19 results on '"dano hepático"'
Search Results
2. Síndrome de hiper-IgM con afectación hepática temprana.
- Author
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Guadalupe Coronado-Hernández, Kareli, Hugo Campos-Téllez, Héctor, Cortés-Grimaldo, Rosa María, Paola Macías-Robles, Ana, David Estrada-García, Carlos, Barrios-Díaz, Britza, Ramírez Nepomuceno, Adriana, Barreto-Alcalá, Marlén, Esparza-Amaya, David, Lilian Carvajal-Alonso, Hilda, and Berrón-Ruiz, Laura
- Abstract
Introduction: Hyper-IgM syndrome is an innate error of immunity in which there is a defect in change of isotype of immunoglobulins, with decreased values of IgG, IgA, and IgE, but normal or increased level of IgM. This predisposes to infectious processes at the respiratory and gastrointestinal levels, as well as autoimmune diseases and neoplasm. Case report: A 5 year 7-month-old boy with a history of 2 pneumonias, one of them severe, and chronic diarrhea since he was 2 years old. Persistent moderate neutropenia decreased IgG and elevated IgM. Cytometry flow confirmed absence of CD40L. Clinical evolution with early hepatic involvement. Discussion: Hyper-IgM syndrome predisposes to liver damage, so a complete evaluation is required as well as early diagnosis. Active anti-infective treatment and control of the inflammatory response are key to the treatment of liver damage. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
3. REVISÃO SISTEMÁTICA SOBRE DANOS HEPÁTICOS PROMOVIDOS PELA SUPLEMENTAÇÃO DE WHEY PROTEINS ATRAVÉS DOS BIOMARCADORES: ALANINA AMINOTRANSFERASE, ASPARTATO AMINOTRANSFERASE, GLICOSE, BILIRRUBINA, GAMA GLUTAMILTRANSFERASE, FOSFATASE ALCALINA, ALBUMINA E PROTEÍNAS TOTAIS EM RATOS E CAMUNDONGOS
- Author
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da Silva Barbosa, Anne Karynne, Navarro, Francisco, and Coppi Navarro, Antônio
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ALANINE aminotransferase , *ALKALINE phosphatase , *ALBUMINS , *PROTEINS , *BILIRUBIN , *WHEY proteins , *ASPARTATE aminotransferase - Abstract
Objective: To carry out a systematic review on the effects of whey protein supplementation on hepatic biomarkers Alanine Aminotransferase, Aspartate Aminotransferase, Glucose, Bilirubin, Alkaline Phosphatase, Gamma Glutamyltransferase, Albumin and Total Proteins in rats and mice. Materials and methods: Systematic Review, with the search words whey proteins; Alanine Aminotransferase, Aspartate Aminotransferase, Glucose, Bilirubin, Alkaline Phosphatase, Gamma Glutamyltransferase, Albumin and Total Proteins in Health Sciences Descriptors. the Capes Journal portal; Lilacs; Scielo.org; Scielo.br; dialnet; Redib; Medline. Results: of 222 eligible articles, 16 were used after exclusion criteria. Discussion: in summary, it was possible to observe studies with Whey Protein supplementation in which the biomarkers alanine aminotransferase, aspartate aminotransferase, glucose, bilirubin, alkaline phosphatase, gamma glutamyltransferase, albumin and total proteins were analyzed, detailing the models of the studies in experimentais procedures with rats. Conclusion: In conclusion, the data from this study suggest that supplementation dosages range from 0.15g to 40g/kg of Whey proteins, and from 0.15 to 20g/kg there are no changes in biochemical parameters related to liver damage. [ABSTRACT FROM AUTHOR]
- Published
- 2021
4. Liver manifestation associated with COVID-19 (Literature review).
- Author
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Joya, Shafiq Ahamd, Medeubaevna, Kurmanova Gaukhar, Dauletbayevna, Sadykova Assel, Nartbayevna, Tazhibayeva Karlygash, Nurlanovich, Bosat-bekov Erkebulan, and Abdurazakovna, Muratbekova Raikhan
- Subjects
COVID-19 ,CARDIOVASCULAR diseases ,SARS-CoV-2 ,SARS virus ,INTENSIVE care patients ,LITERATURE reviews ,LUNGS - Abstract
Copyright of Revista Latinoamericana de Hipertension is the property of Revista Latinoamericana de Hipertension and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2021
- Full Text
- View/download PDF
5. Stachyose combined with tea polyphenols mitigated metabolic disorders in high fructose diet-fed mice as studied by GC-MS metabolomics approach.
- Author
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Wu, Yingmei, Li, Wenfeng, Lu, Yalong, Wu, Qiu, and Yang, Xingbin
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STACHYOSE , *POLYPHENOLS , *METABOLIC disorders - Abstract
High fructose (HF) ingestion is a common risk factor for the development of nonalcoholic fatty liver disease (NAFLD), which has become a serious health problem. The underlying mechanism of HF-induced NAFLD needs to be further understood and an effective prevention needs to be established urgently. Gas chromatography-mass spectrometer-based metabonomic method was employed to characterize the serum metabolic profile of HF-induced NAFLD in mice. β-Hydroxybutyric acid, elaidic acid and oleic acid that contributed to energy and lipid metabolism were confirmed as potential biomarkers for HF-induced NAFLD. With these changed metabolic pathways as possible drug targets, different administrations revealed that co-administration of stachyose and tea polyphenols was a more effective method than treatment of stachyose or tea polyphenols alone for preventing HF-induced negative changes of metabolic pathways in mice. These findings suggest that β-hydroxybutyric acid, elaidic acid and oleic acid are potential biomarkers in HF-induced NAFLD, and co-administration is a novel preventive strategy. [ABSTRACT FROM AUTHOR]
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- 2018
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6. Systematic review on hepatic damage promoted by whey proteins supplementation through the biomarkers: alanine aminotransferase, aspartate aminotransferase, glucose, bilirubin, gamma glutamiltransferase, alkaline phosphatase, albumin and total proteins in
- Author
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Barbosa, Anne Karynne da Silva, Navarro, Francisco, and Navarro, Antonio Coppi
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Proteine totali ,Aspartato aminotransferasa ,Total proteins ,Proteinas de suero ,Proteinas totales ,Bilirrubina ,Albúmina ,Alkaline phosphatase ,Alanina Aminotransferase ,Gama glutamiltransferase ,Aspartato Aminotransferase ,Fosfatase alcalina ,Dano Hepático ,Glutamyltransferase ,Gamma glutamiltransferasi ,Proteínas totais ,Albumin ,Proteine del siero di latte ,Alanina aminotransferasi ,Bilirubin ,Alanine Aminotransferase ,Albumina ,Danno al fegato ,Whey Proteins ,Glucose ,liver damage ,Glucosio ,Glucosa ,Glicose ,Fosfatasa alcalina ,Gamma glutamiltransferasa ,Bilirubina ,Fosfatasi alcalina ,Aspartato aminotransferasi ,Daño hepático - Abstract
Objetivo: Fazer uma revisão sistemática sobre os efeitos da suplementação de whey proteins nos biomarcadores hepáticos Alanina Aminotransferase, Aspartato Aminotransferase, Glicose, Bilirrubina, Fosfatase Alcalina, Gama Glutamiltransferase, Albumina e Proteínas totais em ratos e camundongos. Materiais e métodos: Revisão Sistemática, com as palavras de busca whey proteins; Alanina Aminotransferase, Aspartato Aminotransferase, Glicose, Bilirrubina, Fosfatase Alcalina, Gama Glutamiltransferase, Albumina e Proteínas totais nos Descritores em Ciências da Saúde. o portal de Periódicos Capes; Lilacs; Scielo.org; Scielo.br; Dialnet; Redib; Medline. Resultados: de 222 artigos elegíveis utilizou-se 16 após critérios de exclusão. Discussão: em síntese, foi possível observar estudos com suplementação de Whey Proteins em que analisaram os biomarcadores alanina aminotransferase, aspartato aminotransferase, glicose, bilirrubina, fosfatase alcalina, gama glutamiltransferase, albumina e proteínas totais, detalhando os modelos dos estudos em procedimentos experimentais com ratos. Conclusão: Em conclusão, os dados deste estudo sugerem que as dosagens de suplementação variam entre 0,15g a 40g/kg de Whey proteins, sendo que de 0,15 a 20g/kg não ocorre alterações em parâmetros bioquímicos relacionados ao dano hepático. Objective: To carry out a systematic review on the effects of whey protein supplementation on hepatic biomarkers Alanine Aminotransferase, Aspartate Aminotransferase, Glucose, Bilirubin, Alkaline Phosphatase, Gamma Glutamyltransferase, Albumin and Total Proteins in rats and mice. Materials and methods: Systematic Review, with the search words whey proteins; Alanine Aminotransferase, Aspartate Aminotransferase, Glucose, Bilirubin, Alkaline Phosphatase, Gamma Glutamyltransferase, Albumin and Total Proteins in Health Sciences Descriptors. the Capes Journal portal; Lilacs; Scielo.org; Scielo.br; dialnet; Redib; Medline. Results: of 222 eligible articles, 16 were used after exclusion criteria. Discussion: in summary, it was possible to observe studies with Whey Protein supplementation in which the biomarkers alanine aminotransferase, aspartate aminotransferase, glucose, bilirubin, alkaline phosphatase, gamma glutamyltransferase, albumin and total proteins were analyzed, detailing the models of the studies in experimentais procedures with rats. Conclusion: In conclusion, the data from this study suggest that supplementation dosages range from 0.15g to 40g/kg of Whey proteins, and from 0.15 to 20g/kg there are no changes in biochemical parameters related to liver damage. Objetivo: Realizar una revisión sistemática sobre los efectos de la suplementación con proteína de suero de leche sobre los biomarcadores hepáticos Alanina Aminotransferasa, Aspartato Aminotransferasa, Glucosa, Bilirrubina, Fosfatasa Alcalina, Gamma Glutamiltransferasa, Albúmina y Proteínas Totales en ratas y ratones. Materiales y métodos: Revisión sistemática, con las palabras de búsqueda proteínas de suero; Alanina Aminotransferasa, Aspartato Aminotransferasa, Glucosa, Bilirrubina, Fosfatasa Alcalina, Gamma Glutamiltransferasa, Albúmina y Proteínas Totales en Descriptores de Ciencias de la Salud. el portal Capes Periodicals; lilas; Scielo.org; Scielo.br; dialnet; redib; Medline. Resultados: de 222 artículos elegibles, 16 fueron utilizados después de los criterios de exclusión. Discusión: en resumen, fue posible observar estudios con suplementación con Whey Protein en los que se analizaron los biomarcadores alanina aminotransferasa, aspartato aminotransferasa, glucosa, bilirrubina, fosfatasa alcalina, gamma glutamiltransferasa, albúmina y proteínas totales, detallando los modelos de los estudios en forma experimental. Procedimientos con ratas. Conclusión: En conclusión, los datos de este estudio sugieren que las dosis de suplementación oscilan entre 0,15g y 40g/kg de proteína de suero, y entre 0,15 y 20g/kg no hay cambios en los parámetros bioquímicos relacionados con el daño hepático. Objetivo: Fazer uma revisão sistemática sobre os efeitos da suplementação de whey proteins nos biomarcadores hepáticos Alanina Aminotransferase, Aspartato Aminotransferase, Glicose, Bilirrubina, Fosfatase Alcalina, Gama Glutamiltransferase, Albumina e Proteínas totais em ratos e camundongos. Materiais e métodos: Revisão Sistemática, com as palavras de busca whey proteins; Alanina Aminotransferase, Aspartato Aminotransferase, Glicose, Bilirrubina, Fosfatase Alcalina, Gama Glutamiltransferase, Albumina e Proteínas totais nos Descritores em Ciências da Saúde. o portal de Periódicos Capes; Lilacs; Scielo.org; Scielo.br; Dialnet; Redib; Medline. Resultados: de 222 artigos elegíveis utilizou-se 16 após critérios de exclusão. Discussão: em síntese, foi possível observar estudos com suplementação de Whey Proteins em que analisaram os biomarcadores alanina aminotransferase, aspartato aminotransferase, glicose, bilirrubina, fosfatase alcalina, gama glutamiltransferase, albumina e proteínas totais, detalhando os modelos dos estudos em procedimentos experimentais com ratos. Conclusão: Em conclusão, os dados deste estudo sugerem que as dosagens de suplementação variam entre 0,15g a 40g/kg de Whey proteins, sendo que de 0,15 a 20g/kg não ocorre alterações em parâmetros bioquímicos relacionados ao dano hepático. Obiettivo: effettuare una revisione sistematica degli effetti della supplementazione di proteine del siero di latte sui biomarcatori epatici alanina aminotransferasi, aspartato aminotransferasi, glucosio, bilirubina, fosfatasi alcalina, gamma glutamiltransferasi, albumina e proteine totali in ratti e topi. Materiali e metodi: Revisione Sistematica, con le parole di ricerca proteine del siero di latte; Alanina aminotransferasi, aspartato aminotransferasi, glucosio, bilirubina, fosfatasi alcalina, gamma glutamiltransferasi, albumina e proteine totali nei descrittori delle scienze della salute. il portale dei periodici Capes; lillà; Scielo.org; Scielo.br; rete telefonica; redib; Medline. Risultati: su 222 articoli ammissibili, 16 sono stati utilizzati dopo criteri di esclusione. Discussione: in sintesi, è stato possibile osservare studi con supplementazione di Whey Protein in cui sono stati analizzati i biomarcatori alanina aminotransferasi, aspartato aminotransferasi, glucosio, bilirubina, fosfatasi alcalina, gamma glutamiltransferasi, albumina e proteine totali, dettagliando i modelli degli studi sperimentali procedure con i topi. Conclusione: In conclusione, i dati di questo studio suggeriscono che i dosaggi di integrazione variano da 0,15 ga 40 g/kg di proteine del siero di latte e da 0,15 a 20 g/kg non ci sono cambiamenti nei parametri biochimici legati al danno epatico.
- Published
- 2022
7. Obtención y Análisis del Espectro de Absorción Óptico de Sangre de Rata Fisher con Daño Hepático Mediante Espectroscopia Fotoacústica.
- Author
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Alvarado-Noguez, M. L., Cano-Europa, E., Franco-Colín, M., Hernández-Aguilar, C., Domínguez-Pacheco, F. A., and Cruz-Orea, A.
- Abstract
Photoacoustic Spectroscopy (PAS) stand outs among Photothermal techniques due to its versatility for the characterization of different types of materials, including biological samples. In this study, were compared and analyzed by PAS, the differences between the optical absorption spectra of blood of male Fisher rats, in three cases, control (healthy rats), liver damage and liver damage treated with Curcuma longa. The experimental results show differences in the optical absorption spectra of each analysed case, therefore PAS would be proposed as a non conventional complementary technique, to study the effect of Curcuma longa in induced liver damage for an animal model. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
8. Fungos microscópicos recuperados de mel, isolamento e lesões patológicas por Penicillium sp em modelo experimental
- Author
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Marcos Davi Gomes Sousa, Maria Célia Pires Costa, Marcos Antonio Custódio Neto da Silva, Rebeca Costa Castelo Branco, Kátia Regina Assunção Borges, Walbert Edson Muniz Filho, Geusa Felipa de Barros Bezerra, and Maria do Desterro Soares Brandão Nascimento
- Subjects
Hongos microscópicos ,Microscopi fungi ,Penicillium sp ,Dano hepático ,General Earth and Planetary Sciences ,Honey ,Miel ,Daño hepático ,Liver damage ,Fungos microscópicos ,Mel ,General Environmental Science - Abstract
Background: Species of mycotoxin-producing fungi are potentially dangerous to humans and animals. The liver is the best-known organ of action of these substances. The aim of this study was to isolate microscopic fungi from honey and investigate the cytotoxic effect of the extract of Penicillium sp. in an experimental model. Methods: Honey samples were cultured in Sabouraud agar. After isolated and identified microscopically, the colonies of the genus Penicillium sp. were transplanted to the Sabouraud dextrose agar culture medium. After its development, they were processed to obtain an extract. Eighteen Wistar mice were randomly assigned to experimental (GI) and control (GII) groups. The GI was subjected to an oral inoculation of the extract, while GII received a placebo. Procedures were performed every day for thirty days, after which the liver of each animal was removed for analysis. Results: Aspergillus sp. (86.2%), Geotrichum sp. (6.89%) and Penicillium sp. (6.89%) were isolated. The most frequent species was Aspergillus niger (46%). In relation to the cytotoxic effects of the extract of Penicillium sp., the gross findings in the liver of GI suggested mainly congestion. Light microscopy showed that the little hepatic lobules were preserved and there was vascular congestion of sinusoids. Light microscopy of specimens from the experimental group showed that 68.2% were abnormal, whereas 87.5% of the control group were within normal limits. Conclusions: The results suggest that there was contamination in honey samples. There was a predominance of macroscopic and microscopic changes in the liver of experimental rats, suggesting liver damage by Penicillium sp. Antecedentes: Las especies de hongos productores de micotoxinas son potencialmente peligrosas para humanos y animales. El hígado es el órgano de acción más conocido de estas sustancias. El objetivo de este estudio fue aislar hongos microscópicos de la miel e investigar el efecto citotóxico del extracto de Penicillium sp. en un modelo experimental. Métodos: Las muestras de miel se cultivaron en agar Sabouraud. Luego de aisladas e identificadas microscópicamente, las colonias del género Penicillium sp. fueron trasplantados al medio de cultivo agar dextrosa Sabouraud. Luego de su desarrollo, fueron procesados para obtener un extracto. Se asignaron aleatoriamente dieciocho ratones Wistar a grupos experimentales (GI) y de control (GII). El GI se sometió a una inoculación oral del extracto, mientras que el GII recibió un placebo. Los procedimientos se realizaron todos los días durante treinta días, después de lo cual se extrajo el hígado de cada animal para su análisis. Resultados: Aspergillus sp. (86,2%), Geotrichum sp. (6,89%) y Penicillium sp. (6,89%) fueron aislados. La especie más frecuente fue Aspergillus niger (46%). En relación con los efectos citotóxicos del extracto de Penicillium sp., los hallazgos macroscópicos en el hígado de GI sugirieron principalmente congestión. La microscopía óptica mostró que los pequeños lóbulos hepáticos estaban conservados y había congestión vascular de los sinusoides. La microscopía óptica de las muestras del grupo experimental mostró que el 68,2 % eran anormales, mientras que el 87,5 % del grupo de control estaba dentro de los límites normales. Conclusiones: Los resultados sugieren que hubo contaminación en las muestras de miel. Hubo un predominio de cambios macroscópicos y microscópicos en el hígado de ratas experimentales, lo que sugiere daño hepático por Penicillium sp. Introdução: Espécies de fungos produtores de micotoxinas são potencialmente perigosas para humanos e animais. O fígado é o órgão de ação mais conhecido dessas substâncias. O objetivo deste estudo foi isolar fungos microscópicos do mel e investigar o efeito citotóxico do extrato de Penicillium sp. em um modelo experimental. Métodos: Amostras de mel foram cultivadas em ágar Sabouraud. Depois de isoladas e identificadas microscopicamente, as colônias do gênero Penicillium sp. foram transplantados para o meio de cultura Sabouraud dextrose agar. Após o seu desenvolvimento, foram processados para obtenção de um extrato. Dezoito camundongos Wistar foram distribuídos aleatoriamente nos grupos experimental (GI) e controle (GII). O GI foi submetido à inoculação oral do extrato, enquanto o GII recebeu placebo. Os procedimentos foram realizados diariamente durante trinta dias, após os quais o fígado de cada animal foi retirado para análise. Resultados: Aspergillus sp. (86,2%), Geotrichum sp. (6,89%) e Penicillium sp. (6,89%) foram isolados. A espécie mais frequente foi Aspergillus niger (46%). Em relação aos efeitos citotóxicos do extrato de Penicillium sp., os achados macroscópicos no fígado do GI sugeriram principalmente congestão. A microscopia de luz mostrou que os pequenos lóbulos hepáticos estavam preservados e havia congestão vascular dos sinusóides. A microscopia de luz dos espécimes do grupo experimental mostrou que 68,2% estavam anormais, enquanto 87,5% do grupo controle estavam dentro dos limites normais. Conclusões: Os resultados sugerem que houve contaminação nas amostras de mel. Houve predomínio de alterações macroscópicas e microscópicas no fígado de ratos experimentais, sugerindo lesão hepática por Penicillium sp.
- Published
- 2022
9. Long distance training associated to HIIT protocol does not induce changes in blood biochemical markers in adult marathoners
- Author
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Enrico Fuini Puggina, Camila de Moraes, Hugo Tourinho Filho, João Paulo Vieira Manechini, Pedro Mingoni Pimenta, and Rodrigo Aquino
- Subjects
Liver damage ,medicine.medical_specialty ,Health (social science) ,Thiobarbituric acid ,Physical Therapy, Sports Therapy and Rehabilitation ,030204 cardiovascular system & hematology ,medicine.disease_cause ,Blood biomarkers ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Daño muscular ,Ciencia deportiva ,Muscle damage ,Internal medicine ,medicine ,TBARS ,Biochemical markers ,Distance training ,Dano muscular ,medicine.diagnostic_test ,biology ,business.industry ,Dano hepático ,Biomarcadores sanguíneos ,030229 sport sciences ,Sports Science ,Ciências do Esporte ,Endocrinology ,chemistry ,GV557-1198.995 ,Serum iron ,biology.protein ,Creatine kinase ,business ,human activities ,Oxidative stress ,Daño hepático ,Sports - Abstract
Objective: to verify blood markers during a 12-week training protocol and after Sao Paulo Marathon. Methods: Blood samples of 9 male marathoners were collected before (C1) and after (C2) 12-week training protocol, before (C3) and after (C4) marathon. Muscle and liver damage markers (creatine kinase [CK-MM], aspartate aminotransferase [AST], alanine aminotransferase [ALT]), oxidative stress levels (thiobarbituric acid reactive substances [TBARS]) and serum iron concentration were measured. Results: changes were identified comparing moment C4 to other moments for CK-MM and iron. For AST, ALT, and TBARS no differences were identified. Conclusion: strenuous exercises might elicit changes on blood markers, needing follow up strategies to avoid impairments to athletes’ performance and health. RESUMO Objetivo: verificar marcadores sanguíneos durante 12 semanas de treinamento e após a Maratona de São Paulo. Metodologia: amostras de sangue de 9 maratonistas foram coletadas antes (C1) e após (C2) o treinamento de 12 semanas, antes (C3) e após (C4) a maratona. Marcadores sanguíneos e hepáticos (creatina quinase [CK-MM], aspartato aminotransf. [AST], alanina aminotransf. [ALT]), níveis de estresse oxidativo (subst. reativas ácido tiobarbitúrico [TBARS]) e ferro sérico foram analisados. Resultados: Verificaram-se alterações de CK-MM e ferro entre C4 e os outros momentos. Para AST, ALT e TBARS não se encontrou diferenças. Conclusão: exercícios exaustivos podem causar alterações em marcadores sanguíneos, requerendo estratégias de monitoramento para evitar danos ao desempenho e saúde do atleta. RESUMEN Objetivo: evaluar marcadores sanguíneos durante 12 semanas de entrenamiento y después del Maratón de São Paulo. Metodología: muestras de sangre de 9 maratonistas fueron recogidas antes (C1) y después (C2) el entrenamiento, y antes (C3) y después (C4) a maratón. Se analizaron marcadores sanguíneos y hepáticos (creatina quinasa [CK-MM], aspartato aminotransf. [AST], alanina aminotransf. [ALT]), estrés oxidativo (sustancias reactivas del ácido tiobarbitúrico [TBARS]) y de hierro. Resultados: Se encontraron cambios comparando C4 y otros momentos para CK-MM y hierro. Para AST, ALT y TBARS no se encontraron diferencias. Conclusión: ejercicios extenuantes pueden causar cambios en marcadores sanguíneos, requiriendo estrategias de monitoreo para evitar daños al desempeño y salud del atleta.
- Published
- 2020
10. Nori- and Sea spaghetti- but not Wakame-restructured pork decrease the hypercholesterolemic and liver proapototic short-term effects of high-dietary cholesterol consumption.
- Author
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Schultz Moreira, Adriana R., Benedi, Juana, Bastida, Sara, Sánchez-Reus, Isabel, and Sánchez-Muniz, Francisco J.
- Subjects
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PORK , *NORI , *HYPERCHOLESTEREMIA , *APOPTOSIS , *FUNCTIONAL foods , *LIVER diseases , *ALGAE products - Abstract
Restructured pork (RP) enriched in Seaweeds are potential functional foods. The antiapoptotic and hypocholesterolemic effects of consuming cholesterol enriched diets containing Wakame-RP (CW), Nori-RP (CN) and Sea Spaghetti (CS) were tested in a 1-wk study. Groups of six rats per group were fed a mix of 85% AIN-93M rodent-diet containing cholesterol and cholic acid as a cholesterol rising agent plus 15% RP containing alga. These diets were compared to control-RP diets enriched or not in cholesterol (CC and C, respectively). After 1-wk, cholesterol feeding significantly increased liver apoptosis markers which were significantly reduced by CS (cellular cycle DNA, caspase-3, and cytochrome c), CN (caspase-3 and cytochrome c) and CW (caspase-3) diets. CN and CS diets significantly blocked the cholesterolaemic rising effect observed in the CC group but no protective effect was observed in the CW group. Differences in seaweed composition added to RP appear responsible for blocking or not the proapoptotic and hypercholesterolemic effects of high cholesterol-RP consumption; thus, any generalization on seaweed effects or food containing seaweeds must be avoided. Although present results are worthy, future studies are demanded to ascertain the utility of consuming algal-RP as part of usual diets. [ABSTRACT FROM AUTHOR]
- Published
- 2013
- Full Text
- View/download PDF
11. Indicadores sanguíncos de daño hepático en novillos cruza cebú parasitados por Fasciola hepatica.
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Mussart, N. B. and Coppo, J. A.
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FASCIOLA hepatica ,ZEBUS ,CATTLE parasites ,LIVER diseases ,BEEF cattle diseases ,ASPARTATE aminotransferase ,FASCIOLIASIS ,CHOLESTASIS ,LEUCOCYTES ,DISEASES - Abstract
Copyright of Revista Veterinaria is the property of Universidad Nacional del Nordeste and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2009
- Full Text
- View/download PDF
12. Caracterización de la neuroinflamación en el hipocampo de ratas con daño hepático
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Sevilla Ferrer, Paloma
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Liver damage ,Neuroinflammation ,Inflamación periférica ,Tetracloruro de carbono ,Peripheral inflammation ,BIOQUIMICA Y BIOLOGIA MOLECULAR ,Grado en Biotecnología-Grau en Biotecnologia ,Carbon tetrachloride ,Encefalopatía hepática ,Daño hepático ,Neuroinflamación ,Hepatic encephalopathy - Abstract
[ES] La encefalopatía hepática (EH) es una alteración de la función cerebral consecuencia de un fallo en la función hepática previo. Existen dos tipos principales de fallo hepático: agudo y crónico, y ambos se producen por diferentes mecanismos y conllevan distintas consecuencias. Los pacientes con fallo hepático crónico (cirróticos en su mayor parte) sin síntomas evidentes de EH pueden presentar la llamada encefalopatía hepática mínima (EHM), la cual incluye ralentización psicomotora y déficits de atención entre otros síntomas que pueden ser detectados mediante tests psicométricos. La hiperamonemia y la inflamación periférica actúan sinérgicamente para producir estas alteraciones neurológicas. El propósito de este trabajo es estudiar en un modelo animal de la enfermedad, que se corresponde con ratas a las cuales se les administra tetracloruro de carbono para inducir el daño hepático, los mecanismos por los cuales esto ocurre. Se analizan cambios en el sistema inmune periférico, comprobando que hay un aumento en la expresión de CCL20 en el intestino de ratas con daño hepático, y si existe una relación con la neuroinflamación en el hipocampo. Para ello, se caracterizan las alteraciones en la expresión de IL-17 y TNF en el hipocampo debido al daño hepático, observándose una disminución de estas citoquinas en algunas regiones del hipocampo en ratas con daño hepático leve (esteatohepatitis). Conocer los mecanismos más tempranos por los que se induce la neuroinflamación en el hipocampo, la cual altera la neurotransmisión y la función cognitiva dependiente de esta área del cerebro, nos permitiría identificar dianas terapéuticas y proponer tratamientos que mejoren la función cognitiva en EHM., [EN] Hepatic encephalopathy (HE) is an alteration of brain function as a result of a previous liver failure. There are two main types of liver failure: acute and chronic, and both are produced by different mechanisms and carry different consequences. Patients with chronic hepatic failure (mostly cirrhotic) without evident symptoms of HE can present the so-called minimal hepatic encephalopathy (MHE), which includes psychomotor slowing and attention deficits among other symptoms that can be detected by psychometric tests. Hyperammonemia and peripheral inflammation act synergistically to produce these neurological alterations. The purpose of this work is to study in an animal model of the disease, which corresponds to rats given carbon tetrachloride to induce liver damage, the mechanisms by which this occurs. Changes in the peripheral immune system are analyzed, confirming that there is an increase in the expression of CCL20 in the intestine of rats with liver damage, and if there is a relationship with neuroinflammation in the hippocampus. To this end, alterations in the expression of IL-17 and TNF in the hippocampus due to liver damage are characterized, showing a decrease in these cytokines in some regions of the hippocampus in rats with mild hepatic damage (steatohepatitis). Knowing the earliest mechanisms by which neuroinflammation is induced in the hippocampus, which alters neurotransmission and cognitive function dependent on this area of the brain, would allow us to identify therapeutic targets and propose treatments that improve cognitive function in MHE.
- Published
- 2019
13. Evaluation of Baccharis trimera (Less) DC. (Asteraceae) protective effect on liver damage induced by carbon tetrachloride in rats
- Author
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Bonilha, Iuri de França, Manfron, Melânia Palermo, Bauermann, Liliane de Freitas, and Souto, Ricardo Bizogne
- Subjects
Liver damage ,Fígado ,Liver ,Baccharis trimera ,Tetracloreto de carbono ,Hepatoprotetor ,Dano hepático ,CIENCIAS DA SAUDE::FARMACIA [CNPQ] ,Hepatoprotective ,Carbon tetrachloride - Abstract
Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES The objective of this study was to evaluate the effect of Baccharis trimera hydroethanolic crude extract (BtHE), popularly known as carqueja, on CCl4 induced hepatic injury in Wistar rats. The liver is the main organ of metabolism and excretion of the human body, and is continually exposed to xenobiotics because of its strategic anatomical function. Liver diseases represent a significant health problem due to their limited complications and treatment possibilities. The phytotherapeutic researches for the treatment of hepatic disorders generally use animal models for the induction of hepatotoxicity, and the hepatic damage promoted by the administration of carbon tetrachloride (CCl4) is one of the most accepted and widespread models. Several species of Baccharis are widely used in folk medicine, mainly in the form of infusions, to treat various disorders, such as liver and gastrointestinal problems. The BtHE presented high levels of polyphenols (0.152 g GAE/100 g DW), flavonoids (19.07 g CE/100 g DW) and tannins (20.165 g CE/100 g DW) and a high antioxidant capacity, mainly for lipophilic substrates (59.23% inhibition of linoleic acid peroxidation). The extract promoted a slight synthesis of collagen in the liver, in agreement with the control group, and CCl4 did not promote significant fibrosis. In the evaluation of oxidative stress in hepatic tissue, the extract protected the liver from lipoperoxidation (300 mg/kg) and carbonylation of proteins (300 and 600 mg/kg) (p
- Published
- 2017
14. Changes in activity of some serum enzymes in rats with hypervitaminosis E
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Alarcón C, Oscar M and Giménez, Esther
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hipervitaminosis E ,alfa-tocoferol ,alpha-tocopherol ,liver damage ,enzimas séricas ,vitamina E ,vitamin E ,daño hepático ,serum enzymes - Abstract
Poco se sabe sobre los cambios en la actividad de las enzimas séricas relacionadas con la función hepática durante la hipervitaminosis E. En el presente trabajo se estudió el efecto de la administración intraperitoneal de 50, 100, 200 y 400 mg de vitamina E/día, durante 20 días sobre la actividad enzimática sérica en 60 ratas Wistar machos, de 12 semanas de edad, con pesos entre 180 y 200 gramos. El grupo control estuvo integrado por 15 ratas Wistar sanas, con edad y peso similares a los animales tratados. Al final del estudio, se tomaron muestras de sangre para la determinación de la vitamina E y la actividad de las enzimas: alanina aminotransferasa (ALT), aspartato aminotransferasa (AST), α-amilasa (AMS), arginasa (ARG), fosfohexosaisomerasa (PHI), fosfatasa alcalina (ALP), γ-glutamiltransferasa (γ-GT) y 5´-nucleotidasa (5´-N). La administración de vitamina E en exceso incrementó de manera significativa (p
- Published
- 2014
15. Nori- and sea spaghetti- but not wakame-restructured pork decrease the hypercholesterolemic and liver proapototic short-term effects of high-dietary cholesterol consumption
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Adriana R. Schultz Moreira, Juana Benedi, Sara Bastida, Isabel Sánchez-Reus, and Francisco J. Sánchez-Muniz
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Liver damage ,Meat ,Time Factors ,Swine ,Functional foods ,Hypercholesterolemia ,Apoptosis ,Undaria ,Alimentos funcionales ,Cholesterol, Dietary ,Functional Food ,Animals ,Rats, Wistar ,lcsh:RC620-627 ,Alga ,Porphyra ,Diet ,Rats ,Algas ,lcsh:Nutritional diseases. Deficiency diseases ,Cholesterol ,Liver ,Carne de cerdo reestructurada ,Colesterol ,Daño hepático ,Restructured pork - Abstract
Restructured pork (RP) enriched in Seaweeds are potential functional foods. The antiapoptotic and hypocholesterolemic effects of consuming cholesterol enriched diets containing Wakame-RP (CW), Nori-RP (CN) and Sea Spaghetti (CS) were tested in a 1-wk study. Groups of six rats per group were fed a mix of 85% AIN-93M rodent-diet containing cholesterol and cholic acid as a cholesterol rising agent plus 15% RP containing alga. These diets were compared to control-RP diets enriched or not in cholesterol (CC and C, respectively). After 1-wk, cholesterol feeding significantly increased liver apoptosis markers which were significantly reduced by CS (cellular cycle DNA, caspase-3, and cytochrome c), CN (caspase-3 and cytochrome c) and CW (caspase-3) diets. CN and CS diets significantly blocked the cholesterolaemic rising effect observed in the CC group but no protective effect was observed in the CW group. Differences in seaweed composition added to RP appear responsible for blocking or not the proapoptotic and hypercholesterolemic effects of high cholesterol-RP consumption; thus, any generalization on seaweed effects or food containing seaweeds must be avoided. Although present results are worthy, future studies are demanded to ascertain the utility of consuming algal-RP as part of usual diets.La incorporación de algas, ricas en fibra y compuestos asociados, a reestructurados de carne de cerdo (R) resulta en derivados cárnicos potencialmente funcionales. En este trabajo se estudian los efectos antiapoptóticos e hipocolesterolemiantes de dietas en las que se incluyen agentes hipercolesterolemiantes y R enriquecidos en diferentes algas, Wakame (RW), Nori-R (RN) y Espagueti de Mar (RE). Durante una semana grupos de seis ratas cada uno recibieron una mezcla constituida por 85% de dieta AIN- 93M para roedores suplementada con colesterol y ácido cólico, como agente hipercolesterolemiante, y 15% de RW, RN o RE. Estas dietas fueron comparadas con otras a las que se incorporó R control y a las que se añadió o no el agente hipercolesterolemiante. Después de 1 semana de tratamiento el incremento de marcadores de apoptosis hepática observado en el lote control con colesterol se redujo por el consumo de las dietas RE (DNA ciclo celular, caspasa-3, y citocromo c), RN (caspasa-3, y citocromo c) and RW (caspasa-3). Sólo las dietas con RN and RE bloquearon significativamente la inducción hipercolesterolemiante de la dieta control enriquecida en colesterol. Teniendo en cuenta las diferencias observadas entre los lotes respecto a sus efectos hipocolesterolémicos y antiapoptóticos, debe evitarse cualquier generalización sobre el consumo de algas y en particular de carnes conteniendo algas. Aunque los resultados son relevantes, deben realizarse estudios futuros para determinar la utilidad del consumo de estos R enriquecidos en algas dentro de dietas habituales.
- Published
- 2013
16. Liver damage hematological indicators in half–bred zebu steers parasitized by fasciola hepatica
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Mussart, Norma Beatriz and Coppo, José Antonio
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Novillo ,Bioquímica ,Liver damage ,Fasciolosis ,Hematología ,Hematology ,Biochemistry ,Daño hepático ,Steer - Abstract
Fil: Mussart, Norma Beatriz. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Fil: Coppo, José Antonio. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. El ensayo tuvo como objetivo indagar eventuales cambios de los indicadores sanguíneos de daño hepático durante la fase subclínica (asintomática) de la fasciolosis. Se seleccionaron 56 muestras de sangre de novillos cruza cebú aparentemente sanos, en mataderos de zonas enzoóticas de la Provincia de Corrientes (Argentina). Posteriormente al sacrificio se estableció que 29 de ellos albergaba escasa a moderada cantidad de duelas en el hígado, permaneciendo libres del parásito los restantes. Con técnicas convencionales de laboratorio se efectuó eritrograma, leucograma, proteinograma, enzimograma (fosfatasa alcalina ALP, gammaglutamil transferasa GGT, aspartato aminotransferasa AST) y determinaciones de hierro, glucosa, bilirrubina y tiempo de protrombina. Los animales parasitados revelaron valores más elevados de leucocitos, eosinófilos, gamma globulinas y GGT que los controles (p < 0,05), signos inflamatorios que se imputan a una incipiente colangitis, propia de la fase subclínica de la distomatosis. No se verificaron cambios atribuibles a colestasis ni insuficiencia hepática. The objective of the trial was to investigate eventual changes of hepatic damage blood indicators, during the subclinical (asymptomatic) period of fasciolosis. Fifty six blood samples of apparently healthy half–bred Zebu steers were collected in slaughterhouses from enzootic areas of Corrientes Province (Argentina). After slaughtering it was verified that 29 of them harbored scarce to moderate amount of liver flukes, being the rest of the animals free of parasites. Erythrogram, leukogram, proteinogram, enzymogram (alkaline phosphatase ALP, gammaglutamyl transferase GGT, aspartate aminotransferase AST) and iron, glucose, bilirubin and prothrombin time were determined by conventional laboratory techniques. Leukocyte, eosinophil, gamma globulin, and GGT values were higher in parasitized animals than in controls (p < 0.05). Such inflammatory signs are imputed to an incipient cholangitis, which is characteristic of the subclinical period of liver fluke disease. Changes attributable to cholestasis and hepatic failure were not verified.
- Published
- 2009
17. Hepatoprotective effect of 3-alkynyl selenophene against oxidative damage induced by chemical inductors in rats
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Wilhelm, Ethel Antunes, Savegnago, Lucielli, Nogueira, Cristina Wayne, Bonan, Carla Denise, and Mazzanti, Alexandre
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Liver damage ,Selenium ,Tetracloreto de carbono ,Dano hepático ,Selênio ,3-alquinil selenofeno ,2-nitropropane ,2-nitropropano ,Carbon tetrachloride ,CIENCIAS BIOLOGICAS::BIOQUIMICA [CNPQ] ,3-alkynyl selenophene - Abstract
Conselho Nacional de Desenvolvimento Científico e Tecnológico The liver presents extraordinary functional diversity, particularly in the control of energy production, immune defense and volemic reserve. The human being is exposed occupationally and in the environment to a variety of hepatotoxic compounds, such as the use of paints and their derivatives (2-nitropropane, 2-NP), chemical reagents (carbon tetrachloride, CCl4) and exposure to cigarette (2-NP). Therefore, it is interesting the study of therapies to prevent or even reverse the poisoning caused by these compounds. Considering that reactive oxygen species (ROS) have an important role in various diseases, especially in liver diseases, the use of antioxidant therapies should be considered. In this context, the heterocyclic compounds containing selenium in their structures have attracted the attention of researchers. Thus, this study investigated the antioxidant activity of 3-alkynyl selenophenes in models of oxidative damage in vitro and ex vivo in rats (Wistar, male, weighing 200-300g). A class of 3-alkynyl selenophene compounds with different substitutions was tested, with the objective to assess their antioxidant profile and their possible toxic effect in vitro. As a result, 3-alkynyl selenophenes had antioxidant activity, but this activity was dependent on the presence of terminal alkynes in the molecule or easy conversion to it. The possible toxic effect of 3-alkynyl selenophenes was evaluated through the activity of the enzyme δ-aminolevulinate dehydratase (δ-ALA-D) in vitro. The results showed that none of 3-alkynyl selenophenes inhibited the activity of this enzyme, suggesting that this class of compound did not present toxicity on this enzyme. From these results, selenophene h (compound that had the best antioxidant activity in vitro) was selected for the evaluation of its protective effect against oxidative damage induced by 2-NP and CCl4 (ex vivo). Selenophene h (25 mg/kg) protected against the increase of markers of liver damage (aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities) and oxidative stress induced by administration of 2-NP in rats. 2-NP induced microscopic changes, evaluated by histopathological inspections, that were protected by this compound. Selenophene h showed a protective effect against the increase of lipid peroxidation and inhibition of activity of δ-ALA-D in animals treated with 2-NP. Selenophene h protected against oxidative damage induced by CCl4 in rats. A single dose of CCl4 caused significant hepatotoxicity, evidenced by elevated plasma enzyme activity of AST and ALT, increased incidence of histopathological lesions, increased lipid peroxidation levels and the activity of Glutathione-S-transferase (GST), decreased levels of ascorbic acid and the activity of catalase and δ-ALA-D. In conclusion, 3-alkynyl selenophene protected from all these changes, confirming its hepatoprotective effect. Considering the results, we suggest that 3-alkynyl selenophene, an antioxidant, may be a useful therapy for the oxidative damage induced by 2-NP or CCl4 . O fígado apresenta extraordinária pluralidade funcional, destacando-se no controle de produção de energia, defesa imunológica e reserva volêmica. No meio ambiente e ocupacionalmente, o ser humano está exposto a uma variedade de compostos hepatotóxicos, como por exemplo, no uso de tintas e seus derivados (2-nitropropano, 2- NP), reagentes químicos (tetracloreto de carbono, CCl4) e na exposição ao cigarro (2-NP). Portanto, é interessante o estudo de terapias que previnam ou até mesmo revertam a intoxicação causada por estes compostos. Considerando que as espécies reativas de oxigênio (EROs) apresentam importante papel sobre diversas patologias, em especial nas doenças hepáticas, o uso de terapias antioxidantes deve ser considerada. Neste contexto, destacam-se os compostos heterocíclicos contendo selênio em sua estrutura. Deste modo, neste estudo investigou-se a atividade antioxidante de 3-alquinil selenofenos em modelos de dano oxidativo in vitro e ex vivo em ratos (Wistar, machos, pesando entre 200 300 g). Para esse fim, testou-se uma classe de compostos 3-alquinil selenofeno, com diferentes substituições na estrutura química, com o objetivo de avaliar o perfil antioxidante e seu possível efeito tóxico in vitro em ratos. Como resultado, 3- alquinil selenofenos tiveram atividade antioxidante, porém esta atividade foi dependente da presença de um alquino terminal na molécula ou da fácil conversão da molécula a um alquino terminal. Além disso, o possível efeito tóxico dos 3-alquinil selenofenos foi avaliado através da atividade da enzima δ-aminolevulinato desidratase (δ-ALA-D) in vitro. Os resultados obtidos demonstraram que nenhum dos 3-alquinil selenofenos testados inibiu a atividade desta enzima, sugerindo que esta classe de compostos não apresentou toxicidade sobre a atividade da δ-ALA-D. A partir destes resultados, selecionou-se o selenofeno h (que obteve melhor atividade antioxidante in vitro) para a avaliação do seu efeito protetor contra o dano oxidativo induzido por 2-NP e CCl4 em ratos (ex vivo). O selenofeno h (25 mg/kg) protegeu contra o aumento dos marcadores de dano hepático (aspartato aminotranferase (AST) e alanina aminotransferase (ALT)) e de estresse oxidativo induzidos pela administração do 2-NP. O 2-NP induziu alterações microscópicas avaliadas por inspeções histopatológicas as quais foram protegidas pelo composto. O selenofeno h demonstrou efeito protetor contra o aumento da peroxidação lipídica e inibição da atividade da δ-ALA-D nos animais tratados com 2-NP. Além disso, o selenofeno h protegeu contra o dano oxidativo induzido pelo CCl4 em ratos. Uma única dose de CCl4 causou significante hepatotoxicidade, evidenciada por elevação da atividade plasmática das enzimas AST e ALT, aumento da incidência de lesões histopatológicas, aumento dos níveis de peroxidação lipídica e da atividade da enzima glutationa-S-transferase (GST), bem como diminuição dos níveis de ácido ascórbico e da atividade das enzimas catalase e δ-ALA-D. A partir dos resultados demonstrados, verificou-se que o selenofeno h protegeu contra todas estas alterações, confirmando o seu efeito hepatoprotetor. Considerando os resultados obtidos, pode-se sugerir que o selenofeno h, uma molécula com atividade antioxidante, pode ser uma útil terapia contra o dano oxidativo induzido pelos hepatotoxicantes: 2-NP e CCl4.
- Published
- 2009
18. Effects of diphenyl diselenide administration on liver damage induced by 2-nitropropane, cadmium and carbon tetrachloride
- Author
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Borges, Lysandro Pinto, Zeni, Gilson Rogério, Nogueira, Cristina Wayne, Garcia, Solange Cristina, Lago, Lissandra Dal, Baldisserotto, Bernardo, and Mazzanti, Alexandre
- Subjects
Liver damage ,Selenium ,Diphenyl diselenide ,Tetracloreto de carbono ,Dano hepático ,Selênio ,Disseleneto de difenila ,2-nitropropane ,2-nitropropano ,Cádmio ,Carbon tetrachloride ,CIENCIAS BIOLOGICAS::BIOQUIMICA [CNPQ] ,Cadmium - Abstract
The liver presented exceptional characteristics, like controlling energy production, immunological defenses, and blood reserve. In the environment like in the work place, the human is exposed to a different kind of hepatotoxic compounds, for example, on inks and derivatives (2-nitropropane), chemical reagents (carbon tetrachloride) and in tobacco smoke (2-nitropropane and cadmium). In fact, is interesting studies of therapies which protect or ameliorated the damage induced by these compounds. Considering the growing interesting around organochalcogens, in special interest, diphenyl diselenide (PhSe)2, which posses important pharmacological properties, such as: anti-ulcer, antiinflammatory, antinociceptive, anti-hyperglycemic, protected against orofacial diskinesia induced by reserpine and halopheridol and may act on memory facilitation in mice, the hepatoprotective properties of this compound induced by different models of liver damage (2-nitropropane, cadmium and carbon tetrachloride) were examined. The results demonstrated that (PhSe)2 (100 µmol/kg) significantly reduced hepatic markers levels when compared to 2-nitropropane (2-NP) group. Treatment with diphenyl diselenide, at all doses, effectively protects against the increase of lipid peroxidation when compared to 2-NP group. In addition, histological examination revealed that 2-NP treatment causes a moderate swelling and degenerative alterations on hepatocytes and (PhSe)2 protects against these alterations. This study evidences the protective effect of diphenyl diselenide by 2-NP-induced acute hepatic damage. In addition the effect of post-treatment with (PhSe)2 on liver damage induced by 2-NP was also examined. (PhSe)2 effectively restored the increase of aminotransferase activities and urea level when compared to the 2-NP group. At the highest dose (100 mol/kg), (PhSe)2 decreased -glutamyl transferase activity (GGT) and ameliorated the increase of hepatic and renal lipid peroxidation when compared to 2-NP group. 2-NP reduced catalase activity (CAT) and did not alter superoxide dismutase activity (SOD) nor ascorbic acid level. This study points out the involvement of CAT activity in 2-NP-induced acute liver damage and suggests that the post-treatment with diphenyl diselenide was effective in restoring the hepatic damage induced by 2-NP. Similar results were obtained with cadmium (Cd), an environmental toxic metal implicated in human diseases. Cadmium content determined in the tissue of rats exposed to cadmium chloride (CdCl2) provides evidence that the liver is the major cadmium target. The concentration of cadmium in liver was about three fold higher than that in kidney, and (PhSe)2 reduced about six fold the levels of this metal in liver of rats exposed. Rats exposed to CdCl2 showed histological alterations abolished by (PhSe)2 administration. In addition, (PhSe)2 administration ameliorated plasma malondialdehyde (MDA) levels, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and GGT activities increased by CdCl2 exposure. In conclusion, this study demonstrated that co-treatment with (PhSe)2 ameliorated hepatotoxicity and cellular damage in rat liver after sub-chronic exposure with CdCl2. The proposed mechanisms by which (PhSe)2 acts in this experimental protocol are its antioxidant properties and its capacity to form a complex with Cd. On the contrary, the administration of (PhSe)2 potentiated acute hepatic damage induced by carbon tetrachloride (CCl4), as manifested by an increase in biochemical parameters (AST, ALT, ALP, GGT and BT) and severe alteration in histopathology. This study also demonstrated a potentiation of lipid peroxidation levels and a consequent depletion of important antioxidant defenses including catalase and ascorbic acid, suggesting that the oxidative damage is related to the potentiation effect induced by (PhSe)2. Considering the results obtained, could be suggested that (PhSe)2 present a hepatoprotective effect depending of experimental protocol. O fígado apresenta extraordinária pluralidade funcional, destacando-se no controle de produção de energia, defesa imunológica e reserva volêmica. No meio ambiente e ocupacionalmente, o ser humano está exposto a uma variedade de compostos hepatotóxicos, como por exemplo, no uso de tintas e seus derivados (2-nitropropano), reagentes químicos (tetracloreto de carbono) e na exposição ao cigarro (cádmio e 2-nitropropano). Portanto, é interessante o estudo de terapias que previnam ou até mesmo revertam à intoxicação causada por estes compostos. Considerando o crescente interesse por compostos orgânicos de selênio, em especial o disseleneto de difenila ((PhSe)2) que possui propriedades farmacológicas mais amplas como: efeitos anti-úlcera, antiinflamatório e antinociceptivo, anti- hiperglicemiante, protege contra a discinesia orofacial induzida por reserpina e haloperidol e pode atuar na facilitação da formação de memória em camundongos. Deste modo, os efeitos hepatoprotetores deste composto frente a diferentes modelos de dano hepático (2-nitropropano, cádmio e tetracloreto de carbono) foram examinados. Os resultados obtidos neste estudo demonstraram que a administração de (PhSe)2 (100 µmol/kg) reduziu os níveis de marcadores hepáticos e os níveis de peroxidação lipídica quando comparado ao grupo tratado com 2-nitropropano (2-NP). Além disso, os exames histológicos revelaram que o tratamento com 2-NP causou alterações degenerativas nos hepatócitos e que o (PhSe)2 foi capaz de proteger, evidenciando o efeito hepatoprotetor desse composto sobre o dano hepático induzido por 2-NP. O efeito do pós-tratamento com (PhSe)2 sobre o dano hepático induzido com 2-NP também foi investigado. Este composto restaurou a atividade plasmática das enzimas aminotransferases e os níveis de uréia quando comparado ao grupo tratado com 2-NP. Na maior dose (100 mol/kg), o (PhSe)2 causou uma diminuição na atividade da enzima -glutamil transferase (GGT) e restituiu o aumento nos níveis de peroxidação lipídica hepáticos e renais quando comparado ao grupo tratado com 2-NP. O tratamento com 2-NP reduziu a atividade hepática da catalase, entretanto não alterou a atividade da superóxido dismutase (SOD) e os níveis de ácido ascórbico, sugerindo que a inibição da CAT pode estar relacionada com o aumento nos níveis de peroxidação lipídica hepática nos ratos tratados com 2-NP. Resultados similares foram encontrados quando o dano hepático foi induzido por cádmio (Cd), um contaminante ambiental implicado em várias doenças. O conteúdo de Cd determinado nos ratos expostos ao cloreto de cádmio (CdCl2) provêm evidências de que o fígado é o maior alvo da toxicidade deste metal. A concentração de cádmio no fígado foi em torno de 3 vezes maiores que os níveis encontrados no rim. O (PhSe)2 reduziu em torno de 6 vezes os níveis deste metal no fígado dos ratos expostos ao CdCl2. Além disso, a administração de (PhSe)2 causou uma redução nos níveis de malondialdeído plasmáticos (MDA), na atividade das aminotransferases, na fosfatase alcalina (ALP), lactato desidrogenase (LDH) e GGT quando comparado ao grupo tratado com cádmio. Em conclusão, esse estudo demonstrou que o tratamento concomitante com (PhSe)2 reduziu a hepatotoxicidade e o dano celular em fígado de ratos expostos ao cádmio. O mecanismo proposto para ação do (PhSe)2 pode ser devido as suas propriedades antioxidantes ou pela sua capacidade de formar um complexo com Cd. Em contraste, a administração de (PhSe)2 potencializou o dano induzido por tetracloreto de carbono (CCl4), o que foi demonstrado pelo aumento dos níveis de marcadores bioquímicos (AST, ALT, ALP, GGT and BT) e pela severa alteração na histologia. Esses estudos também demonstraram que a administração de (PhSe)2 potencializou os níveis de peroxidação lipídica com consequente depleção das defesas antioxidantes, como a catalase e o ácido ascórbico, sugerindo que o dano oxidativo está relacionado com este efeito. Considerando os resultados obtidos, podemos sugerir que o disseleneto de difenila apresenta um efeito hepatoprotetor dependendo do modelo experimental.
- Published
- 2008
19. Hepatocytic differentiation of human bone marrow mesenchymal stem cells
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D. T. Covas, S. Kashima, A. M. Fontes, M. D. Orellana, F. C.L. Penteado, Universidade Estadual Paulista (Unesp), and Universidade de São Paulo (USP)
- Subjects
Liver damage ,cell migration ,alpha fetoprotein ,animal experiment ,lcsh:RS1-441 ,Hepatocyte differentiation ,animal cell ,paracrine signaling ,animal tissue ,reverse transcription polymerase chain reaction ,lcsh:Pharmacy and materia medica ,cell isolation ,immunophenotyping ,célula-tronco mesenquimal ,controlled study ,human ,normal human ,protein expression ,diferenciação hepatocítica ,albumina ,citoqueratina 18 ,dano hepático ,camundongos imunodeficientes ,albumin ,mesenchymal stem cell ,mouse ,Immunodeficient mice ,cytokeratin 18 ,nonhuman ,animal model ,human cell ,liver cell ,diferenciação ,cell differentiation ,real time polymerase chain reaction ,lcsh:HD9665-9675 ,lcsh:Pharmaceutical industry ,Mesenchymal stem cells ,hematopoietic stem cell ,immunofluorescence test ,cell structure ,liver injury - Abstract
Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:22:43Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:40:36Z : No. of bitstreams: 1 2-s2.0-50049118890.pdf: 1499773 bytes, checksum: a48cf5d73c37b720a2b37466fe2e688c (MD5) Made available in DSpace on 2014-05-27T11:22:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2007-12-01 Algumas pesquisas realizadas recentemente relatam que as células-tronco mesenquimais (CTM) podem ser induzidas à aquisição de marcadores hepatocíticos pelo transplante em modelos animais de dano hepático, ou pelo cultivo in vitro com fatores de crescimento e citocinas. O presente estudo teve por objetivo avaliar o comportamento das CTM frente à indução da diferenciação hepatocítica. As CTM foram isoladas da medula óssea de quatro doadores saudáveis, caracterizadas e submetidas ao protocolo de indução à diferenciação hepatocítica in vitro e in vivo. As células induzidas in vitro apresentaram mudanças na sua morfologia, mostrando a morfologia semelhante à do hepatócito, porém, o perfil imunofenotípico não foi modificado. As células induzidas também não apresentaram o aumento dos transcritos de albumina, citoqueratina 18 e citoqueratina 19 quando analisadas por RT-PCR em tempo real, e não alteraram a expressão de albumina, citoqueratina 18 e alfa-fetoproteína como demonstrado por imunofluorescência. Quando analisadas in vivo, as CTM demonstraram o potencial migratório para o tecido hepático danificado de camundongos imunodeficientes. Em conjunto, os resultados sugerem que as CTM da medula óssea não são capazes de se diferenciar em hepatócitos quando estimuladas in vitro pela metodologia utilizada neste estudo, mas são capazes de migrar para o tecido hepático danificado in vivo, o que sugere o seu papel no reparo do fígado. A contribuição para o reparo pode estar associada com o efeito parácrino dessas células. Palavras-chave: célula-tronco mesenquimal; diferenciação hepatocítica; albumina; citoqueratina 18; dano hepático; camundongos imunodeficientes Some recent articles have reported that mesenchymal stem cells (MSCs) can be induced to express hepatocyte markers by transplanting them into animal models of liver damage, or by in vitro culture with growth factors and cytokines. In this study, the aim is to evaluate the behavior of MSCs subjected to induction of hepatocyte differentiation. The MSCs were isolated from the bone marrow of 4 normal donors, characterized and subjected to both in vitro and in vivo induction of hepatocyte differentiation. The in vitro induced cells showed morphological changes, acquiring hepatocyte-like features. However, the immunophenotype of these cells was not modified. The induced cells exhibited no increase in albumin, cytokeratin 18 or cytokeratin 19 transcripts, when analyzed by real-time RT-PCR. The expression of albumin, cytokeratin 18 and alpha fetoprotein was also unchanged, according to immunofluorescence tests. In vivo, the MSC demonstrated a potential to migrate to damaged liver tissue in immunodeficient mice. Taken together, the results suggest that bone marrow MSCs are incapable of in vitro differentiation into hepatocytes by the approach used here, but are capable of homing to damaged hepatic tissue in vivo, suggesting a role for them in the repair of the liver. This contribution to tissue repair could be associated with a paracrine effect exerted by these cells. Departemente de Análises Clínicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Araraquara, SP Centro Regional de Hemoterapia Hospital Das Clínicas Universidade de São Paulo, USP, Ribeirão Preto, SP Departamento de Clínica Médica Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, USP, Ribeirão Preto, SP Centro Regional de Hemoterapia Hospital Das Clínicas Universidade de São Paulo, USP, Rua Tenente Catão Roxo 2501, CEP.: 14052-140 - Ribeirão Preto - SP Departemente de Análises Clínicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Araraquara, SP
- Published
- 2007
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