Back to Search
Start Over
Cardiovascular Effect Is Independent of Hemolytic Toxicity of Tentacle-Only Extract from the Jellyfish Cyanea capillata
- Source :
- PLoS ONE, PLoS ONE, Vol 7, Iss 8, p e43096 (2012)
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- Our previous studies have confirmed that the crude tentacle-only extract (cTOE) from the jellyfish Cyanea capillata (Cyaneidae) exhibits hemolytic and cardiovascular toxicities simultaneously. So, it is quite difficult to discern the underlying active component responsible for heart injury caused by cTOE. The inactivation of the hemolytic toxicity from cTOE accompanied with a removal of plenty of precipitates would facilitate the separation of cardiovascular component and the investigation of its cardiovascular injury mechanism. In our research, after the treatment of one-step alkaline denaturation followed by twice dialysis, the protein concentration of the treated tentacle-only extract (tTOE) was about 1/3 of cTOE, and SDS-PAGE showed smaller numbers and lower density of protein bands in tTOE. The hemolytic toxicity of tTOE was completely lost while its cardiovascular toxicity was well retained. The observations of cardiac function, histopathology and ultrastructural pathology all support tTOE with significant cardiovascular toxicity. Blood gas indexes and electrolytes changed far less by tTOE than those by cTOE, though still with significant difference from normal. In summary, the cardiovascular toxicity of cTOE can exist independently of the hemolytic toxicity and tTOE can be employed as a better venom sample for further purification and mechanism research on the jellyfish cardiovascular toxic proteins.
- Subjects :
- Male
Jellyfish
Anatomy and Physiology
Critical Care and Emergency Medicine
Scyphozoa
medicine.medical_treatment
lcsh:Medicine
Venom
Pharmacology
Toxicology
Cardiovascular
Cardiovascular System
Biochemistry
Rats, Sprague-Dawley
Myocytes, Cardiac
lcsh:Science
Multidisciplinary
biology
Chemistry
Neurochemistry
Hemolysis
Cardiovascular physiology
Blood Chemistry
Toxicity
Medicine
Research Article
Drugs and Devices
Heart Ventricles
Toxic Agents
Marine Biology
Cyaneidae
Cardiovascular Pharmacology
Cnidarian Venoms
Microscopy, Electron, Transmission
biology.animal
Chemical Biology
medicine
Animals
Animal Physiology
Biology
Dialysis
Myocardium
Acute Cardiovascular Problems
lcsh:R
Hemodynamics
Proteins
medicine.disease
biology.organism_classification
Rats
lcsh:Q
Cardiovascular Injury
Blood Gas Analysis
Zoology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 7
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....c3b297b8215c4ff54fdc3bf56432ba6b