1. Co-Expression of Chicken IL-2 and IL-7 Enhances the Immunogenicity and Protective Efficacy of a VP2-Expressing DNA Vaccine against IBDV in Chickens.
- Author
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Huo S, Zhang J, Fan J, Wang X, Wu F, Zuo Y, and Zhong F
- Subjects
- Animals, Antibodies, Neutralizing, Antibodies, Viral immunology, Cell Line, Chickens, Host-Pathogen Interactions genetics, Host-Pathogen Interactions immunology, Humans, Immunity, Cellular, Infectious bursal disease virus genetics, Poultry Diseases genetics, Poultry Diseases mortality, Poultry Diseases prevention & control, Vaccines, DNA administration & dosage, Viral Structural Proteins genetics, Birnaviridae Infections veterinary, Gene Expression, Immunogenicity, Vaccine, Infectious bursal disease virus immunology, Interleukin-2 genetics, Interleukin-7 genetics, Vaccines, DNA immunology, Viral Structural Proteins immunology
- Abstract
Chicken infectious bursal disease (IBD) is still incompletely controlled worldwide. Although IBD virus (IBDV) VP2 DNA vaccine was considered a safe vaccine for IBD prevention, the immunogenicity by itself remains poor, resulting in the failure of effectively protecting chickens from infection. We and others demonstrated that chicken IL-2 (chIL-2) and chIL-7 have the capacity to enhance the immunogenicity of the VP2 DNA vaccine. However, whether chIL-2 and chIL-7 can mutually enhance the immunogenicity of VP2 DNA vaccine and thereby augment the latter's protection efficacy remains unknown. By using chIL-2/chIL-7 bicistronic gene vector to co-immunize the chickens together with the VP2 DNA vaccine, we now show that chIL-2 and chIL-7 significantly increased IBDV VP2-specific antibody titers, T cell proliferation, and IFN-γ production, resulting in the ultimate enhancement of vaccine-induced protection efficacy relative to that of chIL-2 or chIL-7 gene vectors alone. These results suggest that chIL-2 and chIL-7 can mutually enhance VP2 DNA vaccine's efficacy, thereby establishing a concrete foundation for future optimization of IBDV VP2 DNA vaccine to prevent/treat chicken IBD.
- Published
- 2019
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