1. Optimization on preparation conditions of Rehmannia glutinosa polysaccharide liposome and its immunological activity.
- Author
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Huang Y, Wu C, Liu Z, Hu Y, Shi C, Yu Y, Zhao X, Liu C, Liu J, Wu Y, and Wang D
- Subjects
- Animals, Liposomes immunology, Lymphocytes drug effects, Lymphocytes immunology, Mice, Polysaccharides immunology, Liposomes chemistry, Polysaccharides chemistry, Rehmannia chemistry
- Abstract
The aim of this study was to investigate the optimizing preparation conditions and immunological enhancement activity of Rehmannia glutinosa polysaccharide liposome (RGPL). RGPL was prepared using the reverse-phase evaporation method and optimized using the response surface methodology. The immunological enhancement activity of RGPL on splenocyte proliferation was measured. These results showed that the optimum preparation conditions were: a soybean phosphatide to cholesterol ratio of 8:1, a chloroform to methanol ratio of 3:5, a soybean phosphatide to tween 80 ratio of 10:1, a temperature (°C) of 66°C and an entrapment efficiency of 72.753 ± 0.318%. In a single stimulation of drugs, RGPL could significantly promote splenocyte proliferation, specifically at 200 μg mL(-1). Moreover, in a synergistic stimulation of drugs with LPS or PHA, a significant difference was obtained between the RGPL and RGP at 100-400 μg mL(-1), which indicated that the immunological enhancement of RGP was significantly improved after encapsulation with the liposome., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
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