1. Effect of Ischemic Postconditioning and Atorvastatin in the Prevention of Remote Lung Reperfusion Injury.
- Author
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Santos CHMD, Dourado DM, Silva BAKD, Pontes HBD, Azevedo Neto E, Vendas GSDC, Chaves IO, Miranda JVC, Oliva JVDG, Dias LDES, Almeida MHM, and Sampaio TL
- Subjects
- Animals, Aorta, Abdominal, Combined Modality Therapy, Ischemia pathology, Ischemia prevention & control, Lung pathology, Male, Rats, Wistar, Reperfusion Injury pathology, Reproducibility of Results, Time Factors, Treatment Outcome, Atorvastatin therapeutic use, Hydroxymethylglutaryl-CoA Reductase Inhibitors therapeutic use, Ischemic Postconditioning methods, Lung blood supply, Reperfusion Injury prevention & control
- Abstract
Objective: The aim of the present study was to evaluate the ability of ischemic postconditioning, atorvastatin and both associated to prevent or minimize reperfusion injury in the lung of rats subjected to ischemia and reperfusion by abdominal aortic clamping., Methods: We used 41 Wistar norvegic rats, which were distributed into 5 groups: ischemia and reperfusion (I/R), ischemic postcondictioning (IPC), postconditioning + atorvastatin (IPC+A), atorvastatin (A) and SHAM. It was performed a medium laparotomy, dissection and isolation of the infra-renal abdominal aorta; except for the SHAM group, all the others were submitted to the aortic clamping for 70 minutes (ischemia) and posterior clamp removal (reperfusion, 70 minutes). In the IPC and IPC+A groups, postconditioning was performed between the ischemia and reperfusion phases by four cycles of reperfusion and ischemia lasting 30 seconds each. In the IPC+A and A groups, preceding the surgical procedure, administration of 3.4 mg/day of atorvastatin was performed for seven days by gavage. After the surgical procedure, the right caudal lobe was removed from the lung for histological study, using tissue injury score ranging from grade 1 (normal tissue) to grade 4 (intense lesion)., Results: The mean lung injury was 3.6 in the I/R group, 1.6 in the IPC group, 1.2 in the IPC+A group, 1.2 in the A group, and 1 in the SHAM group (P<0.01)., Conclusion: Ischemic postconditioning and atorvastatin were able to minimize lung reperfusion injury, alone or in combination.
- Published
- 2018
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