Magnetic separation hydroxynitrile glucoside of Orostachys malacophyllus.

• One hydroxynitrile glucoside, (R)-lotastralin is obtained by magnetic separation. • The BFMS serves separation of hydrophilic, hydrophobic components from plant extract. • This opens a route for enrichmenting hydroxynitrile glucoside in plant analysis. • Magnetic separations can be applied for a selective separation from plant extracts. • O. malacophyllus is much well-known that they contain harmful cyanogenic glycoside. A double benefit is a kind of strategy for separate efficiency. By selecting the appropriate method, the small molecule in plant extracts can be effectively extracted. CCK-8 method was employed to bioassay-guide. Magnetic separation was adopted as extraction probe to capture and enrich potent cytotoxicity constituent. Borate functionalized magnetic solid (BFMS) was performed for two purposes to wrap hydrophobic and to leave hydrophilic component from the ethyl acetate extract of O. malacophyllus , fortunately, (R)-lotastralin, 1- O -alpha-D-glucopyranosyl-beta-D-fructopyranose and 3- O -alpha-D-glucopyranosyl-beta-D-fructo furanose was obtained. As a result, O. malacophyllus is much well-known that they also contain hydroxynitrile glucoside. This provides a route to enrich hydroxynitrile glucoside in plant analysis. [ABSTRACT FROM AUTHOR]