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IL-10 and socs3 Are Predictive Biomarkers of Dengue Hemorrhagic Fever.

Authors :: Flores-Mendoza LK
Estrada-Jiménez T
Sedeño-Monge V
Moreno M
Manjarrez MDC
González-Ochoa G
Millán-Pérez Peña L
Reyes-Leyva J
Source :: Mediators of inflammation [Mediators Inflamm] 2017; Vol. 2017, pp. 5197592. Date of Electronic Publication: 2017 Jul 30.
Publication Year :: 2017
Abstract: Background: Cytokines play important roles in the physiopathology of dengue infection; therefore, the suppressors of cytokine signaling ( socs ) that control the type and timing of cytokine functions could be involved in the origin of immune alterations in dengue.<br />Objective: To explore the association of cytokine and socs levels with disease severity in dengue patients.<br />Methods: Blood samples of 48 patients with confirmed dengue infection were analyzed. Amounts of interleukins IL-2, IL-4, IL-6, and IL-10, interferon- (IFN-) γ , and tumor necrosis factor- (TNF-) α were quantified by flow cytometry, and the relative expression of socs1 and socs3 mRNA was quantified by real-time RT-PCR.<br />Results: Increased levels of IL-10 and socs3 and lower expression of socs1 were found in patients with dengue hemorrhagic fever (DHF) with respect to those with dengue fever (DF) ( p < 0.05). Negative correlations were found between socs1 and both IL-10 and socs3 ( p < 0.01). The cutoff values of socs3 (>199.8-fold), socs1 (<1.94-fold), and IL-10 (>134 pg/ml) have the highest sensitivity and specificity to discriminate between DF and DHF.<br />Conclusion: Simultaneous changes in IL-10 and socs1/socs3 could be used as prognostic biomarkers of dengue severity.