1. The role of lncRNA ANRIL in the progression of hepatocellular carcinoma
- Author
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Yongqiang Ma, Hong Hong, Weijie Han, Liansheng Zheng, Boshi Yan, Xiaolong Li, Dinghua Zhou, and Qiuhong Wang
- Subjects
Carcinoma, Hepatocellular ,Cell Survival ,Pharmaceutical Science ,Apoptosis ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Humans ,Gene silencing ,MTT assay ,Gene Silencing ,Wnt Signaling Pathway ,Cell Proliferation ,030304 developmental biology ,Pharmacology ,0303 health sciences ,Gene knockdown ,Sequence Analysis, RNA ,Cell growth ,Liver Neoplasms ,Wnt signaling pathway ,Hep G2 Cells ,Transfection ,Genes, bcl-2 ,Gene Expression Regulation, Neoplastic ,Lipofectamine ,Gene Knockdown Techniques ,030220 oncology & carcinogenesis ,Disease Progression ,Cancer research ,RNA, Long Noncoding ,bcl-Associated Death Protein ,Cell Migration Assays - Abstract
Objectives The aim of the current study was to identify the long noncoding RNAs (lncRNAs) ANRIL function and molecular pathways underlying hepatocellular carcinoma progression. Methods ANRIL knockdown with specific siRNA, and transfected into HepG2 cells according to the protocol of Lipofectamine 2000. Cell proliferation, apoptosis, migration and metastasis were assessed with MTT assay, flow cytometry and wound healing assay, respectively. Moreover, the expression level of ANRIL, apoptosis-related genes, and the Wnt pathway-associated genes were assessed by real time-PCR and Western blot assay. Key findings Knocking down of ANRIL led to alleviated cell growth and increased cell apoptosis of HepG2 cells through markedly increased expression levels of Bax and Bad. In contrast, dramatically diminished the expressions of anti-apoptotic factors including Bid and Bcl-2 in comparison to the scrambled control group (si-NC). Furthermore, ANRIL silencing resulted in an inactivated Wnt/β-catenin pathway by suppressing key genes associated with this pathway. Conclusions Taken together, these findings imply new insights into the regulatory network of the Wnt pathway through lncRNA ANRIL that indicate ANRIL may be a therapeutic factor potential for hepatocellular carcinoma.
- Published
- 2021
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