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Galuteolin suppresses proliferation and inflammation in TNF-α-induced RA-FLS cells by activating HMOX1 to regulate IKKβ/NF-κB pathway.
- Source :
-
Journal of orthopaedic surgery and research [J Orthop Surg Res] 2020 Oct 21; Vol. 15 (1), pp. 484. Date of Electronic Publication: 2020 Oct 21. - Publication Year :
- 2020
-
Abstract
- Objective: Galuteolin (Galu) is a substance extracted and purified from honeysuckle. The purpose of this study was to explore the effects of Galu on the TNF-α-induced RA-FLS cells (synoviocytes) and reveal its potential molecular mechanism from the perspectives of anti-apoptosis and anti-inflammation.<br />Methods: After TNF-α stimulation, cell proliferation of RA-FLS was assessed by CCK-8 assay. TUNEL staining was used to detect the apoptosis. Western blot was used to detect the expressions of Iκκβ, p-p65, p65, p-IκB, IκB, Cleaved-caspase3, Caspase-3, Bcl-2, and Bax. HO-1 were determined by RT-PCR. The contents of pro-inflammatory cytokines IL-1β, IL-6, IL-8, and MMP-1 were determined by ELISA.<br />Results: Galu significantly suppressed cell proliferation in a dose-dependent manner. Additionally, Galu obviously promotes cell apoptosis rate of RA-FLS cells and elevated the expression levels of HO-1, caspase-3, and Bax, while reducing the expression level of Bcl-2. Furthermore, Galu apparently inhibited the levels of Iκκβ, p-p65, and p-IκB. Moreover, Galu also significantly reduced the levels of pro-inflammatory factors IL-1β, IL-6, IL-8, and MMP-1 in RA-FLS cells.<br />Conclusion: Galuteolin exerts protective effects against TNF-α-induced RA-FLS cells by inhibiting apoptosis and inflammation, which can guide the clinical use of rheumatoid arthritis.
- Subjects :
- Anti-Inflammatory Agents
Apoptosis drug effects
Apoptosis genetics
Arthritis, Rheumatoid genetics
Arthritis, Rheumatoid pathology
Cells, Cultured
Dose-Response Relationship, Drug
Humans
Inflammation metabolism
Phytotherapy
Plant Extracts isolation & purification
Plant Extracts therapeutic use
Arthritis, Rheumatoid drug therapy
Arthritis, Rheumatoid etiology
Cell Proliferation drug effects
Heme Oxygenase-1 metabolism
I-kappa B Kinase metabolism
Inflammation prevention & control
Lonicera chemistry
NF-kappa B metabolism
Plant Extracts pharmacology
Signal Transduction drug effects
Signal Transduction genetics
Synoviocytes physiology
Tumor Necrosis Factor-alpha adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1749-799X
- Volume :
- 15
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of orthopaedic surgery and research
- Publication Type :
- Academic Journal
- Accession number :
- 33087158
- Full Text :
- https://doi.org/10.1186/s13018-020-02004-x