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Co-Expression of Chicken IL-2 and IL-7 Enhances the Immunogenicity and Protective Efficacy of a VP2-Expressing DNA Vaccine against IBDV in Chickens.
- Source :
-
Viruses [Viruses] 2019 May 24; Vol. 11 (5). Date of Electronic Publication: 2019 May 24. - Publication Year :
- 2019
-
Abstract
- Chicken infectious bursal disease (IBD) is still incompletely controlled worldwide. Although IBD virus (IBDV) VP2 DNA vaccine was considered a safe vaccine for IBD prevention, the immunogenicity by itself remains poor, resulting in the failure of effectively protecting chickens from infection. We and others demonstrated that chicken IL-2 (chIL-2) and chIL-7 have the capacity to enhance the immunogenicity of the VP2 DNA vaccine. However, whether chIL-2 and chIL-7 can mutually enhance the immunogenicity of VP2 DNA vaccine and thereby augment the latter's protection efficacy remains unknown. By using chIL-2/chIL-7 bicistronic gene vector to co-immunize the chickens together with the VP2 DNA vaccine, we now show that chIL-2 and chIL-7 significantly increased IBDV VP2-specific antibody titers, T cell proliferation, and IFN-γ production, resulting in the ultimate enhancement of vaccine-induced protection efficacy relative to that of chIL-2 or chIL-7 gene vectors alone. These results suggest that chIL-2 and chIL-7 can mutually enhance VP2 DNA vaccine's efficacy, thereby establishing a concrete foundation for future optimization of IBDV VP2 DNA vaccine to prevent/treat chicken IBD.
- Subjects :
- Animals
Antibodies, Neutralizing
Antibodies, Viral immunology
Cell Line
Chickens
Host-Pathogen Interactions genetics
Host-Pathogen Interactions immunology
Humans
Immunity, Cellular
Infectious bursal disease virus genetics
Poultry Diseases genetics
Poultry Diseases mortality
Poultry Diseases prevention & control
Vaccines, DNA administration & dosage
Viral Structural Proteins genetics
Birnaviridae Infections veterinary
Gene Expression
Immunogenicity, Vaccine
Infectious bursal disease virus immunology
Interleukin-2 genetics
Interleukin-7 genetics
Vaccines, DNA immunology
Viral Structural Proteins immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1999-4915
- Volume :
- 11
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Viruses
- Publication Type :
- Academic Journal
- Accession number :
- 31137731
- Full Text :
- https://doi.org/10.3390/v11050476