57 results on '"A. Deodati"'
Search Results
2. The exposure to uteroplacental insufficiency is associated with activation of unfolded protein response in postnatal life.
- Author
-
Annalisa Deodati, Josepmaría Argemí, Daniela Germani, Antonella Puglianiello, Anna Alisi, Cristiano De Stefanis, Roberto Ferrero, Valerio Nobili, Tomás Aragón, and Stefano Cianfarani
- Subjects
Medicine ,Science - Abstract
Early life events are associated with the susceptibility to chronic diseases in adult life. Perturbations of endoplasmic reticulum (ER) homeostasis activate the unfolded protein response (UPR), which contributes to the development of metabolic alterations. Our aim was to evaluate liver UPR in an animal model of intrauterine growth restriction (IUGR). A significantly increased expression of X-box binding protein-1 spliced (XBP1s) mRNA (p
- Published
- 2018
- Full Text
- View/download PDF
3. Expression of insulin-like growth factor I and its receptor in the liver of children with biopsy-proven NAFLD.
- Author
-
Anna Alisi, Valentina Pampanini, Cristiano De Stefanis, Nadia Panera, Annalisa Deodati, Valerio Nobili, and Stefano Cianfarani
- Subjects
Medicine ,Science - Abstract
BACKGROUND AND AIMS:Nonalcoholic fatty liver disease is one of the major complications of obesity, occurring already in pediatric age. Insulin like growth factor-I has been proposed as a potential therapeutic agent for its beneficial effect in experimental liver fibrosis. The aim of this work was to investigate the expression of insulin-like growth factor-I and its receptor in the liver of children with biopsy-proven nonalcoholic fatty liver disease and relate it to liver histological features. METHODS:45 obese children and adolescents (14 females and 31 males) with nonalcoholic fatty liver disease were included. Insulin like growth factor-I and its receptor expression was evaluated in liver tissue by immunofluorescence and qPCR. RESULTS:The expression of insulin like growth factor-I and its receptor were significantly related to fibrosis and were higher in children with stage 3 fibrosis compared to stage 1 and 2 (p
- Published
- 2018
- Full Text
- View/download PDF
4. Duplex Ultrasound Screening for Deep and Superficial Vein Thrombosis in COVID‐19 Patients
- Author
-
Francesco Deodati, Cristina Marcelo, Beatriz Álvarez, María Mateos, Lucía Ordieres, Yale Tung-Chen, Ruth Calderón, Javier Marco, and Aranzazu Castellano
- Subjects
Male ,medicine.medical_specialty ,Duplex ultrasonography ,Superficial vein thrombosis ,medicine.medical_treatment ,Coronavirus Disease 2019 (COVID‐19) ,deep vein thrombosis ,Oxygen therapy ,superficial vein thrombosis ,medicine ,Humans ,duplex ultrasonography ,Radiology, Nuclear Medicine and imaging ,Medical history ,Retrospective Studies ,Original Research ,Venous Thrombosis ,Ultrasonography, Doppler, Duplex ,Radiological and Ultrasound Technology ,business.industry ,Incidence (epidemiology) ,COVID-19 ,Venous Thromboembolism ,Heparin, Low-Molecular-Weight ,Middle Aged ,medicine.disease ,Thrombosis ,Surgery ,medicine.vein ,Duplex (building) ,Female ,Posterior tibial vein ,business ,Severe Acute Respiratory Syndrome Coronavirus 2 (SARS‐CoV‐2) - Abstract
Background There is growing evidence regarding the venous thromboembolic (VTE) pathophysiology of coronavirus disease 2019 (COVID-19). Several studies have reported varying incidences of this disease. Objectives The main purpose of this study was to determine the real incidence of deep or superficial vein thrombosis in COVID-19. The study also aimed to identify risk and protective factors for VTE. Methods Patients were consecutively enrolled and assessed with a bilateral Duplex ultrasonography of lower limbs during hospitalization. The exam was repeated weekly until discharge, and then follow-up for 1 month. Results Two-hundred and thirty-three patients were enrolled. Mean age was 54.4 years (SD 12.7) and 47.8% were female. About 127 patients (54.5%) had comorbidities. At enrollment, patients were normotensive and had normal saturation (95.6%-SD 1.6, with a respiratory rate of 19.1 rpm-SD 4.0), with 130 needing at least supplementary oxygen therapy (55.8%). About 147 patients (63.1%) had at least 1 Duplex ultrasonography study performed and 1.7% had 5 or more studies. One patient had a distal posterior tibial vein thrombosis, which showed signs of chronicity and was congruent with the patient history. Therefore, the incidence of thrombotic events was nearly zero. Discussion Our study results suggest that performing a Duplex Ultrasonography screening protocol in stable COVID-19 patient populations, who may need hospitalization but are without symptoms of vein thrombosis, is not founded. We presumably emphasize the advantage of using intermediate LMWH doses as well as early walking in COVID-19 patients.
- Published
- 2021
5. Post-traumatic stress disorder (PTSD) in parents of children with type 1 diabetes during Covid-19 pandemic
- Author
-
V. Pampanini, N. Rapini, C. Carducci, Riccardo Schiaffini, Stefano Cianfarani, and Annalisa Deodati
- Subjects
Adult ,Male ,Coronavirus disease 2019 (COVID-19) ,Mothers ,Pediatrics ,RJ1-570 ,Fight-or-flight response ,Cohort Studies ,Stress Disorders, Post-Traumatic ,Intrusion ,Fathers ,Surveys and Questionnaires ,Pandemic ,medicine ,Prevalence ,Humans ,Child ,Post-traumatic stress disorder (PTSD) ,Type 1 diabetes ,Post-traumatic stress disorder ,business.industry ,SARS-CoV-2 ,Research ,Middle Aged ,medicine.disease ,Settore MED/38 ,Diabetes Mellitus, Type 1 ,Italy ,Communicable Disease Control ,Female ,Symptom Assessment ,business ,Covid-19 ,Psychosocial ,Pediatric diabetes ,Clinical psychology ,Cohort study - Abstract
Introduction The Post-traumatic Stress Disorder (PTSD) is a group of persistent psychological and physiological symptoms due to a traumatic, severe, event. Only few studies focused on the effects of Covid-19 on psychosocial outcomes in children with Type 1 Diabetes (T1D) and their parents. Aim of the study The aim of this study was to evaluate the presence PTSD in parents of children with T1D during COVID-19 pandemic lockdown. Patients and methods In the period between March and May 2020 we submitted the “Impact of Event Scale – Revised” (IES-R) questionnaire to the parents of 34 children with Type 1 Diabetes, asking them to express their emotions about the ongoing Covid-19 pandemic. Results A total of thirty mothers (mean age 43.0 ± 4.2 years) and 25 fathers (mean age 45.6 ± 5.9 years) participated in the survey and completed the questionnaires. 29.1% of parents had a score that allows to define a clinically relevant level of PTSD; ten mothers and 6 fathers had a PTSD clinically relevant score, corresponding, respectively, to 28.4 and 24% of total mothers and fathers. Finally, mothers and fathers, both express PTSD symptoms mainly in the form of intrusion and hyperarousal. Conclusions The present study confirms a high prevalence symptoms related to PTSD in mothers and fathers of children with Type 1 Diabetes. We believe that psychosocial outcomes of the COVID-19 pandemic should be taken into account in the planning of the next future assistance for children with T1D.
- Published
- 2021
6. Prevalence of copy number variants (CNVs) and rhGH treatment efficacy in an Italian cohort of children born small for gestational age (SGA) with persistent short stature associated with a complex clinical phenotype
- Author
-
Giorgia Catino, Marco Cappa, E. Sallicandro, Sara Loddo, Stefano Cianfarani, Annalisa Deodati, Mafalda Mucciolo, Antonio Novelli, F. Verdecchia, and Elena Inzaghi
- Subjects
Male ,congenital, hereditary, and neonatal diseases and abnormalities ,Pediatrics ,medicine.medical_specialty ,DNA Copy Number Variations ,Endocrinology, Diabetes and Metabolism ,Rhgh treatment ,Dwarfism ,Gestational Age ,Growth Hormone-Releasing Hormone ,Short stature ,Cohort Studies ,Endocrinology ,Genotype ,Female patient ,Prevalence ,medicine ,Humans ,Copy-number variation ,Clinical phenotype ,Growth hormone ,Genetic Association Studies ,Retrospective Studies ,CNVs ,SGA ,business.industry ,Infant, Newborn ,medicine.disease ,Settore MED/38 ,Peptide Fragments ,Phenotype ,Treatment Outcome ,Italy ,Infant, Small for Gestational Age ,Cohort ,Small for gestational age ,Female ,medicine.symptom ,business - Abstract
Multiple factors influence intrauterine growth and lead to low birth sizes. The impact of genetic alterations on both pre- and post-natal growth is still largely unknown. The aim of this study was to investigate the prevalence of CNVs in an Italian cohort of SGA children with persistent short stature and complex clinical phenotype. rhGH treatment efficacy was evaluated according to the different genotypes. Twenty-four SGA children (10F/14M) with persistent short stature associated with dysmorphic features and/or developmental delay underwent CNV evaluation. CNVs were present in 14/24 (58%) SGA children. Six patients had a microdeletion involving the following regions: 3q24q25.1, 8p21.2p12, 15q26, 19q13.11, 20q11.21q12, 22q11.2. In three females, the same microdeletion involving 17p13.3 region was identified. In two different patients, two microduplications involving 10q21.3 and Xp11.3 region were observed. A further female patient showed both an 11q12.1 and an Xq27.1 microduplication, inherited from her mother and from her father, respectively. In a boy, the presence of a 12p13.33 microdeletion and a 19q13.43 microduplication was found. GH treatment efficacy, expressed by height gain and height velocity in the first 12 months of therapy, was similar in subjects with and without CNVs. These results show that pathogenic CNVs are common in SGA children with short stature associated with additional clinical features. Interestingly, the involvement of 17p13.3 region occurs with a relative high frequency, suggesting that genes located in this region could play a key role in pre- and post-natal growth. rhGH therapy has similar efficacy in the short term whether CNVs are present or not.
- Published
- 2021
7. Mechanisms of acute hypercalcemia in pediatric patients following the interruption of Denosumab
- Author
-
Michela Rossi, A. Del Fattore, Giulia Battafarano, E Levtchenko, Annalisa Deodati, Marco Cappa, H Segers, Graziamaria Ubertini, and Danilo Fintini
- Subjects
Oncology ,Male ,medicine.medical_specialty ,Adolescent ,Endocrinology, Diabetes and Metabolism ,Osteoclast precursor ,Primary Cell Culture ,Osteoclasts ,Peripheral blood mononuclear cell ,Endocrinology ,Belgium ,Osteoclast ,Granuloma, Giant Cell ,Internal medicine ,Medicine ,Humans ,Mandibular Diseases ,Receptor ,Child ,Cells, Cultured ,biology ,business.industry ,Settore MED/38 ,Discontinuation ,Bone Cysts, Aneurysmal ,Denosumab ,medicine.anatomical_structure ,Integrin alpha M ,Withholding Treatment ,Case-Control Studies ,Monoclonal ,Acute Disease ,biology.protein ,Hypercalcemia ,Leukocytes, Mononuclear ,Antibody ,business ,medicine.drug ,Signal Transduction - Abstract
Denosumab is a fully human monoclonal anti-RANK-L antibody that is clinically used to counteract the bone loss induced by exacerbated osteoclast activity. Indeed, its binding to RANK-L prevents the interaction RANK-L/receptor RANK that is essential for osteoclastogenesis and bone resorbing activity. Although there are many medications available to treat bone loss diseases, including bisphosphonates, Denosumab is highly effective since it reduces the bone erosion. The use in pediatric patients is safe. However, some concerns are related to the interruption of the treatment. Indeed, in this study, we reported hypercalcemia in two pediatric patients and alterations of circulating osteoclast precursors. Peripheral Blood Mononuclear Cells (PBMC) were isolated from two pediatric patients with hypercalcemia after Denosumab interruption and from 10 controls. Cytofluorimetric analysis and in vitro osteoclastogenesis experiments were performed. Increase of CD16−CD14+CD11b+ cells was revealed in PBMC from patients reflecting the enhanced in vitro osteoclastogenesis. Our data suggest that precautions must be taken when Denosumab therapy is interrupted and gradual decrease of dose and/or timing of treatment should be performed. To prevent the onset of hypercalcemia that could be in the discontinuation phase, cytofluorimetric analysis of PBMC should be performed to evaluate osteoclast precursors.
- Published
- 2022
8. Corrigendum to: 'Bone density and body composition in small for gestational age children with adequate catch up growth: A preliminary retrospective case control study' [Bone-2021 Jul 14;153:116114. doi:10.1016/j.bone.2021.116114. Online ahead of print]
- Author
-
Danilo Fintini, Michela Mariani, Melania Manco, Sarah Bocchini, Elena Inzaghi, Marco Cappa, Wolfgang Högler, and Annalisa Deodati
- Subjects
Histology ,Bone density ,Physiology ,business.industry ,Endocrinology, Diabetes and Metabolism ,Case-control study ,Dentistry ,Medicine ,Small for gestational age ,business ,medicine.disease ,Settore MED/38 - Published
- 2021
9. Endocrinological features of a patient with 14q microdeletion and Dubowitz phenotype
- Author
-
Elena Inzaghi, Maria Elisa Amodeo, Stefano Cianfarani, and Annalisa Deodati
- Subjects
0301 basic medicine ,Delayed puberty ,Male ,Pediatrics ,medicine.medical_specialty ,Microcephaly ,Hormone Replacement Therapy ,Eczema ,Hypopituitarism ,030105 genetics & heredity ,QH426-470 ,Short stature ,Clinical Reports ,03 medical and health sciences ,Young Adult ,Intellectual Disability ,medicine ,Adrenal insufficiency ,Genetics ,Endocrine system ,Humans ,Dubowitz syndrome ,Molecular Biology ,Genetics (clinical) ,Growth Disorders ,Chromosomes, Human, Pair 14 ,Clinical Report ,business.industry ,Bone marrow failure ,Facies ,medicine.disease ,Settore MED/38 ,delayed puberty ,growth impairment ,030104 developmental biology ,Phenotype ,hypopituitarism ,Growth Hormone ,medicine.symptom ,Chromosome Deletion ,business ,GH therapy - Abstract
Background Dubowitz syndrome (DS) is a complex and rare condition characterized by postnatal growth retardation, microcephaly, short stature, mild developmental delay, facial dysmorphism, skin eruption and bone marrow failure. Though approximately 200 cases have been described so far, no specific genetic analysis, laboratory tests or radiological exams are available to confirm the diagnosis which is still based on clinical and facial features. Although short stature is a major feature of the syndrome, no endocrine alterations have been reported so far and scant data are available about the efficacy and safety of GH treatment in these patients. Methods A 13‐year‐old male patient was referred to our attention for short stature. Endocrinological evaluation including GH axis, adrenal and gonadal functions were assessed. aCGH was performed. Results 14q terminal microdeletion associated with Dubowitz phenotype was found. Endocrinological investigations revealed the presence of hypopituitarism which showed a satisfactory response to short‐term growth hormone therapy. The subject also started glucocorticoid replacement therapy. Disorders in pubertal progression and gonadal function were noted. Conclusions Dubowitz syndrome (DS) includes different clinical findings variably occurring. Subjects with a Dubowitz phenotype should be carefully monitored for endocrinological anomalies. The prompt recognition of potential life‐threatening endocrinological condition for example adrenal insufficiency is mandatory in order to start an adequate and early treatment., We report the endocrinological features of a male patient with 14q microdeletion and Dubowitz phenotype who showed a good response to short‐term GH therapy.
- Published
- 2021
10. Risk of Meningioma in European Patients Treated With Growth Hormone in Childhood: Results From the SAGhE Cohort
- Author
-
Anders Tidblad, Muriel Thomas, Lars Sävendahl, Christa E. Flück, Dominique Beckers, Peter E. Clayton, Aysha J. Khan, Joël Coste, Stefano Cianfarani, Annalisa Deodati, Jean-Claude Carel, Roland Pfäffle, Sally Tollerfield, Gladys R J Zandwijken, Emmanuel Ecosse, Grit Sommer, Anita C. S. Hokken-Koelega, Gary Butler, Claudia E. Kuehni, Anthony J. Swerdlow, Rosie Cooke, Wieland Kiess, and Pediatrics
- Subjects
Male ,Pediatrics ,Endocrinology, Diabetes and Metabolism ,Clinical Biochemistry ,Biochemistry ,0302 clinical medicine ,Endocrinology ,Neoplasms ,Meningeal Neoplasms ,Registries ,Child ,Growth Disorders ,Cumulative dose ,Adolescent ,Adult ,Child, Preschool ,Cranial Irradiation ,Europe ,Female ,Follow-Up Studies ,Human Growth Hormone ,Humans ,Incidence ,Infant ,Infant, Newborn ,Meningioma ,Neoplasms, Second Primary ,Recombinant Proteins ,Risk Assessment ,Young Adult ,Incidence (epidemiology) ,Settore MED/38 ,3. Good health ,Growth, Growth Hormone, and Growth Factors ,Second Primary ,030220 oncology & carcinogenesis ,Cohort ,Cohort study ,medicine.medical_specialty ,030209 endocrinology & metabolism ,Context (language use) ,610 Medicine & health ,Malignancy ,03 medical and health sciences ,360 Social problems & social services ,Internal medicine ,medicine ,Preschool ,Clinical Research Articles ,business.industry ,Biochemistry (medical) ,Cancer ,medicine.disease ,Newborn ,business - Abstract
Context There has been concern that GH treatment of children might increase meningioma risk. Results of published studies have been inconsistent and limited. Objective To examine meningioma risks in relation to GH treatment. Design Cohort study with follow-up via cancer registries and other registers. Setting Population-based. Patients A cohort of 10,403 patients treated in childhood with recombinant GH in five European countries since this treatment was first used in 1984. Expected rates from national cancer registration statistics. Main Outcome Measures Risk of meningioma incidence. Results During follow-up, 38 meningiomas occurred. Meningioma risk was greatly raised in the cohort overall [standardized incidence ratio (SIR) = 75.4; 95% CI: 54.9 to 103.6], as a consequence of high risk in subjects who had received radiotherapy for underlying malignancy (SIR = 658.4; 95% CI: 460.4 to 941.7). Risk was not significantly raised in patients who did not receive radiotherapy. Risk in radiotherapy-treated patients was not significantly related to mean daily dose of GH, duration of GH treatment, or cumulative dose of GH. Conclusions Our data add to evidence of very high risk of meningioma in patients treated in childhood with GH after cranial radiotherapy, but suggest that GH may not affect radiotherapy-related risk, and that there is no material raised risk of meningioma in GH-treated patients who did not receive radiotherapy., In a five-country cohort of 10,403 patients treated with recombinant growth hormone, meningioma risk was greatly raised in relation to radiotherapy, but not apparently related to growth hormone.
- Published
- 2019
11. Targeted Searches of the Electronic Health Record and Genomics Identify an Etiology in Three Patients with Short Stature and High IGF-I Levels
- Author
-
Catalina Cabrera-Salcedo, Adda Grimberg, Andrew Dauber, Joel N. Hirschhorn, Melissa Andrew, Diego Campos, Leah Tyzinski, Annalisa Deodati, Guillaume Labilloy, Colin P. Hawkes, Vivian Hwa, and Amalia Feld
- Subjects
Exome sequencing ,Male ,Pediatrics ,medicine.medical_specialty ,Adolescent ,Referral ,Bioinformatics ,Endocrinology, Diabetes and Metabolism ,Mutation, Missense ,030209 endocrinology & metabolism ,Genomics ,Short stature ,Article ,Whole Exome Sequencing ,Receptor, IGF Type 1 ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,IGF1R ,Electronic health record ,Health care ,medicine ,Electronic Health Records ,Humans ,Insulin-Like Growth Factor I ,Child ,Growth Disorders ,030219 obstetrics & reproductive medicine ,business.industry ,Settore MED/38 ,Body Height ,DNA-Binding Proteins ,Cross-Sectional Studies ,HEK293 Cells ,Phenotype ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Etiology ,Female ,medicine.symptom ,business ,Cohort study - Abstract
Introduction: Short stature is one of the most common reasons for referral to a pediatric endocrinologist and can result from many etiologies. However, many patients with short stature do not receive a definitive diagnosis. Objective: To ascertain whether integrating targeted bioinformatics searches of electronic health records (EHRs) combined with genomic studies could identify patients with previously undiagnosed rare genetic etiologies of short stature. We focused on a specific rare phenotypic subgroup: patients with short stature and elevated IGF-I levels. Methods: We performed a cross-sectional cohort study at three large academic pediatric healthcare networks. Eligible subjects included children with heights below –2 SD, IGF-I levels >90th percentile, and no known etiology for short stature. We performed a search of the EHRs to identify eligible patients. Patients were then recruited for phenotyping followed by exome sequencing and in vitro assays of IGF1R function. Results: A total of 234 patients were identified by the bioinformatics algorithm with 39 deemed eligible after manual review (17%). Of those, 9 were successfully recruited. A genetic etiology was identified in 3 of the 9 patients including 2 novel variants in IGF1R and a de novo variant in CHD2. In vitro studies supported the pathogenicity of the IGF1R variants. Conclusions: This study provides proof of principle that patients with rare phenotypic subgroups can be identified based on discrete data elements in the EHRs. Although limitations exist to fully automating this approach, these searches may help find patients with previously unidentified rare genetic disorders.
- Published
- 2019
12. Metabolic, reproductive and thyroid effects of bis(2-ethylhexyl) phthalate (DEHP) orally administered to male and female juvenile rats at dose levels derived from children biomonitoring study
- Author
-
Amalia Gastaldelli, Mauro Valeri, Francesca Maranghi, Luca Busani, Andrea Martinelli, Cinzia La Rocca, Sabrina Tait, Annalisa Deodati, Laura Narciso, and Roberta Tassinari
- Subjects
0301 basic medicine ,Male ,endocrine system ,Thyroid Gland ,Physiology ,Administration, Oral ,Toxicology ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Thyroid-stimulating hormone ,Plasticizers ,NOAEL/LOAEL ,Diethylhexyl Phthalate ,Plasticizer ,medicine ,Endocrine system ,Animals ,Humans ,Juvenile animal ,Sexual Maturation ,Benchmark dose ,Child ,Testosterone ,Bis(2-ethylhexyl) phthalate ,Dose-Response Relationship, Drug ,business.industry ,Reproduction ,Thyroid ,Phthalate ,Age Factors ,Endocrine disrupter ,Peri-pubertal ,Settore MED/38 ,Rats ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,Animals, Newborn ,Sex-related effects ,Female ,business ,030217 neurology & neurosurgery ,Hormone ,Biological Monitoring - Abstract
Bis(2-ethylhexyl) phthalate (DEHP) is a plasticizer used in several items, non-covalently bound to plastics and easily released, since metabolites were found in human matrices. DEHP is an endocrine disrupter and children are particularly vulnerable and susceptible to DEHP effects due to higher exposure levels and developmental stage. A juvenile toxicity study was performed to identify DEHP hazard and mode of action in Sprague-Dawley rats of both sexes during peri-pubertal period - corresponding to childhood phase - from weaning, post-natal day (PND) 23, to full sexual maturity (PND60); the dose levels of 0, 9, 21 and 48 mg/kg bw/day were derived from LIFE PERSUADED biomonitoring study in children. DEHP was administered by gavage for 28 days (5 days/week); timing of preputial separation and vaginal opening was observed during treatment. Histopathological analysis was performed on: adrenals, spleen, liver, thyroid and reproductive organs. The following serum biomarkers were assessed: estradiol, testosterone, anti-Mullerian hormone, tetraiodothyronine, thyroid stimulating hormone, adiponectin and leptin. Gene expression on hypothalamic-pituitary area was focused on follicle stimulating, luteinizing, and thyroid stimulating hormones. The results showed that main targets of DEHP during juvenile period were liver and metabolic system in both sexes, while sex-specific effects were recorded in reproductive system (male rats) and in thyroid (female rats). DEHP exposure during peri-pubertal period at dose levels derived from biomonitoring study in children can induce sex-specific imbalances identifying the juvenile animal model as a sound tool to identify hazards for a reliable risk assessment targeted to children.
- Published
- 2021
13. Differences between Transient Neonatal Diabetes Mellitus Subtypes can Guide Diagnosis and Therapy
- Author
-
Riccardo Bonfanti, Dario Iafusco, Ivana Rabbone, Giacomo Diedenhofen, Carla Bizzarri, Patrizia Ippolita Patera, Petra Reinstadler, Francesco Costantino, Valeria Calcaterra, Lorenzo Iughetti, Silvia Savastio, Anna Favia, Francesca Cardella, Donatella Lo Presti, Ylenia Girtler, Sarah Rabbiosi, Giuseppe D’Annunzio, Angela Zanfardino, Alessia Piscopo, Francesca Casaburo, Letizia Pintomalli, Lucia Russo, Valeria Grasso, Nicola Minuto, Mafalda Mucciolo, Antonio Novelli, Antonella Marucci, Barbara Piccini, Sonia Toni, Francesca Silvestri, Paola Carrera, Andrea Rigamonti, Giulio Frontino, Michela Trada, Davide Tinti, Maurizio Delvecchio, Novella Rapini, Riccardo Schiaffini, Corrado Mammì, Fabrizio Barbetti, Monica Aloe, Simona Amadeo, Claudia Arnaldi, Marta Bassi, Luciano Beccaria, Marzia Benelli, Giulia Maria Berioloi, Enrica Bertelli, Martina Biagioni, Adriana Bobbio, Stefano Boccato, Oriana Bologna, Franco Bontempi, Clara Bonura, Giulia Bracciolini, Claudia Brufani, Patrizia Bruzzi, Pietro Buono, Roberta Cardani, Giuliana Cardinale, Alberto Casertano, Maria Cristina Castiglione, Vittoria Cauvin, Valentino Cherubini, Franco Chiarelli, Giovanni Chiari, Stefano Cianfarani, Dante Cirillo, Felice Citriniti, Susanna Coccioli, Anna Cogliardi, Santino Confetto, Giovanna Contreas, Anna Corò, Elisa Corsini, Nicoletta Cresta, Fiorella De Berardinis, Valeria De Donno, Giampaolo De Filippo, Rosaria De Marco, Annalisa Deodati, Elena Faleschini, Valentina Fattorusso, Valeria Favalli, Barbara Felappi, Lucia Ferrito, Graziella Fichera, Franco Fontana, Elena Fornari, Roberto Franceschi, Francesca Franco, Adriana Franzese, Anna Paola Frongia, Alberto Gaiero, Francesco Gallo, Luigi Gargantini, Elisa Giani, Chiara Giorgetti, Giulia Bianchi, Vanna Graziani, Antonella Gualtieri, Monica Guasti, Gennaro Iannicelli, Antonio Iannilli, Ignaccolo Giovanna, Dario Ingletto, Stefania Innaurato, Elena Inzaghi, Brunella Iovane, Peter Kaufmann, Alfonso La Loggia, Rosa Lapolla, Anna Lasagni, Nicola Lazzaro, Lorenzo Lenzi, Riccardo Lera, Gabriella Levantini, Fortunato Lombardo, Antonella Lonero, Silvia Longhi, Sonia Lucchesi, Lucia Paola Guerraggio, Sergio Lucieri, Patrizia Macellaro, Claudio Maffeis, Bendetta Mainetti, Giulio Maltoni, Chiara Mameli, Francesco Mammì, Maria Luisa Manca-Bitti, Melania Manco, Monica Marino, Matteo Mariano, Marco Marigliano, Alberto Marsciani, Costanzo Mastrangelo, Maria Cristina Matteoli, Elena Mazzali, Franco Meschi, Antonella MIgliaccio, Anita Morandi, Gianfranco Morganti, Enza Mozzillo, Gianluca Musolino, Rosa Nugnes, Federica Ortolani, Daniela Pardi, Filomena Pascarella, Stefano Passanisi, Annalisa Pedini, Cristina Pennati, Angelo Perrotta, Sonia Peruzzi, Paola Peverelli, Giulia Pezzino, Anita Claudia Piona, Gavina Piredda, Carmelo Pistone, Elena Prandi, Barbara Pedieri, Procolo Di Bonito, Anna Pulcina, Maria Quinci, Emioli Randazzo, Rossella Ricciardi, Carlo Ripoli, Rosanna Roppolo, Irene Rutigliano, Alberto Sabbio, Silvana salardi, Alessandro Salvatoni, Anna Saporiti, Rita Sardi, Mariapiera Scanu, Andrea Scaramuzza, Eleonardo Schiven, Andrea Secco, Linda Sessa, Paola Sogno Valin, Silvia Sordelli, Luisa Spallino, Stefano Stagi, Filomena Stamati, Tosca Suprani, Valentina Talarico, Tiziana Timapanaro, Antonella Tirendi, Letizia Tomaselli, Gianluca Tornese, Adolfo Andrea Trettene, Stefano Tumini, Giuliana Valerio, Claudia Ventrici, Matteo Viscardi, Silvana Zaffani, Maria Zampolli, Giorgio Zanette, Clara Zecchino, Maria Antonietta Zedda, Silvia Zonca, Stefano Zucchini, Bonfanti, R., Iafusco, D., Rabbone, I., Diedenhofen, G., Bizzarri, C., Patera, P. I., Reinstadler, P., Costantino, F., Calcaterra, V., Iughetti, L., Savastio, S., Favia, A., Cardella, F., Presti, D. L., Girtler, Y., Rabbiosi, S., D'Annunzio, G., Zanfardino, A., Piscopo, A., Casaburo, F., Pintomalli, L., Russo, L., Grasso, V., Minuto, N., Mucciolo, M., Novelli, A., Marucci, A., Piccini, B., Toni, S., Silvestri, F., Carrera, P., Rigamonti, A., Frontino, G., Trada, M., Tinti, D., Delvecchio, M., Rapini, N., Schiaffini, R., Mammi, C., and Barbetti, F.
- Subjects
Proband ,Male ,Pediatrics ,Potassium Channels ,Endocrinology, Diabetes and Metabolism ,Datasets as Topic ,Diagnosis, Differential ,Diagnostic Techniques, Endocrine ,Female ,Humans ,Infant ,Infant, Newborn ,Italy ,Mutation ,Potassium Channels, Inwardly Rectifying ,Remission Induction ,Retrospective Studies ,Sulfonylurea Receptors ,Diabetes Mellitus ,Infant, Newborn, Diseases ,Diseases ,Gastroenterology ,Diabetes mellitus genetics ,Endocrinology ,Settore MED/13 ,Retrospective Studie ,Diagnosis ,Medicine ,Endocrine pancreas, Transient Neonatal Diabetes Mellitus, 6q24 TNDM, KATP TNDM, Sulfonylureas ,Sulfonylureas ,Sulfonylurea Receptor ,biology ,Diabetes Mellitu ,General Medicine ,Metformin ,Inwardly Rectifying ,Settore MED/03 ,6q24 TNDM ,medicine.symptom ,Endocrine ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug ,Human ,endocrine system ,medicine.medical_specialty ,KATP TNDM ,ABCC8 ,Transient Neonatal Diabetes Mellitus ,Internal medicine ,Diabetes mellitus ,Macroglossia ,Endocrine pancreas ,business.industry ,medicine.disease ,Newborn ,Diagnostic Techniques ,Transient neonatal diabetes mellitus ,Differential ,biology.protein ,Sulfonylurea receptor ,business - Abstract
Objective Transient neonatal diabetes mellitus (TNDM) is caused by activating mutations in ABCC8 and KCNJ11 genes (KATP/TNDM) or by chromosome 6q24 abnormalities (6q24/TNDM). We wanted to assess whether these different genetic aetiologies result in distinct clinical features. Design Retrospective analysis of the Italian data set of patients with TNDM. Methods Clinical features and treatment of 22 KATP/TNDM patients and 12 6q24/TNDM patients were compared. Results Fourteen KATP/TNDM probands had a carrier parent with abnormal glucose values, four patients with 6q24 showed macroglossia and/or umbilical hernia. Median age at diabetes onset and birth weight were lower in patients with 6q24 (1 week; −2.27 SD) than those with KATP mutations (4.0 weeks; −1.04 SD) (P = 0.009 and P = 0.007, respectively). Median time to remission was longer in KATP/TNDM than 6q24/TNDM (21.5 weeks vs 12 weeks) (P = 0.002). Two KATP/TNDM patients entered diabetes remission without pharmacological therapy. A proband with the ABCC8/L225P variant previously associated with permanent neonatal diabetes entered 7-year long remission after 1 year of sulfonylurea therapy. Seven diabetic individuals with KATP mutations were successfully treated with sulfonylurea monotherapy; four cases with relapsing 6q24/TNDM were treated with insulin, metformin or combination therapy. Conclusions If TNDM is suspected, KATP genes should be analyzed first with the exception of patients with macroglossia and/or umbilical hernia. Remission of diabetes without pharmacological therapy should not preclude genetic analysis. Early treatment with sulfonylurea may induce long-lasting remission of diabetes in patients with KATP mutations associated with PNDM. Adult patients carrying KATP/TNDM mutations respond favourably to sulfonylurea monotherapy.
- Published
- 2021
14. Inappropriate antibiotic use in the COVID-19 era: Factors associated with inappropriate prescribing and secondary complications. Analysis of the registry SEMI-COVID
- Author
-
Calderón Parra, Jorge, Muiño Míguez, Antonio, Bendala Estrada, Alejandro David, Ramos Martínez, Antonio, Muñez Rubio, Elena, Fernández Carracedo, Eduardo, Tejada Montes, Javier, Rubio-Rivas, Manuel, Arnalich Fernández, Francisco, Beato Pérez, Jose Luis, García Bruñén, Jose Miguel, Corral-Beamonte, Esther del, Pesqueira Fontan, Paula Maria, Carmona, Maria Del Mar, Fernández-Madera Martínez, Rosa, González García, Andrés, Salazar Mosteiro, Cristina, Tuñón de Almeida, Carlota, González Moraleja, Julio, Deodati, Francesco, Martín Escalante, María Dolores, Asensio Tomás, María Luisa, Gómez Huelgas, Ricardo, Casas Rojo, José, Millán Núñez-Cortés, Jesús, and For The Semi-covid-19 Network
- Subjects
0301 basic medicine ,Male ,Viral Diseases ,Epidemiology ,Physiology ,Antibiotics ,Fevers ,Inappropriate Prescribing ,Comorbidity ,medicine.disease_cause ,Medical Conditions ,0302 clinical medicine ,Risk Factors ,Medicine and Health Sciences ,Coughing ,Odds Ratio ,Medicine ,030212 general & internal medicine ,Registries ,Multidisciplinary ,Antimicrobials ,Drugs ,Acute Kidney Injury ,Middle Aged ,Anti-Bacterial Agents ,Infectious Diseases ,C-Reactive Protein ,Superinfection ,Cohort ,Female ,Research Article ,medicine.medical_specialty ,Fever ,medicine.drug_class ,Science ,Immunology ,030106 microbiology ,Pharmacy practice ,Microbiology ,Autoimmune Diseases ,03 medical and health sciences ,Signs and Symptoms ,Antibiotic resistance ,Microbial Control ,Internal medicine ,Humans ,Medical prescription ,Aged ,Retrospective Studies ,Atenció farmacèutica ,Pharmacology ,business.industry ,SARS-CoV-2 ,Pharmacy--Practice ,Biology and Life Sciences ,COVID-19 ,Covid 19 ,Retrospective cohort study ,Odds ratio ,medicine.disease ,Dyspnea ,Logistic Models ,Cough ,Antibiotic Resistance ,Medical Risk Factors ,Clinical Immunology ,Antimicrobial Resistance ,Clinical Medicine ,Physiological Processes ,business - Abstract
Background Most patients with COVID-19 receive antibiotics despite the fact that bacterial co-infections are rare. This can lead to increased complications, including antibacterial resistance. We aim to analyze risk factors for inappropriate antibiotic prescription in these patients and describe possible complications arising from their use. Methods The SEMI-COVID-19 Registry is a multicenter, retrospective patient cohort. Patients with antibiotic were divided into two groups according to appropriate or inappropriate prescription, depending on whether the patient fulfill any criteria for its use. Comparison was made by means of multilevel logistic regression analysis. Possible complications of antibiotic use were also identified. Results Out of 13,932 patients, 3047 (21.6%) were prescribed no antibiotics, 6116 (43.9%) were appropriately prescribed antibiotics, and 4769 (34.2%) were inappropriately prescribed antibiotics. The following were independent factors of inappropriate prescription: February-March 2020 admission (OR 1.54, 95%CI 1.18–2.00), age (OR 0.98, 95%CI 0.97–0.99), absence of comorbidity (OR 1.43, 95%CI 1.05–1.94), dry cough (OR 2.51, 95%CI 1.94–3.26), fever (OR 1.33, 95%CI 1.13–1.56), dyspnea (OR 1.31, 95%CI 1.04–1.69), flu-like symptoms (OR 2.70, 95%CI 1.75–4.17), and elevated C-reactive protein levels (OR 1.01 for each mg/L increase, 95% CI 1.00–1.01). Adverse drug reactions were more frequent in patients who received ANTIBIOTIC (4.9% vs 2.7%, p < .001). Conclusion The inappropriate use of antibiotics was very frequent in COVID-19 patients and entailed an increased risk of adverse reactions. It is crucial to define criteria for their use in these patients. Knowledge of the factors associated with inappropriate prescribing can be helpful.
- Published
- 2021
15. Severe Toxoplasma gondii infection in a member of a NFKB2-deficient family with T and B cell dysfunction
- Author
-
Jacob M. Rosenberg, Marco Cappa, Annalisa Deodati, Maria Chiriaco, F. Angelini, Paul J. Utz, Andrea Angius, Silvia Di Cesare, Giovanna Stefania Colafati, S Corrente, Matteo Floris, Caterina Cancrini, Maria Elena Maccari, Alessandra Fierabracci, Paolo Rossi, Alessandro Aiuti, Alessia Scarselli, Rita Carsetti, Rosa Bacchetta, Paola Cambiaso, Maccari, Maria-Elena, Scarselli, Alessia, Di Cesare, Silvia, Floris, Matteo, Angius, Andrea, Deodati, Annalisa, Chiriaco, Maria, Cambiaso, Paola, Corrente, Stefania, Colafati, Giovanna Stefania, Utz, Paul J., Angelini, Federica, Fierabracci, Alessandra, Aiuti, Alessandro, Carsetti, Rita, Rosenberg, Jacob M., Cappa, Marco, Rossi, Paolo, Bacchetta, Rosa, and Cancrini, Caterina
- Subjects
0301 basic medicine ,B cell defect ,Immunology ,Immune-dysregulation ,NFkB2 ,STAT5A ,T cell defect ,Toxoplasmosis ,medicine.disease_cause ,Toxoplasmosi ,03 medical and health sciences ,medicine ,Immunology and Allergy ,B cell ,Settore MED/38 - Pediatria Generale e Specialistica ,biology ,business.industry ,Toxoplasma gondii ,Immune dysregulation ,biology.organism_classification ,medicine.disease ,030104 developmental biology ,medicine.anatomical_structure ,business - Published
- 2017
16. School and pre-school children with type 1 diabetes during covid-19 quarantine: the synergic effect of parental care and technology
- Author
-
Gabriele Schiaffini, Fabrizio Barbetti, Stefano Cianfarani, M. C. Matteoli, Ippolita Patrizia Patera, Annalisa Deodati, Riccardo Schiaffini, Elena Inzaghi, Novella Rapini, Paolo Ciampalini, Antonella Lorubbio, and Chiara Carducci
- Subjects
Male ,Parents ,medicine.medical_specialty ,Adolescent ,Endocrinology, Diabetes and Metabolism ,Pneumonia, Viral ,MEDLINE ,030209 endocrinology & metabolism ,Article ,03 medical and health sciences ,Betacoronavirus ,Settore MED/13 ,0302 clinical medicine ,Endocrinology ,Diabetes mellitus ,Pandemic ,medicine ,Internal Medicine ,Humans ,Hypoglycemic Agents ,Insulin ,030212 general & internal medicine ,Child ,Pandemics ,Retrospective Studies ,Type 1 diabetes ,business.industry ,SARS-CoV-2 ,Infant, Newborn ,COVID-19 ,Infant ,Retrospective cohort study ,General Medicine ,medicine.disease ,Prognosis ,Diabetes Mellitus, Type 1 ,Basal (medicine) ,Italy ,Family medicine ,Child, Preschool ,Quarantine ,Observational study ,Female ,business ,Coronavirus Infections ,Paternal care - Abstract
Introduction Management of Type 1 Diabetes (T1D) poses numerous challenges, especially for young children and their families. Parental care positively influences the outcomes of children with T1D, while there are often criticisms in school environment. The COVID-19 pandemic has forced children and parents to spend many hours at home and diabetes care has returned mainly in the hands of parents. Aim of the study To evaluate the effectiveness of exclusive return to parental care in pre-school and school children with T1D treated with Tandem Basal IQ system during the COVID-19 pandemic. Patients and methods 22 children (M:F = 14:8) with T1D have been evaluated. We compared insulin and CGM data (TIR, TBR and TAR) of two periods: PRE-COV and IN-COV, in which children have transitioned from normal school attendance to the exclusive care of their parents. Results During the IN-COV period a significantly (p < 0.001) higher median value of TIR (66,41%) was observed as compared to PRE-COV period (61,45%). Patients also showed a statistically significant difference (p < 0.002) between the IN-COV period and the PRE-COV period as concerning the TAR metric: respectively 29,86 ± 10,6 % vs 34,73 ± 12,8 %. The difference between the bolus insulin doses was statistically significant (PRE-COV 5,3 IU/day, IN-COV 7,9 IU/day – p
- Published
- 2020
17. Ectopic ACTH Secretion in a Child With Metastatic Ewing's Sarcoma: A Case Report
- Author
-
Maria Debora De Pasquale, Rita De Vito, Maria Antonietta De Ioris, Angela Mastronuzzi, Valentina Di Ruscio, Giada Del Baldo, Assunta Tornesello, Evelina Miele, Giuseppe Milano, Annalisa Deodati, and Giovanna Stefania Colafati
- Subjects
0301 basic medicine ,Cancer Research ,Pathology ,medicine.medical_specialty ,endocrine system ,Cushingoid ,Case Report ,paraneoplastic syndrome ,lcsh:RC254-282 ,03 medical and health sciences ,Cushing syndrome ,0302 clinical medicine ,medicine ,Cushing Syndrome ,hirsutism ,Acne ,business.industry ,Ewing's sarcoma ,Soft tissue ,pediatric oncology ,ACTH ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Settore MED/38 ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Sarcoma ,medicine.symptom ,business ,Weight gain ,hormones, hormone substitutes, and hormone antagonists - Abstract
Ectopic ACTH syndrome is rare in pediatric age. Sarcomas that cause Ectopic ACTH Syndrome (EAS) are even more uncommon. Currently, only three cases of EAS caused by Ewing' sarcoma have been reported. We detail a 10-year-old boy with Cushing's syndrome symptoms caused by ectopic ACTH production by a metastatic Ewing's sarcoma of the right ischio-pubic and ileo-pubic branches. The rapid appearance of cushingoid symptoms, with significant weight gain, acne, hirsutism, and hypercortisolism were implications of ectopic ACTH production as paraneoplastic Cushing's Syndrome. The very high levels of ACTH and non-suppression at the high dose dexamethasone test confirmed the clinical suspicion. We underline the possibility EAS was caused by an ACTH-secreting tumor, including soft tissue sarcomas.
- Published
- 2020
18. Biomonitoring of Bis(2-ethylhexyl)phthalate (DEHP) in Italian children and adolescents: data from LIFE PERSUADED project
- Author
-
Fabrizia Carli, Francesca Maranghi, Veronica Della Latta, Cinzia La Rocca, Amalia Gastaldelli, Stefano Cianfarani, Annalisa Deodati, Emma Buzzigoli, Roberta Tassinari, Melania Gaggini, Sabrina Tait, Enrica Fabbrizi, Luca Busani, and Giacomo Toffol
- Subjects
Male ,endocrine system ,Adolescent ,Phthalic Acids ,Total population ,010501 environmental sciences ,Age and sex ,01 natural sciences ,Biochemistry ,Reference values ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Environmental health ,Diethylhexyl Phthalate ,Biomonitoring ,Medicine ,Humans ,030212 general & internal medicine ,Child ,Preschool ,Endocrine disruptors ,0105 earth and related environmental sciences ,General Environmental Science ,Urinary volume ,Bis(2-ethylhexyl) phthalate ,business.industry ,Urinary DEHP metabolites ,Phthalate ,Children exposure ,Biological Monitoring ,Child, Preschool ,Environmental Exposure ,Female ,Italy ,Settore MED/38 ,chemistry ,Risk assessment ,business - Abstract
The Bis(2-ethylhexyl)phthalate (DEHP), a widespread plasticizer, is considered an endocrine disrupting chemical with main toxicological effects on reproductive and metabolic systems. Human biomonitoring (HBM) studies are promoted to evaluate the background exposure levels. In the frame of LIFE PERSUADED project, the HBM study measured DEHP main metabolites (mono-(2-ethylhexyl) phthalate, MEHP; 2-ethyl-5-hydroxy-hexylphthalate, MEHHP; 2-ethyl-5-oxo-hexylphthalate, MEOHP) in Italian children and adolescent (4–14 years old) according to geographical macro-areas and areas, age and sex. Children from the South and the Centre of Italy showed higher median levels of DEHP, as a sum of its metabolites (48.14 and 47.80 μg/L), than those from the North (39.47 μg/L; p = 0.0090 and 0.0004, respectively). Considering the total population, boys are more exposed than girls (only as urinary volume), and children aged 4–6 years have higher median levels than those 7–10 and 11–14 years old. The derived reference values (RV95) for DEHP in children is 168 μg/L. The relative metabolic rates of DEHP, the background levels and, thus, the RV95, vary with the geographical area, age and sex, indicating that all these parameters should be considered in the risk assessment.
- Published
- 2020
19. Epigenetics and In Utero Acquired Predisposition to Metabolic Disease
- Author
-
Stefano Cianfarani, Annalisa Deodati, and Elena Inzaghi
- Subjects
0301 basic medicine ,lcsh:QH426-470 ,Review ,Biology ,Bioinformatics ,programming ,03 medical and health sciences ,0302 clinical medicine ,microRNA ,medicine ,Genetics ,Gene silencing ,Glucose homeostasis ,Epigenetics ,Genetics (clinical) ,cardiometabolic disease ,epigenetics ,Metabolic disorder ,Lipid metabolism ,Epigenome ,intrauterine growth retardation ,medicine.disease ,Settore MED/38 ,lcsh:Genetics ,030104 developmental biology ,030220 oncology & carcinogenesis ,DNA methylation ,miRNAs ,Molecular Medicine - Abstract
Epidemiological evidence has shown an association between prenatal malnutrition and a higher risk of developing metabolic disease in adult life. An inadequate intrauterine milieu affects both growth and development, leading to a permanent programming of endocrine and metabolic functions. Programming may be due to the epigenetic modification of genes implicated in the regulation of key metabolic mechanisms, including DNA methylation, histone modifications, and microRNAs (miRNAs). The expression of miRNAs in organs that play a key role in metabolism is influenced by in utero programming, as demonstrated by both experimental and human studies. miRNAs modulate multiple pathways such as insulin signaling, immune responses, adipokine function, lipid metabolism, and food intake. Liver is one of the main target organs of programming, undergoing structural, functional, and epigenetic changes following the exposure to a suboptimal intrauterine environment. The focus of this review is to provide an overview of the effects of exposure to an adverse in utero milieu on epigenome with a focus on the molecular mechanisms involved in liver programming.
- Published
- 2020
20. Artificial Intelligence Applied to in vitro Gene Expression Testing (IVIGET) to Predict Trivalent Inactivated Influenza Vaccine Immunogenicity in HIV Infected Children
- Author
-
Alessandra Ruggiero, Sonia Zicari, Emma Concetta Manno, Paola Zangari, Nicola Cotugno, Lesley R. de Armas, Paolo Palma, Martina Fortin, Christina Bromley, Suresh Pallikkuth, Veronica Santilli, Paolo Rossi, Giuseppe Rubens Pascucci, Annalisa Deodati, Donato Amodio, Savita Pahwa, and Rajendra Pahwa
- Subjects
0301 basic medicine ,lcsh:Immunologic diseases. Allergy ,Influenza vaccine ,T cell ,Immunology ,Context (language use) ,Biology ,predictive biomarkers ,vaccinomics ,03 medical and health sciences ,0302 clinical medicine ,Immune system ,medicine ,Immunology and Allergy ,Hemagglutination assay ,Immunogenicity ,ELISPOT ,HIV ,deep learning ,artificial intelligence ,Settore MED/38 ,Vaccination ,030104 developmental biology ,medicine.anatomical_structure ,gene expression ,influenza vaccine ,lcsh:RC581-607 ,030215 immunology - Abstract
The number of patients affected by chronic diseases with special vaccination needs is burgeoning. In this scenario, predictive markers of immunogenicity, as well as signatures of immune responses are typically missing even though it would especially improve the identification of personalized immunization practices in these populations. We aimed to develop a predictive score of immunogenicity to Influenza Trivalent Inactivated Vaccination (TIV) by applying deep machine learning algorithms using transcriptional data from sort-purified lymphocyte subsets after in vitro stimulation. Peripheral blood mononuclear cells (PBMCs) collected before TIV from 23 vertically HIV infected children under ART and virally controlled were stimulated in vitro with p09/H1N1 peptides (stim) or left unstimulated (med). A multiplexed-qPCR for 96 genes was made on fixed numbers of 3 B cell subsets, 3 T cell subsets and total PBMCs. The ability to respond to TIV was assessed through hemagglutination Inhibition Assay (HIV) and ELIspot and patients were classified as Responders (R) and Non Responders (NR). A predictive modeling framework was applied to the data set in order to define genes and conditions with the higher predicted probability able to inform the final score. Twelve NR and 11 R were analyzed for gene expression differences in all subsets and 3 conditions [med, stim or Δ (stim-med)]. Differentially expressed genes between R and NR were selected and tested with the Adaptive Boosting Model to build a prediction score. The score obtained from subsets revealed the best prediction score from 46 genes from 5 different subsets and conditions. Calculating a combined score based on these 5 categories, we achieved a model accuracy of 95.6% and only one misclassified patient. These data show how a predictive bioinformatic model applied to transcriptional analysis deriving from in-vitro stimulated lymphocytes subsets may predict poor or protective vaccination immune response in vulnerable populations, such as HIV-infected individuals. Future studies on larger cohorts are needed to validate such strategy in the context of vaccination trials.
- Published
- 2020
21. A prospective case-control study on miRNA circulating levels in subjects born small for gestational age (SGA) evaluated from childhood into young adulthood
- Author
-
Mireille Vanpée, Anna Kistner, Stefano Cianfarani, Annalisa Deodati, Katarina Berinder, Elena Inzaghi, Lena Legnevall, and Daniela Germani
- Subjects
Leptin ,0301 basic medicine ,Male ,Glycated Hemoglobin A ,Physiology ,Peptide Hormones ,medicine.medical_treatment ,Type 2 diabetes ,Biochemistry ,Fats ,Endocrinology ,0302 clinical medicine ,Immune Physiology ,Medicine and Health Sciences ,Insulin ,Medicine ,Prospective Studies ,Young adult ,Prospective cohort study ,Child ,reproductive and urinary physiology ,Innate Immune System ,Multidisciplinary ,Lipids ,Settore MED/38 ,female genital diseases and pregnancy complications ,Nucleic acids ,Physiological Parameters ,Infant, Small for Gestational Age ,Cytokines ,Regression Analysis ,Female ,Adiponectin ,Research Article ,Childhood Obesity ,Adolescent ,Science ,Immunology ,030209 endocrinology & metabolism ,03 medical and health sciences ,Young Adult ,Insulin resistance ,Adipokines ,Genetics ,Adults ,Humans ,Obesity ,Circulating MicroRNA ,Non-coding RNA ,Glycated Hemoglobin ,Diabetic Endocrinology ,Natural antisense transcripts ,Biology and life sciences ,Endocrine Physiology ,business.industry ,Body Weight ,Case-control study ,Infant ,Molecular Development ,medicine.disease ,Hormones ,Gene regulation ,MicroRNAs ,030104 developmental biology ,Gene Expression Regulation ,Age Groups ,Immune System ,Case-Control Studies ,People and Places ,RNA ,Small for gestational age ,Small for Gestational Age ,Population Groupings ,Gene expression ,Insulin Resistance ,business ,Developmental Biology - Abstract
Objective microRNAs (miRNAs) associated with metabolic risk have never been extensively investigated in SGA subjects. The aim of the current study was to evaluate miRNAs in SGA and AGA subjects and their relationships with the metabolic status and growth. Design and methods A prospective longitudinal case-control study was performed in 23 SGA with postnatal catch-up growth and 27 AGA subjects evaluated at the age of 9 and 21 years. Circulating levels of miR-122-5p, miR-16-5p, miR-126-3p, and miR-486-5p were assessed by qPCR. Results SGA subjects were shorter both at 9 and at 21 years. No significant differences in insulin like growth factors and metabolic profile were found with the exception of basal glycemia at 9 years. miRNA levels did not differ between SGA and AGA subjects, at 9 and 21 years. miR-16-5p and miR-126-3p levels were higher at 9 than at 21 years. In SGA subjects, miR-122-5p at 9 years was inversely related to adiponectin levels at 21 years and miR-486-5p at 9 years was inversely related to whole-body insulin sensitivity at 9 years and directly related to Hb1Ac at 21 years. Regression analyses showed no predictive value of miRNAs for growth parameters in neither SGA nor AGA subjects. Conclusions SGA with postnatal catch-up growth did not show any difference in metabolic risk markers or miRNA circulating levels compared to AGA controls in childhood and young adulthood. miR-122-5p during childhood could identify SGA subjects at higher risk of developing insulin resistance and, eventually, type 2 diabetes in adulthood but further studies are needed to confirm it.
- Published
- 2020
22. Bone density and body composition in small for gestational age children with adequate catch up growth: A preliminary retrospective case control study
- Author
-
Danilo Fintini, Annalisa Deodati, Wolfgang Högler, Sarah Bocchini, Melania Manco, Michela Mariani, and Marco Cappa
- Subjects
Adult ,musculoskeletal diseases ,Pediatrics ,medicine.medical_specialty ,Histology ,Bone density ,Physiology ,Endocrinology, Diabetes and Metabolism ,Birth weight ,Gestational Age ,Bone Density ,medicine ,Humans ,Child ,Retrospective Studies ,Bone mineral ,Univariate analysis ,business.industry ,Case-control study ,Infant ,medicine.disease ,Body Height ,Low birth weight ,Case-Control Studies ,Body Composition ,Small for gestational age ,Female ,medicine.symptom ,business ,human activities ,Body mass index - Abstract
Background Fetal growth patterns and birth weight (BW) have been associated with bone mineral density (BMD) and content (BMC) throughout infancy and childhood up to early adulthood. We hypothesized that in small for gestational age (SGA) children, compensatory infant catch-up growth to normal height centiles counteracts the adverse consequences of low BW on bone accrual. Aim To evaluate BMD and BMC of SGA children born at term who experienced a normal catch-up growth as compared to children born appropriate for gestational age (AGA). Patients We recruited 53 SGA (26 females) and 60 AGA children (27 females), aged 6 to 18 years, matched for sex and body mass index (BMI). Fat mass (FM); Free fat mass (FFM); Lumbar spine and Total body less head (TBLH) BMD; BMC and BMD standard deviation scores corrected for body size (BMAD, BMAD z-score and TBLH BMD/Height) and TBLH BMC for FFM (TBLHBMC/FFM) were derived from Dual Energy X-ray absorptiometry (DXA) scans. Results SGA and AGA children did not differ in any auxological, body composition and bone parameters appropriately adjusted for height and FFM. BMI, FM and, remarkably, FFM were significantly correlated with bone mass parameters in both groups while no correlation was found between FM and FFM with the BW SDS in the univariate analysis. Conclusion Our preliminary data demonstrate that SGA children born at term who recover from their growth deficiency through catch-up growth achieve bone mass and body composition not different from children born AGA.
- Published
- 2021
23. Atherosclerosis as a potential pitfall in the diagnosis of giant cell arteritis
- Author
-
Luis M. Beltrán, Wolfgang A. Schmidt, Eugenio de Miguel, Juan García-Puig, Francesco Deodati, and Irene Monjo
- Subjects
Male ,medicine.medical_specialty ,Giant Cell Arteritis ,Doppler echocardiography ,Carotid Intima-Media Thickness ,Diagnosis, Differential ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Reference Values ,Internal medicine ,Humans ,Medicine ,Pharmacology (medical) ,cardiovascular diseases ,030212 general & internal medicine ,Ultrasonography, Doppler, Color ,Halo sign ,Aged ,030203 arthritis & rheumatology ,medicine.diagnostic_test ,business.industry ,Ultrasound ,Arteriosclerosis ,Middle Aged ,Atherosclerosis ,musculoskeletal system ,medicine.disease ,Temporal Arteries ,Giant cell arteritis ,Carotid Arteries ,Intima-media thickness ,cardiovascular system ,Cardiology ,Female ,medicine.symptom ,Differential diagnosis ,business ,Vasculitis ,tissues - Abstract
Objectives To explore whether the increase in the intima-media thickness (IMT) in arteriosclerotic disease correlates with the increase in the IMT in temporal arteries (TAs) and if that could mimic the US GCA halo sign. Methods Consecutive patients ⩾50 years old with high vascular risk and without signs or symptoms of GCA were included. The carotid US IMT measurements were obtained using a standardized software radiofrequency-tracking technology. Colour Doppler US and grey-scale measurements of the IMT in the branches of both TAs were performed by a second sonographer using a 22 MHz probe. Results Forty patients were studied (28 men) with a mean age of 70.6 years. The carotid IMT exhibited significant correlation with the TA IMT. A carotid IMT >0.9 mm was associated with a temporal IMT >0.3 mm. Only one patient had an IMT >0.34 mm in two branches. Conclusions Atherosclerotic disease with a carotid IMT >0.9 mm increases the TA IMT and might mimic the halo sign. As atherosclerosis is common in this age group, we propose a cut-off of TA IMT >0.34 mm in at least two branches to minimize false positives in a GCA diagnosis.
- Published
- 2017
24. Current knowledge on endocrine disrupting chemicals (EDCs) from animal biology to humans, from pregnancy to adulthood: Highlights from a national italian meeting
- Author
-
Maria E. Street, Paola Palanza, Annalisa Deodati, Giancarlo Panzica, Francesca Cirillo, Sergio Bernasconi, Anna Maria Papini, Enrica Fabbrizi, Chiara Sartori, Vassilios Fanos, Cecilia Catellani, Lorenzo Iughetti, Ernesto Burgio, Sergio Amarri, Enzo Grossi, Stefano Parmigiani, Giancarlo Gargano, Lucia Migliore, Gabriele Tridenti, Alberto Mantovani, Pietro Lazzeroni, Barbara Predieri, Sabrina Angelini, Alessandra Cassio, Street, Maria Elisabeth, Angelini, Sabrina, Bernasconi, Sergio, Burgio, Ernesto, Cassio, Alessandra, Catellani, Cecilia, Cirillo, Francesca, Deodati, Annalisa, Fabbrizi, Enrica, Fanos, Vassilio, Gargano, Giancarlo, Grossi, Enzo, Iughetti, Lorenzo, Lazzeroni, Pietro, Mantovani, Alberto, Migliore, Lucia, Palanza, Paola, Panzica, Giancarlo, Papini, Anna Maria, Parmigiani, Stefano, Predieri, Barbara, Sartori, Chiara, Tridenti, Gabriele, and Amarri, Sergio
- Subjects
0301 basic medicine ,obesity ,puberty ,Carcinogenesis ,Review ,010501 environmental sciences ,Endocrine Disruptors ,Bioinformatics ,01 natural sciences ,Endocrine Disrupting Chemicals (EDCs) ,Catalysi ,Epigenesis, Genetic ,lcsh:Chemistry ,Pregnancy ,Medicine ,lcsh:QH301-705.5 ,Carcinogenesi ,Spectroscopy ,media_common ,fertility ,neurodevelopment ,Epigenetic ,Computer Science Applications1707 Computer Vision and Pattern Recognition ,General Medicine ,Settore MED/38 ,Computer Science Applications ,Autism ,Epigenetics ,Fertility ,Growth ,Neurodevelopment ,Obesity ,Puberty ,Thyroid function ,Catalysis ,Molecular Biology ,Physical and Theoretical Chemistry ,Organic Chemistry ,Inorganic Chemistry ,Female ,Animal studies ,autism ,thyroid function ,epigenetics ,carcinogenesis ,media_common.quotation_subject ,growth ,Endocrine System ,03 medical and health sciences ,Endocrine system ,Animals ,Humans ,0105 earth and related environmental sciences ,business.industry ,medicine.disease ,030104 developmental biology ,Glucose ,lcsh:Biology (General) ,lcsh:QD1-999 ,business - Abstract
Wildlife has often presented and suggested the effects of endocrine disrupting chemicals (EDCs). Animal studies have given us an important opportunity to understand the mechanisms of action of many chemicals on the endocrine system and on neurodevelopment and behaviour, and to evaluate the effects of doses, time and duration of exposure. Although results are sometimes conflicting because of confounding factors, epidemiological studies in humans suggest effects of EDCs on prenatal growth, thyroid function, glucose metabolism and obesity, puberty, fertility, and on carcinogenesis mainly through epigenetic mechanisms. This manuscript reviews the reports of a multidisciplinary national meeting on this topic.
- Published
- 2018
25. The Importance of Apneic Events in Obstructive Sleep Apnea Associated with Acute Coronary Syndrome
- Author
-
Didier Debieuvre, Tarek El-Nazer, Didier Bresson, George Calcaianu, Beatrice Morisset, Carmen Iamandi, Daniela Holtea, Edouard Virot, Clara Deodati, and Mihaela Calcaianu
- Subjects
Acute coronary syndrome ,medicine.medical_specialty ,Article Subject ,lcsh:RC435-571 ,Cognitive Neuroscience ,Population ,Polysomnography ,030204 cardiovascular system & hematology ,03 medical and health sciences ,Behavioral Neuroscience ,0302 clinical medicine ,Internal medicine ,lcsh:Psychiatry ,Medicine ,Myocardial infarction ,Risk factor ,education ,education.field_of_study ,Ejection fraction ,medicine.diagnostic_test ,business.industry ,Apnea ,medicine.disease ,respiratory tract diseases ,Obstructive sleep apnea ,Psychiatry and Mental health ,Clinical Psychology ,030228 respiratory system ,Cardiology ,medicine.symptom ,business ,Research Article - Abstract
Background. Obstructive sleep apnea (OSA) is a potential cardiovascular risk factor. However, there is currently no prominent screening strategy for its diagnosis in patients with acute coronary syndrome (ACS). The aim of this study was to establish the impact of apneic events in case of OSA associated with ACS. Methods. Between January 1st and June 30th, fifty-three subjects with ACS (first acute myocardial infarction) were prospectively evaluated for OSA. Each patient was evaluated by polysomnography (PSG) two months after the ACS. Results. Mean age of 59±9,6 years, 81,1% males, BMI at 28,5±4,2 kg/m2, neck circumference of 42,5±12,6 cm, and waist circumference os 102,5±16,5 cm. The majority of patients (73,6%) had moderate to severe OSA (apnea-hypopnea index (AHI) ≥ 15/h and arousal index ≥ 10/h). We defined the apneic coefficient (AC) as the ratio between apnea index (AI) and AHI. We chose as cut-off the median value of apnea coefficient in our population which was at 37%. The patients with a higher AC (AC ≥ 37% versus AC < 37%) had higher levels of Troponin-I (63,4±63,2 versus 29,7±36,1 ng/mL, p=0,016), higher levels of NT-proBNP (1879,8±2141,8 versus 480±621,3 pg/mL, p=0,001), higher SYNTAX score (15,8±11,5 versus 10,2±5,9, p=0,049), and lower left ventricle ejection fraction (LVEF 53,3±11,4 versus 59,4±6,4%, p=0,023) and were more likely to have a STEMI (21 patients (77,7%) vesus 14 patients (53,8%), p=0,031). Conclusion. An apneic coefficient (AI/AHI) ≥ 37% is correlated with more severe cardiac impairment, as well as higher hypoxemia and arousal index.
- Published
- 2019
26. A screening tool for the diagnosis of severe obstructive sleep apnea in acute coronary syndrome
- Author
-
Bresson Didier, Deodati Clara, El Nazer Tarek, Calcaianu Mihaela, Calcaianu Georges-Daniel, Jacquemin Laurent, and Iamandi Carmen
- Subjects
Obstructive sleep apnea ,medicine.medical_specialty ,Acute coronary syndrome ,business.industry ,Internal medicine ,medicine ,Cardiology ,Screening tool ,medicine.disease ,business - Published
- 2018
27. The LIFE PERSUADED project approach on phthalates and bisphenol A biomonitoring in Italian mother-child pairs linking exposure and juvenile diseases
- Author
-
La Rocca, C, Maranghi, F, Tait, S, Tassinari, R, Baldi, F, Bottaro, G, Buzzigoli, E, Carli, F, Cianfarani, S, Conte, R, Deodati, A, Gastaldelli, A, Pala, Ap, Raffaelli, A, Saponaro, C, Scire, G, Spadoni, Gl, Busani, L, Narciso, L, Baldiniferroli, B, Marini, R, Germani, D, Barsotti, G, Ciociaro, D, Dellalatta, V, Distante, G, Gaggini, M, Landi, P, Di Virgilio, A, Martinelli, A, Valeri, M, Mancini, Fr, Fabbrizi, E, and Toffol, G
- Subjects
0301 basic medicine ,Male ,Pediatric Obesity ,Multivariate analysis ,Health, Toxicology and Mutagenesis ,Puberty, Precocious ,Urine ,010501 environmental sciences ,Endocrine Disruptors ,01 natural sciences ,Surveys and Questionnaires ,BPA ,Children obesity ,DEHP ,Human biomonitoring ,In vivo toxicity ,Puberty ,Adolescent ,Adult ,Animals ,Benzhydryl Compounds ,Child ,Child, Preschool ,Environmental Exposure ,Environmental Monitoring ,Environmental Pollutants ,Epidemiologic Studies ,Female ,Humans ,Italy ,Life Style ,Mothers ,Phenols ,Phthalic Acids ,Rats ,education.field_of_study ,General Medicine ,Pollution ,Settore MED/38 ,Risk assessment ,endocrine system ,Population ,03 medical and health sciences ,Environmental health ,medicine ,Environmental Chemistry ,Precocious puberty ,Endocrine system ,Juvenile ,education ,Preschool ,0105 earth and related environmental sciences ,business.industry ,medicine.disease ,Obesity ,030104 developmental biology ,Precocious ,business - Abstract
Phthalates and bisphenol A (BPA), plasticizers used in several products of daily life, are considered as endocrine disrupters, therefore children exposure is particularly relevant. The LIFE PERSUADED project aims to define the following: (a) the evaluation of internal levels of DEHP's metabolites and BPA in Italian children and their mothers, (b) the association of the exposure with puberty development and obesity diseases, and (c) the effects of exposure in juvenile in vivo model. The cross-sectional study has involved 2160 mother-child pairs, including males and females, children and adolescents, from urban and rural areas of North, Center, and South Italy. A structured questionnaire and a food diary are designed to evaluate the association between lifestyle variables potentially related to DEHP/BPA exposure and internal levels, through univariate and multivariate analyses. Two pilot case-control studies are carried out on idiopathic premature thelarche and precocious puberty (30 girls each group, aged 2-7 years) and idiopathic obesity (30 boys and 30 girls, aged 6-10 years), matched to healthy controls. BPA and DEHP's metabolites are analyzed in urine samples from all recruited subjects. Clinical and toxicological biomarkers are evaluated in serum of case-control subjects. Moreover, the toxicity study is carried out in a juvenile rodent model exposed to mixtures of BPA and DEHP at dose levels recorded in children population. The scientific results of LIFE PERSUADED will contribute to risk assessment of BPA and DEHP.
- Published
- 2018
28. Syndrome of transient headache and neurologic deficits with cerebrospinal fluid lymphocitosis should be considered in children presenting with acute confusional state
- Author
-
Laura Papetti, Irene Salfa, Riccardo Schiaffini, Federico Vigevano, Massimiliano Valeriani, Barbara Battan, Samuela Tarantino, Annalisa Deodati, and Romina Moavero
- Subjects
Male ,Weakness ,Pediatrics ,medicine.medical_specialty ,Photophobia ,Adolescent ,Nausea ,Homonymous hemianopsia ,Lymphocytosis ,03 medical and health sciences ,0302 clinical medicine ,children ,030225 pediatrics ,medicine ,Humans ,Papilledema ,Child ,Confusion ,Intracranial pressure ,Cerebrospinal Fluid ,business.industry ,HaNDL ,Headache ,altered conscious state ,intracranial hypertension ,papilledema ,Syndrome ,medicine.disease ,Settore MED/39 - Neuropsichiatria Infantile ,Neurology ,Migraine ,Vomiting ,Larva Migrans, Visceral ,Consciousness Disorders ,Female ,Neurology (clinical) ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Background: Transient headache and neurologic deficits with cerebrospinal fluid lymphocytosis (HaNDL) is benign and self-limited, with neurologic deficits including sensory disturbance of one body side, aphasia, nausea/vomiting, weakness, decreased vision, homonymous hemianopsia, photophobia. Acute confusional state can rarely occur. Papilledema and intracranial hypertension have also been described. It is a rare entity mainly affecting adults; however, it has been sporadically described in children and adolescents. Main findings: In this clinical observational study, we describe a clinical series of three consecutive pediatric patients being diagnosed with HaNDL after presenting with altered consciousness, papilledema, and increased intracranial pressure. They all recovered without relapses. Conclusion: Presentation during childhood and adolescence is rare; the majority of pediatric cases presented with altered consciousness, which is infrequent in HaNDL. This may suggest that in childhood this symptom might be more common than in adults. All three patients presented with increased intracranial pressure and papilledema, thus suggesting that these aspects should be investigated in all patients presenting with this clinical pattern. Finally, all our patients began to suffer from migraine. This feature, together with the benign course of the disease, could favor the hypothesis of a migrainous pathophysiology of this syndrome, although this remains a speculative.
- Published
- 2018
29. The exposure to uteroplacental insufficiency is associated with activation of unfolded protein response in postnatal life
- Author
-
Josepmaria Argemi, Daniela Germani, Antonella Puglianiello, Tomás J. Aragón, Cristiano De Stefanis, Valerio Nobili, Roberto Ferrero, Anna Alisi, Stefano Cianfarani, and Annalisa Deodati
- Subjects
0301 basic medicine ,Leptin ,Male ,X-Box Binding Protein 1 ,Adult life ,Messenger ,Unfolded protein response (UPR) ,lcsh:Medicine ,Fatty Acids, Nonesterified ,Endoplasmic Reticulum ,Rats, Sprague-Dawley ,Pregnancy ,lcsh:Science ,Endoplasmic Reticulum Chaperone BiP ,Heat-Shock Proteins ,Glucose tolerance test ,Multidisciplinary ,Fetal Growth Retardation ,medicine.diagnostic_test ,Fatty Acids ,Aspartate-Ammonia Ligase ,Settore MED/38 ,Liver ,Lipogenesis ,Metabolome ,Female ,medicine.medical_specialty ,Biology ,03 medical and health sciences ,Downregulation and upregulation ,Internal medicine ,medicine ,Animals ,RNA, Messenger ,Disease Models, Animal ,Gene Expression Regulation ,Glucose Tolerance Test ,Rats ,Unfolded Protein Response ,Animal ,Endoplasmic reticulum ,lcsh:R ,medicine.disease ,030104 developmental biology ,Endocrinology ,Gluconeogenesis ,Chronic diseases ,Disease Models ,Nonesterified ,Unfolded protein response ,RNA ,lcsh:Q ,Sprague-Dawley ,Steatosis ,Homeostasis - Abstract
Early life events are associated with the susceptibility to chronic diseases in adult life. Perturbations of endoplasmic reticulum (ER) homeostasis activate the unfolded protein response (UPR), which contributes to the development of metabolic alterations. Our aim was to evaluate liver UPR in an animal model of intrauterine growth restriction (IUGR). A significantly increased expression of X-box binding protein-1 spliced (XBP1s) mRNA (p
- Published
- 2018
30. SAT0609 Giant cell arteritis: atherosclerosis as pitfall in the halo sign
- Author
-
E. De Miguel, Juan García-Puig, Luis M. Beltrán, Irene Monjo, F Deodati, and Wolfgang A. Schmidt
- Subjects
medicine.diagnostic_test ,business.industry ,medicine.disease ,Grey scale ,Giant cell arteritis ,medicine.anatomical_structure ,Erythrocyte sedimentation rate ,cardiovascular system ,medicine ,Cutoff ,Disease prevention ,cardiovascular diseases ,Halo ,medicine.symptom ,business ,Nuclear medicine ,Halo sign ,Artery - Abstract
Background The halo sign (defined as an homogenous, hypoechoic wall thickening, well delineated towards the luminal side, visible in longitudinal and transverse planes). is the more accurate ultrasonographic (US) finding for the diagnosis of Giant Cell Arteritis (GCA). False-positive halos are seen in malignant and infectious diseases. The new US high frequencies probes make possible not only to see the halo sign but also measure the increase of the intima-media-thickness (IMT). Moreover, the US appearance of the IMT in atherosclerotic carotid artery may be hypoechoic as the halo sign. Objectives The aim of this study was to explore if the increase of the atherosclerotic IMT can mimic the halo sign in temporal arteries (TA) and to produce false positives in the diagnosis of GCA. Methods Consecutive non selected patients, ≥50 years-old with high vascular risk according to European Guidelines on Cardiovascular Disease Prevention, and without signs or symptoms of GCA, were included. Ultrasonography of carotid artery: Carotid ultrasound examinations were performed on a Mylab Seven (Esaote Medical Systems, Italy) with a 4–13 MHz linear-array. The system employed dedicated software radiofrequency-tracking technology to obtain IMT (QIMT®). Ultrasonography of temporal superficial artery: A color Doppler ultrasound (CDU) and grey scale measure of the IMT/halo sign in the branches of both TA was performed by a second experienced sonographer. A Mylab Twice equipment (Esaote, Geneve, Italy) was used, with a 22 MHz frequency for grey scale and a 12.5 MHz for CDU, with a color gain of 51 and a PRF of 2 kHz. The sonographer was blind to the clinical data and carotid ultrasound IMT measures. Examination videos were stored for reliability and an intra-reader was performed months after the examination. Statistical analysis: Descriptive frequencies, Student9s t -test, Cronbach9s alpha and Spearman correlations was used. Results Forty patients were studied, 28 men (70%), with a mean age of 70,6±6,9 years. Three patients were active smoker and 27 ex-smokers. Arterial hypertension was present in 39 (97.5%), dyslipidemia in 34 (85%) and diabetes in 19 (47.5%). The mean erythrocyte sedimentation rate was 13.6±11.0. Eighty carotids were studied, 50 had plaques and 30 did not with a IMT ranged from 0.528 to 1.480 mm. The mean values of TA related with the carotid IMT are presented in the table: the increase in the carotid IMT is associated with an increase in the IMT of the TA with a weak Spearman correlation (parietal branches 0.282 p=0.012 and frontal branches 0.228 p=0.048). From reliability a Cronbach9s alpha of 0.900 and 0.876 were achieved for parietal and frontal branches respectively. Some patients had a TA IMT that can be interpreted as halo sign: 18 (45%) patients and 33 (20.6%) TA branches if we choose a cutoff >0.30 mm of IMT/halo sign; 4 (10%) patients and 7 (4.4%) TA branches if the cutoff were >0.34 mm. Conclusions The atherosclerotic disease can produce false-positive GCA diagnosis. Carotid IMT >0.9 mm is associated with halo sign in TA. The IMT cutoff value for the diagnosis of GCA should be established. Disclosure of Interest None declared
- Published
- 2017
31. AB1024 Ultrasound in giant cell arteritis: cut-off and pitfalls in the halo sign
- Author
-
E. De Miguel, Juan García-Puig, Wolfgang A. Schmidt, F Deodati, Luis M. Beltrán, and Irene Monjo
- Subjects
medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Ultrasound ,medicine.disease ,Giant cell arteritis ,medicine.anatomical_structure ,Erythrocyte sedimentation rate ,Sonographer ,cardiovascular system ,Medicine ,cardiovascular diseases ,Cut-off ,Halo ,Radiology ,medicine.symptom ,business ,Halo sign ,Artery - Abstract
Background At the age of presentation of Giant Cell Arteritis (GCA) atherosclerosis is common. The ultrasonographic (US) appearance of athermanous plaque is usually easily differentiated from the hypoechoic halo of GCA. However, the US appearance of the increased of the intima-media-thickness (IMT) in an atherosclerotic carotid artery may have similar image appearance as the halo sign. We hypothesize that atherosclerosis could produce an increase of temporal artery (TA) IMT and cause false-positives halo sign. Objectives The aim of this study was to explore the better cut-off in the IMT of TA to minimise the number of false-positive GCA diagnosis caused by atherosclerosis. Methods Consecutive non selected patients, ≥50 years-old with high vascular risk according to European Guidelines on cardiovascular disease prevention, and without signs or symptoms of GCA, were included. Ultrasonography of carotid artery: Carotid US examinations were performed on a Mylab Seven (Esaote Medical Systems, Italy) with a 4–13 MHz linear-array. The system employed dedicated software radiofrequency-tracking technology to obtain IMT (QIMT®). Ultrasonography of temporal superficial artery: A color Doppler ultrasound (CDU) and grey scale measure of the IMT/halo sign in both TA and its branches was performed by a second experienced sonographer. A Mylab Twice equipment (Esaote, Geneve, Italy) was used, with a 22 MHz frequency for grey scale and a 12.5 MHz for CDU (color gain of 51, PRF of 2 kHz). The sonographer was blind to the clinical and carotid ultrasound IMT data. Results Forty patients were studied, 28 men (70%), with a mean age of 70,6±6,9 years. Three patients were active smokers and 27 ex-smokers. Arterial hypertension was present in 39 (97.5%), dyslipidaemia in 34 (85%) and diabetes in 19 (47.5%). The mean erythrocyte sedimentation rate was 13.6±11.0. The table shows that an IMT >0.30 mm (halo sign) was seen in at least 1 TA branch of 18 patients (45%) with 33 TA branches affected (20.6%). An IMT cut-off >0.34 mm, was present in 4 patients (10%). When at least two affected branches with this measure were required to make the US diagnosis (criteria recommended to improve specificity) only one patient (2.5%) produced a false-positive halo sign. Conclusions To the best of our knowledge, this is the first communication indicating that atherosclerosis is a potential cause of false-positive halo sign. We propose a cut-off of AT IMT >0.34 mm in at least two branches to minimise the number of false positives in GCA diagnosis. Disclosure of Interest None declared
- Published
- 2017
32. The Rationale for Growth Hormone Therapy in Children with Short Stature
- Author
-
Stefano Cianfarani and Annalisa Deodati
- Subjects
Male ,Endocrinology, Diabetes and Metabolism ,Physiology ,030209 endocrinology & metabolism ,Review ,Growth hormone ,Short stature ,03 medical and health sciences ,Strength of evidence ,0302 clinical medicine ,Endocrinology ,idiopathic short stature ,idiopathic short stature small for gestational age ,medicine ,Growth hormone treatment ,Humans ,Child ,Growth Disorders ,business.industry ,Human Growth Hormone ,Infant ,medicine.disease ,Settore MED/38 ,Body Height ,Idiopathic short stature ,030220 oncology & carcinogenesis ,Pediatrics, Perinatology and Child Health ,Infant, Small for Gestational Age ,Small for gestational age ,Small for Gestational Age ,Female ,medicine.symptom ,business ,GH Deficiency ,Hormone - Abstract
Growth hormone (GH) was first isolated from cadaver pituitary glands, requiring laborious and expensive collection of glands, followed by extraction and purification of the hormone. This limited supply restricted its use to children with severe GH deficiency who were treated with low dosages and suboptimal schedules. The development of recombinant DNA-derived GH, allowed the production of virtually unlimited amounts of GH, leading to the approval for therapy for a large number of childhood conditions characterized by non-GH deficient short stature. The aim of this review is to provide a critical overview on the daily use of GH in two paradigmatic conditions of non-GH deficient short stature which are children born small for gestational age and with idiopathic short stature, highlighting the available strength of evidence for efficacy and safety.
- Published
- 2017
33. IGF2 Methylation Is Associated with Lipid Profile in Obese Children
- Author
-
Claudia Brufani, Antonella Puglianiello, Daniela Germani, Danilo Fintini, Alice Liguori, Marco Cappa, Elena Inzaghi, Stefano Cianfarani, Annalisa Deodati, and Fabrizio Barbetti
- Subjects
Genetics ,medicine.medical_specialty ,medicine.diagnostic_test ,Endocrinology, Diabetes and Metabolism ,Methylation ,Biology ,medicine.disease ,Obesity ,Endocrinology ,CpG site ,Internal medicine ,Pediatrics, Perinatology and Child Health ,DNA methylation ,medicine ,Population study ,Epigenetics ,Lipid profile ,Dyslipidemia - Abstract
Aim: Our aim was to investigate the relationships between the degree of IGF2 methylation and the metabolic status in obese children and adolescents. Subjects and Methods: Eighty-five obese subjects aged 11.6 ± 2.1 years were studied. Anthropometry, metabolic parameters, blood pressure and body composition were assessed. DNA methylation analysis was performed by restriction enzyme digestion assay. The study population was subdivided into two groups according to the percentage of IGF2 cytidine-guanosine (CpG) island methylation. Results: Twenty-two subjects showed intermediate methylation (a percentage of CpG site methylation comprised between 10 and 60%), 56 were hypomethylated (percentage of methylation lower than 10%), and only 1 showed a high rate of hypermethylation (percentage of methylation above 60%). Children with intermediate methylation showed significantly higher levels of triglycerides (107.6 ± 41.99 vs. 76.6 ± 30.18 mg/dl, p < 0.005) and a higher triglyceride/high-density lipoprotein-cholesterol ratio (2.23 ± 0.98 vs. 1.79 ± 0.98, p < 0.02) compared with hypomethylated children. Conclusions: These preliminary findings show for the first time a relationship between IGF2 methylation pattern and lipid profile in obese children. Although the correlation does not imply causation, if our findings are confirmed in further studies, IGF2 methylation might represent an epigenetic marker of metabolic risk.
- Published
- 2013
34. Age exacerbates sickness behavior following exposure to a viral mimetic
- Author
-
Marielle S. Kahn, Gary W. Boehm, Kristina A. McLinden, Lauren E. Deodati, Dinko Kranjac, and Michael J. Chumley
- Subjects
Male ,Aging ,medicine.medical_specialty ,Hippocampus ,Posterior parietal cortex ,Experimental and Cognitive Psychology ,Mice ,Behavioral Neuroscience ,Biomimetic Materials ,Parietal Lobe ,Internal medicine ,medicine ,Animals ,Inflammatory factors ,Sickness behavior ,Illness Behavior ,Innate immune system ,Behavior, Animal ,Interleukins ,Peripheral ,Cortex (botany) ,Mice, Inbred C57BL ,Poly I-C ,Endocrinology ,Immunology ,Cytokines ,Chemokines ,Psychology ,Immune activation - Abstract
Poly I:C, a viral mimetic, is a synthetic double-stranded RNA that is known to cause activation of the innate immune system, resulting in the emergence of sickness behaviors in otherwise healthy adult mice. However, the way in which such effects of poly I:C manifest themselves in aged mice are not currently known. We hypothesized that poly I:C administration would lead to burrowing deficits, but that these deficits would be exaggerated in aged subjects (19-months old) compared to young subjects (4-months old) that received the same dose. In order to associate these behavioral decrements with inflammatory factors, we measured mRNA expression of IL-1β and IL-6 in the hippocampus and parietal cortex and peripheral protein expression of IL-6, TNF-α, MCP-1, MIP-1α, and IL-1β in the serum. After exposure to poly I:C, aged subjects demonstrated significant impairments in their burrowing behavior, compared to younger subjects administered the same dose. These behavioral decrements coincided with increased expression of IL-6 among animals exposed to poly I:C and increased expression of IL-1β among aged animals in the hippocampus and cortex. Furthermore, we observed an increase in peripheral poly I:C-induced IL-6, TNF-α, MCP-1, and MIP-1α, but not IL-1β. These results indicate that virus-mediated immune activation in the aging body can lead to increased sickness behavior. Furthermore, these data indicated a possible dissociation between the effects of poly I:C on sickness behaviors in aged mice, with central expression of IL-1β potentially playing a role in age-related impairments.
- Published
- 2012
35. Peripheral bacterial endotoxin administration triggers both memory consolidation and reconsolidation deficits in mice
- Author
-
Gary W. Boehm, Lauren E. Deodati, Mauricio R. Papini, Dinko Kranjac, Kristina A. McLinden, and Michael J. Chumley
- Subjects
Lipopolysaccharides ,Male ,medicine.medical_treatment ,Interleukin-1beta ,Immunology ,Central nervous system ,Hippocampus ,Context (language use) ,Real-Time Polymerase Chain Reaction ,Proinflammatory cytokine ,Mice ,Behavioral Neuroscience ,Discrimination, Psychological ,Neurotrophic factors ,Weight Loss ,medicine ,Animals ,Learning ,Early Growth Response Protein 1 ,Brain Chemistry ,Cerebral Cortex ,Memory Disorders ,Behavior, Animal ,Endocrine and Autonomic Systems ,Brain-Derived Neurotrophic Factor ,Fear ,Impaired memory ,Endotoxins ,Mice, Inbred C57BL ,Cytokine ,medicine.anatomical_structure ,Conditioning, Operant ,Cytokines ,Memory consolidation ,Chemokines ,Psychology ,Neuroscience - Abstract
Peripherally administered inflammatory stimuli, such as lipopolysaccharide (LPS), induce the synthesis and release of proinflammatory cytokines and chemokines in the periphery and the central nervous system, and trigger a variety of neurobiological responses. Indeed, prior reports indicate that peripheral LPS administration in rats disrupts contextual fear memory consolidation processes, potentially due to elevated cytokine expression. We used a similar, but partially olfaction-based, contextual fear conditioning paradigm to examine the effects of LPS on memory consolidation and reconsolidation in mice. Additionally, interleukin-1β (IL-1β), brain-derived neurotrophic factor (BDNF), and zinc finger (Zif)-268 mRNA expression in the hippocampus and the cortex, along with peripheral cytokines and chemokines, were assessed. As hypothesized, LPS administered immediately or 2 h, but not 12 h, post-training impaired memory consolidation processes that support the storage of the conditioned contextual fear memory. Additionally, as hypothesized, LPS administered immediately following the fear memory trace reactivation session impaired memory reconsolidation processes. Four hours post-injection, both central cytokine and peripheral cytokine and chemokine levels were heightened in LPS-treated animals, with a simultaneous decrease in BDNF, but not Zif-268, mRNA. Collectively, these data reinforce prior work showing LPS- and cytokine-related effects on memory consolidation, and extend this work to memory reconsolidation.
- Published
- 2012
36. Serum Levels of Polybrominated Diphenyl Ethers in Girls with Premature Thelarche
- Author
-
Roberta Tassinari, Francesca Mancini, Alberto Mantovani, Stefano Cianfarani, Annalisa Deodati, Daniela Germani, Alessia Sallemi, Francesca Maranghi, Luca Busani, Antonella Puglianiello, and Francesca Baldari
- Subjects
0301 basic medicine ,endocrine system ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,Puberty, Precocious ,010501 environmental sciences ,01 natural sciences ,Settore MED/13 - Endocrinologia ,03 medical and health sciences ,fluids and secretions ,Endocrinology ,Polybrominated diphenyl ethers ,Premature thelarche ,Internal medicine ,medicine ,Halogenated Diphenyl Ethers ,Precocious puberty ,Endocrine system ,Humans ,Breast ,Child ,Idiopathic central precocious puberty ,reproductive and urinary physiology ,0105 earth and related environmental sciences ,Settore MED/38 - Pediatria Generale e Specialistica ,business.industry ,medicine.disease ,humanities ,030104 developmental biology ,Cross-Sectional Studies ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Female ,business - Abstract
Background/Aims: Polybrominated diphenyl ethers (PBDEs) are widely used as flame retardants and have shown endocrine disruption properties in experimental studies. The aim of this study was to investigate the association between the exposure to PBDEs and alterations of puberty in girls referred for idiopathic central precocious puberty (ICPP) and premature thelarche (PT). Methods: A case-control study was conducted in 124 girls: 37 girls with ICPP (mean age 7.4 ± 0.9 years), 56 with PT (mean age 5.7 ± 2.1 years) and 31 controls (mean age 5.4 ± 1.9 years). PBDE serum concentrations, hormone levels and anthropometry were assessed. PBDE concentrations were corrected for total serum lipid content. Individual exposure to PBDEs was evaluated through ad hoc questionnaires. Results: PBDE serum concentrations corrected for total lipid content were significantly higher in girls with PT (mean 1.49 ± 0.63 log ng/g) than in controls (mean 1.23 ± 0.54 log ng/g; p < 0.05). PT girls showed higher levels of PBDE than ICPP girls (1.49 ± 0.63 vs. 1.37 ± 0.49 log ng/g), though this was not significant. An analysis of the questionnaires revealed no significant differences in exposure between the three groups. Conclusion: Our findings suggest that higher concentrations of serum PBDEs are associated with PT in girls.
- Published
- 2015
37. Manifestations ophtalmologiques d�un lupus �ryth�mateux chronique � propos d�une observation
- Author
-
F. Deodati, G. Bechac, P. Bec, and A. Bazex
- Subjects
medicine.diagnostic_test ,business.industry ,Medicine ,business ,Fluorescein angiography ,Nuclear medicine - Published
- 2015
38. Review of Growth Hormone Randomized Controlled Trials in Children with Idiopathic Short Stature
- Author
-
Stefano Cianfarani, Annalisa Deodati, and Emanuela Peschiaroli
- Subjects
Adult ,Pediatrics ,medicine.medical_specialty ,Adolescent ,Response to therapy ,Endocrinology, Diabetes and Metabolism ,MEDLINE ,Growth hormone ,law.invention ,Endocrinology ,Randomized controlled trial ,law ,Humans ,Medicine ,Preschool ,Child ,Growth Disorders ,Randomized Controlled Trials as Topic ,Settore MED/38 - Pediatria Generale e Specialistica ,Human Growth Hormone ,business.industry ,Human growth hormone ,Mean age ,Body Height ,Recombinant Proteins ,Child, Preschool ,medicine.disease ,Adult height ,Idiopathic short stature ,Pediatrics, Perinatology and Child Health ,business - Abstract
Recombinant human growth hormone (GH) has been used to increase growth and adult height in children with idiopathic short stature (ISS) despite the lack of definitive, long-term studies of efficacy. Our aim was to systematically review all randomized controlled trials (RCTs) of GH use in children with ISS.We performeda formal search of the Cochrane Central Register of Controlled Trials, Medline and the bibliographic references from all retrieved articles describing such trials up to August 2009. Three long-term RCTs conducted to the achievement of adult height were identified. The mean age at start of treatment in the three trials was 10.1 ± 3.4 years. The mean duration of GH therapy was 5.4 ± 0.9 years. The mean difference between treated and untreated children was 0.77 ± 0.4 standard deviation score (SDS) (approximately 5.5 cm). The mean adult height achievedafter GH therapy at conventional doses was –1.5 ± 0.3 SDS. GH improves adult height in children with ISS. However, treated individuals remain relatively short compared with peers of normal height. The individual response to therapy is highly variable and further studies are required to identify responders. Lastly, high-quality evidence from long-term RCTs of GH therapy that continue until achievement of adult height is necessary to determine the ideal dosage and long-term safety.
- Published
- 2011
39. The impact of growth hormone therapy on adult height in noonan syndrome: a systematic review
- Author
-
Mohamad G. Shaikh, Stefano Cianfarani, Syed Faisal Ahmed, Annalisa Deodati, and Claudio Giacomozzi
- Subjects
Isi web of science ,Adult ,Pediatrics ,medicine.medical_specialty ,Adolescent ,Hormone Replacement Therapy ,Endocrinology, Diabetes and Metabolism ,MEDLINE ,Growth hormone ,Endocrinology ,medicine ,Humans ,Child ,Settore MED/38 - Pediatria Generale e Specialistica ,business.industry ,Human Growth Hormone ,Noonan Syndrome ,Infant ,medicine.disease ,Adult height ,Body Height ,Clinical trial ,Growth hormone treatment ,Child, Preschool ,Pediatrics, Perinatology and Child Health ,Noonan syndrome ,business ,Linear growth - Abstract
Background: Recombinant human growth hormone (rhGH) is being used to promote linear growth in short children with Noonan syndrome. However, its efficacy is still controversial. Aims: To systematically determine the impact of rhGH therapy on adult height in children with Noonan syndrome. Methods: We searched the Cochrane Central Register of Controlled Trials, ISI Web of Science, MEDLINE, and the bibliographic references from all retrieved articles published until April 2014. Studies reporting adult/near-adult height in children with Noonan syndrome treated with rhGH or reporting at least a 3-year follow-up were analysed. Quality and strength of recommendation were assessed according to the Endocrine Society criteria. Results: No controlled trials reporting adult height were available. Five studies were identified reporting adult height or near adult height. Data comparison showed inter-individual variability in the response to rhGH, mean height gain standard deviation score ranging between 0.6 and 1.4 according to national standards, and between 0.6 and 2 according to Noonan standards. Significant biases affected all the studies. Conclusions: High-quality controlled trials on the impact of rhGH therapy on adult height are lacking, and the robustness of available data is not sufficient to recommend such therapy in children with Noonan syndrome.
- Published
- 2014
40. Association between growth hormone therapy and mortality, cancer and cardiovascular risk: systematic review and meta-analysis
- Author
-
Barbara Baldini Ferroli, Stefano Cianfarani, and Annalisa Deodati
- Subjects
Oncology ,medicine.medical_specialty ,Adolescent ,Endocrinology, Diabetes and Metabolism ,Growth hormone therapy ,Dwarfism ,Growth hormone ,Settore MED/13 - Endocrinologia ,Endocrinology ,Risk Factors ,Neoplasms ,Internal medicine ,medicine ,Humans ,In patient ,Cardiovascular mortality ,Settore MED/38 - Pediatria Generale e Specialistica ,business.industry ,Confounding ,Cancer ,Metaanalysis ,medicine.disease ,Surgery ,IGF-I ,Standardized mortality ratio ,Cardiovascular Diseases ,Child, Preschool ,Meta-analysis ,Relative risk ,business - Abstract
The potential involvement of growth hormone therapy in tumor promotion and progression has been of concern for several decades. Our aim was to assess systematically the association between growth hormone therapy and all-cause, cancer and cardiovascular mortality, cancer morbidity and risk of second neoplasm mainly in patients treated during childhood and adolescence.A systematic review of all articles published until September 2013 was carried out. The primary efficacy outcome measures were the all-cause, cancer and cardiovascular standardized mortality ratios (SMR). The secondary efficacy outcome measures were the standardized incidence ratio (SIR) for cancer and the relative risk (RR) for second neoplasms. The global effect size was calculated by pooling the data. When the effect size was significant in a fixed model we repeated the analyses using a random model.The overall all-cause SMR was 1.19 (95% CI 1.08-1.32, p0.001). Malignancy and cardiovascular SMRs were not significantly increased. Both the overall cancer SIR 2.74 (95% CI 1.18-5.41), and RR for second neoplasms 1.99 (95% CI 1.28-3.08, p=0.002), were significantly increased.The results of this meta-analysis may raise concern on the long-term safety of GH treatment. However, several confounders and biases may affect the analysis. Independent, long-term, well-designed studies are needed to properly address the issue of GH therapy safety.
- Published
- 2014
41. Cardiovascular fitness is impaired in children born small for gestational age
- Author
-
Attilio Turchetta, Marco Cappa, Rossana Fiori, Danilo Fintini, Giulia Cafiero, Stefano Cianfarani, Annalisa Deodati, Claudia Brufani, and Ugo Giordano
- Subjects
Settore MED/38 - Pediatria Generale e Specialistica ,Male ,Pediatrics ,medicine.medical_specialty ,business.industry ,Infant, Newborn ,General Medicine ,medicine.disease ,Settore MED/13 - Endocrinologia ,Physical Fitness ,Case-Control Studies ,Pediatrics, Perinatology and Child Health ,Infant, Small for Gestational Age ,medicine ,Exercise Test ,Small for gestational age ,Humans ,Female ,Prospective Studies ,business ,Child ,Cardiovascular fitness - Published
- 2013
42. Anesthetic Volume for Ultrasound-Guided 'Double Bubble' Infraclavicular Block: Comparison of Ropivacaine 0.75% 30ml Vs 35ml
- Author
-
Alessandro Di Filippo, Alberto Boccaccini, Maria Consolata Campolo, Christian Maria Visco, Rossella Deodati, Angelo Raffaele De Gaudio, Amer Georges Iskandar, and Adele Molinaro
- Subjects
medicine.medical_specialty ,Ropivacaine ,business.industry ,medicine.disease ,Ultrasound guided ,Double bubble ,Surgery ,law.invention ,Anesthesiology and Pain Medicine ,medicine.anatomical_structure ,Randomized controlled trial ,law ,Anesthesia ,Anesthetic ,medicine ,Coagulopathy ,Infusion pump ,business ,Anesthetics ,Dose response relationship ,Drug ,Local ,Peripheral nerves ,Ultrasonography ,medicine.drug ,Artery - Abstract
For an UltraSound guided Infraclavicular Block, a non-inferiority randomized study was conducted comparing two volumes of ropivacaine 0.75%: 35 ml vs 30ml. Fifty 18-70 years old patients undergoing upper limb surgery, ASA I-II were enrolled. Exclusion criteria included existing neurologic disease, coagulopathy, allergy, pregnancy, previous surgery in clavicular region, BMI more than 30 kg/m2 or patients unable to give written consent. Using US guidance, a 22 gauge/80 mm SonoPlex needle (Pajunk®) was advanced until the tip was located dorsally to the artery at a 6-o’clock position. Correct placement was ensuring by a “double bubble” sign. The block was performed by delivering ropivacaine 0.75% via an infusion pump (Alaris® PK) at 600 ml/h.
- Published
- 2012
43. Impact of growth hormone therapy on adult height of children with idiopathic short stature: systematic review
- Author
-
Stefano Cianfarani and Annalisa Deodati
- Subjects
Adult ,Male ,Pediatrics ,medicine.medical_specialty ,Dwarfism ,Randomized Controlled Trials as Topic ,Human Growth Hormone ,Body Height ,Humans ,Treatment Outcome ,Child ,Adolescent ,Female ,Response to therapy ,Treatment outcome ,Growth hormone ,Short stature ,Medicine ,General Environmental Science ,Settore MED/38 - Pediatria Generale e Specialistica ,Treated group ,business.industry ,General Engineering ,General Medicine ,medicine.disease ,Settore MED/38 ,Adult height ,Idiopathic short stature ,Prepubertal stage ,General Earth and Planetary Sciences ,medicine.symptom ,business - Abstract
Objective To systematically determine the impact of growth hormone therapy on adult height of children with idiopathic short stature. Design Systematic review. Data sources Cochrane Central Register of Controlled Trials, Medline, and the bibliographic references from retrieved articles of randomised and non-randomised controlled trials from 1985 to April 2010. Data extraction Height in adulthood (standard deviation score) and overall gain in height (SD score) from baseline measurement in childhood. Study selection Randomised and non-randomised controlled trials with height measurements for adults. Inclusion criteria were initial short stature (defined as height >2 SD score below the mean), peak growth hormone responses >10 μg/L, prepubertal stage, no previous growth hormone therapy, and no comorbid conditions that would impair growth. Adult height was considered achieved when growth rate was Results Three randomised controlled trials (115 children) met the inclusion criteria. The adult height of the growth hormone treated children exceeded that of the controls by 0.65 SD score (about 4 cm). The mean height gain in treated children was 1.2 SD score compared with 0.34 SD score in untreated children. A slight difference of about 1.2 cm in adult height was observed between the two growth hormone dose regimens. In the seven non-randomised controlled trials the adult height of the growth hormone treated group exceeded that of the controls by 0.45 SD score (about 3 cm). Conclusions Growth hormone therapy in children with idiopathic short stature seems to be effective in partially reducing the deficit in height as adults, although the magnitude of effectiveness is on average less than that achieved in other conditions for which growth hormone is licensed. The individual response to therapy is highly variable, and additional studies are needed to identify the responders.
- Published
- 2011
44. PP216-SUN: Nutrition Evaluation at Hospital Admission in Internal Medicine
- Author
-
J.A. García García, F. Deodati, F.J. Flores Alvarez, M. González Suárez, and E. Jimenez Rodriguez
- Subjects
medicine.medical_specialty ,Nutrition and Dietetics ,business.industry ,Internal medicine ,Emergency medicine ,Hospital admission ,medicine ,Critical Care and Intensive Care Medicine ,business - Published
- 2014
45. Epigenetic changes predisposing to type 2 diabetes in intrauterine growth retardation
- Author
-
Alice Liguori, Antonella Puglianiello, Emanuela Peschiaroli, Stefano Cianfarani, Annalisa Deodati, and Daniela Germani
- Subjects
Settore MED/38 - Pediatria Generale e Specialistica ,lcsh:RC648-665 ,epigenetics ,diabetes ,Growth retardation ,business.industry ,Endocrinology, Diabetes and Metabolism ,Review Article ,Type 2 diabetes ,Epigenome ,Intrauterine growth retardation ,medicine.disease ,Bioinformatics ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Endocrinology ,Diabetes mellitus ,medicine ,Endocrine system ,Epigenetics ,business ,Stroke ,Organism - Abstract
Epidemiologic studies have demonstrated an association between intrauterine growth retardation and a greater risk of chronic disease, including coronary heart disease, hypertension, stroke, and type 2 diabetes in adulthood. An adverse intrauterine environment may affect both growth and development of the organism, permanently programming endocrine and metabolic functions. One of the mechanisms of programming is the epigenetic modification of gene promoters involved in the control of key metabolic pathways. The aim of this review is to provide an overview of the experimental evidence showing the effects of early exposure to suboptimal environment on epigenome. The knowledge of the epigenetic markers of programming may allow the identification of susceptible individuals and the design of targeted prevention strategies.
- Published
- 2010
46. Three-dimensional reconstruction of the spatial distribution of anaesthetic during locoregional anaesthesia
- Author
-
L. Bocchi, L. Larucci, R. Giabbiani, R. Deodati, P. Ruggiano, S. Orlandi, P. Ruggiano, L. Larucci, R. Deodati, R. Giabbiani, L. Bocchi, and S. Orlandi
- Subjects
Locoregional anaesthesia ,medicine.medical_specialty ,Anesthesiology and Pain Medicine ,business.industry ,Ultrasound guided anaesthesia ,anesthetic distribution ,medicine ,Radiology ,Three-dimensional reconstruction ,business - Published
- 2013
47. Subject Index Vol. 79, 2013
- Author
-
Ari J. Wassner, Sonir Roberto Rauber Antonini, Thomas Meissner, Jan de Laffolie, Claudia Boettcher, Reinhard W. Holl, Michaela F. Hartmann, Fernando Carrasco, Kang Min Wu, Peter Beyer, Ulrich Brand, Antonella Puglianiello, Jörg Fussenegger, Carlos Eduardo Martinelli, Verónica Mericq, Shira Oren, Laurie E. Cohen, Stefan A. Wudy, C.F. Munns, Dan Nemet, Rodrigo José Custódio, Druck Reinhardt Druck Basel, Eliana Hechter, Débora Macedo Cabral, Alice Liguori, Berthold P. Hauffa, Daniela Germani, Andreas Hungele, Carolina Sepúlveda, Stefano Cianfarani, Enrica Pittaluga, Annalisa Deodati, Michal Pantanowitz, Claudia Brufani, Alejandra Avila, Carlos Antonio Bruno da Silva, Holger Haberland, C. Bridge, M.B. Alcausin, Satz Mengensatzproduktion, J. Ault, Alon Eliakim, Fabrizio Barbetti, Andrew Dauber, Burak Salgin, Martin Borkenstein, J. Briody, D.O. Sillence, Cinthya Urquidi, R.H.H. Engelbert, Elena Inzaghi, Chuan Shen, Danilo Fintini, Klaus-Peter Zimmer, Marco Cappa, V. Pacey, Zhang Hui Tang, M. McQuade, Germán Iñiguez, and Tao Yu
- Subjects
Gerontology ,Endocrinology ,Index (economics) ,business.industry ,Endocrinology, Diabetes and Metabolism ,Pediatrics, Perinatology and Child Health ,Physiology ,Medicine ,Subject (documents) ,business - Published
- 2013
48. Subject Index Vol. 76, Suppl. 3, 2011
- Author
-
Satz Mengensatzproduktion, Jan M. Wit, Druck Reinhardt Druck Basel, Tassilo Kruis, John Chaplin, Marina Schlicke, Christopher J. H. Kelnar, Roland Pfäffle, Emanuela Peschiaroli, Stefano Cianfarani, Annalisa Deodati, Sandro Loche, Paul Czernichow, Wieland Kiess, Jürgen Klammt, Peter Fibiger Bang, Isabelle Durand-Zaleski, Meinolf Noeker, Leo Dunkel, Anders Lindberg, L.B. Johnston Rohrbasser, Tillmann Wallborn, Rasha Odeh, Anita C. S. Hokken-Koelega, Markus Perola, E. E. Müller, Jovanna Dahlgren, Monika Bullinger, Michael B. Ranke, Juergen Kratzsch, David B. Allen, David E. Sandberg, and Steven D. Chernausek
- Subjects
Gerontology ,Endocrinology ,Index (economics) ,business.industry ,Endocrinology, Diabetes and Metabolism ,Pediatrics, Perinatology and Child Health ,Medicine ,Subject (documents) ,business - Published
- 2011
49. Sickness behavior and cytokine effects of a viral mimetic in aged mice
- Author
-
Dinko Kranjac, Kristina A. McLinden, Lauren E. Deodati, Michael J. Chumley, Marielle S. Kahn, and Gary W. Boehm
- Subjects
Behavioral Neuroscience ,Cytokine ,Endocrine and Autonomic Systems ,business.industry ,medicine.medical_treatment ,Immunology ,Medicine ,business ,Sickness behavior - Published
- 2010
50. STUDY OF THE DEPTH OF THE ANTERIOR CHAMBER—PHYSIOLOGICAL VARIATIONS WITH PARTICULAR EMPHASIS ON AMETROPIA
- Author
-
Calmettes, Deodati, Huron, and Bechac
- Subjects
Adult ,Male ,Adolescent ,Anterior Chamber ,business.industry ,Middle Aged ,Refractive Errors ,Ophthalmology ,Child, Preschool ,Humans ,Medicine ,Optometry ,Female ,France ,Child ,business ,Emphasis (typography) ,Aged - Published
- 1966
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.