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Optimization on preparation condition of epimedium polysaccharide liposome and evaluation of its adjuvant activity.
- Source :
-
International journal of biological macromolecules [Int J Biol Macromol] 2012 Jan 01; Vol. 50 (1), pp. 207-13. Date of Electronic Publication: 2011 Oct 29. - Publication Year :
- 2012
-
Abstract
- The aim of this strategy was to investigate whether the adjuvant activity of epimedium polysaccharide (EPS) could be further enhanced after encapsulated with liposome. In preparation of EPS liposome (EPSL) test, an orthogonal L(9) (3(4)) test design was used to optimize the preparation condition of EPSL. In adjuvant activity test, 350 14-day-old chickens were randomly assigned to 7 groups and vaccinated with Newcastle disease (ND) vaccine. Simultaneously, the chickens in experimental groups were injected with EPSL at three doses, EPS and blank liposome, respectively. The activity of lymphocytes proliferation, titer of serum antibody and concentrations of cytokines were determined. Results showed that the optimal preparation condition of EPSL was that ratio of drug to lipid, ratio of soybean phospholipid to cholesterol, ultrasonic time, and water bath temperature were 1:30, 4:1, 10 min and 40°C, respectively. EPSL could significantly enhance the immune response of ND vaccine and promote cytokines secretion, and its high dose possessed the best efficacy. These findings indicated that liposome encapsulation could significantly improve the adjuvant activity of EPS.<br /> (Copyright © 2011 Elsevier B.V. All rights reserved.)
- Subjects :
- Animals
Cell Proliferation
Chickens
Cholesterol chemistry
Cytokines metabolism
Epimedium chemistry
Lipids chemistry
Lymphocytes cytology
Medicine, Chinese Traditional
Newcastle Disease metabolism
Newcastle disease virus metabolism
Phospholipids chemistry
Glycine max chemistry
Temperature
Adjuvants, Pharmaceutic chemistry
Liposomes chemistry
Polysaccharides chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0003
- Volume :
- 50
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- International journal of biological macromolecules
- Publication Type :
- Academic Journal
- Accession number :
- 22074742
- Full Text :
- https://doi.org/10.1016/j.ijbiomac.2011.10.021