10 results on '"Wang, Beilei"'
Search Results
2. Application of fully-covered self-expandable metallic stent for treatment of pancreatic-biliary diseases
- Author
-
Zhang Li-ming, Wen Xiaojuan, Lu Jia, Xiao Liang, Zhang Wei, Wang Qianqian, Liu Guoyan, Zhao Jie, Zhang Lin, Wang Beilei, and Wang Tao
- Subjects
inorganic chemicals ,Jellyfish ,Erythrocyte suspension ,Tentacle ,biology ,General Medicine ,Cyanea (jellyfish) ,biology.organism_classification ,Molecular biology ,body regions ,Toxicology ,biology.animal ,Chelation ,Whole blood - Abstract
Objective To investigate the potential role of the pore-formation in the hemolytic activity of tentacle-only extract(TOE) from the jellyfish Cyanea capillata.Methods The effects of various cations,including K+,Ca2+,Mg2+,Mn2+,Zn2+,Cu2+,Fe2+,La3+and NH4+ on the hemolytic activity of TOE were compared in two different test systems: 1% whole blood and 0.45% erythrocyte suspension with the same erythrocyte concentration.Results The hemolytic activities of TOE in both tests were inhibited by Mn2+,Zn2+,La3+,Cu2+ and Fe2+,and were promoted to a minor extent by K+,Ca2+,Mg2+ and NH4+.The chelating agent EDTA also inhibited the hemolytic activity of TOE.Conclusion The pore-formation mechanism might play an important role in the hemolytic activity of TOE.
- Published
- 2013
3. A novel granulin homologue isolated from the jellyfish Cyanea capillata promotes proliferation and migration of human umbilical vein endothelial cells through the ERK1/2-signaling pathway.
- Author
-
Wang, Chao, Wang, Beilei, Wang, Bo, Wang, Qianqian, Liu, Guoyan, Fan, Chongxu, and Zhang, Liming
- Subjects
- *
UMBILICAL veins , *ENDOTHELIAL cells , *CELL cycle proteins , *JELLYFISHES , *PLANT growth promoting substances - Abstract
Jellyfish grow rapidly and have a strong regenerative ability, indicating that they may express high levels of growth factors. Therefore, the aim of this research was to isolate the growth-promoting components from the jellyfish Cyanea capillata (C. capillata) and to further explore the underlying mechanisms. In this study, we first isolated and identified a novel polypeptide from C. capillata tentacles using size-exclusion chromatography followed by reverse-phase HPLC. This peptide, consisting of 58 amino acids (MW 5782.9 Da), belonged to the granulin (GRN) family of growth factors; thus, we named it Cyanea capillata granulin-1 (Cc GRN-1). Second, using CCK-8 assay and flow cytometry, we verified that Cc GRN-1 at the 0.5 μg/ml concentration could promote cell proliferation and increase the expression of cell-cycle proteins (CyclinB1 and CyclinD1). Third, signaling pathways studies showed that Cc GRN-1 could activate the PI3K/Akt- and ERK1/2 MAPK–signaling pathways but not the JNK MAPK- or NF-κB-signaling pathways. Subsequently, we further confirmed that the Cc GRN-1-induced cell proliferation and migration were associated only with the ERK1/2 MAPK–signaling pathway. Considering all of these factors, Cc GRN-1, as the first jellyfish-derived GRN homologue, possesses growth-promoting properties and may be a candidate for novel therapeutics to promote human wound healing in unfavorable conditions. Unlabelled Image [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
4. Tentacle extract from the jellyfish Cyanea capillata increases proliferation and migration of human umbilical vein endothelial cells through the ERK1/2 signaling pathway.
- Author
-
Wang, Beilei, Liu, Dan, Wang, Chao, Wang, Qianqian, Zhang, Hui, Liu, Guoyan, He, Qian, and Zhang, Liming
- Subjects
- *
TENTACLES (Animal anatomy) , *LION'S mane jellyfish , *UMBILICAL veins , *ENDOTHELIAL cells , *CELL migration , *CELL proliferation , *CELLULAR signal transduction - Abstract
Wound healing is a complex biological process, and current research finds that jellyfish have a great capacity for promoting growth and healing. However, the underlying mechanisms remain unclear. Thus, this study was conducted to investigate the molecular mechanisms and effects of a tentacle extract (TE) from the jellyfish Cyanea capillata (C. capillata) on cell proliferation and migration in human umbilical vein endothelial cells (HUVECs). First, our results showed that TE at the concentration of 1 μg/ml could promote cell proliferation over various durations, induce a transition of the cells from the G1-phase to the S/G2-phase of the cell cycle, and increase the expression of cell cycle proteins (CyclinB1 and CyclinD1). Second, we found that TE could activate the PI3K/Akt, ERK1/2 and JNK MAPK signaling pathways but not the NF-κB signaling pathway or the apoptosis signaling cascade. Finally, we demonstrated that the TE-induced expression of cell cycle proteins was decreased by ERK1/2 inhibitor PD98059 but not by PI3K inhibitor LY294002 or JNK inhibitor SP600125. Similarly, the TE-enhanced migration ability of HUVECs was also markedly attenuated by PD98059. Taken together, our findings indicate that TE-induced proliferation and migration in HUVECs mainly occurred through the ERK1/2 MAPK signaling pathway. These results are instructively important for further research on the isolation and purification of growth-promoting factors from C. capillata and are hopeful as a means to improve human wound repair in unfavorable conditions. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
5. Protective effects of batimastat against hemorrhagic injuries in delayed jellyfish envenomation syndrome models.
- Author
-
Wang, Beilei, Liu, Dan, Liu, Guoyan, Zhang, Xin, Wang, Qianqian, Zheng, Jiemin, Zhou, Yonghong, He, Qian, and Zhang, Liming
- Subjects
- *
JELLYFISHES , *NEOVASCULARIZATION inhibitors , *METALLOPROTEINASES , *CEREBRAL hemorrhage , *EXTRACELLULAR matrix , *HISTOPATHOLOGY , *DISEASES - Abstract
Previously, we established delayed jellyfish envenomation syndrome (DJES) models and proposed that the hemorrhagic toxins in jellyfish tentacle extracts (TE) play a significant role in the liver and kidney injuries of the experimental model. Further, we also demonstrated that metalloproteinases are the central toxic components of the jellyfish Cyanea capillata ( C. capillata ), which may be responsible for the hemorrhagic effects. Thus, metalloproteinase inhibitors appear to be a promising therapeutic alternative for the treatment of hemorrhagic injuries in DJES. In this study, we examined the metalloproteinase activity of TE from the jellyfish C. capillata using zymography analyses. Our results confirmed that TE possessed a metalloproteinase activity, which was also sensitive to heat. Then, we tested the effect of metalloproteinase inhibitor batimastat (BB-94) on TE-induced hemorrhagic injuries in DJES models. Firstly, using SR-based X-ray microangiography, we found that BB-94 significantly improved TE-induced hepatic and renal microvasculature alterations in DJES mouse model. Secondly, under synchrotron radiation micro-computed tomography (SR-μCT), we also confirmed that BB-94 reduced TE-induced hepatic and renal microvasculature changes in DJES rat model. In addition, being consistent with the imaging results, histopathological and terminal deoxynucleotidyl transferase-mediated UTP end labeling (TUNEL)-like staining observations also clearly corroborated this hypothesis, as BB-94 was highly effective in neutralizing TE-induced extensive hemorrhage and necrosis in DJES rat model. Although it may require further clinical studies in the near future, the current study opens up the possibilities for the use of the metalloproteinase inhibitor, BB-94, in the treatment of multiple organ hemorrhagic injuries in DJES. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
6. Cardiovascular Effect Is Independent of Hemolytic Toxicity of Tentacle-Only Extract from the Jellyfish Cyanea capillata
- Author
-
Zhang Li-ming, Xiao Liang, Lu Jia, Wang Yang, Wang Qianqian, Li Ying, Liu Guoyan, Liu Sihua, Wang Beilei, and Ye Xuting
- Subjects
Male ,Jellyfish ,Anatomy and Physiology ,Critical Care and Emergency Medicine ,Scyphozoa ,medicine.medical_treatment ,lcsh:Medicine ,Venom ,Pharmacology ,Toxicology ,Cardiovascular ,Cardiovascular System ,Biochemistry ,Rats, Sprague-Dawley ,Myocytes, Cardiac ,lcsh:Science ,Multidisciplinary ,biology ,Chemistry ,Neurochemistry ,Hemolysis ,Cardiovascular physiology ,Blood Chemistry ,Toxicity ,Medicine ,Research Article ,Drugs and Devices ,Heart Ventricles ,Toxic Agents ,Marine Biology ,Cyaneidae ,Cardiovascular Pharmacology ,Cnidarian Venoms ,Microscopy, Electron, Transmission ,biology.animal ,Chemical Biology ,medicine ,Animals ,Animal Physiology ,Biology ,Dialysis ,Myocardium ,Acute Cardiovascular Problems ,lcsh:R ,Hemodynamics ,Proteins ,medicine.disease ,biology.organism_classification ,Rats ,lcsh:Q ,Cardiovascular Injury ,Blood Gas Analysis ,Zoology - Abstract
Our previous studies have confirmed that the crude tentacle-only extract (cTOE) from the jellyfish Cyanea capillata (Cyaneidae) exhibits hemolytic and cardiovascular toxicities simultaneously. So, it is quite difficult to discern the underlying active component responsible for heart injury caused by cTOE. The inactivation of the hemolytic toxicity from cTOE accompanied with a removal of plenty of precipitates would facilitate the separation of cardiovascular component and the investigation of its cardiovascular injury mechanism. In our research, after the treatment of one-step alkaline denaturation followed by twice dialysis, the protein concentration of the treated tentacle-only extract (tTOE) was about 1/3 of cTOE, and SDS-PAGE showed smaller numbers and lower density of protein bands in tTOE. The hemolytic toxicity of tTOE was completely lost while its cardiovascular toxicity was well retained. The observations of cardiac function, histopathology and ultrastructural pathology all support tTOE with significant cardiovascular toxicity. Blood gas indexes and electrolytes changed far less by tTOE than those by cTOE, though still with significant difference from normal. In summary, the cardiovascular toxicity of cTOE can exist independently of the hemolytic toxicity and tTOE can be employed as a better venom sample for further purification and mechanism research on the jellyfish cardiovascular toxic proteins.
- Published
- 2012
7. Detection of microvasculature alterations by synchrotron radiation in murine with delayed jellyfish envenomation syndrome.
- Author
-
Wang, Beilei, Zhang, Bo, Huo, Hua, Wang, Tao, Wang, Qianqian, Wu, Yuanlin, Xiao, Liang, Ren, Yuqi, and Zhang, Liming
- Subjects
- *
SYNCHROTRON radiation , *JELLYFISHES , *HISTOPATHOLOGY , *THREE-dimensional imaging , *BLOOD-vessel physiology , *LABORATORY mice - Abstract
Abstract: Using the tentacle extract (TE) from the jellyfish Cyanea capillata, we have previously established a delayed jellyfish envenomation syndrome (DJES) model, which is meaningful for clinical interventions against jellyfish stings. However, the mechanism of DJES still remains unclear. Thus, this study aimed to explore its potential mechanism by detecting TE-induced microvasculature alterations in vivo and ex vivo. Using a third-generation synchrotron radiation facility, we, for the first time, directly observed the blood vessel alterations induced by jellyfish venom in vivo and ex vivo. Firstly, microvasculature imaging of whole-body mouse in vivo indicated that the small blood vessel branches in the liver and kidney in the TE-treated group, seemed much thinner than those in the control group. Secondly, 3D imaging of kidney ex vivo showed that the kidneys in the TE-treated group had incomplete vascular trees where distal vessel branches were partly missing and disorderly disturbed. Finally, histopathological analysis found that obvious morphological changes, especially hemorrhagic effects, were also present in the TE-treated kidney. Thus, TE-induced microvasculature changes might be one of the important mechanisms of multiple organ dysfunctions in DJES. In addition, the methods we employed here will probably facilitate further studies on developing effective intervention strategies against DJES. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
8. Multiple organ dysfunction: A delayed envenomation syndrome caused by tentacle extract from the jellyfish Cyanea capillata
- Author
-
Wang, Beilei, Zhang, Lin, Zheng, Jiemin, Wang, Qianqian, Wang, Tao, Lu, Jia, Wen, Xiaojuan, Zhang, Bo, Liu, Guoyan, Zhang, Wei, Xiao, Liang, and Zhang, Liming
- Subjects
- *
MULTIPLE organ failure , *JELLYFISHES , *VENOM , *CLINICAL chemistry , *IMMUNOHISTOCHEMISTRY , *KIDNEY injuries , *CELL-mediated cytotoxicity , *VASOCONSTRICTION - Abstract
Abstract: The delayed jellyfish envenomation syndrome (DJES) with serious multiple organ dysfunction or systemic damages, generally developed 2 h after jellyfish stings, deserves special attention for it is very meaningful to the clinical interventions. To set up a DJES model as well as to obtain more details about its process, an integrative approach, including clinical chemistry, pathology and immunohistochemistry, was conducted to simultaneously monitor the effects of tentacle extract (TE) from the jellyfish Cyanea capillata on the vital target organs (heart, lung, liver and kidney). Our results showed that the TE from C. capillata could induce diverse toxic effects on these organs, among which the liver and kidney injuries seemed to be more serious than cardiopulmonary injuries and might be the leading causes of death in rats with DJES. In summary, we have established a DJES model with multiple organ dysfunction, which could facilitate the research on its underlying mechanism as well as the development of specific prevention or therapy strategies against jellyfish envenomation. The application of this model suggested that the possible mechanism of DJES might be attributed to the synergy of cytotoxicity, vasoconstriction effect and other specific target organ toxicities of jellyfish venom. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
9. Molecular cloning and functional characterization of a Cu/Zn superoxide dismutase from jellyfish Cyanea capillata.
- Author
-
Wang, Bo, Liu, Guoyan, Wang, Chao, Ruan, Zengliang, Wang, Qianqian, Wang, Beilei, Qiu, Leilei, Zou, Shuaijun, Zhang, Xiping, and Zhang, Liming
- Subjects
- *
MOLECULAR cloning , *SUPEROXIDE dismutase , *ANTISENSE DNA , *JELLYFISHES , *RECOMBINANT proteins , *COLUMN chromatography , *SUPEROXIDES - Abstract
We identified and characterized a novel superoxide dismutase (SOD), designated as CcSOD1, from the cDNA library from the tentacle tissue of the jellyfish Cyanea capillata. The full-length cDNA sequence of CcSOD1 consists of 745 nucleotides with an open reading frame encoding a mature protein of 154 amino acids, sharing a predicted structure similar to the typical Cu/Zn-SODs. The CcSOD1 coding sequence was cloned into the expression vector pET-24a and successfully expressed in Escherichia coli Rosetta (DE3) pLysS. The recombinant protein rCcSOD1 was purified by HisTrap High Performance chelating column chromatography and analyzed for its biological function. Our results showed that the purified rCcSOD1 could inhibit superoxide anion and keep active in a pH interval of 4.5–9 and a temperature interval of 10–70°C. Even when heated at 70°C for 60 min, rCcSOD1 retained 100% activity, indicating a relatively high thermostability. These results suggest that CcSOD1 protein may play an important role in protecting jellyfish from oxidative damage and can serve as a new resource for antioxidant products. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
10. Mitochondrial dysfunction contributes to the cytotoxicity induced by tentacle extract from the jellyfish Cyanea capillata in rat renal tubular epithelial NRK-52E cells.
- Author
-
Wang, Tao, He, Qian, Xiao, Liang, Wang, Qianqian, Zhang, Bo, Wang, Beilei, Liu, Guoyan, Zheng, Jiemin, Yu, Bentong, and Zhang, Liming
- Subjects
- *
MITOCHONDRIAL pathology , *CELL-mediated cytotoxicity , *ANIMAL extracts , *JELLYFISHES , *LION'S mane jellyfish , *EPITHELIAL cells , *LABORATORY rats - Abstract
Abstract: Our previous studies have shown that tentacle extract (TE) from the jellyfish Cyanea capillata could induce a delayed jellyfish envenomation syndrome with severe multiple organ dysfunctions, among which renal injury with tubular necrosis seemed to be most serious. So, in this study, we aimed to explore the toxic effect of TE on rat renal tubular epithelial NRK-52E cells. Based on the previous findings that TE could cause oxidative damage in erythrocytes, the effects of TE on cell oxidative stress conditions, including ROS production and lipid peroxidation, and mitochondrial dysfunction associated with cell death were investigated in NRK-52E cells. The results showed that TE caused cell morphological change and decreased cell viability through induction of apoptosis and necrosis in NRK-52E cells. Meanwhile, ROS overproduction and mitochondrial membrane potential decrease were found before the cell death occurred. It was concluded that TE could induce cytotoxicity, especially apoptosis and necrosis, in NRK-52E cells, and mitochondrial dysfunction and ROS overproduction might play important roles in the process of cell injury and death. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.