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Evolutionary conservation of kinetochore protein sequences in plants
- Source :
- Chromosoma. 109(7)
- Publication Year :
- 2001
-
Abstract
- The evolutionary conservation of structural/functional kinetochore proteins has been studied on isolated nuclei and pro-/metaphase chromosomes of mono- and dicot plants. The cross-reactivities of antibodies against human CENPC, CENPE and CENPF, and against maize CENPCa with the centromeric regions of mitotic chromosomes of Vicia faba and/or Hordeum vulgare are shown. Putative homologs of the kinetochore protein SKP1 (suppressor of kinetochore protein 1p of yeast) were found in both species and of CBF5p (centromere binding factor 5 of yeast) in barley. Antibodies against synthetic peptides derived from partial sequences encoding these proteins were produced and recognized the centromeric regions on mitotic chromosomes as detected by indirect immunofluorescence.
- Subjects :
- Genetics
DNA, Complementary
Base Sequence
Sequence Homology, Amino Acid
Kinetochore
Molecular Sequence Data
CENPF
food and beverages
Hordeum
Biology
Zea mays
Conserved sequence
Vicia faba
Evolution, Molecular
biology.protein
Homologous chromosome
Hordeum vulgare
Amino Acid Sequence
Kinetochores
Metaphase
Mitosis
Genetics (clinical)
DNA Primers
Plant Proteins
Subjects
Details
- ISSN :
- 00095915
- Volume :
- 109
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Chromosoma
- Accession number :
- edsair.doi.dedup.....ff7e3b4fb2c6cfdd9236e58b012df774