6 results on '"Guillot, Loïc"'
Search Results
2. DNA methylation at modifier genes of lung disease severity is altered in cystic fibrosis.
- Author
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Magalhães M, Rivals I, Claustres M, Varilh J, Thomasset M, Bergougnoux A, Mely L, Leroy S, Corvol H, Guillot L, Murris M, Beyne E, Caimmi D, Vachier I, Chiron R, and De Sario A
- Subjects
- Adult, Cystic Fibrosis blood, Epigenomics, Female, Glutathione Transferase genetics, Heme Oxygenase-1 genetics, Humans, Lung Diseases blood, Lung Diseases complications, Male, Nose chemistry, Receptor, Endothelin A genetics, Sequence Analysis, DNA, Sequence Deletion, Severity of Illness Index, Young Adult, Cystic Fibrosis genetics, Cystic Fibrosis Transmembrane Conductance Regulator genetics, DNA Methylation, Genes, Modifier, Lung Diseases genetics
- Abstract
Background: Lung disease progression is variable among cystic fibrosis (CF) patients and depends on DNA mutations in the CFTR gene, polymorphic variations in disease modifier genes, and environmental exposure. The contribution of genetic factors has been extensively investigated, whereas the mechanism whereby environmental factors modulate the lung disease is unknown. In this project, we hypothesized that (i) reiterative stress alters the epigenome in CF-affected tissues and (ii) DNA methylation variations at disease modifier genes modulate the lung function in CF patients., Results: We profiled DNA methylation at CFTR , the disease-causing gene, and at 13 lung modifier genes in nasal epithelial cells and whole blood samples from 48 CF patients and 24 healthy controls. CF patients homozygous for the p.Phe508del mutation and ≥18-year-old were stratified according to the lung disease severity. DNA methylation was measured by bisulfite and next-generation sequencing. The DNA methylation profile allowed us to correctly classify 75% of the subjects, thus providing a CF-specific molecular signature. Moreover, in CF patients, DNA methylation at specific genes was highly correlated in the same tissue sample. We suggest that gene methylation in CF cells may be co-regulated by disease-specific trans -factors. Three genes were differentially methylated in CF patients compared with controls and/or in groups of pulmonary severity: HMOX1 and GSTM3 in nasal epithelial samples; HMOX1 and EDNRA in blood samples. The association between pulmonary severity and DNA methylation at EDNRA was confirmed in blood samples from an independent set of CF patients. Also, lower DNA methylation levels at GSTM3 were associated with the GSTM3*B allele, a polymorphic 3-bp deletion that has a protective effect in cystic fibrosis., Conclusions: DNA methylation levels are altered in nasal epithelial and blood cell samples from CF patients. Analysis of CFTR and 13 lung disease modifier genes shows DNA methylation changes of small magnitude: some of them are a consequence of the disease; other changes may result in small expression variations that collectively modulate the lung disease severity.
- Published
- 2017
- Full Text
- View/download PDF
3. Moving beyond genetics: is FAM13A a major biological contributor in lung physiology and chronic lung diseases?
- Author
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Corvol H, Hodges CA, Drumm ML, and Guillot L
- Subjects
- Genome-Wide Association Study, Humans, Pulmonary Disease, Chronic Obstructive, Chronic Disease, GTPase-Activating Proteins genetics, GTPase-Activating Proteins physiology, Lung Diseases genetics, Lung Diseases physiopathology
- Abstract
Variants in FAM13A have been found in genome-wide association studies (GWAS) to associate with lung function in the general population as well as in several common chronic lung diseases (CLD) such as chronic obstructive pulmonary disease (COPD), asthma, as well as in idiopathic interstitial pneumonias (IIP). The gene was cloned in 2004, yet the encoded protein has not been characterised and its function is unknown. The redundancy of its genetic contribution in CLD suggests a major function of this gene both in lung physiology and CLD. This review provides a brief summary of the current knowledge of FAM13A, and demonstrates the necessity to resolve its biological function besides its well accepted genetic contribution. Further interpretations of FAM13A variants may help in the understanding of CLD mechanisms and reveal opportunity for intervention., (Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.)
- Published
- 2014
- Full Text
- View/download PDF
4. Multiplex Ligation-dependent Probe Amplification improves the detection rate of NKX2.1 mutations in patients affected by brain-lung-thyroid syndrome.
- Author
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Teissier R, Guillot L, Carré A, Morandini M, Stuckens C, Ythier H, Munnich A, Szinnai G, de Blic J, Clement A, Leger J, Castanet M, Epaud R, and Polak M
- Subjects
- Child, Child, Preschool, Female, Gene Deletion, Humans, Infant, Newborn, Male, Mutation, Nucleic Acid Amplification Techniques methods, Syndrome, Thyroid Nuclear Factor 1, Chorea genetics, Congenital Hypothyroidism genetics, Lung Diseases genetics, Nuclear Proteins genetics, Transcription Factors genetics
- Abstract
Background: NKX2.1 mutations have been identified in patients displaying complete or partial brain-lung-thyroid syndrome, which can include benign hereditary chorea (BHC), hypothyroidism and/or lung disease., Aims and Methods: We evaluated the recently developed Multiplex Ligation-dependent Probe Amplification (MLPA) method to assess the relative copy number of genes. The goal was to determine if MLPA could improve, in addition to direct sequencing, the detection rate of NKX2.1 mutations in a phenotype-selected cohort of 24 patients affected by neurological, thyroid and/or pulmonary disorders., Results: Direct sequencing revealed two heterozygous mutations. Using MLPA, we identified two further heterozygous NKX2.1 gene deletions. MLPA increased the detection rate by 50%. All patients with gene deletions identified were affected by BHC and congenital hypothyroidism., Conclusion: MLPA should be considered as a complementary tool in patients with partial or total brain-lung-thyroid syndrome when direct sequencing failed to identify NKX2.1 mutations. All patients with an NKX2.1 mutation had BHC and congenital hypothyroidism, emphasizing the high prevalence of these signs associated with defective NKX2.1 alleles., (Copyright © 2012 S. Karger AG, Basel.)
- Published
- 2012
- Full Text
- View/download PDF
5. BAL fluid surfactant protein C level is related to parenchymal lung disease in children with sarcoidosis.
- Author
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Guillot L, Flamein F, Thouvenin G, Boulé M, Ducou le Pointe H, Jonard L, Clement A, and Epaud R
- Subjects
- Adolescent, Biomarkers metabolism, Bronchi metabolism, Bronchi pathology, Child, Female, Humans, Lung Diseases pathology, Male, Sarcoidosis, Pulmonary pathology, Bronchoalveolar Lavage Fluid, Lung Diseases metabolism, Pulmonary Surfactant-Associated Protein C metabolism, Sarcoidosis, Pulmonary metabolism
- Published
- 2011
- Full Text
- View/download PDF
6. Macrolides: new therapeutic perspectives in lung diseases.
- Author
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Guillot L, Tabary O, Nathan N, Corvol H, and Clement A
- Subjects
- Airway Remodeling, Animals, Autophagy drug effects, Endoplasmic Reticulum Stress, Epithelium pathology, Epithelium physiopathology, Humans, Lung Diseases physiopathology, Pulmonary Alveoli pathology, Pulmonary Alveoli physiopathology, Pulmonary Surfactants metabolism, Regeneration, Lung Diseases drug therapy, Macrolides therapeutic use
- Abstract
There is strong clinical evidence for the effectiveness of macrolides in the treatment of a number of chronic airway diseases through their immunomodulatory effects. Recently, new information has been released supporting the view that macrolides may also be beneficial in pathologic situations associated with altered repair of the alveolar structure, such as those observed in interstitial lung diseases and fibrosis. It is proposed that macrolides may contribute to lung regeneration through their actions on several components of the remodeling process. The present review provides new insights on the effects of macrolides on the regenerative response of alveolar epithelium to injury. It also discusses novel findings which suggest that macrolides may contribute to alveolar surfactant homeostasis., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
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