1. IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic Fever.
- Author
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Flores-Mendoza LK, Estrada-Jiménez T, Sedeño-Monge V, Moreno M, Manjarrez MDC, González-Ochoa G, Millán-Pérez Peña L, and Reyes-Leyva J
- Subjects
- Animals, Biomarkers metabolism, Female, Flow Cytometry, Humans, Interferon-gamma metabolism, Interleukin-2 metabolism, Interleukin-4 metabolism, Interleukin-6 metabolism, Male, Suppressor of Cytokine Signaling 1 Protein metabolism, Tumor Necrosis Factor-alpha metabolism, Interleukin-10 metabolism, Severe Dengue metabolism, Suppressor of Cytokine Signaling 3 Protein metabolism
- Abstract
Background: Cytokines play important roles in the physiopathology of dengue infection; therefore, the suppressors of cytokine signaling ( socs ) that control the type and timing of cytokine functions could be involved in the origin of immune alterations in dengue., Objective: To explore the association of cytokine and socs levels with disease severity in dengue patients., Methods: Blood samples of 48 patients with confirmed dengue infection were analyzed. Amounts of interleukins IL-2, IL-4, IL-6, and IL-10, interferon- (IFN-) γ , and tumor necrosis factor- (TNF-) α were quantified by flow cytometry, and the relative expression of socs1 and socs3 mRNA was quantified by real-time RT-PCR., Results: Increased levels of IL-10 and socs3 and lower expression of socs1 were found in patients with dengue hemorrhagic fever (DHF) with respect to those with dengue fever (DF) ( p < 0.05). Negative correlations were found between socs1 and both IL-10 and socs3 ( p < 0.01). The cutoff values of socs3 (>199.8-fold), socs1 (<1.94-fold), and IL-10 (>134 pg/ml) have the highest sensitivity and specificity to discriminate between DF and DHF., Conclusion: Simultaneous changes in IL-10 and socs1/socs3 could be used as prognostic biomarkers of dengue severity.
- Published
- 2017
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