1. Genetic Polymorphism in TNF-α-308 G/A and TNF-β +252 A/G, as Prognostic Biomarker in Breast Cancer Patients among Indian Population
- Author
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Jamshaid A. Siddiqui, Naseem Akhter, Nootan Kumar Shukla, Syed Akhtar Husain, Farah Parveen, and Mohammad Margoob Ahmad
- Subjects
Adult ,0301 basic medicine ,medicine.medical_specialty ,Genotype ,medicine.medical_treatment ,India ,Breast Neoplasms ,Immune responses ,Polymorphism, Single Nucleotide ,Gastroenterology ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Asian People ,Internal medicine ,Allele-specific oligonucleotide ,Biomarkers, Tumor ,Humans ,Medicine ,Genetic Predisposition to Disease ,Allele ,Promoter Regions, Genetic ,Lymphotoxin-alpha ,Cytokine ,Aged ,Aged, 80 and over ,Tumor Necrosis Factor-alpha ,business.industry ,Single nucleotide polymorphisms (SNPs) ,General Medicine ,Odds ratio ,Middle Aged ,Prognosis ,medicine.disease ,030104 developmental biology ,Tumor progression ,Case-Control Studies ,030220 oncology & carcinogenesis ,Female ,Tumour Necrosis Factor (TNF) ,Tumor necrosis factor alpha ,business ,Follow-Up Studies ,Research Article - Abstract
Background: Cytokines are the key regulator molecules that modulate immune response. Tumor necrosis factor (TNF- α-308 G/A and TNF-β +252 A/G ) are inflammatory cytokine that control the progression of several types of cancer. They play a vital role in both tumor progression and destruction based on their concentrations. The role of TNF-α-308 G/A and TNF-β +252 A/G gene polymorphism in the etiology of breast cancer (BC) is not clearly understood. Therefore, present study investigates the association of TNF-α -308 G/A and TNF-β +252 A/G and the clinical features with Breast cancer patients. Methods: In a case- control study, we have investigated 150 breast cancer patients and 300 age and ethnically matched healthy controls for duration of 3 years from North India. Promoter polymorphisms of tumor necrosis factor gene (TNF-α -308 G/A and TNF-β +252 A/G) were genotyped using allele specific oligonucleotide polymerase chain reaction ASO and restriction fragment length polymorphism (PCR-RFLP). The associations were evaluated by calculating the pooled odds ratio (OR) with 95% confidence interval (95% CI) using SPSS. Results: Patients with different clinico-pathological variables and healthy controls were analyzed. Significant association was observed in A allele of TNF-α -308 G/A in breast cancer patients as compared to healthy controls (p
- Published
- 2020