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Bovine split hide is the subcutis layer with the low percentage of collagen so that the quality of the gelatin is different from that of the gelatin from cattle’s skin. This study aims to improve the characteristics of bovine split hide gelatin combined with soy protein isolate (SPI) using transglutaminase (TGase) enzyme as a protein cross-linking agent. The study was conducted using a completely randomized design with 3 x 3 factorial pattern consisting of three levels of a mixture of bovine split hide gelatin : SPI at the ratios of 90 : 10, 80 : 20, 70 : 30, and three concentrations of transglutaminase enzyme, i.e. 10, 20, and 30 U. The results showed that treatments significantly affected viscosity, gel strength, the moisture, ash, fat, and protein content of gelatin. Electrophoresis of gelatin protein showed bands distribution between 60-190 kDa. The amino acid profile of the gelatin was similar to that of collagen with a high level of aspartate, glutamate, cysteine, and proline. Morphology of gelatin was observed by Scanning Electronic Microscope (SEM) and showed a compact distributed collagen crosslink. The combination of gelatin bovine split hide and SPI at the ratio of 90 : 10 provides the best physicochemical characteristic.

The characteristic of chicken excreta has a very potential as a breeding media for flies and is known for causing odorous pollutants (NH3 emission) from undigested protein and the activities of urease microorganisms. This study is focused on extracting Maja fruit, to quantify marmelosin from different fruit conditions using HPLC, and to determine the biological activity for handling the chicken excreta problems. In this study, the Kirby-Bauer Test was used to observe the antibacterial activity of marmelosin, the NH3 trapping method was used to determine ammonia emission, and the larvae population was determined by the Fly-Grill method. Marmelosin contents in MFE from immature, mature, and fermented fruit condition were 108.65 μg/g; 65.83 μg/g, and 23.02 μg/g, respectively. The increasing level of marmelosin addition to 50, 100, 150, and 200 μg/mL caused the higher diameter of inhibition zone against E. coli (p

Contamination that decreased chicken meat quality could be prevented using natural preservatives. Bay leaves (Syzygium polyanthum) contain volatile fatty acids, tannin, and flavonoid that possess bacteriological and fungicidal activity as well as preventing bacterial spore growth. The purpose of this study was to determine the effect of fresh bay leaf infusion on microbiological, chemical, and physical qualities of chicken meat. This study used bay leaves, water, chicken meat, eight strain of bacteria, chemicals and materials for the analysis of chicken meat. The experiment consisted of two steps, the first was the antibacterial properties of bay leaves and the second was the application of bay leaf infusion for chicken meat. Eight bacteria was used for the bacterial inhibition of bay leaf at the concentration of 0, 5, 10, 15 and 20%. The experiment on antibacterial properties of bay leaf (Syzygium polyanthum) used one way randomized design with five concentration treatments, while the application of bay leaf infusions on chicken meat using factorial completely randomized design 2x5 (2 types of soaking and 5 observation time). At the second step, chicken meat was divided into 2 groups, the first group was soaked in water only and the second group was soaked in 15% bay leaf infusion. They were then stored for 0 (control), 2, 4, 6, and 8 hours at the room temperature. Each treatment was repeated four times. The microbiological, chemical, physical qualities of chicken meat were observed. The results showed that bay leaf infusion had the ability to inhibit the growth of bacteria (P

This study examined the characteristics of the edible film mixture gelatin bovine split hide and soy protein isolate, cross-linked using transglutaminase enzyme with addition level of glycerol as a plasticizer.The material used gelatin combination (Gelatin bovine split hide: soy proteinisolate = 90: 10 with 30 Utransglutaminase enzyme levels ), and glycerol. Data were analyzed using completely randomized design each treatment was replicated thrice of glycerol as a treatment level ( 10%, 20% dan 30%). The results showed that the glycerol level significantly affected the thickness, elongation, solubility, degree of cross-linking and WVTR, but not the tensile strength.The addition of glycerol causing the film becomes more elastic and the surface is more homogeneous and compact. Increasing the level to 30% glycerol affects the mechanical properties of an edible film.

This research aimed to investigate the rabbit meat hydrolysate potential as an angiotensin-converting enzyme (ACE) inhibitor. Indonesian local rabbit meats were used in this study. The research was conducted in Department of Animal Product Technology, Faculty of Animal Science, Universitas Gadjah Mada, from August 2016 to February 2017. The local rabbit meats were hydrolyzed by pepsin, trypsin, and pancreatic. The obtained hydrolysates were then analyzed to identify the water-soluble protein content. The molecular weight of the hydrolysates were also confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The ACE inhibitory properties of the hydrolysates were analyzed in vitro. The results showed that pepsin, trypsin, and pancreatic hydrolysis showed a significant effect on the water-soluble protein content of rabbit meat (p

The objective of this study was to evaluate the application of the use optimization methods of Simplex Lattice Design (SLD) with special cubic models in the preparation formula of hard capsule based on goatskin gelatin. Three types of filler materials have been used in the manufacture of capsule shells, namely MgCO3, tapioca starch (TS) and sago starch (SS). The basic ingredient was uses goatskin gelatin and aquadest as a solvent. The material formulation was calculated according to Simplex Lattice Design (SLD) using the equations Y = β1 (A) + β2 (B) + β3(C) + β12 (A)(B) + β13 (A)(C) + β23 (B)(C) + β123 (A)(B) (C). Based on these equations obtained seven formulas (three proportions formula 100% each component, three proportional formulas 50%: 50% for the mixture of two components and one formula 33.33% for the mixture of three components). The results obtained data related to the proportion of filler use in a mixture of materials. The superimposed contour plot shows that the proportion of the use of three types of filler (MgCO3: TS: SS) in each mixture are (0.224 part: 0.055 part and 0.721 parts). Next, after further testing of the formula is then obtained properties of the capsule shell prototype, namely: thickness (0.35 mm), solubility (66.64%), and water vapor transmission rate (WVTR) (0.67 g.H2O.m-2 h-1). The data obtained that the type of SS filler is the most dominant factor influencing in increasing the thickness and solubility properties of the capsule shell, while the filler TS is the most dominant increase in the nature of WVTR. The results of the study concluded that the application of the SLD optimization method could be applied in the preparation of hard capsule formulations made from goatskin gelatin with better properties.

Methanol dehydrogenase (MDH) enzyme was purified from Bradyrhizobium japonicum USDA110 cell-free extract. The bacteria were grown in a culture medium with direct 0.5% methanol addition aimed to stimulates the MDH catalytic enzyme activation. Bradyrhizobium japonicum USDA110 MDH enzyme was purified by using 25 mM 2-(N-morpholine) ethanesulfonic acid/MES pH 5.5 buffer and 1 M sodium chloride/NaCl which separated into three columns, the first column was PD-10 for buffer exchange; the second column was HiTrap Sepharose HP to obtain unbonded fraction in the column; and the third column was Mono S 5/50 GL integrated with two pumps HPLC (high-performance liquid chromatography) to obtain pure MDH enzyme for serial changing of 1 M NaCl-25mM MES pH 5.5 with the flow rate at 1 ml/min. The protein concentration and MDH catalytic enzyme activity were observed on each purification process starting from the cell-free extract to pure MDH enzyme. The pure MDH enzyme was obtained by Mono S 5/50 GL-HPLC purification which showed a single band on SDS PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). The MDH enzyme purification from Bradyrhizobium japonicum USDA110 showed 90-fold purification, a sub-molecular weight of 63 kDa, specific activity at 2.69 U/mg, and optimum activity at a 35oC temperature and pH 9.

This research aims to understand the effect of metal ions bioabsorption which belong on different elemental groups to the methanol dehydrogenase (MDH) enzymatic activity in nitrogen-fixing bacteria Bradyhizobium japonicum USDA 110. Ten metal ions with each have 30μM concentration were added to grow Bradyhizobium japonicum USDA 110 in 10-1 diluted nutrient medium. The MDH activity test showed a similar result between the bacteria grown in medium without metal ions addition (control) and the bacteria were grown in a calcium ion/Ca2+ added media. The highest MDH enzymatic activity was shown on the bacteria grown in a magnesium/Mg2+ added medium, which showed 0.08 (U/mg) enzymatic activities. The addition of magnesium/Mg2+ metal ion accelerates the bacterial growth by 2.6 times and MDH activity by 1.28 times compared to control. The MDH enzyme is essential, especially for bacteria which exist in the soil environment, to adapt to high methanol concentration and to support bacterial anaerobic growth capacity along with plant symbiotic process. Moreover, the MDH activity staining method could also act as pollutant indicators like metal ions and hydrocarbon derivates. This research concluded that metal ions biosorption (calcium/Ca2+ and magnesium/Mg2+) are required for bacterial cells reproduction and oxidation of single carbon chain compounds like methanol. The nitrogen-fixing symbiotic bacteria, Bradyhizobium japonicum USDA 110 showed high MDH activity after the two metal ions absorption. However, contrary results were shown on vanadium/V3+, manganese/Mn2+, iron/Fe3+, copper/Cu2+, zinc/Zn2+, and aluminum/Al3+ absorption, which showed low MDH activity and cells biomass.

The experiment was conducted to investigate the angiotensin converting enzyme (ACE) inhibitory activity of hydrolysate in meat protein of Bali cattle, Kacang goat, native chicken, and local duck. The meats of Bali cattle, Kacang goat, native chicken, and local duck were used in this study. The meats were ground using food processor added with aquadest to obtain meat extract. The meat extracts were then hydrolyzed using protease enzymes to obtain hydrolysate of meat protein. Protein concentration of meat extract and hydrolysate of meat protein were determined, and were confirmed by sodium dodecyl sulfate - poly acrylamide gel electrophoresis (SDS-PAGE). ACE inhibitory activity of hydrolysate of meat protein derived from Bali cattle, Kacang goat, native chicken, and local duck was also determined. The results showed that protein concentration of hydrolysate of meat protein of Bali cattle, Kacang goat, native chicken, and local duck meat was significantly higher than their meat extracts. SDS-PAGE analysis indicated that hydrolysate of meat protein of Bali cattle, Kacang goat, native chicken, and local duck had more peptides with lower molecular weight, compared to their meat extracts. Hydrolysate of meat protein of Bali cattle, Kacang goat, native chicken, and local duck had potencies in inhibiting ACE activity, so it will potentially reduce blood pressure.

This research applied and evaluated a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using cytochrome b gene to detect pork contamination in meatballs from local markets in Surabaya and Yogyakarta regions, Indonesia. To confirm the effectiveness and specificity of this fragment, thirty nine DNA samples from different meatball shops were isolated and amplified, and then the PCR amplicon was digested by BseDI restriction enzyme to detect the presence of pork in meatballs. BseDI restriction enzyme was able to cleave porcine cytochrome b gene into two fragments (131 bp and 228 bp). Testing the meatballs from the local market showed that nine of twenty meatball shops in Yogyakarta region were detected to have pork contamination, but there was no pork contamination in meatball shops in Surabaya region. In conclusion, specific PCR amplification of cytochrome b gen and cleaved by BseDI restriction enzymes seems to be a powerful technique for the identification of pork presence in meatball because of its simplicity, specificity and sensitivity. Furthermore, pork contamination intended for commercial products of sausage, nugget, steak and meat burger can be checked. The procedure is also much cheaper than other methods based on PCR, immunodiffusion and other techniques that need expensive equipment.

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Penelitian bertujuan untuk mengetahui pengaruh penggunaan minyak buah merah (MBM) pada pakan terhadap penampilan produksi ayam broiler. Delapan puluh ekor ayam broiler umur sehari (DOC) ditempatkan pada empat kelompok perlakuan pakan yang berbeda, yaitu: P0 (pakan kontrol/tanpa penambahan minyak), P1 (2% MBM), P2 (4% MBM), dan P3 (6% MBM). Setiap kelompok perlakuan terdiri dari empat ulangan masing-masing dengan lima ekor. Ayam broiler dipelihara selama 41 hari. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan analisis variansi pola searah dan diuji lanjut dengan Duncan’s New Multiple Range Test (DMRT) . Hasil penelitian menunjukkan bahwa konsumsi pakan berbeda nyata (P

Penelitian ini bertujuan untuk mengetahui pengaruh jus daun sirih (Piper betle Linn) pada proses precuring dan lama penyimpanan terhadap komposisi kimia dan angka peroksida dendeng ayam petelur. Daging direndam jus daun sirih (0, 5, 10, dan 15%) kemudian diolah menjadi dendeng. Produk dendeng disimpan pada suhu kamar selama 0, 2, 4, 6, dan 8 minggu. Variabel yang diukur meliputi kadar air, kadar protein, kadar lemak, pH, dan angka peroksida. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap pola faktorial 4 x 5 dan dilanjutkan dengan uji Tukey. Hasil penelitian menunjukkan bahwa semakin tinggi konsentrasi jus daun sirih berpengaruh nyata (P(Pmempertahankan komposisi kimia secara proposional dan menekan angka peroksida sehingga memperpanjang lama penyimpanan dendeng ayam petelur afkir.(Kata kunci: Daging ayam, Daun sirih, Dendeng, Komposisi kimia, Angka peroksida)

Application of strong bases as curing materials has been widely applied in commercial gelatin industries, but the application of weak bases has not been much done. Application of strong bases as a treatment was not economical and assumed to affect human health. Studies were conducted on the properties of goat skin gelatin manufactured using weak base types of Ca(OH)2 and then compared with properties of commercial gelatin. Skins from Bligon goats of 1.5 to 2.5 years old was used as the raw materials and Ca(OH)2 100 g/l as curing materials. The 2x3 factorial completely randomized design (CRD) with three replications was used as a design study. Two curing times (2 and 4 days) and three concentrations (3, 6, 9% v/v) were used as treatments, and commercial gelatin (pure (P) by Merck, food grade (Fg) and pharmacy standards (Ps)) were used as control. Gelatin produced from goat skins using Ca(OH)2 had properties similar to that of commercial gelatin. The heavy metals (Pb, Cu and Zn) contained in goat skin gelatin still meet the INS standards. The optimum production of gelatin has been generated through the application of 4-day curing time at a concentration of 9% (v/v).

A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using BseDI restriction enzyme had been applied for identifying the presence of pork in processed meat (beef sausage and chicken nugget) including before and after frying. Pork sample in various levels (1%, 3%, 5%, 10%, and 25 %) was prepared in a mixture with beef and chicken meats and processed for sausage and nugget. The primers CYTb1 and CYTb2 were designed in the mitochondrial cytochrome b (cyt b ) gene and PCR successfully amplified fragments of 359 bp. To distinguish existence of porcine species, the amplified PCR products of mitochondrial DNA were cut by BseDI restriction enzyme. The result showed pig mitochondrial DNA was cut into 131 and 228 bp fragments. The PCR-RFLP species identification assay yielded excellent results for identification of porcine species. It is a potentially reliable technique for pork detection in animal food processed products for Halal authentication.

The objective of this study was to identify the Angiotensin Converting Enzyme (ACE) inhibitorypeptide derived from Kacang goat meat protein hydrolysate. Kacang goat meat loin section washydrolyzed with pepsin, trypsin and chymotrypsin. Protein hydrolysate of Kacang goat meat was thentested the protein concentration and ACE inhibitory activity. ACE inhibitory peptide of the proteinhydrolysate was purified through several steps of purification by column SEP-PAK Plus C18 Cartridgeand RP-HPLC using a Cosmosil column 5PE-SM, 4.6 x 250 mm. The sequence of amino acid of ACEinhibitory peptide was identified by amino acid sequencer. The results showed that amino acidssequence of ACE inhibitory peptide derived from protein hydrolysate of Kacang goat meat was leu-thrglu-ala-pro-leu-asn-pro-lys-ala-arg- asn-glu-lys. It had a molecular weight (MW) of 1581 and occurredat the position of 20th to 33rd residues of b-actin of goat meat protein (Capra hircus). The ACE inhibitoryactivity (IC50) of the peptide was 190 mg/mL or 120 mM.

Penelitian bertujuan untuk mengetahui pengaruh perbedaan proporsi filler tepung mocaf ( Modified Cassava Flour ) terhadap kualitas fisik, sensoris dan kadar kolesterol nugget ampela ayam. Penelitian dilakukan dengan menggunakan 5 level filler tepung mocaf dan ampela dengan imbangan 0 : 100; 5 : 95; 10 : 90; 15 : 85; dan 20 : 80. Data yang diambil adalah kualitas fisik (pH, keempukan, daya ikat air), kualitas sensoris (warna, rasa, tekstur, daya terima) dan kadar kolesterol. Data kualitas fisik dan kadar kolesterol dianalisis dengan analisis variansi Rancangan Acak Lengkap pola searah atau One Way Anova dan data kualitas sensoris dianalisis dengan analisis non -parametrik dengan uji Hedonik Kruskal Wallis . Perbedaan rerata diuji dengan uji Duncan’s New Multiple Range Test . Hasil penelitian menunjukkan bahwa perbedaan level filler tepung mocaf berpengaruh pada penurunan keempukan dan peningkatan daya ikat air tetapi tidak mempengaruhi nilai pH. Peningkatan level filler tepung mocaf meningkatkan skor tekstur tetapi tidak mempengaruhi warna, rasa dan daya terima. Peningkatan level filler tepung mocaf tidak berpengaruh terhadap penurunan kadar kolesterol nugget ampela. Kesimpulan dari penelitian adalah bahwa makin tinggi level filler tepung mocaf yang ditambahkan pada nugget ampela akan meningkatkan daya ikat air dan tekstur serta menurunkan keempukan, tetapi tidak merubah daya terima dan kadar kolesterol dari nugget ampela. (Kata kunci: Nugget ampela, Filler, Tepung mocaf, Kualitas fisik, Kualitas sensoris, Kadar kolesterol)

Penelitian ini dilakukan dengan tujuan untuk mengetahui kemampuan ensim microbial trans glutaminase dalam memperbaiki kualitas bakso daging layu dan untuk mengetahui konsentrasi ensim yang dibutuhkan. Transglutaminase merupakan ensim yang berfungsi untuk membuat ikatan silang antar protein melalui ikatan a-(g-Glutamyl) lysine sehingga mampu meningkatkan sifat fungsional dari protein. Pada proses pembuatan bakso daging layu ditambahkan ensim dengan konsentrasi 0 (kontrol), 0,01,0,05 dan 0,1% (berat/berat). Adonan bakso yang sudah mengandung ensim diinkubasi pada suhu 45°C selama 30 menit untuk memberi kesempatan aktivitas ensim bekerja dan dilanjutkan dengan perebusan sekitar 5 sampai 10 menit. Hasil penelitian menunjukkan tingkat kekenyalan bakso tidak berbeda antar daging segar dan daging layu, penambahan ensim dapat meningkatkan tingkat kekenyalan bakso. Level ensim yang menunjukkan tingkat kekenyalan tertinggi pada level 0,01%. Konfirmasi biopolimer ikatan silang antar protein daging dengan Sodium Dodecyl Sulphate-Poly-Acrylamide Gel Electrophoresis (SDS-PAGE) menunjukan band tebal sebagai tanda terbentuknya polimer pada konsentrasi ensim 0,05%. Kualitas organoleptik bakso diuji secara panelis meliputi warna, rasa, tekstur dan keempukan bakso. Hasil analisis menunjukkan bahwa nilai warna dan tekstur bakso tidak berbeda nyata (p>0,05) pada bakso daging segar, layu tanpa ensim dan layu dengan tambahan ensim. Untuk kualitas rasa dan keempukan para panelis memberikan penilaian yang berbeda (P

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Penelitian bertujuan memanfaatkan gelatin kulit kaki ayam yang dikombinasikan dengan soy protein isolate sebagai bahan baku edible film. Materi penelitian adalah gelatin kulit kaki ayam dan soy protein isolate. Penelitian terbagi atas dua tahap yaitu tahap pertama adalah pembuatan gelatin kulit kaki ayam yang direndam dengan NaOH 0,1%, asam sulfat 0,1%, dan asam sitrat 0,4%. Tahap kedua yaitu pembuatan edible film yang dikombinasikan dengan soy protein isolate pada perbandingan (75:25, 80:20, 85:15, 90:10, dan 95:5). Analisis data mengunakan variansi pola searah, apabila terdapat beda nyata diteruskan uji Duncan’s New Multiple Range Test. Profil protein dianalisis denganSDS-PAGE dan menunjukkan sebagai molekul kolagen. Hasil analisis kandungan asam amino edible film menggunakan HPLC dihasilkan komposisi residu asam amino terbesar adalah glysin yaitu 29,42%, 37,88%, 38,32%, 39,28% dan 39,17% pada masing-masing perlakuan. Hal itu menggambarkan bahwa profil protein edible film dapat dipastikan sebagian besar berasal dari kolagen gelatin. Pengamatan dengan scaning electron microscope menunjukkan telah terbentuk cross linking antara molekul protein gelatin dan molekul soy protein isolate dan yang ditunjukan semakin berkurangnya retakan seiring dengan meningkatnya konsentrasi gelatin. Perbedaan kombinasi gelatin kulit kaki ayam dan soy protein isolate untuk membentuk edible film tidak memberikan pengaruh nyata pada kekuatan tarik (tensile strenght), dan kemuluran (elongation), namun berpengaruh nyata pada laju transmisi uap air (Water Vapour Transmision Rate). Kombinasi 95:5 protein gelatin kulit kaki ayam dan soy protein isolate menghasilkan edible film yang terbaik.(Kata kunci: Edible film, Gelatin kaki ayam, Soy protein isolate)

The objective of the experiment study was to determine the effect of canary shell liquid smoke on the chemical, physical and sensory qualities of beef stored at room temperature. Beef samples were dipped in liquid smoke solutionwith the concentration of 0, 4, 8, and 12% (v/v) during 15 minutes and then stored for 0,2, and 4 days at room temperature. Each treatment was carried out three times and the variables measured were water, phenol and acidcontent, pH, water-holding capacity, cooking loss and sensory properties of samples. Data on chemical and physical qualities were analyzed using analysis of variance (Completely Randomized Design/CRD) with factorial 4x3. Thesensory properties were analyzed by a non parametric h-test. The results showed that liquid smoke concentration up to 12% significantly (psignificantly (pinteraction between liquid smoke concentration and storage time. In conclusion, the addition of liquid smoke up to 12% increased phenol and acid content, while the physical quality of meat has decreased. Quality of beef stored up to 4 daysshowed a decreased of quality such as pH, water-holding capacity, cooking loss, and sensory meat.(Keywords: Canary shell, Liquid smoke, Storage time, Quality of beef)