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We have evaluated the ameliorative effect of ginger extract on paraben (p-hydroxybenzoic acid)-induced lipid peroxidation in the liver of mice. Adult female albino mice were orally administered with 2.25 or 4.50 mg of paraben in 0.2 mL olive/animal/day (67.5 and 135 mg/kg of body weight) for 30 days. The results revealed significantly higher (p < or = 0.05) lipid peroxidation in the liver of paraben-treated mice than that of controls. As compared with the controls, the levels of non-enzymatic antioxidants: glutathione and ascorbic acid, as well as the enzymatic antioxidants: superoxide dismutase, glutathione peroxidase and catalase were significantly (p < or = 0.05) lowered in the liver of paraben-treated mice. Oral administration of aqueous extract of Zinziber officinale (3 mg/animal/day) along with paraben for 30 days (Groups 6 and 7) caused significant (p < or = 0.05) amelioration in paraben-induced lipid peroxidation and increased significantly (p < or = 0.05) the activities of enzymatic (superoxide dismutase, glutathione peroxidase, catalase) and contents of non-enzymatic (glutathione and ascorbic acid) antioxidants in the liver of mice, as compared with those given paraben alone (Groups 4, 5). Thus, oral administration of aqueous extract of Zinziber officinale along with paraben significantly (p < or = 0.05) ameliorates paraben-induced lipid peroxidation in the liver of mice.

The present investigation was an attempt to evaluate the ameliorative effect of curcumin on aflatoxin-induced lipid peroxidation in liver and kidney of mice. Aflatoxin was obtained by growing Aspergillus parasiticus in SMKY liquid medium. Pure curcumin (97% purity) was purchased from Hi-Media Laboratories Pvt. Ltd., Mumbai, India. Young adult male albino mice were orally administered with low dose and high dose (750 and 1500 microg/kg body weight) with and without curcumin (2 mg/0.2 mL olive oil/animal/day) for 45 days. On 46th day the animals were sacrificed by cervical dislocation. Liver and kidney were removed and weighed. Homogenates were prepared for measuring lipid-peroxidation along with changes in catalase, superoixide dismutase, glutathione, glutathione-peroxidase and total ascorbic acid. The results revealed concentration dependent increase in lipid peroxidation along with reduction in enzymatic and non-enzymatic antioxidants. Treatment with curcumin along with aflatoxin ameliorates aflatoxin-induced lipid peroxidation in liver and kidney of mice by ameliorating both enzymatic and non-enzymatic antioxidants.

The present investigation was an attempt to evaluate the possible ameliorative effect of curcumin on aflatoxin-induced lipid peroxidation in liver, kidney and testis of mice in vitro. Tissues were collected from healthy Swiss strain male albino mice Mus musculus weighing 30-35 g. The homogenates were treated with aflatoxin (2-10 mg/mL) with and without curcumin (25-200 mg/mL). The results revealed that addition of aflatoxin (2-10 mg/mL) to homogenates caused significant increase in lipid-peroxidation which was maximal at 6 mg/mL aflatoxin concentration. However, concurrent addition of aflatoxin (6 mg/mL) and curcumin ((25-200 mg/mL) caused concentration-dependent amelioration in aflatoxin-induced lipid peroxidation.

Antioxidative effect of aqueous extract of rhizome of Ginger (Zinziber officinale) was examined on p-hydroxybenzoic acid (paraben) induced lipid peroxidation. Addition of paraben (25-150 microg/mL) to liver and kidney homogenates significantly increases H2O2 induced lipid peroxidation in vitro. Effect was dose dependent up to 100 microg/mL concentration. An addition of aqueous extract of ginger significantly reduced paraben (100 microg/mL) induced lipid peroxidation in liver and kidney homogenates. The effect was concentration dependent.

Oral administration of 25 and 50 mg of aflatoxin in 0.2 mL olive oil/animal/day for 30 days caused dose-dependent and significantly higher lipid peroxidation in the kidney of aflatoxin-treated mice than in the controls. The levels of non-enzymatic antioxidant such as glutathione as well as the enzymatic antioxidants such as superoxide dismutase, glutathione peroxidase and catalase were significantly lower in the kidney of aflatoxin-treated mice than in the controls. Black tea extract (2%) treatment significantly ameliorates aflatoxin-induced lipid peroxidation, which could be due to higher enzymatic and non-enzymatic antioxidants in the kidney of mice as compared with that given aflatoxin alone. These findings suggest that black tea extract treatment significantly ameliorates aflatoxin-induced lipid peroxidation in the kidney of mice.

We have evaluated the ameliorative effect of black tea extract on aflatoxin-induced lipid peroxidation in the liver of mice. Adult male albino mice were orally administered with 25 and 50 microg of aflatoxin in 0.2 ml olive oil/animal/day for 30 days. Results revealed dose-dependent and significantly (p<0.05) higher lipid peroxidation in the liver of aflatoxin-treated mice than that of vehicle control. As compared with vehicle control, the levels of non-enzymatic antioxidants such as glutathione and ascorbic acid, as well as the enzymatic antioxidants such as superoxide dismutase, glutathione peroxidase and catalase were significantly (p<0.05) lowered in the liver of aflatoxin-treated mice. Oral administration of two percent aqueous black tea extract along with aflatoxin for 30 days (groups 6 and 7) caused significant (p<0.05) amelioration in aflatoxin-induced lipid peroxidation by increasing significantly (p<0.05) the activities of enzymatic (superoxide dismutase, glutathione peroxidase, catalase) and contents of non-enzymatic (glutathione and ascorbic acid) antioxidants in the liver of mice as compared with those given aflatoxin alone (groups 4 and 5). Thus, oral administration of black tea along with aflatoxin significantly (p<0.05) ameliorates aflatoxin-induced lipid peroxidation in the liver of mice.

Aim: To evaluate the ameliorative effect of vitamin E on aflatoxin-induced lipid peroxidation in the testis., Methods: Adult male albino mice were orally administered 25 or 50 microg of aflatoxin in 0.2 mL olive oil per d for 45 d. The testis was isolated, blotted free of blood and processed for biochemical analysis., Results: There was a dose-dependent significantly higher lipid peroxidation in the testis of aflatoxin treated mice than in the controls. The levels of non-enzymatic antioxidants such as glutathione, total and reduced ascorbic acid, as well as the activities of enzymatic antioxidants, such as superoxide dismutase, glutathione peroxidase and catalase were significantly lower in the testis of aflatoxin treated mice. Vitamin E (2 mg/d per animal; orally) pretreatment significantly ameliorates the aflatoxin-induced lipid peroxidation which could be due to higher enzymatic and non-enzymatic antioxidants in the testis of mice as compared with those given aflatoxin alone., Conclusion: Vitamin E pretreatment significantly ameliorates aflatoxin-induced lipid peroxidation in the testis of mice.