1. The comet assay applied to cells of the eye
- Author
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Elisabeth Elje, Bjørn Nicolaissen, Amaya Azqueta, Elisa Rundén-Pran, Ingrida Smeringaiova, Andrew Collins, Katerina Jirsova, and Kristiane Haug Berg
- Subjects
0301 basic medicine ,Pathology ,medicine.medical_specialty ,genetic structures ,Endothelium ,DNA damage ,Health, Toxicology and Mutagenesis ,Lens Capsule, Crystalline ,Eye ,Toxicology ,03 medical and health sciences ,0302 clinical medicine ,Genetics ,medicine ,Animals ,Humans ,Iris (anatomy) ,Genetics (clinical) ,Corneal epithelium ,Retinal pigment epithelium ,Chemistry ,Epithelium, Corneal ,Endothelial Cells ,Environmental Exposure ,eye diseases ,Comet assay ,030104 developmental biology ,medicine.anatomical_structure ,030221 ophthalmology & optometry ,Tears ,Human eye ,Comet Assay ,sense organs ,DNA Damage ,Environmental Monitoring - Abstract
The human eye is relatively unexplored as a source of cells for investigating DNA damage. There have been some clinical studies, using cells from surgically removed tissues, and altered DNA bases as well as strand breaks have been measured using the comet assay. Tissues examined include corneal epithelium and endothelium, lens capsule, iris and retinal pigment epithelium. For the purpose of biomonitoring for exposure to potential mutagens in the environment, the eye-relatively unprotected as it is compared with the skin-would be a valuable object for study; non-invasive techniques exist to collect lachrymal duct cells from tears, or cells from the ocular surface by impression cytology, and these methods should be further developed and validated.
- Published
- 2017