1. Transient CENP-E-like kinetochore proteins in plants.
- Author
-
ten Hoopen R, Schleker T, Manteuffel R, and Schubert I
- Subjects
- Amino Acid Sequence, Cell Nucleus metabolism, Centromere metabolism, Chromosomal Proteins, Non-Histone chemistry, Chromosomal Proteins, Non-Histone metabolism, Chromosomes metabolism, Conserved Sequence, Escherichia coli genetics, Hordeum genetics, Humans, Lymphocytes metabolism, Meristem cytology, Meristem metabolism, Mitosis, Molecular Sequence Data, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Sequence Homology, Amino Acid, Vicia genetics, Chromosomal Proteins, Non-Histone genetics, Kinetochores metabolism, Plant Proteins genetics
- Abstract
Derived from candidate sequences of a barley EST database two proteins with homology to the coiled coil region of the human kinetochore protein (KP) CENP-E were generated and classified as centromere protein E-like 1 and 2 (Cpell and Cpe12). Specific antibodies produced against recombinant Cpe11 and Cpe12 proteins labeled the centromere on mitotic chromosomes of barley and field bean and recognized specifically proteins from nuclear/chromosomal protein extracts on immunoblots. No function was predicted for homologues of Cpe11 within the databases for Arabidopsis and rice genomes. However, the centromeric location of Cpe11 and Cpe12 suggests they may have a function within the kinetochore. Plant homologues to barley Cpe12 are N-type kinesins, suggesting that Cpe12 is functionally homologous to human CENP-E.
- Published
- 2002
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