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Pigment epithelial cells isolated from human peripheral iridectomies have limited properties of retinal stem cells
- Source :
- Acta Ophthalmologica. 89:e635-e644
- Publication Year :
- 2011
- Publisher :
- Wiley, 2011.
-
Abstract
- Purpose: The identification of cells with properties of retinal progenitor cells (RPCs) in the adult human ciliary margin (CM) prompted a number of studies of their proliferative and differentiation potential. One of the remaining challenges is to find a feasible method of isolating RPCs from the patient’s eye. In the human CM, only the iris pigment epithelium (IPE) is easily obtained by a minimally invasive procedure. In the light of recent studies questioning the existence of RPCs in the adult mammalian CM, we wanted to assess the potential of the adult human IPE as source of RPCs. Methods: The IPE were isolated from peripheral iridectomies during glaucoma surgery, and IPE and ciliary body (CB) epithelium were also isolated from post-mortem tissue. Cells were cultivated in sphere-promoting conditions or as monolayers. Whole-tissue samples, undifferentiated and differentiated cells were studied by immunocytochemistry, RT-PCR and transmission electron microscopy. Results: The adult human IPE, like the CB, expressed markers of RPCs such as Pax6, Sox2 and Nestin in vivo. Both sphere-promoting and monolayer cultures preserved this phenotype. However, both IPE ⁄ CB cultures expressed markers of differentiated epithelial cells such as Claudin, microphtalmia-associated transcription factor (MITF) and Cytokeratin-19. Ultrastructurally, IPE spheres displayed epithelial-like junctions and contained mature melanosomes. After induced differentiation, IPE-derived cells showed only partial neuronal differentiation expressing b-III-tubulin, Map-2 and Rhodopsin, whereas no mature glial markers were found. Conclusion: Proliferative cells with some properties of RPCs can be isolated from the adult human IPE by peripheral iridectomies. Yet, many cells retain properties of differentiated epithelial cells and lack central properties of somatic stem cells.
- Subjects :
- Adult
Iridectomy
Pathology
medicine.medical_specialty
Adolescent
Cellular differentiation
Iris
Cell Separation
Retinal Pigment Epithelium
Biology
Ciliary body
Microscopy, Electron, Transmission
SOX2
medicine
Humans
Iris pigment epithelium
Progenitor cell
Fluorescent Antibody Technique, Indirect
Pigment Epithelium of Eye
Cells, Cultured
Aged
Cell Proliferation
DNA Primers
Aged, 80 and over
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells
Ciliary Body
Glaucoma
General Medicine
Middle Aged
Nestin
Cell biology
Ophthalmology
medicine.anatomical_structure
sense organs
Stem cell
Biomarkers
Adult stem cell
Subjects
Details
- ISSN :
- 1755375X
- Volume :
- 89
- Database :
- OpenAIRE
- Journal :
- Acta Ophthalmologica
- Accession number :
- edsair.doi.dedup.....eff6bb5656c5dc82a08c5bb1c27c30d8