1. Insulin-induced hypoglycemia stimulates gluconeogenesis from 14C-lactate independently of glucagon and adrenaline releases in rats
- Author
-
O. Mokuda and Yoshikazu Sakamoto
- Subjects
Blood Glucose ,Male ,Pentobarbital ,medicine.medical_specialty ,Epinephrine ,Endocrinology, Diabetes and Metabolism ,Radioimmunoassay ,Biology ,Peptide hormone ,Hypoglycemia ,Glucagon ,Endocrinology ,Internal medicine ,Internal Medicine ,medicine ,Animals ,Insulin ,Carbon Radioisotopes ,Lactic Acid ,Rats, Wistar ,Pancreatic hormone ,Gluconeogenesis ,General Medicine ,medicine.disease ,Rats ,Lactates ,medicine.drug ,Hormone - Abstract
Correlation between blood glucose, glucagon and adrenaline levels and gluconeogenesis was studied during the recovery from insulin-induced hypoglycemia in rats. Rats, overnight fasted, were intravenously injected with 40 microCi/kg of [U-14C]-lactate and 1 U/kg of porcine insulin under an anesthesia with pentobarbital sodium. Blood samples were drawn via the peripheral vein at 0, 5, 10 and 20 min. Plasma insulin level was 617 +/- 115 microU/ml at 5 min. Plasma glucose level was significantly decreased at 5 min (2.7 +/- 0.3 mM at 5 min v.s. 4.3 +/- 0.2 mM at 0 min, P < 0.01). Plasma glucagon and adrenaline did not significantly respond at 5 min, and then rised. Specific radioactivity of plasma [1-14C]-glucose was significantly higher at 5 and 10 min in the insulin-injected rat than the saline-injected rat (204 +/- 34 v.s. 130 +/- 14 d.p.m./mumol at 5 min, P < 0.01; 275 +/- 32 v.s. 186 +/- 16 d.p.m./mumol at 10 minm P < 0.01). These results suggest that lowering of blood glucose level stimulates gluconeogenesis independently of the release of counter-regulatory hormones in insulin-induced hypoglycemia.
- Published
- 2009