1. Galuteolin suppresses proliferation and inflammation in TNF-α-induced RA-FLS cells by activating HMOX1 to regulate IKKβ/NF-κB pathway.
- Author
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Guan Y, Zhao X, Liu W, and Wang Y
- Subjects
- Anti-Inflammatory Agents, Apoptosis drug effects, Apoptosis genetics, Arthritis, Rheumatoid genetics, Arthritis, Rheumatoid pathology, Cells, Cultured, Dose-Response Relationship, Drug, Humans, Inflammation metabolism, Phytotherapy, Plant Extracts isolation & purification, Plant Extracts therapeutic use, Arthritis, Rheumatoid drug therapy, Arthritis, Rheumatoid etiology, Cell Proliferation drug effects, Heme Oxygenase-1 metabolism, I-kappa B Kinase metabolism, Inflammation prevention & control, Lonicera chemistry, NF-kappa B metabolism, Plant Extracts pharmacology, Signal Transduction drug effects, Signal Transduction genetics, Synoviocytes physiology, Tumor Necrosis Factor-alpha adverse effects
- Abstract
Objective: Galuteolin (Galu) is a substance extracted and purified from honeysuckle. The purpose of this study was to explore the effects of Galu on the TNF-α-induced RA-FLS cells (synoviocytes) and reveal its potential molecular mechanism from the perspectives of anti-apoptosis and anti-inflammation., Methods: After TNF-α stimulation, cell proliferation of RA-FLS was assessed by CCK-8 assay. TUNEL staining was used to detect the apoptosis. Western blot was used to detect the expressions of Iκκβ, p-p65, p65, p-IκB, IκB, Cleaved-caspase3, Caspase-3, Bcl-2, and Bax. HO-1 were determined by RT-PCR. The contents of pro-inflammatory cytokines IL-1β, IL-6, IL-8, and MMP-1 were determined by ELISA., Results: Galu significantly suppressed cell proliferation in a dose-dependent manner. Additionally, Galu obviously promotes cell apoptosis rate of RA-FLS cells and elevated the expression levels of HO-1, caspase-3, and Bax, while reducing the expression level of Bcl-2. Furthermore, Galu apparently inhibited the levels of Iκκβ, p-p65, and p-IκB. Moreover, Galu also significantly reduced the levels of pro-inflammatory factors IL-1β, IL-6, IL-8, and MMP-1 in RA-FLS cells., Conclusion: Galuteolin exerts protective effects against TNF-α-induced RA-FLS cells by inhibiting apoptosis and inflammation, which can guide the clinical use of rheumatoid arthritis.
- Published
- 2020
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