1. Exosomal microRNA‐1246 from human umbilical cord mesenchymal stem cells potentiates myocardial angiogenesis in chronic heart failure
- Author
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Haiyan Lin, Weifeng Xu, Shengjie Wang, Zicheng Wang, Da Gao, and Yanwei Wang
- Subjects
Male ,Angiogenesis ,Myocardial Infarction ,Apoptosis ,Exosomes ,Exosome ,Umbilical cord ,Umbilical Cord ,Rats, Sprague-Dawley ,microRNA ,medicine ,Animals ,Humans ,Heart Failure ,Tube formation ,Exosome Multienzyme Ribonuclease Complex ,Neovascularization, Pathologic ,business.industry ,Myocardium ,Serine Endopeptidases ,Mesenchymal stem cell ,Heart ,Mesenchymal Stem Cells ,Cell Biology ,General Medicine ,Microvesicles ,Rats ,Disease Models, Animal ,MicroRNAs ,medicine.anatomical_structure ,cardiovascular system ,Cancer research ,Cord Blood Stem Cell Transplantation ,business ,Signal Transduction - Abstract
MicroRNAs (miRNAs) are of importance to chronic heart failure (CHF). However, the relevance of the exosomal miRNAs produced during CHF remains unknown. Our purpose here was to examine the relevance of exosomal miR-1246 released from human umbilical cord mesenchymal stem cell (hucMSC) during CHF and the mechanism of action. Cardiac function, myocardial infarction area, apoptosis, and angiogenesis were all evaluated in a CHF rat model following treatment with hucMSC-derived exosomes (hucMSC-Exos). H9C2 and human umbilical vascular endothelial cells (HUVECs) were subjected to oxygen and glucose deprivation and exosome treatment to quantify the cell proliferation and apoptosis in H9C2 cells and the tube formation capacity of the HUVECs. A dual-luciferase activity reporter assay was conducted to validate the interaction between miR-1246 and serine protease 23 (PRSS23). HucMSCs treatment led to a reduction in H9C2 apoptosis and an increase in HUVEC angiogenesis, which were mitigated when hucMSCs were treated with an miR-1246 inhibitor. We also confirmed that PRSS23 is a putative target of miR-1246 and that miR-1246 attenuated hypoxia-induced myocardial tissue damage by targeting PRSS23 and inhibiting the activation of the Snail/α-SMA signaling. Our findings suggest that exosomal miR-1246 from hucMSCs protects the heart from failure by targeting PRSS23. This article is protected by copyright. All rights reserved.
- Published
- 2021