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Effect of various glucagon/insulin molar ratios on blood ketone body levels in rats by use of osmotic minipumps
- Source :
- Diabetes Research and Clinical Practice. 34:1-6
- Publication Year :
- 1996
- Publisher :
- Elsevier BV, 1996.
-
Abstract
- The bihormonal control by insulin and glucagon of blood ketone body level was studied. Mixed solutions with various molar ratios of glucagon and insulin (G/I) were subcutaneously infused continuously for five days by use of the osmotic minipump in the normal rats. The concentrations of insulin and glucagon solution were set at the high G/I molar ratio, the moderate G/I molar ratio and the low G/I molar ratio. In addition, the moderate G/I molar ratio group was divided into three sub-groups: low glucagon and low insulin, moderate glucagon and moderate insulin, and high glucagon and high insulin. After five days, the rats were decapitated to measure plasma ketone body, free fatty acid (FFA), glucose, insulin and glucagon. The FFA level was not significantly different among three groups. The glucose level was not different between the high and moderate G/I molar ratio groups, and decreased in the low G/I molar ratio group. 3-beta-hydroxybutyrate (3-OHBA) and acetoacetate (AcAc) levels in the high G/I molar ratio group were elevated, and 3-OHBA level in the low G/I molar ratio group was lowered compared to those in the moderate G/I molar ratio group. Among three moderate G/I molar ratio sub-groups, there was no difference in 3-OHBA and AcAc levels. These results demonstrate that plasma ketone body levels are controlled by the plasma G/I molar ratio.
- Subjects :
- Male
Molar
medicine.medical_specialty
Time Factors
Endocrinology, Diabetes and Metabolism
medicine.medical_treatment
Hydroxybutyrates
Ketone Bodies
Fatty Acids, Nonesterified
Glucagon
Acetoacetates
Endocrinology
stomatognathic system
Internal medicine
Diabetes mellitus
Internal Medicine
medicine
Animals
Insulin
Drug Interactions
Infusions, Parenteral
Rats, Wistar
Pancreatic hormone
chemistry.chemical_classification
3-Hydroxybutyric Acid
business.industry
Fatty acid
General Medicine
Metabolism
medicine.disease
Rats
chemistry
Ketone bodies
business
Subjects
Details
- ISSN :
- 01688227
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- Diabetes Research and Clinical Practice
- Accession number :
- edsair.doi.dedup.....6eb3974a4e6ae21db73564031fc7628f