Back to Search
Start Over
LncRNA testis-specific transcript, Y-linked 15 (TTTY15) promotes proliferation, migration and invasion of colorectal cancer cells via regulating miR-29a-3p/DVL3 axis
LncRNA testis-specific transcript, Y-linked 15 (TTTY15) promotes proliferation, migration and invasion of colorectal cancer cells via regulating miR-29a-3p/DVL3 axis
- Source :
- Cancer Biomarkers. 31:1-11
- Publication Year :
- 2021
- Publisher :
- IOS Press, 2021.
-
Abstract
- BACKGROUND: Long non-coding RNA testis-specific transcript, Y-linked 15 (TTTY15) is oncogenic in prostate cancer, however its expression and function in colorectal cancer remain largely unknown. METHODS: Paired colorectal cancer samples/normal tissues were collected, and the expression levels of TTTY15, miR-29a-3p and disheveled segment polarity protein 3 (DVL3) were examined by quantitative real-time polymerase chain reaction (qRT-PCR); TTTY15 shRNA and overexpression plasmids were transfected into HT29 and HCT-116 cell lines using lipofectamine reagent, respectively; the proliferation and colony formation were detected by CCK-8 assay and plate colony formation assay; qRT-PCR and Western blot were used to analyze the changes of miR-29a-3p and DVL3; dual-luciferase reporter gene assay was used to determine the regulatory relationships between miR-29a-3p and TTTY15, miR-29a-3p and DVL3. RESULTS: TTTY15 was significantly up-regulated in cancerous tissues of colorectal cancer samples, positively correlated with the expression of DVL3, while negatively correlated with the expression of miR-29a-3p. After TTTY15 shRNAs were transfected into colorectal cancer cells, the proliferation and metastasis of cancer cells were significantly inhibited, while TTTY15 overexpression had opposite biological effects. TTTY15 shRNA could reduce the expression of DVL3 on both mRNA and protein levels, and the luciferase activity of TTTY15 sequence was also inhibited by miR-29a-3p. DVL3 was also validated as a target gene of miR-29a-3p, and it could be repressed by miR-29a-3p mimics or TTTY15 shRNA. CONCLUSION: TTTY15 is abnormally upregulated in colorectal cancer tissues, and it can modulate the proliferation and metastasis of colorectal cancer cells. It functions as the ceRNA to regulate the expression of DVL3 by sponging miR-29a-3p.
- Subjects :
- Male
Cancer Research
Colorectal cancer
Dishevelled Proteins
Biology
Transfection
Metastasis
Small hairpin RNA
03 medical and health sciences
0302 clinical medicine
Cell Movement
Genetics
medicine
Humans
Neoplasm Invasiveness
Cell Proliferation
030304 developmental biology
0303 health sciences
Reporter gene
Messenger RNA
General Medicine
Middle Aged
medicine.disease
MicroRNAs
Oncology
Lipofectamine
030220 oncology & carcinogenesis
Cancer cell
Cancer research
RNA, Long Noncoding
Colorectal Neoplasms
Subjects
Details
- ISSN :
- 18758592 and 15740153
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- Cancer Biomarkers
- Accession number :
- edsair.doi.dedup.....0dd1ba84a544fd21a78aca57173046d9
- Full Text :
- https://doi.org/10.3233/cbm-201709