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Abstract: Despite the strong antihepatotoxic, antioxidant, and antitumor properties of lignans from Schisandra chinensis, their applications in new drug development, bioscience and functional foods, etc. are limited because of their low abundance and complex coextractions. In this study, a magnetic separation method has been developed based on polyethylenimine‐modified magnetic nanoparticles to rapidly and effectively separate and purify the lignans from S. chinensis crude extracts through cation–π interaction and electrostatic adsorption. The magnetic nanoparticles were characterized by transmission electron microscopy, vibrating sample magnetometry, Fourier transform infrared spectroscopy, and X‐ray diffraction. Polyethylenimine‐modified magnetic nanoparticles showed a spherical‐shaped morphology and the average size was about 10 nm with superparamagnetism. Under the pH 7.4, polyethylenimine modified magnetic nanoparticles can remove a lot of coextracts. The range of detection limits and quantification limits was 0.27–0.34 and 0.89–1.13 ng/mL, respectively. Compared with other common methods, the magnetic separation method proposed in this study is much simpler and more effective through both strong cation–π interaction and electrostatic interaction. [ABSTRACT FROM AUTHOR]

• One hydroxynitrile glucoside, (R)-lotastralin is obtained by magnetic separation. • The BFMS serves separation of hydrophilic, hydrophobic components from plant extract. • This opens a route for enrichmenting hydroxynitrile glucoside in plant analysis. • Magnetic separations can be applied for a selective separation from plant extracts. • O. malacophyllus is much well-known that they contain harmful cyanogenic glycoside. A double benefit is a kind of strategy for separate efficiency. By selecting the appropriate method, the small molecule in plant extracts can be effectively extracted. CCK-8 method was employed to bioassay-guide. Magnetic separation was adopted as extraction probe to capture and enrich potent cytotoxicity constituent. Borate functionalized magnetic solid (BFMS) was performed for two purposes to wrap hydrophobic and to leave hydrophilic component from the ethyl acetate extract of O. malacophyllus , fortunately, (R)-lotastralin, 1- O -alpha-D-glucopyranosyl-beta-D-fructopyranose and 3- O -alpha-D-glucopyranosyl-beta-D-fructo furanose was obtained. As a result, O. malacophyllus is much well-known that they also contain hydroxynitrile glucoside. This provides a route to enrich hydroxynitrile glucoside in plant analysis. [ABSTRACT FROM AUTHOR]