COMPARATIVE STUDY ON ANGIOTENSIN CONVERTING ENZYME INHIBITORY ACTIVITY OF HYDROLYSATE OF MEAT PROTEIN OF INDONESIAN LOCAL LIVESTOCKS

The experiment was conducted to investigate the angiotensin converting enzyme (ACE) inhibitory activity of hydrolysate in meat protein of Bali cattle, Kacang goat, native chicken, and local duck. The meats of Bali cattle, Kacang goat, native chicken, and local duck were used in this study. The meats were ground using food processor added with aquadest to obtain meat extract. The meat extracts were then hydrolyzed using protease enzymes to obtain hydrolysate of meat protein. Protein concentration of meat extract and hydrolysate of meat protein were determined, and were confirmed by sodium dodecyl sulfate - poly acrylamide gel electrophoresis (SDS-PAGE). ACE inhibitory activity of hydrolysate of meat protein derived from Bali cattle, Kacang goat, native chicken, and local duck was also determined. The results showed that protein concentration of hydrolysate of meat protein of Bali cattle, Kacang goat, native chicken, and local duck meat was significantly higher than their meat extracts. SDS-PAGE analysis indicated that hydrolysate of meat protein of Bali cattle, Kacang goat, native chicken, and local duck had more peptides with lower molecular weight, compared to their meat extracts. Hydrolysate of meat protein of Bali cattle, Kacang goat, native chicken, and local duck had potencies in inhibiting ACE activity, so it will potentially reduce blood pressure.