-496 and its expression was negatively regulated by miR-496. The effects of miR-496 on MH7A cell proliferation and apoptosis were reversed by MMP10. The activity of NF-κB pathway was associated with the miR-496/MMP10 axis in IL-1β-stimulated MH7A cells. To summarize, this study demonstrated that miR-496 can impair the proliferative ability and facilitate the apoptosis of IL-1β-treated MH7A through regulating MMP10 expression and NF-κB signaling pathway.
Published: 2020

18. Using Alendronic Acid Coupled Fluorescently Labelled SM Liposomes as a Vehicle for Bone Targeting

Author: Wolfgang Baum, Timo Damm, Oula Peñate Medina, Tuula Peñate Medina, Jana Humbert, Olga Will, Bao Qi, and Claus C. Glüer
Subjects: Indocyanine Green, Biodistribution, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Drug Discovery, medicine, Humans, Tissue Distribution, Optical tomography, 030304 developmental biology, Fluorescent Dyes, Pharmacology, 0303 health sciences, Liposome, medicine.diagnostic_test, Alendronate, Chemistry, Alendronic acid, Fluorescence, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, Drug delivery, Liposomes, Sphingomyelin, Indocyanine green, Biomedical engineering, medicine.drug
Abstract: Background: We recently developed a liposomal nanoparticle system that can be used for drug delivery and simultaneously be monitored by optical or photoacoustic imaging devices. Here we tested the efficacy of alendronate as a homing molecule in SM-liposomes for bone targeting. Methods: Alendronate was immobilized covalently on the liposomal surface and the fluorescent dye indocyanine green was used as a payload in the liposomes. The indocyanine green delivery was analyzed by 3D optical tomography, optical fluorescence scanner, photoacoustic imaging, and by ex-vivo biodistribution studies. Results: The results show that the alendronate, coupled to the liposomal surface, increases sphingomyelin containing liposome targeting up to several-folds. Conclusion: The alendronate targeted liposomes open possibilities for an application in active bone targeting.
Published: 2020

19. Mitophagy is activated in brain damage induced by cerebral ischemia and reperfusion via the PINK1/Parkin/p62 signalling pathway

Author: Bao-Qi Wang, Yong Zhang, Tao Wu, Hai-Zhong Zheng, Ji-Tao Wu, Ya-Na Li, Yun-Zhi Ma, Chun-Qing Gu, Yan Wang, and Rui Lan
Subjects: Male, 0301 basic medicine, Time Factors, Ubiquitin-Protein Ligases, Ischemia, Gene Expression, PINK1, Brain damage, Mitochondrion, Parkin, Brain Ischemia, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, medicine.artery, Sequestosome-1 Protein, Mitophagy, Autophagy, medicine, Animals, Neurons, Kinase, Chemistry, General Neuroscience, Brain, Endothelial Cells, medicine.disease, Mitochondria, Cell biology, 030104 developmental biology, Astrocytes, Reperfusion Injury, Middle cerebral artery, medicine.symptom, Protein Kinases, 030217 neurology & neurosurgery, Signal Transduction
Abstract: This study examined the course of mitophagy following cerebral ischemia with reperfusion and the role of the PTEN-induced kinase 1 (PINK1)/Parkin/p62 signalling pathway. The middle cerebral artery of male Sprague-Dawley rats was occluded for 90 min and was followed by different time-points of reperfusion. Cerebral infarct areas were detected by 2,3,5-triphenyl tetrazolium chloride staining, while brain damage was observed by haematoxylin and eosin staining. Levels of LC3, Beclin1 and LAMP-1 were estimated by western blots. LC3B location was observed in various cells in the neurovascular unit. In addition, PINK1 accumulation in damaged mitochondria and Parkin/p62 mitochondrial translocation were investigated by double immunofluorescence staining. Finally, the levels of PINK1, Parkin and p62 expression in mitochondrial fractions were estimated by western blots. Cerebral ischemia with different time-points of reperfusion resulted in infarct in the territory of the middle cerebral artery accompanied by overall brain damage. In addition, we found up-regulation of LC3B, Beclin1, and LAMP-1, as well as mitophagy activation after reperfusion, with peak expression of these proteins at 24 h after reperfusion. Electron microscopy and immunofluorescence indicated that LC3B was primarily located in neurons, although lower levels of expression were found in astrocytes and even less in vascular endothelial cells. Moreover, significant increases in PINK1 accumulation in the outer membrane of mitochondria and increased Parkin/p62 mitochondrial translocation were shown at 24 h after reperfusion. These ﬁndings suggest that the PINK1/Parkin/p62 signalling pathway was involved in the pathophysiological processes following ischemia and reperfusion.
Published: 2018

20. Comprehensive analysis of key genes, microRNAs and long non‐coding RNAs in hepatocellular carcinoma

Author: Yu Zheng, Liang Wang, Wei Zhang, Lu-Meng Chao, Bao-Qi Shi, Yong-Sheng Tan, and Xue-Jun Zhang
Subjects: 0301 basic medicine, False discovery rate, mRNA, Computational biology, Biology, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0302 clinical medicine, Interaction network, microRNA, medicine, Research Articles, miRNA, Messenger RNA, Rnd3, Cytochrome P450, hepatocellular carcinoma, TCGA, medicine.disease, digestive system diseases, Long non-coding RNA, 030104 developmental biology, long non‐coding RNA, 030220 oncology & carcinogenesis, Hepatocellular carcinoma, biology.protein, Research Article
Abstract: Human hepatocellular carcinoma (HCC) is a common aggressive cancer whose molecular mechanism remains elusive. We aimed to identify the key genes, microRNAs (miRNAs) and long non‐coding RNAs (lncRNAs) involved with HCC. We obtained mRNA, miRNA and lncRNA profiles for HCC from The Cancer Genome Atlas and then identified differentially expressed mRNAs (DEmRNAs), miRNAs (DEmiRNAs) and lncRNAs (DElncRNAs). We performed functional annotation of DEmRNAs and then constructed HCC‐specific DEmiRNA–DEmRNA, DEmiRNA–DElncRNA and DElncRNA–DEmiRNA–DEmRNA interaction networks. We searched for nearby target cis‐DEmRNAs of DElncRNAs and performed receiver operating characteristic and survival analyses. A total of 1239 DEmRNAs, 33 DEmiRNAs and 167 DElncRNAs in HCC were obtained. Retinol metabolism [false discovery rate (FDR) = 7.02 × 10−14] and metabolism of xenobiotics by cytochrome P450 (FDR = 7.30 × 10−11) were two significantly enriched pathways in HCC. We obtained 545 DEmiRNA–DEmRNA pairs that consisted of 258 DEmRNAs and 28 DEmiRNAs in HCC. mir‐424, miR‐93 and miR‐3607 are three hub DEmiRNAs of the HCC‐specific DEmiRNA–DEmRNA interaction network. HAND2‐AS1/ENSG00000232855–miR‐93–LRAT/RND3, ENSG00000232855–miR‐877–RCAN1 and ENSG00000232855–miR‐224–RND3 interactions were found in the HCC‐specific DElncRNA–DEmiRNA–DEmRNA interaction network. A total of three DElncRNA–nearby target DEmRNA pairs (HCG25–KIFC1, LOC105378687–CDC20 and LOC101927043–EPCAM) in HCC were obtained. Diagnostic and prognostic values of several selected DElncRNAs, DEmRNAs and DEmiRNAs for HCC were assessed. Our study identified several DEmRNAs, DEmiRNAs and DElncRNAs with great diagnostic or prognostic value for HCC, which may facilitate studies into the molecular mechanisms, and development of potential biomarkers and therapeutic target sites for HCC.
Published: 2018

21. A new method of isolating spinal motor neurons from fetal mouse

Author: Yuzhong Wang, Liangchen Hu, Weifang Wang, Fei Wu, Wei Wang, Lulu Liu, Bao Qi, Hui Lv, Yanlei Hao, and Quanquan Wang
Subjects: 0301 basic medicine, Time Factors, Density gradient, Contrast Media, Cell Separation, Biology, Immunofluorescence staining, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Pregnancy, Fetal mouse, Triiodobenzoic Acids, Metrizamide, medicine, Animals, Cells, Cultured, Motor Neurons, General Neuroscience, Embryo, Motor neuron, Embryo, Mammalian, Spinal cord, 030104 developmental biology, medicine.anatomical_structure, Spinal Cord, nervous system, chemistry, Female, Neuroscience, 030217 neurology & neurosurgery
Abstract: Background Isolating of primary motor neurons from animal embryos is critical for the study of neurological disease including mechanistic discovery and therapeutic development. Density gradient centrifuge taking advantage of the buoyant of motor neuron permits the enrichment of motor neurons. Despite the metrizamide, an OptiPrep medium has been introduced to separate the motor neurons by gradient centrifuge. New method We hereby used single density gradient of OptiPrep medium to isolate the spinal motor neurons from the fetal mouse. Results Single density gradient of OptiPrep medium is effective to isolate spinal motor neurons from the fetal mouse. The immunofluorescence staining analysis showed that the purity of cultured motor neurons at 72 h was between 90% and 95%. Comparison with existing method Four gradients of OptiPrep medium have been previously used to isolate the motor neurons from spinal cord of mouse. In this study, the single gradient of OptiPrep medium was demonstrated to effectively isolate spinal motor neurons from the fetal mouse. Conclusions The single gradient of OptiPrep medium is enough to produce high purity of spinal motor neurons from the fetal mouse.
Published: 2017

22. Xiao-Xu-Ming Decoction Reduced Mitophagy Activation and Improved Mitochondrial Function in Cerebral Ischemia and Reperfusion Injury

Author: Tao Wu, Ya-Na Li, Hai-Zhong Zheng, Yun-Zhi Ma, Rui Lan, Ji-Tao Wu, Yan Wang, Bao-Qi Wang, Yong Zhang, and Chun-Qing Gu
Subjects: 0301 basic medicine, Male, Article Subject, Ischemia, H&E stain, Neurosciences. Biological psychiatry. Neuropsychiatry, Apoptosis, Pharmacology, Neuroprotection, Brain Ischemia, Rats, Sprague-Dawley, 03 medical and health sciences, symbols.namesake, Cyclosporin a, Mitophagy, Medicine, Animals, Medicine, Chinese Traditional, business.industry, Penumbra, General Medicine, medicine.disease, Mitochondria, Rats, Disease Models, Animal, 030104 developmental biology, Neuropsychology and Physiological Psychology, Neuroprotective Agents, Neurology, Reperfusion Injury, Nissl body, symbols, Neurology (clinical), business, Reperfusion injury, RC321-571, Research Article, Drugs, Chinese Herbal
Abstract: We investigated whether Xiao-Xu-Ming decoction reduced mitophagy activation and kept mitochondrial function in cerebral ischemia-reperfusion injury. Rats were randomly divided into 5 groups: sham, ischemia and reperfusion (IR), IR plus XXMD (60 g/kg/day) (XXMD60), IR plus cyclosporin A (10 mg/kg/day) (CsA), and IR plus vehicle (Vehicle). Focal cerebral ischemia and reperfusion models were induced by middle cerebral artery occlusion (MCAO). Cerebral infarct areas were measured by triphenyl tetrazolium chloride staining. Cerebral ischemic injury was evaluated by hematoxylin and eosin staining (HE) and Nissl staining. Ultrastructural features of mitochondria and mitophagy in the penumbra of the ischemic cortex were observed by transmission electron microscopy. Mitophagy was detected by immunofluorescence labeled with LC3B and VDAC1. Autophagy lysosome formation was observed by immunofluorescence labeled with LC3B and Lamp1. The expression of LC3B, Beclin1, and Lamp1 was analyzed by Western blot. The rats subjected to MCAO showed worsened neurological score and cell ischemic damage. These were all significantly reversed by XXMD or CsA. Moreover, XXMD/CsA notably downregulated mitophagy and reduced the increase in LC3, Beclin1, and Lamp1 expression induced by cerebral ischemia and reperfusion. The findings demonstrated that XXMD exerted neuroprotective effect via downregulating LC3, Beclin1, Lamp1, and mitochondrial p62 expression level, thus leading to the inhibition of mitophagy.
Published: 2018

23. Calenduloside E Analogues Protecting H9c2 Cardiomyocytes Against H2O2-Induced Apoptosis: Design, Synthesis and Biological Evaluation

Author: Yu Tian, Yu-Yang Du, Hai Shang, Min Wang, Zhong-Hao Sun, Bao-Qi Wang, Di Deng, Shan Wang, Xu-Dong Xu, Gui-Bo Sun, and Xiao-Bo Sun
Subjects: 0301 basic medicine, CALENDULOSIDE E, Poly ADP ribose polymerase, cardiomyocytes, Biology, medicine.disease_cause, 03 medical and health sciences, 0302 clinical medicine, Downregulation and upregulation, medicine, Pharmacology (medical), triterpenoid saponin, Original Research, Triterpenoid saponin, Biological evaluation, Pharmacology, chemistry.chemical_classification, Membrane potential, Calenduloside E (CE) analogues, lcsh:RM1-950, apoptosis, ROS, Cell biology, 030104 developmental biology, lcsh:Therapeutics. Pharmacology, chemistry, Biochemistry, Apoptosis, 030220 oncology & carcinogenesis, Oxidative stress, anti-apoptotic mechanism
Abstract: Modulation of apoptosis is therapeutically effective in cardiomyocytes damage. Calenduloside E (CE), a naturally occurring triterpenoid saponin, is a potent anti-apoptotic agent. However, little is known about its synthetic analogues on the protective effects in apoptosis of cardiomyocytes. The present research was performed to investigate the potential protective effect of CE analogues against H2O2-induced apoptosis in H9c2 cardiomyocytes and the underlying mechanisms. Sixteen novel CE anologues have been designed, synthesized and biological evaluation. Among the 16 CE anologues, as well as the positive control CE tested, compound 5d was the most effective in improving cardiomyocytes viability. Pretreatment with anologue 5d inhibited ROS generation, maintained the mitochondrial membrane potential and reduced apoptotic cardiomyocytes. Moreover, exposure to H2O2 significantly increased the levels of Bax, cleaved caspase-3, and cleaved PARP, and decreased the level of Bcl-2, resulting in cell apoptosis. Pretreatment with anologue 5d (0.02–0.5 μg/mL) dose-dependently upregulated antiapoptotic proteins and downregulated proapoptotic proteins mentioned above during H2O2-induced apoptosis. These results suggested that CE analogues provide protection to H9c2 cardiomyocytes against H2O2-induced oxidative stress and apoptosis, most likely via anti-apoptotic mechanism, and provided the basis for the further optimization of the CE analogues.
Published: 2017

24. Increased frequency of AMP-activated protein kinase-positive spinal motor neurons after sciatic nerve injury in a mouse model

Author: Gui-Jun Cao, Xiao Liang, Bao Qi, Qingwei Li, Chunyang Meng, Yu-Zhong Wang, and Guowu Chen
Subjects: 0301 basic medicine, Male, medicine.medical_specialty, Time Factors, Nerve Crush, Motor nerve, Action Potentials, Gene Expression, AMP-Activated Protein Kinases, 03 medical and health sciences, Mice, 0302 clinical medicine, AMP-activated protein kinase, Internal medicine, medicine, Animals, Motor Neurons, lcsh:R5-920, biology, business.industry, Skeletal muscle, AMPK, General Medicine, Nerve injury, Sciatic nerve injury, Motor neuron, medicine.disease, Sciatic Nerve, Axons, Nerve Regeneration, Mice, Inbred C57BL, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, nervous system, Spinal Cord, biology.protein, Sciatic nerve, medicine.symptom, Sciatic Neuropathy, lcsh:Medicine (General), business, 030217 neurology & neurosurgery
Abstract: The role of AMP-activated protein kinase (AMPK) in the regulation of energy metabolism and the control of skeletal muscle regeneration post injury has been described previously. It remains unknown whether this metabolic sensor plays a role in the mechanism of axonal regeneration post injury. In this study, we used a sciatic nerve crushed mouse model to detect the expression of AMPK in sciatic nerve and spinal motor neurons at 1 week, 2 weeks and 3 weeks after injury by immunofluorescence staining. Electrophysiological and histopathological studies were used to confirm the nerve injury and regeneration. Our results showed that frequency of AMPK-positive spinal motor neurons was significantly higher on day 7 after sciatic nerve crush (SNC) and peaked on day 14. No expression of AMPK was detected in axons of the sciatic nerve before and after the injury. Taken together, our study suggested a possible role of AMPK in the mechanism of motor nerve regeneration after injury. Keywords: Sciatic nerve injury, Motor neuron, AMP-Activated protein kinase
Published: 2017

25. Observations of Skin Tissue Regenerations in Biomaterial Scaffolds with Different Biodegradability

Author: Bao Qi Zuo, Liang Xie, Lun Bai, Xin Hong Wang, and Kui Hua Zhan
Subjects: Materials science, integumentary system, Regeneration (biology), General Engineering, Fibroin, Biomaterial, Biodegradation, medicine.disease, Polyvinyl alcohol, Neovascularization, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, medicine, medicine.symptom, Infiltration (medical), Biomedical engineering, Blood vessel
Abstract: Objective: To explore the effects of the biodegradability of biomaterial scaffolds on skin tissue regeneration and its neovascularization by the animal experiments. Methods: A piece of porous silk fibroin film (SF) of easy biodegradation and a piece of porous polyvinyl alcohol film (PVA) of hard biodegradation were implanted into a same skin wound of adult rat, and the differences in the regenerative blood vessel and tissue at 5, 10, 16, 41 days after surgery were observed by histological methods. Results: (1) at 10 days after surgery, the SF started to degrade, the closed areas without cell infiltration were opened up and the entire material was filled with the regenerative tissue, and there were still the closed areas in the PVA, (2) the new vascular network remodeling in the SF and PVA appeared at 10 days and 41 days after surgery, respectively, and (3) at 16 days after surgery, most of the SF material had degraded and substantially been replaced with the regenerative tissue, and the visible degradation of the PVA appeared at 41 days after surgery. Conclusion: The good biodegradability of the SF was helpful both to all vascularization of the material and to the regenerative blood vessels remodeling, and the regenerative tissue was closer to the normal dermal tissue than in the PVA.
Published: 2013

26. Correlation of Natural and Artificial UV Aging of B.mori Silk Fabric

Author: Bao Qi Zuo, Jin Fa Ming, Yao Xing Jiang, and Ji Shi
Subjects: Mechanical property, Materials science, fungi, Silk fabric, technology, industry, and agriculture, General Engineering, Ultraviolet protection, medicine.disease_cause, Breaking strength, SILK, medicine, Fourier transform infrared spectroscopy, Composite material, Spectroscopy, Ultraviolet
Abstract: Ultraviolet aging of B.mori silk fabric was carried out under natural and artificial weathering conditions. A series of properties evaluations such as whiteness, mechanical property, ultraviolet protection factor (UPF) and Fourier Transform Infrared (FTIR) Spectroscopy were applied to test on the structure and properties of B.mori silk fabric. The correlation between natural and artificial aging experiments was also investigated. The results showed that the whiteness index of B.mori silk surface declined with increasing aging time. The breaking strength decreased rapidly with the extension of aging time, and adopting the curve regression analysis method to analysis the correlation between two types of aging experiments. The accelerated factor obtained is about 12.33 between natural and artificial aging experiments. In addition, ultraviolet protection factor depicted the anti-ultraviolet performance enhanced. And FTIR spectra confirmed the intensity of characteristic peaks such as amide I and II, etc. decreasing in the aging process.
Published: 2012

27. Design, Synthesis and Evaluation of the Antidepressant and Anticonvulsant Activities of Triazole-Containing Benzo[d]oxazoles

Author: Ming-Xia Song, Bao-Qi Rao, Bin-Bin Cheng, Bentham Science Publisher Yi, Bentham Science Publisher Wu, Hong Zeng, You-gen Luo, and Xian-Qing Deng
Subjects: Male, Stereochemistry, medicine.medical_treatment, Drug Evaluation, Preclinical, Pharmacology, Motor Activity, 01 natural sciences, Open field, Epilepsy, Mice, Structure-Activity Relationship, Seizures, Medicine, Animals, Computer Simulation, GABA Modulators, ED50, gamma-Aminobutyric Acid, Benzoxazoles, Depressive Disorder, Electroshock, Molecular Structure, 010405 organic chemistry, business.industry, General Neuroscience, Neurotoxicity, medicine.disease, Tail suspension test, Antidepressive Agents, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, Anticonvulsant, Drug Design, Antidepressant, Anticonvulsants, business, Behavioural despair test
Abstract: Background Epilepsy and depression are two of the common diseases seriously threatening life and health of human. A shared neurobiological substrate led to the bidirectional relationship and high comorbid occurrence of the two disorders. Recently, an increasing number of patients with epilepsy (PWE) require some form of antidepressant medication. However, most of the available antidepressants are inadequate for PWE for some reasons. So, the search for novel and increasingly effective drugs with anticonvulsant and antidepressant activities is necessary. Methods A series of 2-substituted-6-(4H-1,2,4-triazol-4-yl)benzo[d]oxazoles (5a-p) were designed and synthesized. Their anticonvulsant activities were evaluated using maximal electroshock shock (MES) and subcutaneous pentylenetetrazole (scPTZ) seizure models in mice. Their antidepressant activities were screened with the forced swimming test (FST). Results All the compounds showed anti-MES activities in different degree, among which 5g and 5j were the most promising one with ED50 value of 31.7 and 12.7 mg/kg, respectively. What's more, 5g and 5j also exhibited nice anti-scPTZ activities and low neurotoxicity. Interestingly, these compounds also showed good antidepressant activities in FST. And the efficacy of 5g were also confirmed by a tail suspension test and a open field test. The pretreatment of thiosemicarbazide (an inhibitor of γ- aminobutyric acid synthesis enzyme) significantly increased the ED50 of 5g in MES and reversed the reductions in the immobility time of 5g in FST. Conclusion Triazole-containing benzo[d]oxazole is a good skeleton to develop compounds with both anticonvulsant and antidepressant activities. We have got the compound 5g, which display remarkable antidepressant and anticonvulsant activities, and the GABAergic system was involved in the action mechanism of 5g.
Published: 2016

28. Whole-body aerosol exposure of cadmium chloride (CdCl2) and tetrabromobisphenol A (TBBPA) induced hepatic changes in CD-1 male mice

Author: Yabing Hu, Huiying Zheng, Bishu Pan, Shuyun Liu, Hao Li, Yuanhong Chen, Xiaojuan Wu, Qiaoxiang Dong, Bao-qi Peng, Jinlie Long, Changjiang Huang, and Zhengyu Huang
Subjects: 0301 basic medicine, Male, medicine.medical_specialty, Environmental Engineering, Antioxidant, Health, Toxicology and Mutagenesis, medicine.medical_treatment, Polybrominated Biphenyls, chemistry.chemical_element, Apoptosis, Mice, Inbred Strains, 010501 environmental sciences, Cadmium chloride, medicine.disease_cause, 01 natural sciences, Antioxidants, Gene Expression Regulation, Enzymologic, 03 medical and health sciences, chemistry.chemical_compound, Mice, Cadmium Chloride, Liver Function Tests, Internal medicine, Occupational Exposure, medicine, Environmental Chemistry, Animals, Waste Management and Disposal, 0105 earth and related environmental sciences, Aerosols, Cadmium, Organ Size, Pollution, Oxidative Stress, Waste Disposal Facilities, 030104 developmental biology, Endocrinology, chemistry, Liver, Environmental chemistry, Toxicity, Phase II Detoxification, Tetrabromobisphenol A, Environmental Pollutants, Chemical and Drug Induced Liver Injury, Phase I Detoxification, Oxidative stress
Abstract: Cadmium (Cd) and tetrabromobisphenol A (TBBPA) are two prevalent contaminants in e-waste recycling facilities. However, the potential adversely health effect of co-exposure to these two types of pollutants in an occupational setting is unknown. In this study, we investigated co-exposure of these two pollutants on hepatic toxicity in CD-1 male mice through a whole-body aerosol inhalation route. Specifically, mice were exposed to solvent control (5% DMSO), Cd (8μg/m(3)), TBBPA (16μg/m(3)) and Cd/TBBPA mixture for 8h/day and 6days a week for 60 days. Hepatic changes include increased organ weight, focal necrosis, and elevated levels of liver enzymes in serum. These changes were most severe in mice exposed to TBBPA, followed by Cd/TBBPA mixture and Cd. These chemicals also led to suppressed antioxidant defensive mechanisms and increased oxidative stress. Further, these chemicals induced gene expression of apoptosis-related genes, activated genes encoding for phase I detoxification enzymes and inhibited genes encoding for phase II detoxification enzymes. These findings indicate that the hepatic damages induced by subchronic aerosol exposure of Cd and TBBPA may result from the oxidative damages caused by excessive ROS production when these chemicals were metabolized in the liver.
Published: 2016

29. In Vitro Evaluation of the Growth and Migration of Schwann Cells on Electrospun Silk Fibroin Nanofibers

Author: Bao Qi Zuo, Qing Lan, Ai Jun Hu, Huan Xiang Zhang, and Feng Zhang
Subjects: Materials science, Regeneration (biology), fungi, General Engineering, Nerve guidance conduit, Fibroin, Nerve injury, Neural tissue engineering, medicine.anatomical_structure, Tissue engineering, Nanofiber, Peripheral nervous system, medicine, Biophysics, medicine.symptom, Biomedical engineering
Abstract: Schwann cells (SCs) are primary structural and functional cells in peripheral nervous system and play a crucial role in peripheral nerve regeneration. Current challenge in peripheral nerve tissue engineering is to produce an implantable scaffold capable of bridging long nerve gaps and assist Scs in directing the growth of regenerating axons in nerve injury recovery. Electrospun silk fibroin nanofibers, fabricated for the cell culture in vitro, can provide such experiment support. Silk fibroin scaffolds (SFS) were fabricated with formic acid (FA), and the average fiber diameter was 305 ± 24 nm. The data from microscopic, immunohistochemical and scanning electron micrograph confirmed that the scaffold was beneficial to the adherence, proliferation and migration of SCs without exerting any significant cytotoxic effects on their phenotype. Thus, providing an experimental foundation accelerated the formation of bands of Bünger to enhance nerve regeneration. 305 nm SFS could be a candidate material for nerve tissue engineering.
Published: 2011

30. Neovascularization of Porous Silk Fibroin Films(PSFFs) as Dermis Substitutes Implanted in Rats

Author: Lun Bai, Zheng Yu Wu, Mingzhong Li, Bao Qi Zuo, Guo Ping Guan, and Zi Lin Sun
Subjects: Loose connective tissue, Materials science, Mechanical Engineering, Normal tissue, Fibroin, Connective tissue, Condensed Matter Physics, Neovascularization, medicine.anatomical_structure, Dermis, Mechanics of Materials, medicine, General Materials Science, medicine.symptom, Wound healing, Process (anatomy), Biomedical engineering
Abstract: Neovascularization in biomaterials plays a key role in wound healing. In this paper, porous silk fibroin films (PSFFs) whose structure was optimized, as dermis substitutes, were implanted in the back skin of rats for recovery of dermis loss. Results showed that a thin layer of loose connective tissue had formed at the interface between PSFFs and subcutaneous tissues, in which abundant blood vessels could be observed at 24 h after surgery. Whereafter the newly formed connective tissue thickened and the number of microvessels in the tissue increased. Furthermore, a few microvessels could be seen in PSFFs at day 5, most of which were capillaries. By day 10, the density of microvessels in PSFFs increased to a peak while the percentage of capillary decreased. At day 23, both the density of microvessels and the percentage of capillary was almost equal to those of normal tissues. In summary, wound healing with PSFFs as dermis substitutes is the neovascularization process of PSFFs. The process includes three major steps: (1) new formation and growth of loose connective tissue into the pores of PSFFs, (2) proliferation and migration of fibroblasts and endothelial cells, and (3) formation of functional microvessels and their networks.
Published: 2009

31. Current status and future prospects of acupuncture and traditional Chinese medicine in Canada

Author: Ben Bao Qi Cao
Subjects: medicine.medical_specialty, Medical education, Canada, Traditional medicine, Education, Medical, business.industry, media_common.quotation_subject, Supplementary benefit, Immigration, Alternative medicine, Acupuncture Therapy, Legislation as Topic, Legislation, General Medicine, Traditional Chinese medicine, Complementary and alternative medicine, Blueprint, Acupuncture, Medicine, Humans, Pharmacology (medical), Medicine, Chinese Traditional, business, Ethical code, media_common
Abstract: Traditional Chinese medicine (TCM) and acupuncture has been gradually accepted by the mainstream society since the 1970s and has been rapidly expanding, accompanying the tide of Chinese immigration since the 1980s. Acupuncture legislation has been approved by the governments of 5 provinces in Canada between 1988 and 2014. After the acceptance of self-regulation, 5 corresponding colleges were established, one after another. The code of ethics and professional practice standard were developed by the Colleges. British Columbia (BC) became the first Canadian province to offer acupuncture treatments as a supplementary benefit for its Medical Services Plan premium assistance recipients beginning in 2008. The 5 colleges united to form the Canadian Alliance of Regulatory Bodies of Traditional Chinese Medicine Practitioners and Acupuncturists (CARBTCMPA) in the same year. The Entry-level Occupational Competencies for the Practice of TCM in Canada were developed by CARB-TCMPA in 2010. The Pan-Canadian registration examinations for acupuncturists, herbalists and TCM practitioners have been administered and scheduled since 2013. The Entry-level Occupational Competencies for Doctors of TCM were developed by College of Traditional Chinese Medicine Practitioners and Acupuncturists of British Columbia (CTCMA-BC) in 2014. It sketches the blueprint for future development of the TCM profession in Canada.
Published: 2014

32. Successful treatment of hailey-hailey disease with aminolevulinic Acid photodynamic therapy

Author: Fu Ren Zhang, Xiao Xiao Yan, Chang Liang Wang, Hong Qing Tian, Wei Zhi Wu, and Bao Qi Yang
Subjects: medicine.medical_specialty, Erythema, Groin, business.industry, medicine.drug_class, medicine.medical_treatment, Antibiotics, Photodynamic therapy, Dermatology, medicine.disease, Surgery, Light source, medicine.anatomical_structure, Hailey–Hailey disease, Vitamin D and neurology, Medicine, medicine.symptom, Family history, business, Letter to the Editor
Abstract: Dear Editor: A 40-year-old man presented with a 3-year history of Hailey-Hailey disease (HHD). Painful axillary and inguinal vesicles, erosions, and plaques existed (Fig. 1A, B). There was no family history of HHD. The diagnosis was confirmed by histopathologic examination. He had been treated with topical steroids, vitamin D analogs, and oral antibiotics, and short-term remission had been achieved. As soon as he discontinued the treatment, the lesions relapsed. The patient requested other treatments; thus, aminolevulinic acid photodynamic therapy (ALA-PDT) was offered. Fig. 1 (A, B) Erythema and erosions in the axillary area and inguinal area. (C, D) The clinical situation after three treatments of aminolevulinic acid photodynamic therapy (ALA-PDT) therapy every 2 wk. (E, F) The clinical situation after five treatments of ... After informed consent was obtained, we initiated a course of ALA-PDT, which consisted of five treatment sessions over 2-wk intervals. The patient did not use any topical or systemic therapy during the ALA-PDT treatment. At each visit, 10% ALA cream was applied to the axillae and groin for 3 h, followed by irradiation with red light (623~643 nm). A non-coherent light-emitting diode light source was used with a fluence rate of 20~40 mW/cm2 and a light dose of 37 J/cm2 for 20 min. The patient had mild burning in the lesions during light irradiation, but it was tolerable and did not require local anesthetics or sedatives; the discomfort generally resolved after 2 days. No other side effects were noted. After three sessions of ALA-PDT therapy, the patient was clinically improved with flattening of the keratotic papules and a reduction in erythema and erosions (Fig. 1C, D). There was a gradual improvement in the signs and symptoms during the treatment period. A marked decrease in erythema and erosions in the axillary and inguinal regions was achieved after five treatments (Fig. 1E, F). The patient had no relapse in the treated areas during a 12-month follow-up period. There have been five reported cases of HHD effectively treated with PDT1,2. Indeed, these reports served as the impetus for treating our patient with ALA-PDT. However, a different outcome was reported as previous studies. Because HHD is characterized by vesicular lesions, painful erosions and plaques occur at the sites of friction; therefore, treatment should be different from that used for condylomata acuminata or thick skin tumors. In an effort to prevent patient discomfort and improve efficacy, we selected a treatment for HHD with a lower concentration of ALA intensity and more than one PDT cycle compared with previous studies1,2. Our results were good-the patient had mild discomfort during the light exposure and for the first 2 days post-treatment, otherwise the treatment was well tolerated.
Published: 2014

33. Interracial comparative study of prostate cancer in the United States, China, and Japan

Author: Lian-Sheng Zhao, Bao-Qi Chen, Haiyen E. Zhau, and Munekado Kojima
Subjects: PCA3, Oncology, medicine.medical_specialty, biology, business.industry, Urology, Chromogranin A, Cell Biology, Disease, medicine.disease, Biochemistry, Androgen receptor, Prostate cancer, medicine.anatomical_structure, Endocrinology, Patient age, Prostate, Internal medicine, medicine, biology.protein, Immunohistochemistry, Stage (cooking), China, business, Molecular Biology
Abstract: The interracial differences of prostate cancer progression have long been documented; however, underlying molecular and cellular mechanisms remain obscure. This study focuses on the histopathologic, immunohistochemical, biochemical, and molecular characterization of prostate cancer tissues unselectively obtained from US, Chinese, and Japanese men. Histopathologic analyses indicate that 74.5% of the prostate cancers in Chinese patients were poorly differentiated, compared with 28.6 and 32.8% of the prostate cancers in US and Japanese men, respectively. These differences cannot be attributed to patient age, clinical stage of disease, or methods of tissue sampling. Furthermore, the high proportion of poorly differentiated prostate cancer tissues in the Chinese group was not related to the patients' access to medical service or their geographic origins within China. We found significantly higher levels of tumor angiogenesis (2- to 4-fold), serotonin (2- to 20-fold), and bombesin (7- to 16-fold), but not chromogranin A, in tissue specimens obtained from Chinese prostate cancer patients compared with those from US and Japanese patients. We also found marked differences in p53 protein accumulation among various ethnic groups. The p53 protein was frequently detected in prostate cancer tissue specimens from Chinese (90.2%), but less frequently in US black (3.7%), US white (17.4%), and Japanese (7.1%) men. Further analysis of 31 prostate cancer tissues from Chinese men indicated that mutational changes in the p53 gene occurred between exons 5 and 8. J. Cell. Biochem. Suppls. 28/29:182–186. © 1998 Wiley-Liss, Inc.
Published: 1997

34. Persistent whole-chromosome aneuploidy is generally associated with nascent allohexaploid wheat

Author: Huakun Zhang, Bao Liu, Ning Li, Fangpu Han, Xiaowan Gou, Hao Zhou, Sachin Rustgi, Bao Qi, Chunming Xu, Bo Zhu, Jiming Jiang, Yao Bian, and Diter von Wettstein
Subjects: Aneuploidy, Biology, Genome, Chromosomes, Plant, Polyploidy, Homologous chromosome, medicine, Common wheat, Crosses, Genetic, Triticum, Genetics, Plant evolution, Tissue Survival, Multidisciplinary, food and beverages, Reproducibility of Results, Karyotype, Sequence Analysis, DNA, Biological Sciences, medicine.disease, Karyotyping, Seeds, Pyrosequencing, Pollen, Ploidy
Abstract: Allopolyploidization has been a driving force in plant evolution. Formation of common wheat ( Triticum aestivum L.) represents a classic example of successful speciation via allopolyploidy. Nevertheless, the immediate chromosomal consequences of allopolyploidization in wheat remain largely unexplored. We report here an in-depth investigation on transgenerational chromosomal variation in resynthesized allohexaploid wheats that are identical in genome constitution to common wheat. We deployed sequential FISH, genomic in situ hybridization (GISH), and homeolog-specific pyrosequencing, which enabled unequivocal identification of each of the 21 homologous chromosome pairs in each of >1,000 individual plants from 16 independent lines. We report that whole-chromosome aneuploidy occurred ubiquitously in early generations (from selfed generation S 1 to >S 20 ) of wheat allohexaploidy although at highly variable frequencies (20–100%). In contrast, other types of gross structural variations were scant. Aneuploidy included an unexpected hidden type, which had a euploid chromosome number of 2 n = 42 but with simultaneous loss and gain of nonhomeologous chromosomes. Of the three constituent subgenomes, B showed the most lability for aneuploidy, followed by A, but the recently added D subgenome was largely stable in most of the studied lines. Chromosome loss and gain were also unequal across the 21 homologous chromosome pairs. Pedigree analysis showed no evidence for progressive karyotype stabilization even with multigenerational selection for euploidy. Profiling of two traits directly related to reproductive fitness showed that although pollen viability was generally reduced by aneuploidy, the adverse effect of aneuploidy on seed-set is dependent on both aneuploidy type and synthetic line.
Published: 2013

35. Transfectedneu oncogene induces human prostate cancer metastasis

Author: Haiyen E. Zhau, Shi Ming Chang, Jianxin Zhou, Robert A. Sikes, Bao Qi Chen, Leland W.K. Chung, and W. Fraser Symmans
Subjects: Pathology, medicine.medical_specialty, biology, Oncogene, Urology, Cell, Clone (cell biology), Vimentin, Transfection, medicine.disease, Metastasis, medicine.anatomical_structure, Oncology, Cell culture, Cell Clone, medicine, biology.protein, Cancer research
Abstract: c-erb B2/neu has been demonstrated to be a transforming oncogene in both rodent and human prostatic epithelial cells. To understand the potential role of neu in human prostatic cancer progression, we used a gene transfer procedure to determine whether neu amplification/overexpression leads to increased tumor growth and metastasis. We chose an androgen-independent human prostatic epithelial cell line, PC-3, as the target for gene transfer. PC-3 cells were cotransfected with pSVneu-T (a point-mutated rat neu oncogene construct) and pSV2neo, and single-cell cloned. Fifty cell clones were isolated and characterized, of which two neu-transfected clones (N17 and N35) and a neo control clone (C32) were studied extensively with respect to neu gene integration, levels of neu mRNA and protein expression, anchorage-independent growth, and tumorigenic and metastatic potential. Results showed that: 1) Clone N35 contained 70 copies of the neu oncogene and a high level of neu mRNA transcripts. It acquired increased anchorage-independent growth potential in vitro and increased tumorigenicity in vivo. 2) Clone N17 contained 10 copies of the neu oncogene and a low level of neu mRNA transcripts. It did not acquire additional capability for anchorage-independent growth and tumorigenic potential as compared to the controls. 3) Despite an increased level of neu mRNA transcripts present in clone N35, there was no corresponding increase of the steady-state levels of neu protein in this particular clone. 4) When administered subcutaneously, none of the cell clones tested, including the control neomycin-resistant clone, acquired metastatic potential. However, clone N35 exhibited marked metastatic potential when administered orthotopically; this cell clone was found to disseminate widely to the lymph nodes, kidney, skeletal muscle, lung, liver, and bone. 5) When neu-transfected cell subclones from N35-induced primary and metastatic lymph node, kidney, and bone tumors were analyzed for cytoskeletal, extracellular matrix, and cell adhesion protein expression, the bone metastatic subclone exhibited increased levels of vimentin and collagen IV and decreased levels of cytokeratin and ICAM-1. These results, taken together, suggest that neu transfection induces secondary changes, which, rather than neu protein per se, are responsible for the acquisition of tumorigenic and metastatic potential of prostate cancer cells when an appropriate host microenvironment is present. © 1996 Wiley-Liss, Inc.
Published: 1996

36. The Development of Occupational Exposure Limits for Chemical Substances in China

Author: Bao-Qi Gang, Xue-Qi Gu, and You-Xin Liang
Subjects: China, education.field_of_study, business.industry, Population, Administrative system, Air Pollutants, Occupational, General Medicine, Toxicology, Management, Risk analysis (engineering), Occupational Exposure, Carcinogens, Animals, Humans, Medicine, Maximum Allowable Concentration, Occupational exposure, education, business, Selection (genetic algorithm)
Abstract: This paper presents a comprehensive review of the occupational exposure limits (OELs) of chemical substances in China. It provides historical background on the development of OELs in this country, with a complete list of traditionally adopted and newly developed OELs for chemicals in workplaces. The philosophical thoughts, the administrative system, the scientific protocols for setting and amending health standards, with emphasis on making health a basic criterion for setting health standards, strengthening epidemiological studies of the human population, integrating epidemiological and toxicological studies, considering technological and economical feasibilities, and making full use of literature information sources are discussed. Further perspectives with respect to practical issues of maximum allowable concentration and time-weighted average, selection of safety factors, and establishment of biological exposure limits are also considered, with the authors′ contributions to a discussion on these topics.
Published: 1995

37. Comparative studies of prostate cancers among United States, Chinese, and Japanese patients: characterization of histopathology, tumor angiogenesis, neuroendocrine factors, and p53 protein accumulations

Author: Lian Sheng Zhao, Rodney Davis, Judd W. Moul, Bao Qi Chen, Haiyen E. Zhau, Ninjia Zheng, M. Craig Hall, Patricia Troncoso, Leland W.K. Chung, Chinghai Kao, Ming Fu Ye, W. Fraser Symmans, Lian Sheng Xiao, Munekado Kojima, and J. L. Palmer
Subjects: PCA3, medicine.medical_specialty, Pathology, biology, business.industry, Angiogenesis, Urology, Chromogranin A, medicine.disease, Prostate cancer, medicine.anatomical_structure, Oncology, Prostate, biology.protein, Medicine, Immunohistochemistry, Histopathology, Stage (cooking), business
Abstract: Although interracial differences of prostate cancer progression are well recognized, their underlying molecular and cellular mechanisms remain obscure. We compared the histopathologic, immunohistochemical, and molecular characteristics of unselected prostate cancer tissues obtained from U.S., Chinese, and Japanese men. Histopathologic analyses indicated that 74.4% of the prostate cancers in Chinese men were poorly differentiated, compared with 28.6% and 32.8% of the prostate cancers in U.S. and Japanese men, respectively. These differences cannot be attributed to patient age, clinical stage of disease, or methods of tissue sampling. The high proportion of poorly differentiated prostate cancer tissues in the Chinese group was not related to the patients' access to medical service or to geographic background within China. Significantly higher levels of tumor angiogenesis (2- to 4-fold), serotonin (2- to 20-fold), and bombesin (7- to 16-fold), but not chromogranin A, were found in the tissue specimens obtained from Chinese prostate cancer patients compared with those from U.S. and Japanese patients. We also observed marked interracial differences in p53 protein accumulation. The protein was present in 90.2% of Chinese specimens; 17.4% of specimens from U.S. whites; 7.1% of specimens from Japanese men; and 3.7% of specimens from U.S. blacks. Results from multivariate logistic regression analysis demonstrated that p53 protein accumulation, angiogenesis, and serotonin expression in the normal stroma area correlate independently with Chinese versus non-Chinese patient populations.
Published: 2011

38. In vitro and in vivo inhibitory effect evaluation of cyclooxygenase-2 inhibitors, antisense cyclooxygenase-2 cDNA, and their combination on the growth of human bladder cancer cells

Author: Heliang Liu, Ling Li, Kaichun Wu, Jun Qin, Jianlin Yuan, Weijun Qin, Rong-liang Qin, Bao-Qi Chen, and He Wang
Subjects: Male, medicine.medical_specialty, DNA, Complementary, Indomethacin, Mice, Nude, Apoptosis, DNA, Antisense, Gene Expression Regulation, Enzymologic, chemistry.chemical_compound, Mice, Internal medicine, Cell Line, Tumor, Antineoplastic Combined Chemotherapy Protocols, medicine, Animals, Humans, Cyclooxygenase Inhibitors, Cell Proliferation, Pharmacology, Mice, Inbred BALB C, Sulfonamides, Bladder cancer, biology, Cyclooxygenase 2 Inhibitors, Cell growth, business.industry, Cancer, General Medicine, medicine.disease, Xenograft Model Antitumor Assays, Endocrinology, chemistry, Urinary Bladder Neoplasms, Celecoxib, Cyclooxygenase 2, Cancer cell, Cancer research, biology.protein, COX-2 inhibitor, Pyrazoles, Cyclooxygenase, Growth inhibition, business, medicine.drug
Abstract: Overexpression of cyclooxygenase (COX)-2 is associated with the progression of various malignancies, but the contribution of COX-2 expression, bioactivity or their cooperation to bladder cancer growth calls for further clarification. In this study, we investigated the inhibitory effect of COX-2 inhibitors, antisense COX-2 nucleotide, and their combination on the growth of bladder cancer cells (5637, 5637-P and 5637-AS). Suppression of either COX-2 expression or activity caused reduced cell proliferation, enhanced cell numbers in G(1) phase, and increased apoptosis; the joint suppression of COX-2 expression and bioactivity enhanced the degree of cell growth inhibition. COX-2 antisense-expressing 5637-AS tumors showed a 41.42+/-3.08% growth inhibition as compared with 5637 controls. Oral administration of indomethacin (3mg/kg) or celecoxib (15 mg/kg) caused tumor growth inhibition by 31.5+/-14.87% or 83.17+/-1.17%, respectively. When COX-2 antisense cDNA and COX-2 inhibitor celecoxib were combined, the tumor growth inhibition rate was further increased up to 88.78+/-3.10%. These results provide evidence that celecoxib has potential therapeutic effect on bladder cancer, and the joint use of COX-2 antisense cDNA with celecoxib may improve their individual therapeutic effect, especially significantly increase the growth inhibitory effect of COX-2 antisense cDNA.
Published: 2008

39. Androgen-repressed phenotype in human prostate cancer

Author: Chinghai Kao, Hongquan Zhang, Sen Pathak, Shi-Ming Chang, Leland W.K. Chung, Qing-Xiang Amy Sang, Yunling Wang, Bao-Qi Chen, and Haiyen E. Zhau
Subjects: Genetic Markers, Male, medicine.medical_specialty, medicine.drug_class, medicine.medical_treatment, Transplantation, Heterologous, Mice, Nude, Cell Line, Prostate cancer, Mice, Internal medicine, LNCaP, medicine, Tumor Cells, Cultured, Animals, Humans, Epidermal growth factor receptor, Neoplasm Metastasis, Aged, Aged, 80 and over, Multidisciplinary, biology, Estradiol, Growth factor, Chromogranin A, Cancer, Ascites, Prostatic Neoplasms, Dihydrotestosterone, Estrogens, Biological Sciences, Androgen, medicine.disease, Recombinant Proteins, Androgen receptor, Endocrinology, Phenotype, Receptors, Androgen, Karyotyping, Lymphatic Metastasis, Cancer research, biology.protein, Androgens, Mutagenesis, Site-Directed, Cell Division
Abstract: An androgen-repressed human prostate cancer cell line, ARCaP, was established and characterized. This cell line was derived from the ascites fluid of a patient with advanced metastatic disease. In contrast to the behavior of androgen-dependent LNCaP and its androgen-independent C4-2 subline, androgen and estrogen suppress the growth of ARCaP cells in a dose-dependent manner in vivo and in vitro . ARCaP is tumorigenic and highly metastatic. It metastasizes to the lymph node, lung, pancreas, liver, kidney, and bone, and forms ascites fluid in athymic hosts. ARCaP cells express low levels of androgen receptor mRNA and prostate-specific antigen mRNA and protein. Immunohistochemical staining shows that ARCaP cells stain intensely for epidermal growth factor receptor, c-erb B2/ neu , and c-erb B3. Staining is negative for chromogranin A and positive for bombesin, serotonin, neuron-specific enolase, and the c-met protooncogene (a hepatic growth factor/scatter factor receptor). ARCaP cells also secrete high levels of gelatinase A and B and some stromelysin, which suggests that this cell line may contain markers representing invasive adenocarcinoma with selective neuronendocrine phenotypes. Along with its repression of growth, androgen is also found to repress the expression of prostate-specific antigen in ARCaP cells as detected by a prostate-specific antigen promoter–β-galactosidase reporter assay. Our results suggest that the androgen-repressed state may be central to prostate cancer progression and that advanced prostate cancer can progress from an androgen-independent to an androgen-repressed state.
Published: 1996

40. Tissue distribution of sialic acid-linked influenza virus receptors in beagle dogs

Author: Yan Fen Cheng, Yu Fu An, Wen Bao Qi, Guihong Zhang, Shou Jun Li, Heng Wang, Xin Tao Wu, and Zhang Yong Ning
Subjects: Male, dogs, Receptors, Cell Surface, medicine.disease_cause, Beagle, Virus, Pathogenesis, chemistry.chemical_compound, Agglutinin, Orthomyxoviridae Infections, Sambucus nigra, Influenza A virus, medicine, Animals, Maackia, Dog Diseases, Receptor, Influenza A Virus, H5N1 Subtype, General Veterinary, biology, influenza virus receptors, Lectin, Virology, N-Acetylneuraminic Acid, Sialic acid, chemistry, sialic acid, Organ Specificity, biology.protein, lectin, Receptors, Virus, Original Article, Female, Plant Lectins
Abstract: Reports of influenza A virus infections in dogs has received considerable attention from veterinarians, virologists, and epidemiologists. Interaction between influenza viral hemagglutinin and cell oligosaccharides containing sialic acid residues results in infection. Sialic acids have an α-2,3-linkage to the penultimate galactose in the avian influenza virus receptor and an α-2,6-linkage in the human receptor. To date, there are no detailed data on the tissue distribution or histological features of either type of sialic acid-linked influenza virus receptors in beagle dogs, which are common laboratory animals and pets. We conducted the current study to visualize the in situ tissue distribution of both sialic acid-linked influenza virus receptors in various organs of beagle dogs using Maackia amurensis lectin II and Sambucus nigra agglutinin. Both α-2,3- and α-2,6-sialic acid-linked receptors were detected in the endothelial cells of the respiratory tract and other organs. Endothelial cells of most gastrointestinal organs were negative for α-2,3-sialic acid-linked receptors in the dogs. Our results suggested that these canine organs may be affected by influenza virus infection. The findings from our study will also help evaluate the occurrence and development of influenza virus infections in dogs.
Published: 2012

41. Genetic characterization of H1N2 influenza a virus isolated from sick pigs in Southern China in 2010

Author: Shang Song Guan, Liang Quan Zhang, Nan Cao, Ming Liao, Weili Kong, Guihong Zhang, Wen Bao Qi, Liang Zong Huang, Haitao Qi, Heng Wang, and Pei Rong Jiao
Subjects: China, Genotype, Swine, viruses, Molecular Sequence Data, Reassortment, Population, Short Report, Genome, Viral, Biology, medicine.disease_cause, Virus, lcsh:Infectious and parasitic diseases, Evolution, Molecular, Viral Proteins, Orthomyxoviridae Infections, Influenza A Virus, H1N2 Subtype, Nasopharynx, Virology, Reassortant Viruses, medicine, Influenza A virus, Animals, Cluster Analysis, lcsh:RC109-216, education, Phylogeny, Swine Diseases, education.field_of_study, Foot-and-mouth disease, virus diseases, Sequence Analysis, DNA, medicine.disease, Influenza A virus subtype H5N1, Infectious Diseases, RNA, Viral
Abstract: In China H3N2 and H1N1 swine influenza viruses have been circulating for many years. In January 2010, before swine were infected with foot and mouth disease in Guangdong, some pigs have shown flu-like symptoms: cough, sneeze, runny nose and fever. We collected the nasopharyngeal swab of all sick pigs as much as possible. One subtype H1N2 influenza viruses were isolated from the pig population. The complete genome of one isolate, designated A/swine/Guangdong/1/2010(H1N2), was sequenced and compared with sequences available in GenBank. The nucleotide sequences of all eight viral RNA segments were determined, and then phylogenetic analysis was performed using the neighbor-joining method. HA, NP, M and NS were shown to be closely to swine origin. PB2 and PA were close to avian origin, but NA and PB1were close to human origin. It is a result of a multiple reassortment event. In conclusion, our finding provides further evidence about the interspecies transmission of avian influenza viruses to pigs and emphasizes the importance of reinforcing swine influenza virus (SIV) surveillance, especially before the emergence of highly pathogenic FMDs in pigs in Guangdong.
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42. Global patient outcomes after elective surgery: prospective cohort study in 27 low-, middle- and high-income countries

Author: Ahmad, T, Bouwman, RA, Grigoras, I, Aldecoa, C, Hofer, C, Hoeft, A, Holt, P, Fleisher, LA, Buhre, W, Pearse, RM, Ferguson, M, MacMahon, M, Shulman, M, Cherian, R, Currow, H, Kanathiban, K, Gillespie, D, Pathmanathan, E, Phillips, K, Reynolds, J, Rowley, J, Douglas, J, Kerridge, R, Garg, S, Bennett, M, Jain, M, Alcock, D, Terblanche, N, Cotter, R, Leslie, K, Stewart, M, Zingerle, N, Clyde, A, Hambidge, O, Rehak, A, Cotterell, S, Huynh, WBQ, McCulloch, T, Ben-Menachem, E, Egan, T, Cope, J, Halliwell, R, Fellinger, P, Haisjackl, M, Haselberger, S, Holaubek, C, Lichtenegger, P, Scherz, F, Schmid, W, Hoffer, F, Cakova, V, Eichwalder, A, Fischbach, N, Klug, R, Schneider, E, Vesely, M, Wickenhauser, R, Grubmueller, KG, Leitgeb, M, Lang, F, Toro, N, Bauer, M, Laengle, F, Haberl, C, Mayrhofer, T, Trybus, C, Buerkle, C, Forstner, K, Germann, R, Rinoesl, H, Schindler, E, Trampitsch, E, Bogner, G, Dankl, D, Duenser, M, Fritsch, G, Gradwohl-Matis, I, Hartmann, A, Hoelzenbein, T, Jaeger, T, Landauer, F, Lindl, G, Lux, M, Steindl, J, Stundner, O, Szabo, C, Bidgoli, J, Verdoodt, H, Forget, P, Kahn, D, Lois, F, Momeni, M, Pregardien, C, Pospiech, A, Steyaert, A, Veevaete, L, De Kegel, D, De Jongh, K, Foubert, L, Smitz, C, Vercauteren, M, Poelaert, J, Van Mossevelde, V, Abeloos, J, Bouchez, S, Coppens, M, De Baerdemaeker, L, Deblaere, I, De Bruyne, A, De Hert, S, Fonck, K, Heyse, B, Jacobs, T, Lapage, K, Moerman, A, Neckebroek, M, Parashchanka, A, Roels, N, Van Den Eynde, N, Vandenheuvel, M, Van Limmen, J, Vanluchene, A, Vanpeteghem, C, Wouters, P, Wyffels, P, Huygens, C, Vandenbempt, P, Van de Velde, M, Dylst, D, Janssen, B, Schreurs, E, Aleixo, FB, Candido, K, Batista, HD, Guimaraes, M, Guizeline, J, Hoffmann, J, Lobo, S, Marques Lobo, FR, Nascimento, V, Nishiyama, K, Pazetto, L, Souza, D, Rodrigues, RS, Vilela dos Santos, AM, Jardim, J, Sa Malbouisson, LM, Silva, J, Do Nascimento Junior, P, Baio, TH, Pereira de Castro, GI, Watanabe Oliveira, HR, Amendola, CP, Cardoso, G, Ortega, D, Brotto, AF, De Oliveira, MC, Rea-Neto, A, Dias, F, Travi, ME, Zerman, L, Azambuja, P, Knibel, MF, Martins, A, Almeida, W, Neder Neto, C, Tardelli, MA, Caser, E, Machado, M, Aguzzoli, C, Baldisserotto, S, Tabajara, FB, Bettega, F, Rodrigues Junior, LHC, De Gasperi, J, Faina, L, Nolasco, MF, Da Costa Fischer, BF, De Campos Ferreira, MF, Hartmann, C, Kliemann, M, Ribeiro, GLH, Fraga, JM, Netto, TM, Pozza, LV, Wendling, PR, Azevedo, C, Garcia, J, Lopes, M, Maia, B, Maselli, P, Melo, R, Mendes, W, Neves, M, Ney, J, Piras, C, Applewhaite, C, Carr, A, Chow, L, Duttchen, K, Foglia, J, Greene, M, Hinther, A, Houston, K, McCormick, TJ, Mikhayel, J, Montasser, S, Ragan, A, Suen, A, Woolsey, A, Yu, HC, Funk, D, Kowalski, S, Legaspi, R, McDonald, H, Siddiqui, F, Pridham, J, Rowe, B, Sampson, S, Thiessen, B, Zbitnew, G, Bernard, A, George, R, Jones, P, Moor, R, Siddiqui, N, Wolfer, A, Tran, D, Winch, D, Dobson, G, McCormick, T, Montasser, O, Hall, R, Baghirzada, L, Curley, G, Dai, SY, Hare, G, Lee, E, Shastri, U, Tsui, A, Yagnik, A, Alvares, D, Choi, S, Dwyer, H, Flores, K, McCartney, C, Somascanthan, P, Beattie, S, Carroll, J, Pazmino-Canizares, J, Wijeysundera, D, Ami, N, Chan, V, Perlas, A, Argue, R, Huang, Y, Lavis, K, Mayson, K, Cao, Y, Gao, H, Hu, T, Lv, J, Yang, J, Yang, Y, Zhong, Y, Zhou, J, Zou, X, He, M, Li, X, Luo, D, Wang, H, Yu, T, Chen, L, Wang, L, Cai, Y, Cao, Z, Li, Y, Lian, J, Sun, H, Wang, S, Wang, Z, Wang, K, Zhu, Y, Du, X, Fan, H, Fu, Y, Huang, L, Hwan, H, Luo, H, Qu, P-S, Tao, F, Wang, G, Zhang, Y, Zhang, X, Chen, C, Wang, W, Liu, Z, Fan, L, Tang, J, Chen, Y, Han, Y, Huang, C, Liang, G, Shen, J, Wang, J, Yang, Q, Zhen, J, Zhou, H, Chen, J, Chen, Z, Meng, B, Ye, H, Bi, Y, Cao, J, Guo, F, Lin, H, Liu, Y, Lv, M, Shi, P, Song, X, Sun, C, Sun, Y, Wang, Y, Zhang, M, Chen, R, Hou, J, Leng, Y, Meng, Q-T, Qian, L, Shen, Z-Y, Xia, Z-Y, Xue, R, Zhao, B, Zhou, X-J, Chen, Q, Guo, H, Guo, Y, Qi, Y, Wei, J, Zhang, W, Zheng, L, Bao, Q, Fei, Y, Hu, N, Hu, X, 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Carolyn, Panckhurst, Jonathan, Sara, Rachel, Stapelberg, Francoi, Campbell, Doug, Cherrett, Veronica, Ganter, Donna Louise, Mccann, Lloyd, Gilmour, Fiona, Lumsden, Rachelle, Moores, Mark, Olliff, Sue, Sardareva, Elitza, Tai, Joyce, Wikner, Matthew, Wong, Christopher, Chaddock, Mark, Czepanski, Carolyn, Mckendry, Patrick, Polakovic, Daniel, Polakovich, Daniel, Robert, Axe, Belda, Margarita Tormo, Norton, Tracy, Alherz, Fadhel, Barneto, Lisa, Ramirez, Alberto, Sayeed, Ahmed, Smith, Nicola, Bennett, Cambell, Mcquoid, Shane, Jansen, Tracy-Lee, Nico, Zin, Scott, John, Freschini, David, Freschini, Angela, Hopkins, Brian, Manson, Lara, Stoltz, Deon, Bates, Alexander, Davis, Simon, Freeman, Victoria, Mcgaughran, Lynette, Williams, Maya, Sharma, Swarna Baskar, Burrows, Tom, Byrne, Kelly, English, Duane, Johnson, Robert, Manikkam, Brendon, Naidoo, Shaun, Rumball, Margot, Whittle, Nicola, Franks, Romilla, Gibson-Lapsley, Hannah, Salter, Ryan, Walsh, Dean, Cooper, Richard, Perry, Katherine, Obobolo, Amo, Sule, Umar Musa, Ahmad, Abdurrahman, Atiku, Mamuda, Mohammed, Alhassan Datti, Sarki, Adamu Muhammad, Adekola, Oyebola, Akanmu, Olanrewaju, Durodola, Adekunle, Olukoju, Olusegun, Raji, Victor, Olajumoke, Tokunbo, Oyebamiji, Emmanuel, Adenekan, Anthony, Adetoye, Adedapo, Faponle, Folayemi, Olateju, Simeon, Owojuyigbe, Afolabi, Talabi, Ademola, Adenike, Odewabi, Adewale, Badru, Collins, Nwokoro, Ezekiel, Emmanuel, Fatungase, Oluwabunmi Motunrayo, Grace, Anuforo, Sola, Sotannde, Stella, Ogunmuyiwa, Ademola, Adeyinka, Adeolu, Augustine A., Adigun, Tinuola, Akinwale, Mukaila, Fasina, Oluyemi, Gbolahan, Olalere, Idowu, Olusola, Olonisakin, Rotimi Peter, Osinaike, Babatunde Babasola, Asudo, Felicia, Mshelia, Danladi, Abdur-Rahman, Lukman, Agodirin, Olayide, Bello, Jibril, Bolaji, Benjamin, Oyedepo, Olanrewaju Olubukola, Ezike, Humphrey, Iloabachie, Ikechukwu, Okonkwo, Ikemefuna, Onuora, Elia, Onyeka, Tonia, Ugwu, Innocent, Umeh, Friday, Alagbe-Briggs, Olubusola, Dodiyi-Manuel, Amabra, Echem, Richard, Obasuyi, Bright, Onajin-Obembe, Bisola, Bandeira, Maria Expedito, Martins, Alda, Tomã©, Miguel, Costa, Ana Cristina Miranda Martin, Krystopchuk, Andriy, Branco, Teresa, Esteves, Simao, Melo, Marco AntÃ³nio, Monte, Jãºlia, Rua, Fernando, Martins, Isabel, Pinho-Oliveira, VÃtor Miguel, Rodrigues, Carla Maria, Cabral, Raquel, Marques, Sofia, Rãªgo, Sara, Jesus, Joana Sofia Teixeira, Marques, Maria ConceiÃ§Ã£o, Romao, Cristina, Dias, Sandra, Santos, Ana Margarida, Alves, Maria Joao, Salta, Cristina, Cruz, Salome, Duarte, Cã©lia, Paiva, AntÃ³nio Armando Furtado, Do Nascimento Cabral, Tiago, Faria Maia, Dionisio, Correia Da Silva, Rui Freitas MendonÃ§a, Langner, Anuschka, Resendes, HernÃ¢ni Oliveira, Da ConceiÃ§Ã£o Soares, Maria, Abrunhosa, Alexandra, Faria, Filomena, Miranda, Lina, Pereira, Helena, Serra, Sofia, Ionescu, Daniela, Margarit, Simona, Mitre, Calin, Vasian, Horatiu, Manga, Gratiela, Stefan, Andreea, Tomescu, Dana, Filipescu, Daniela, Paunescu, Marilena-Alina, Stefan, Mihai, Stoica, Radu, Gavril, Laura, Pç trç å canu, Emilia, Ristescu, Irina, Rusu, Daniel, Diaconescu, Ciresica, Iosep, Gabriel Florin, Pulbere, Dorin, Ursu, Irina, Balanescu, Andreea, Grintescu, Ioana, Mirea, Liliana, Rentea, Irina, Vartic, Mihaela, Lupu, Mary-Nicoleta, Stanescu, Dorin, Streanga, Lavinea, Antal, Oana, Hagau, Natalia, Patras, Dumitru, Petrisor, Cristina, Tosa, Flaviu, Tranca, Sebastian, Copotoiu, Sanda Maria, Ungureanu, Liviu Lucian, Harsan, Cristian Remu, Papurica, Mariu, Cernea, Daniela Denisa, Dragoescu, Nicoleta Alice, Aflori, Laura, Vaida, Carmen, Ciobotaru, Oana Roxana, Aignatoaie, Mariana, Carp, Cristina Paula, Cobzaru, Isabelle, Mardare, Oana, Purcarin, Bianca, Tutunaru, Valentin, Ionita, Victor, Arustei, Mirela, Codita, Anisoara, Busuioc, Mihai, Chilinciuc, Ion, Ciobanu, Cristina, Belciu, Ioana, Tincu, Eugen, Blaj, Mihaela, Grosu, Ramona-Mihaela, Sandu, Gigel, Bruma, Dana, Corneci, Dan, Dutu, Madalina, Krepil, Adriana, Copaciu, Elena, Dumitrascu, Clementina Oana, Jemna, Ramona, Mihaescu, Florentina, Petre, Raluca, Tudor, Cristina, Ursache, Elena, Kulikov, Alexander, Lubnin, Andrey, Grigoryev, Evgeny, Pugachev, Stanislav, Protsenko, Deni, Tolmasov, Alexander, Hussain, Ayyaz, Ilyina, Yana, Kirov, Mikhail, Roshchina, Anna, Iurin, Aleksandr, Chazova, Elena, Dunay, Artem, Karelov, Alexey, Khvedelidze, Irina, Voldaeva, Olga, Belskiy, Vladislav, Dzhamullaev, Parvin, Grishkowez, Elena, Kretov, Vladimir, Levin, Valeriy, Molkov, Aleksandr, Puzanov, Sergey, Samoilenko, Aleksandr, Tchekulaev, Aleksandr, Tulupova, Valentina, Utkin, Ivan, Allorto, Nikki Leigh, Bishop, David Gray, Builu, Pierre Monji, Cairns, Carel, Dasrath, Ashish, De Wet, Jacque, Den Hoedt, Marielle, Grey, Ben, Hayes, Morgan Philip, Kã¼sel, Belinda Senta, Shangase, Nomcebo, Wise, Robert, Cacala, Sharon, Farina, Zane, Govindasamy, Vishendran, Kruse, Carl-Heinz, Lee, Carolyn, Marais, Leonard, Naidoo, Thinagrin Dhasarthun, Rajah, Chantal, Rodseth, Reitze Nil, Ryan, 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T., Loots, Emil, Madansein, Rajhmum, Madaree, Anil, Madiba, Thandinkosi E., Manzini, Vukani T., Mbuyisa, Mbali, Moodley, Rajan, Msomi, Mduduzi, Mukama, Innocent, Naidoo, Desigan, Naidoo, Rubeshan, Naidu, Tesuven K., Ntloko, Sindiswa, Padayachee, Eneshia, Padayachee, Lucelle, Phaff, Martijn, Pillay, Bala, Pillay, Desigan, Pillay, Lutchmee, Ramnarain, Anupa, Ramphal, Suren R., Ryan, Paul, Saloojee, Ahmed, Sebitloane, Motshedisi, Sigcu, Noluyolo, Taylor, Jenna L., Torborg, Alexandra, Visser, Linda, Anderson, Philip, Conradie, Alae, De Swardt, Mathew, De Villiers, Martin, Eikman, Johan, Liebenberg, Riaan, Mouton, Johan, Paton, Abbey, Van Der Merwe, Louwrence, Wilscott-Davids, Candice, Barrett, Wendy Joan, Bester, Marlet, De Beer, Johan, Geldenhuys, Jacque, Gouws, Hanni, Potgieter, Jan-Hendrik, Strydom, Magdel, Wilberforceturton, Edwin, Chetty, Rubendraj R., Chirkut, Subash, Cronje, Larissa, De Vasconcellos, Kim, Dube, Nokukhanya Z., Gama, N. Sibusiso, Green, Garyth M., Green-Thompson, Randolph, Kinoo, Suman Mewa, Kistnasami, Prenolin, Maharaj, Kapil, Moodley, Manogaran S., Mothae, Sibongile J., Naidoo, Ruvashni, Noorbhai, M. 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Andrew, Foot, Jayne, Large, Simon, Claxton, Andrew, Netke, Bhagyashree, Armstrong, Richard, Calderwood, Claire, Kwok, Andy, Mohr, Otto, Oyeniyi, Peter, Patnaik, Lisa, Post, Benjamin, Ali, Sarah, Arshad, Homa, Baker, Gerard, Brenner, Laura, Brincat, Maximilian, Brunswicker, Annemarie, Cox, Hannah, Cozar, Octavian Ionut, Cheong, Edward, Durst, Alexander, Fengas, Lior, Flatt, Jim, Glister, Georgina, Narwani, Vishal, Photi, Evangelo, Rankin, Adeline, Rosbergen, Melissa, Tan, Mark, Beaton, Ceri, Horn, Rachel, Hunt, Jane, Rousseau, Guy, Stancombe, Lucia, Absar, Mohammed, Allsop, Joanne, Drinkwater, Zoe, Hodgkiss, Tracey, Smith, Kirsty, Brown, Jamie, Alexander-Sefre, Farhad, Campey, Lorraine, Dudgeon, Lucy, Hall, Kathryn, Hitchcock, Rachael, James, Lynne, Smith, Kate, Winstone, Ulrika, Ahmad, Norfaizan, Bauchmuller, Kri, Harrison, Jonathan, Jeffery, Holly, Miller, Duncan, Pinder, Angela, Pothuneedi, Sailaja, Rosser, Jonathan, Sanghera, Sumayer, Swift, Diane, Walker, Rachel, Bester, Delia, 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Stevens, Alice, Waweru, Peter, Yeh, Erlinda, Bodansky, Daniella, Evans, Adam, Kleopoulos, Steven, Maril, Robert, Mathney, Edward, Sanchez, Angela, Tinuoye, Elizabeth, Bateman, Brian, Eng, Kristen, Jiang, Ning, Ladha, Karim, Needleman, Joseph, Chen, Lee-Lynn, Lane, Rondall, Robinowitz, David, Ghushe, Neil, Irshad, Mariam, O'Connor, John, Patel, Samir, Myles, Paul, Hiesmayr, Michael, Fang, Xiangming, Slim, Karem, Sander, Michael, Koulenti, Despoina, Chan, Mathew, Abbas, Muntadhar, Sivasakthi, Datin, Osinaike, Tunde, Matos, Ricardo, Grigoras, Ioana, Hubner, Martyn, Other departments, The International Surgical Outcomes Study, Group, Beretta, L, Landoni, G, and Zangrillo, A
Subjects: Critical Care/utilization, Postoperative Complications/epidemiology, Male, cohort studies, critical care/utilisation, operative/mortality, postoperative care/methods, postoperative care/statistics and numerical data, surgery, surgical procedures, Global Health, law.invention, surgery, Postoperative Complications, 0302 clinical medicine, Correction Notice, cohort studies, Anesthesiology, 030202 anesthesiology, law, cohort studiescritical care/utilisationoperative/mortalitypostoperative care/methodspostoperative care/statistics and numerical datasurgerysurgical procedures, 80 and over, Hospital Mortality, Prospective Studies, 030212 general & internal medicine, Prospective cohort study, Aged, 80 and over, International Surgical Outcomes Study group, Elective Surgical Procedures/mortality, HCC NEF, Middle Aged, Elective Surgical Procedures/adverse effects, Intensive care unit, surgical procedure, 3. Good health, Treatment Outcome, Length of Stay/statistics & numerical data, operative/mortality, Elective Surgical Procedures, Female, Elective Surgical Procedure, Life Sciences & Biomedicine, Cohort study, Adult, medicine.medical_specialty, Adolescent, Critical Care, critical care/utilisation, postoperative care/methods, postoperative care/statistics and numerical data, surgical procedures, Aged, Humans, Length of Stay, Poverty, Socioeconomic Factors, Young Adult, Anesthesiology and Pain Medicine, NO, Clinical Practice, 03 medical and health sciences, Intensive care, Journal Article, medicine, postoperative care/method, Elective surgery, Science & Technology, business.industry, Postoperative complication, 030208 emergency & critical care medicine, 1103 Clinical Sciences, Surgery, Global Health/statistics & numerical data, Emergency medicine, business, cohort studie
Abstract: Background:As global initiatives increase patient access to surgical treatments, there remains a need to understand the adverse effects of surgery and define appropriate levels of perioperative care. Methods:We designed a prospective international 7-day cohort study of outcomes following elective adult inpatient surgery in 27 countries. The primary outcome was in-hospital complications. Secondary outcomes were death following a complication (failure to rescue) and death in hospital. Process measures were admission to critical care immediately after surgery or to treat a complication and duration of hospital stay. A single definition of critical care was used for all countries. Results:A total of 474 hospitals in 19 high-, 7 middle- and 1 low-income country were included in the primary analysis. Data included 44814 patients with a median hospital stay of 4 (range 2–7) days. A total of 7508 patients (16.8%) developed one or more postoperative complication and 207 died (0.5%). The overall mortality among patients who developed complications was 2.8%. Mortality following complications ranged from 2.4% for pulmonary embolism to 43.9% for cardiac arrest. A total of 4360 (9.7%) patients were admitted to a critical care unit as routine immediately after surgery, of whom 2198 (50.4%) developed a complication, with 105 (2.4%) deaths. A total of 1233 patients (16.4%) were admitted to a critical care unit to treat complications, with 119 (9.7%) deaths. Despite lower baseline risk, outcomes were similar in low- and middle-income compared with high-income countries. Conclusions:Poor patient outcomes are common after inpatient surgery. Global initiatives to increase access to surgical treatments should also address the need for safe perioperative care. Study registration:ISRCTN51817007
Published: 2016

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